Acceleration of muscle regeneration by local injection of muscle‐specific microRNAs in rat skeletal muscle injury model
MicroRNA (miRNA)s are a class of non‐coding RNAs that regulate gene expression post‐transcriptionally. Muscle‐specific miRNA, miRNA (miR)‐1, miR‐133 and miR‐206 play a crucial role in the regulation of muscle development and homeostasis. Muscle injuries are a common muscloskeletal disorder, and the...
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| creator | Nakasa, Tomoyuki Ishikawa, Masakazu Shi, Ming Shibuya, Hayatoshi Adachi, Nobuo Ochi, Mitsuo |
| description | MicroRNA (miRNA)s are a class of non‐coding RNAs that regulate gene expression post‐transcriptionally. Muscle‐specific miRNA, miRNA (miR)‐1, miR‐133 and miR‐206 play a crucial role in the regulation of muscle development and homeostasis. Muscle injuries are a common muscloskeletal disorder, and the most effective treatment has not been established yet. The purpose of this study was to demonstrate that a local injection of double‐stranded (ds) miR‐1, miR‐133 and 206 can accelerate muscle regeneration in a rat skeletal muscle injury model. After the laceration of the rat tibialis anterior muscle, ds miR‐1, 133 and 206 mixture mediated atelocollagen was injected into the injured site. The control group was injected with control siRNA. At 1 week after injury, an injection of miRNAs could enhance muscle regeneration morphologically and physiologically, and prevent fibrosis effectively compared to the control siRNA. Administration of exogenous miR‐1, 133 and 206 can induce expression of myogenic markers, MyoD1, myogenin and Pax7 in mRNA and expression in the protein level at 3 and 7 days after injury. The combination of miR‐1, 133 and 206 can promote myotube differentiation, and the expression of MyoD1, myogenin and Pax7 were up‐regulated in C2C12 cells in vitro. Local injection of miR‐1, 133 and 206 could be a novel therapeutic strategy in the treatment of skeletal muscle injury. |
| doi_str_mv | 10.1111/j.1582-4934.2009.00898.x |
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Muscle‐specific miRNA, miRNA (miR)‐1, miR‐133 and miR‐206 play a crucial role in the regulation of muscle development and homeostasis. Muscle injuries are a common muscloskeletal disorder, and the most effective treatment has not been established yet. The purpose of this study was to demonstrate that a local injection of double‐stranded (ds) miR‐1, miR‐133 and 206 can accelerate muscle regeneration in a rat skeletal muscle injury model. After the laceration of the rat tibialis anterior muscle, ds miR‐1, 133 and 206 mixture mediated atelocollagen was injected into the injured site. The control group was injected with control siRNA. At 1 week after injury, an injection of miRNAs could enhance muscle regeneration morphologically and physiologically, and prevent fibrosis effectively compared to the control siRNA. Administration of exogenous miR‐1, 133 and 206 can induce expression of myogenic markers, MyoD1, myogenin and Pax7 in mRNA and expression in the protein level at 3 and 7 days after injury. The combination of miR‐1, 133 and 206 can promote myotube differentiation, and the expression of MyoD1, myogenin and Pax7 were up‐regulated in C2C12 cells in vitro. Local injection of miR‐1, 133 and 206 could be a novel therapeutic strategy in the treatment of skeletal muscle injury.</description><identifier>ISSN: 1582-1838</identifier><identifier>EISSN: 1582-4934</identifier><identifier>DOI: 10.1111/j.1582-4934.2009.00898.x</identifier><identifier>PMID: 19754672</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Atelocollagen ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Down-Regulation ; Fibrosis ; Gene expression ; Homeostasis ; Immunohistochemistry ; Injection ; Ketamine ; Legs ; microRNA ; MicroRNAs ; MicroRNAs - metabolism ; miRNA ; Models, Animal ; Morphology ; muscle ; Muscle Development ; Muscle, Skeletal - growth & development ; Muscle, Skeletal - injuries ; Musculoskeletal diseases ; Musculoskeletal system ; MyoD Protein - genetics ; MyoD Protein - metabolism ; MyoD1 ; Myogenin ; Myogenin - genetics ; Myogenin - metabolism ; Myostatin - genetics ; Myostatin - metabolism ; Myotubes ; Paired Box Transcription Factors - genetics ; Paired Box Transcription Factors - metabolism ; Pathogenesis ; Pax7 ; Penicillin ; Post-transcription ; Proteins ; Random Allocation ; Rats ; Regeneration ; Rodents ; siRNA ; Skeletal muscle ; Studies ; Tensile strength ; Tibialis anterior muscle ; Up-Regulation</subject><ispartof>Journal of cellular and molecular medicine, 2010-10, Vol.14 (10), p.2495-2505</ispartof><rights>2009 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd</rights><rights>2009 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.</rights><rights>2010. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright Blackwell Publishing Ltd. Oct 2010</rights><rights>2009 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5948-670b0a73eaf1e5fc3ac9cc52ce7de88cf0cd0a11339962815f2e929a611d01493</citedby><cites>FETCH-LOGICAL-c5948-670b0a73eaf1e5fc3ac9cc52ce7de88cf0cd0a11339962815f2e929a611d01493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823166/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3823166/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1582-4934.2009.00898.x$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19754672$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakasa, Tomoyuki</creatorcontrib><creatorcontrib>Ishikawa, Masakazu</creatorcontrib><creatorcontrib>Shi, Ming</creatorcontrib><creatorcontrib>Shibuya, Hayatoshi</creatorcontrib><creatorcontrib>Adachi, Nobuo</creatorcontrib><creatorcontrib>Ochi, Mitsuo</creatorcontrib><title>Acceleration of muscle regeneration by local injection of muscle‐specific microRNAs in rat skeletal muscle injury model</title><title>Journal of cellular and molecular medicine</title><addtitle>J Cell Mol Med</addtitle><description>MicroRNA (miRNA)s are a class of non‐coding RNAs that regulate gene expression post‐transcriptionally. Muscle‐specific miRNA, miRNA (miR)‐1, miR‐133 and miR‐206 play a crucial role in the regulation of muscle development and homeostasis. Muscle injuries are a common muscloskeletal disorder, and the most effective treatment has not been established yet. The purpose of this study was to demonstrate that a local injection of double‐stranded (ds) miR‐1, miR‐133 and 206 can accelerate muscle regeneration in a rat skeletal muscle injury model. After the laceration of the rat tibialis anterior muscle, ds miR‐1, 133 and 206 mixture mediated atelocollagen was injected into the injured site. The control group was injected with control siRNA. At 1 week after injury, an injection of miRNAs could enhance muscle regeneration morphologically and physiologically, and prevent fibrosis effectively compared to the control siRNA. Administration of exogenous miR‐1, 133 and 206 can induce expression of myogenic markers, MyoD1, myogenin and Pax7 in mRNA and expression in the protein level at 3 and 7 days after injury. The combination of miR‐1, 133 and 206 can promote myotube differentiation, and the expression of MyoD1, myogenin and Pax7 were up‐regulated in C2C12 cells in vitro. Local injection of miR‐1, 133 and 206 could be a novel therapeutic strategy in the treatment of skeletal muscle injury.</description><subject>Animals</subject><subject>Atelocollagen</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Down-Regulation</subject><subject>Fibrosis</subject><subject>Gene expression</subject><subject>Homeostasis</subject><subject>Immunohistochemistry</subject><subject>Injection</subject><subject>Ketamine</subject><subject>Legs</subject><subject>microRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>Models, Animal</subject><subject>Morphology</subject><subject>muscle</subject><subject>Muscle Development</subject><subject>Muscle, Skeletal - growth & development</subject><subject>Muscle, Skeletal - injuries</subject><subject>Musculoskeletal diseases</subject><subject>Musculoskeletal system</subject><subject>MyoD Protein - genetics</subject><subject>MyoD Protein - metabolism</subject><subject>MyoD1</subject><subject>Myogenin</subject><subject>Myogenin - genetics</subject><subject>Myogenin - metabolism</subject><subject>Myostatin - genetics</subject><subject>Myostatin - metabolism</subject><subject>Myotubes</subject><subject>Paired Box Transcription Factors - genetics</subject><subject>Paired Box Transcription Factors - metabolism</subject><subject>Pathogenesis</subject><subject>Pax7</subject><subject>Penicillin</subject><subject>Post-transcription</subject><subject>Proteins</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Regeneration</subject><subject>Rodents</subject><subject>siRNA</subject><subject>Skeletal muscle</subject><subject>Studies</subject><subject>Tensile strength</subject><subject>Tibialis anterior muscle</subject><subject>Up-Regulation</subject><issn>1582-1838</issn><issn>1582-4934</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkstu1DAUhi0Eou3AKyALFqwm-BIn9gKk0aiFohYkBGvL45wUhySe2hPa7HgEnpEnwemEQpGQ8MaWz_f_OjeEMCUZTedFk1Eh2TJXPM8YISojRCqZXd9Dh7eB-_ObSi4P0FGMDSG8oFw9RAdUlSIvSnaIxpW10EIwO-d77GvcDdG2gANcQP_rezPi1lvTYtc3YO-SP759j1uwrnYWd84G_-HdKiYQJy2OX5L3Lgln16Qfwog7X0H7CD2oTRvh8Xwv0KeT44_rN8uz969P16uzpRUql8uiJBtiSg6mpiBqy41V1gpmoaxASlsTWxFDKedKFUxSUTNQTJmC0orQ1IgFerX33Q6bDioL_S6YVm-D60wYtTdO34307rO-8F81l4zTokgGz2eD4C8HiDvduZia1poe_BB1WVCSc5YyWKCnf5GNH0KfqtOSyFzlXE12z_4FcVIKJSQvRaLknkodjTFAfZswJXraAd3oabx6GrWedkDf7IC-TtInfxb8WzgPPQEv98CVa2H8b2P9dn1-nl78J0qSwyE</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Nakasa, Tomoyuki</creator><creator>Ishikawa, Masakazu</creator><creator>Shi, Ming</creator><creator>Shibuya, Hayatoshi</creator><creator>Adachi, Nobuo</creator><creator>Ochi, Mitsuo</creator><general>Blackwell Publishing Ltd</general><general>John Wiley & Sons, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201010</creationdate><title>Acceleration of muscle regeneration by local injection of muscle‐specific microRNAs in rat skeletal muscle injury model</title><author>Nakasa, Tomoyuki ; Ishikawa, Masakazu ; Shi, Ming ; Shibuya, Hayatoshi ; Adachi, Nobuo ; Ochi, Mitsuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5948-670b0a73eaf1e5fc3ac9cc52ce7de88cf0cd0a11339962815f2e929a611d01493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Atelocollagen</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Down-Regulation</topic><topic>Fibrosis</topic><topic>Gene expression</topic><topic>Homeostasis</topic><topic>Immunohistochemistry</topic><topic>Injection</topic><topic>Ketamine</topic><topic>Legs</topic><topic>microRNA</topic><topic>MicroRNAs</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>Models, Animal</topic><topic>Morphology</topic><topic>muscle</topic><topic>Muscle Development</topic><topic>Muscle, Skeletal - growth & development</topic><topic>Muscle, Skeletal - injuries</topic><topic>Musculoskeletal diseases</topic><topic>Musculoskeletal system</topic><topic>MyoD Protein - genetics</topic><topic>MyoD Protein - metabolism</topic><topic>MyoD1</topic><topic>Myogenin</topic><topic>Myogenin - genetics</topic><topic>Myogenin - metabolism</topic><topic>Myostatin - genetics</topic><topic>Myostatin - metabolism</topic><topic>Myotubes</topic><topic>Paired Box Transcription Factors - genetics</topic><topic>Paired Box Transcription Factors - metabolism</topic><topic>Pathogenesis</topic><topic>Pax7</topic><topic>Penicillin</topic><topic>Post-transcription</topic><topic>Proteins</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Regeneration</topic><topic>Rodents</topic><topic>siRNA</topic><topic>Skeletal muscle</topic><topic>Studies</topic><topic>Tensile strength</topic><topic>Tibialis anterior muscle</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakasa, Tomoyuki</creatorcontrib><creatorcontrib>Ishikawa, Masakazu</creatorcontrib><creatorcontrib>Shi, Ming</creatorcontrib><creatorcontrib>Shibuya, Hayatoshi</creatorcontrib><creatorcontrib>Adachi, Nobuo</creatorcontrib><creatorcontrib>Ochi, Mitsuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of cellular and molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Nakasa, Tomoyuki</au><au>Ishikawa, Masakazu</au><au>Shi, Ming</au><au>Shibuya, Hayatoshi</au><au>Adachi, Nobuo</au><au>Ochi, Mitsuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acceleration of muscle regeneration by local injection of muscle‐specific microRNAs in rat skeletal muscle injury model</atitle><jtitle>Journal of cellular and molecular medicine</jtitle><addtitle>J Cell Mol Med</addtitle><date>2010-10</date><risdate>2010</risdate><volume>14</volume><issue>10</issue><spage>2495</spage><epage>2505</epage><pages>2495-2505</pages><issn>1582-1838</issn><eissn>1582-4934</eissn><abstract>MicroRNA (miRNA)s are a class of non‐coding RNAs that regulate gene expression post‐transcriptionally. Muscle‐specific miRNA, miRNA (miR)‐1, miR‐133 and miR‐206 play a crucial role in the regulation of muscle development and homeostasis. Muscle injuries are a common muscloskeletal disorder, and the most effective treatment has not been established yet. The purpose of this study was to demonstrate that a local injection of double‐stranded (ds) miR‐1, miR‐133 and 206 can accelerate muscle regeneration in a rat skeletal muscle injury model. After the laceration of the rat tibialis anterior muscle, ds miR‐1, 133 and 206 mixture mediated atelocollagen was injected into the injured site. The control group was injected with control siRNA. At 1 week after injury, an injection of miRNAs could enhance muscle regeneration morphologically and physiologically, and prevent fibrosis effectively compared to the control siRNA. Administration of exogenous miR‐1, 133 and 206 can induce expression of myogenic markers, MyoD1, myogenin and Pax7 in mRNA and expression in the protein level at 3 and 7 days after injury. The combination of miR‐1, 133 and 206 can promote myotube differentiation, and the expression of MyoD1, myogenin and Pax7 were up‐regulated in C2C12 cells in vitro. Local injection of miR‐1, 133 and 206 could be a novel therapeutic strategy in the treatment of skeletal muscle injury.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19754672</pmid><doi>10.1111/j.1582-4934.2009.00898.x</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Atelocollagen Cell Differentiation Cell Proliferation Cells, Cultured Down-Regulation Fibrosis Gene expression Homeostasis Immunohistochemistry Injection Ketamine Legs microRNA MicroRNAs MicroRNAs - metabolism miRNA Models, Animal Morphology muscle Muscle Development Muscle, Skeletal - growth & development Muscle, Skeletal - injuries Musculoskeletal diseases Musculoskeletal system MyoD Protein - genetics MyoD Protein - metabolism MyoD1 Myogenin Myogenin - genetics Myogenin - metabolism Myostatin - genetics Myostatin - metabolism Myotubes Paired Box Transcription Factors - genetics Paired Box Transcription Factors - metabolism Pathogenesis Pax7 Penicillin Post-transcription Proteins Random Allocation Rats Regeneration Rodents siRNA Skeletal muscle Studies Tensile strength Tibialis anterior muscle Up-Regulation |
| title | Acceleration of muscle regeneration by local injection of muscle‐specific microRNAs in rat skeletal muscle injury model |
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