Engineered heart tissue graft derived from somatic cell nuclear transferred embryonic stem cells improve myocardial performance in infarcted rat heart
The concept of regenerating diseased myocardium by implanting engineered heart tissue (EHT) is intriguing. Yet it was limited by immune rejection and difficulties to be generated at a size with contractile properties. Somatic cell nuclear transfer is proposed as a practical strategy for generating a...
Gespeichert in:
| Veröffentlicht in: | Journal of cellular and molecular medicine 2010-12, Vol.14 (12), p.2771-2779 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2779 |
|---|---|
| container_issue | 12 |
| container_start_page | 2771 |
| container_title | Journal of cellular and molecular medicine |
| container_volume | 14 |
| creator | Lü, Shuanghong Li, Ying Gao, Shaorong Liu, Sheng Wang, Haibin He, Wenjun Zhou, Jin Liu, Zhiqiang Zhang, Ye Lin, Qiuxia Duan, Cumi Yang, Xiangzhong (Jerry) Wang, Changyong |
| description | The concept of regenerating diseased myocardium by implanting engineered heart tissue (EHT) is intriguing. Yet it was limited by immune rejection and difficulties to be generated at a size with contractile properties. Somatic cell nuclear transfer is proposed as a practical strategy for generating autologous histocompatible stem (nuclear transferred embryonic stem [NT‐ES]) cells to treat diseases. Nevertheless, it is controversial as NT‐ES cells may pose risks in their therapeutic application. EHT from NT‐ES cell‐derived cardiomyocytes was generated through a series of improved techniques in a self‐made mould to keep the EHTs from contraction and provide static stretch simultaneously. After 7 days of static and mechanical stretching, respectively, the EHTs were implanted to the infarcted rat heart. Four weeks after transplantation, the suitability of EHT in heart muscle repair after myocardial infarction was evaluated by histological examination, echocardiography and multielectrode array measurement. The results showed that large (thickness/diameter, 2–4 mm/10 mm) spontaneously contracting EHTs was generated successfully. The EHTs, which were derived from NT‐ES cells, inte grated and electrically coupled to host myocardium and exerted beneficial effects on the left ventricular function of infarcted rat heart. No teratoma formation was observed in the rat heart implanted with EHTs for 4 weeks. NT‐ES cells can be used as a source of seeding cells for cardiac tissue engineering. Large contractile EHT grafts can be constructed in vitro with the ability to survive after implantation and improve myocardial performance of infarcted rat hearts. |
| doi_str_mv | 10.1111/j.1582-4934.2010.01112.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_24P</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3822727</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2247847401</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5292-cee8af60e4ab277a443e3b7a06c1c8f4d8d06a8e1461e9478e6c7b12c91ee8163</originalsourceid><addsrcrecordid>eNqNkdtuEzEQhlcIREvhFZAF1wk-7MF7g4SiUkCtuIFry-sdp47Wdhjvps2L8Lz1NiHABRLWSB7NfPNrRn9REEaXLL93myWrJF-UrSiXnOYqzVW-vH9SnJ8aT485k0KeFS9S2lAqaiba58UZp5WspaDnxc_LsHYBAKEnt6BxJKNLaQKyRm1H0gO6XW5ZjJ6k6PXoDDEwDCRMZsg8GVGHZAFnAfAd7mPISBrBP3KJOL_FuAPi99Fo7J0eyBbQRvQ6GCAu5LAazZgFUI-HLV4Wz6weErw6_hfF94-X31afFtdfrz6vPlwvTMVbvjAAUtuaQqk73jS6LAWIrtG0NsxIW_ayp7WWwMqaQVs2EmrTdIybluVJVouL4v1Bdzt1HnoDId8zqC06r3Gvonbq705wt2odd0pIzhveZIE3RwGMPyZIo9rECUPeWcmyYRVjrczQ239BgjZVW8mWt5mSB8pgTAnBntZgVM22q42aHVWzu2q2XT3aru7z6Os_zzgN_vL59513boD9fwurL6ubmzkVD1ZEwEY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3075958929</pqid></control><display><type>article</type><title>Engineered heart tissue graft derived from somatic cell nuclear transferred embryonic stem cells improve myocardial performance in infarcted rat heart</title><source>Wiley Online Library</source><creator>Lü, Shuanghong ; Li, Ying ; Gao, Shaorong ; Liu, Sheng ; Wang, Haibin ; He, Wenjun ; Zhou, Jin ; Liu, Zhiqiang ; Zhang, Ye ; Lin, Qiuxia ; Duan, Cumi ; Yang, Xiangzhong (Jerry) ; Wang, Changyong</creator><creatorcontrib>Lü, Shuanghong ; Li, Ying ; Gao, Shaorong ; Liu, Sheng ; Wang, Haibin ; He, Wenjun ; Zhou, Jin ; Liu, Zhiqiang ; Zhang, Ye ; Lin, Qiuxia ; Duan, Cumi ; Yang, Xiangzhong (Jerry) ; Wang, Changyong</creatorcontrib><description>The concept of regenerating diseased myocardium by implanting engineered heart tissue (EHT) is intriguing. Yet it was limited by immune rejection and difficulties to be generated at a size with contractile properties. Somatic cell nuclear transfer is proposed as a practical strategy for generating autologous histocompatible stem (nuclear transferred embryonic stem [NT‐ES]) cells to treat diseases. Nevertheless, it is controversial as NT‐ES cells may pose risks in their therapeutic application. EHT from NT‐ES cell‐derived cardiomyocytes was generated through a series of improved techniques in a self‐made mould to keep the EHTs from contraction and provide static stretch simultaneously. After 7 days of static and mechanical stretching, respectively, the EHTs were implanted to the infarcted rat heart. Four weeks after transplantation, the suitability of EHT in heart muscle repair after myocardial infarction was evaluated by histological examination, echocardiography and multielectrode array measurement. The results showed that large (thickness/diameter, 2–4 mm/10 mm) spontaneously contracting EHTs was generated successfully. The EHTs, which were derived from NT‐ES cells, inte grated and electrically coupled to host myocardium and exerted beneficial effects on the left ventricular function of infarcted rat heart. No teratoma formation was observed in the rat heart implanted with EHTs for 4 weeks. NT‐ES cells can be used as a source of seeding cells for cardiac tissue engineering. Large contractile EHT grafts can be constructed in vitro with the ability to survive after implantation and improve myocardial performance of infarcted rat hearts.</description><identifier>ISSN: 1582-1838</identifier><identifier>EISSN: 1582-4934</identifier><identifier>DOI: 10.1111/j.1582-4934.2010.01112.x</identifier><identifier>PMID: 20586830</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Cardiac muscle ; Cardiomyocytes ; Cell culture ; Cell size ; Cloning ; Coronary vessels ; Echocardiography ; Electrocardiography ; Embryo cells ; embryonic stem cells ; Embryonic Stem Cells - cytology ; Graft rejection ; Heart - physiology ; Heart attacks ; Heart diseases ; Immunology ; Medical treatment ; Mice ; Muscle contraction ; Myocardial Contraction ; Myocardial infarction ; Myocardial Infarction - surgery ; Myocardium ; Myocytes, Cardiac - physiology ; Nuclear transfer ; Nuclear Transfer Techniques ; Ostomy ; Penicillin ; Rats ; Regeneration ; Rodents ; Somatic cell nuclear transfer ; Stem cells ; Teratoma ; Tissue engineering ; Tissue Engineering - methods ; Tissue Transplantation ; Transplantation, Autologous ; Transplants & implants</subject><ispartof>Journal of cellular and molecular medicine, 2010-12, Vol.14 (12), p.2771-2779</ispartof><rights>2010 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd</rights><rights>2010 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.</rights><rights>2010. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright Blackwell Publishing Ltd. Dec 2010</rights><rights>2010 The Authors Journal compilation © 2010 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5292-cee8af60e4ab277a443e3b7a06c1c8f4d8d06a8e1461e9478e6c7b12c91ee8163</citedby><cites>FETCH-LOGICAL-c5292-cee8af60e4ab277a443e3b7a06c1c8f4d8d06a8e1461e9478e6c7b12c91ee8163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822727/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822727/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1582-4934.2010.01112.x$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20586830$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lü, Shuanghong</creatorcontrib><creatorcontrib>Li, Ying</creatorcontrib><creatorcontrib>Gao, Shaorong</creatorcontrib><creatorcontrib>Liu, Sheng</creatorcontrib><creatorcontrib>Wang, Haibin</creatorcontrib><creatorcontrib>He, Wenjun</creatorcontrib><creatorcontrib>Zhou, Jin</creatorcontrib><creatorcontrib>Liu, Zhiqiang</creatorcontrib><creatorcontrib>Zhang, Ye</creatorcontrib><creatorcontrib>Lin, Qiuxia</creatorcontrib><creatorcontrib>Duan, Cumi</creatorcontrib><creatorcontrib>Yang, Xiangzhong (Jerry)</creatorcontrib><creatorcontrib>Wang, Changyong</creatorcontrib><title>Engineered heart tissue graft derived from somatic cell nuclear transferred embryonic stem cells improve myocardial performance in infarcted rat heart</title><title>Journal of cellular and molecular medicine</title><addtitle>J Cell Mol Med</addtitle><description>The concept of regenerating diseased myocardium by implanting engineered heart tissue (EHT) is intriguing. Yet it was limited by immune rejection and difficulties to be generated at a size with contractile properties. Somatic cell nuclear transfer is proposed as a practical strategy for generating autologous histocompatible stem (nuclear transferred embryonic stem [NT‐ES]) cells to treat diseases. Nevertheless, it is controversial as NT‐ES cells may pose risks in their therapeutic application. EHT from NT‐ES cell‐derived cardiomyocytes was generated through a series of improved techniques in a self‐made mould to keep the EHTs from contraction and provide static stretch simultaneously. After 7 days of static and mechanical stretching, respectively, the EHTs were implanted to the infarcted rat heart. Four weeks after transplantation, the suitability of EHT in heart muscle repair after myocardial infarction was evaluated by histological examination, echocardiography and multielectrode array measurement. The results showed that large (thickness/diameter, 2–4 mm/10 mm) spontaneously contracting EHTs was generated successfully. The EHTs, which were derived from NT‐ES cells, inte grated and electrically coupled to host myocardium and exerted beneficial effects on the left ventricular function of infarcted rat heart. No teratoma formation was observed in the rat heart implanted with EHTs for 4 weeks. NT‐ES cells can be used as a source of seeding cells for cardiac tissue engineering. Large contractile EHT grafts can be constructed in vitro with the ability to survive after implantation and improve myocardial performance of infarcted rat hearts.</description><subject>Animals</subject><subject>Cardiac muscle</subject><subject>Cardiomyocytes</subject><subject>Cell culture</subject><subject>Cell size</subject><subject>Cloning</subject><subject>Coronary vessels</subject><subject>Echocardiography</subject><subject>Electrocardiography</subject><subject>Embryo cells</subject><subject>embryonic stem cells</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Graft rejection</subject><subject>Heart - physiology</subject><subject>Heart attacks</subject><subject>Heart diseases</subject><subject>Immunology</subject><subject>Medical treatment</subject><subject>Mice</subject><subject>Muscle contraction</subject><subject>Myocardial Contraction</subject><subject>Myocardial infarction</subject><subject>Myocardial Infarction - surgery</subject><subject>Myocardium</subject><subject>Myocytes, Cardiac - physiology</subject><subject>Nuclear transfer</subject><subject>Nuclear Transfer Techniques</subject><subject>Ostomy</subject><subject>Penicillin</subject><subject>Rats</subject><subject>Regeneration</subject><subject>Rodents</subject><subject>Somatic cell nuclear transfer</subject><subject>Stem cells</subject><subject>Teratoma</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Transplantation</subject><subject>Transplantation, Autologous</subject><subject>Transplants & implants</subject><issn>1582-1838</issn><issn>1582-4934</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkdtuEzEQhlcIREvhFZAF1wk-7MF7g4SiUkCtuIFry-sdp47Wdhjvps2L8Lz1NiHABRLWSB7NfPNrRn9REEaXLL93myWrJF-UrSiXnOYqzVW-vH9SnJ8aT485k0KeFS9S2lAqaiba58UZp5WspaDnxc_LsHYBAKEnt6BxJKNLaQKyRm1H0gO6XW5ZjJ6k6PXoDDEwDCRMZsg8GVGHZAFnAfAd7mPISBrBP3KJOL_FuAPi99Fo7J0eyBbQRvQ6GCAu5LAazZgFUI-HLV4Wz6weErw6_hfF94-X31afFtdfrz6vPlwvTMVbvjAAUtuaQqk73jS6LAWIrtG0NsxIW_ayp7WWwMqaQVs2EmrTdIybluVJVouL4v1Bdzt1HnoDId8zqC06r3Gvonbq705wt2odd0pIzhveZIE3RwGMPyZIo9rECUPeWcmyYRVjrczQ239BgjZVW8mWt5mSB8pgTAnBntZgVM22q42aHVWzu2q2XT3aru7z6Os_zzgN_vL59513boD9fwurL6ubmzkVD1ZEwEY</recordid><startdate>201012</startdate><enddate>201012</enddate><creator>Lü, Shuanghong</creator><creator>Li, Ying</creator><creator>Gao, Shaorong</creator><creator>Liu, Sheng</creator><creator>Wang, Haibin</creator><creator>He, Wenjun</creator><creator>Zhou, Jin</creator><creator>Liu, Zhiqiang</creator><creator>Zhang, Ye</creator><creator>Lin, Qiuxia</creator><creator>Duan, Cumi</creator><creator>Yang, Xiangzhong (Jerry)</creator><creator>Wang, Changyong</creator><general>Blackwell Publishing Ltd</general><general>John Wiley & Sons, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>201012</creationdate><title>Engineered heart tissue graft derived from somatic cell nuclear transferred embryonic stem cells improve myocardial performance in infarcted rat heart</title><author>Lü, Shuanghong ; Li, Ying ; Gao, Shaorong ; Liu, Sheng ; Wang, Haibin ; He, Wenjun ; Zhou, Jin ; Liu, Zhiqiang ; Zhang, Ye ; Lin, Qiuxia ; Duan, Cumi ; Yang, Xiangzhong (Jerry) ; Wang, Changyong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5292-cee8af60e4ab277a443e3b7a06c1c8f4d8d06a8e1461e9478e6c7b12c91ee8163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Cardiac muscle</topic><topic>Cardiomyocytes</topic><topic>Cell culture</topic><topic>Cell size</topic><topic>Cloning</topic><topic>Coronary vessels</topic><topic>Echocardiography</topic><topic>Electrocardiography</topic><topic>Embryo cells</topic><topic>embryonic stem cells</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Graft rejection</topic><topic>Heart - physiology</topic><topic>Heart attacks</topic><topic>Heart diseases</topic><topic>Immunology</topic><topic>Medical treatment</topic><topic>Mice</topic><topic>Muscle contraction</topic><topic>Myocardial Contraction</topic><topic>Myocardial infarction</topic><topic>Myocardial Infarction - surgery</topic><topic>Myocardium</topic><topic>Myocytes, Cardiac - physiology</topic><topic>Nuclear transfer</topic><topic>Nuclear Transfer Techniques</topic><topic>Ostomy</topic><topic>Penicillin</topic><topic>Rats</topic><topic>Regeneration</topic><topic>Rodents</topic><topic>Somatic cell nuclear transfer</topic><topic>Stem cells</topic><topic>Teratoma</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Transplantation</topic><topic>Transplantation, Autologous</topic><topic>Transplants & implants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lü, Shuanghong</creatorcontrib><creatorcontrib>Li, Ying</creatorcontrib><creatorcontrib>Gao, Shaorong</creatorcontrib><creatorcontrib>Liu, Sheng</creatorcontrib><creatorcontrib>Wang, Haibin</creatorcontrib><creatorcontrib>He, Wenjun</creatorcontrib><creatorcontrib>Zhou, Jin</creatorcontrib><creatorcontrib>Liu, Zhiqiang</creatorcontrib><creatorcontrib>Zhang, Ye</creatorcontrib><creatorcontrib>Lin, Qiuxia</creatorcontrib><creatorcontrib>Duan, Cumi</creatorcontrib><creatorcontrib>Yang, Xiangzhong (Jerry)</creatorcontrib><creatorcontrib>Wang, Changyong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of cellular and molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Lü, Shuanghong</au><au>Li, Ying</au><au>Gao, Shaorong</au><au>Liu, Sheng</au><au>Wang, Haibin</au><au>He, Wenjun</au><au>Zhou, Jin</au><au>Liu, Zhiqiang</au><au>Zhang, Ye</au><au>Lin, Qiuxia</au><au>Duan, Cumi</au><au>Yang, Xiangzhong (Jerry)</au><au>Wang, Changyong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Engineered heart tissue graft derived from somatic cell nuclear transferred embryonic stem cells improve myocardial performance in infarcted rat heart</atitle><jtitle>Journal of cellular and molecular medicine</jtitle><addtitle>J Cell Mol Med</addtitle><date>2010-12</date><risdate>2010</risdate><volume>14</volume><issue>12</issue><spage>2771</spage><epage>2779</epage><pages>2771-2779</pages><issn>1582-1838</issn><eissn>1582-4934</eissn><abstract>The concept of regenerating diseased myocardium by implanting engineered heart tissue (EHT) is intriguing. Yet it was limited by immune rejection and difficulties to be generated at a size with contractile properties. Somatic cell nuclear transfer is proposed as a practical strategy for generating autologous histocompatible stem (nuclear transferred embryonic stem [NT‐ES]) cells to treat diseases. Nevertheless, it is controversial as NT‐ES cells may pose risks in their therapeutic application. EHT from NT‐ES cell‐derived cardiomyocytes was generated through a series of improved techniques in a self‐made mould to keep the EHTs from contraction and provide static stretch simultaneously. After 7 days of static and mechanical stretching, respectively, the EHTs were implanted to the infarcted rat heart. Four weeks after transplantation, the suitability of EHT in heart muscle repair after myocardial infarction was evaluated by histological examination, echocardiography and multielectrode array measurement. The results showed that large (thickness/diameter, 2–4 mm/10 mm) spontaneously contracting EHTs was generated successfully. The EHTs, which were derived from NT‐ES cells, inte grated and electrically coupled to host myocardium and exerted beneficial effects on the left ventricular function of infarcted rat heart. No teratoma formation was observed in the rat heart implanted with EHTs for 4 weeks. NT‐ES cells can be used as a source of seeding cells for cardiac tissue engineering. Large contractile EHT grafts can be constructed in vitro with the ability to survive after implantation and improve myocardial performance of infarcted rat hearts.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20586830</pmid><doi>10.1111/j.1582-4934.2010.01112.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | ISSN: 1582-1838 |
| ispartof | Journal of cellular and molecular medicine, 2010-12, Vol.14 (12), p.2771-2779 |
| issn | 1582-1838 1582-4934 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3822727 |
| source | Wiley Online Library |
| subjects | Animals Cardiac muscle Cardiomyocytes Cell culture Cell size Cloning Coronary vessels Echocardiography Electrocardiography Embryo cells embryonic stem cells Embryonic Stem Cells - cytology Graft rejection Heart - physiology Heart attacks Heart diseases Immunology Medical treatment Mice Muscle contraction Myocardial Contraction Myocardial infarction Myocardial Infarction - surgery Myocardium Myocytes, Cardiac - physiology Nuclear transfer Nuclear Transfer Techniques Ostomy Penicillin Rats Regeneration Rodents Somatic cell nuclear transfer Stem cells Teratoma Tissue engineering Tissue Engineering - methods Tissue Transplantation Transplantation, Autologous Transplants & implants |
| title | Engineered heart tissue graft derived from somatic cell nuclear transferred embryonic stem cells improve myocardial performance in infarcted rat heart |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T18%3A11%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_24P&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Engineered%20heart%20tissue%20graft%20derived%20from%20somatic%20cell%20nuclear%20transferred%20embryonic%20stem%20cells%20improve%20myocardial%20performance%20in%20infarcted%20rat%20heart&rft.jtitle=Journal%20of%20cellular%20and%20molecular%20medicine&rft.au=L%C3%BC,%20Shuanghong&rft.date=2010-12&rft.volume=14&rft.issue=12&rft.spage=2771&rft.epage=2779&rft.pages=2771-2779&rft.issn=1582-1838&rft.eissn=1582-4934&rft_id=info:doi/10.1111/j.1582-4934.2010.01112.x&rft_dat=%3Cproquest_24P%3E2247847401%3C/proquest_24P%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3075958929&rft_id=info:pmid/20586830&rfr_iscdi=true |