Potential role of oxidative exoenzymes of the extremophilic fungus Pestalotiopsis palmarum BM‐04 in biotransformation of extra‐heavy crude oil
Summary Large amount of drilling waste associated with the expansion of the Orinoco Oil Belt (OOB), the biggest proven reserve of extra‐heavy crude oil (EHCO) worldwide, is usually impregnated with EHCO and highly salinized water‐based drilling fluids. Oxidative exoenzymes (OE) of the lignin‐degradi...
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| creator | Naranjo‐Briceño, Leopoldo Pernía, Beatriz Guerra, Mayamaru Demey, Jhonny R. Sisto, Ángela Inojosa, Ysvic González, Meralys Fusella, Emidio Freites, Miguel Yegres, Francisco |
| description | Summary
Large amount of drilling waste associated with the expansion of the Orinoco Oil Belt (OOB), the biggest proven reserve of extra‐heavy crude oil (EHCO) worldwide, is usually impregnated with EHCO and highly salinized water‐based drilling fluids. Oxidative exoenzymes (OE) of the lignin‐degrading enzyme system (LDS) of fungi catalyse the oxidation of a wide range of toxic pollutants. However, very little evidences on fungal degradation or biotransformation of EHCO have been reported, which contain high amounts of asphaltenes and its biodegradation rate is very limited. The aims of this work were to study the ability of Pestalotiopsis palmarum BM‐04 to synthesize OE, its potential to biotransform EHCO and to survive in extreme environmental conditions. Enzymatic studies of the LDS showed the ability of this fungus to overproduce high amounts of laccase (LACp) in presence of wheat bran or lignin peroxidase (LIPp) with EHCO as sole carbon and energy source (1300 U mgP−1 in both cases). FT‐IR spectroscopy with Attenuated Total Reflectance (ATR) analysis showed the enzymatic oxidation of carbon and sulfur atoms in both maltenes and asphaltenes fractions of biotreated EHCO catalysed by cell‐free laccase‐enriched OE using wheat bran as inducer. UV‐visible spectrophotometry analysis revealed the oxidation of the petroporphyrins in the asphaltenes fraction of biotreated EHCO. Tolerance assays showed the ability of this fungus to grow up to 50 000 p.p.m. of EHCO and 2000 mM of NaCl. These results suggest that P. palmarum BM‐04 is a hopeful alternative to be used in remediation processes in extreme environmental conditions of salinity and EHCO contamination, such as the drilling waste from the OOB.
Oxidative exoenzymes (OE) of the lignin‐degrading enzyme system (LDS) of fungi catalyze the oxidation of a wide range of toxic pollutants. However, very little evidences on fungal degradation or biotransformation of extra‐heavy crude oil (EHCO) have been reported. The aims of this work were to study the ability of Pestalotiopsis palmarum BM‐04 to synthesize OE, its potential to biotransform EHCO and to survive in extreme environmental conditions. Enzymatic studies of the LDS showed the ability of this fungus to overproduce high amounts of laccase (LACp) or lignin peroxidase (LIPp) in presence of wheat bran or EHCO as sole carbon and energy source, respectively. FT‐IR‐ATR spectroscopy analysis showed the enzymatic oxidation of carbon and sulfur atoms in both maltenes a |
| doi_str_mv | 10.1111/1751-7915.12067 |
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Large amount of drilling waste associated with the expansion of the Orinoco Oil Belt (OOB), the biggest proven reserve of extra‐heavy crude oil (EHCO) worldwide, is usually impregnated with EHCO and highly salinized water‐based drilling fluids. Oxidative exoenzymes (OE) of the lignin‐degrading enzyme system (LDS) of fungi catalyse the oxidation of a wide range of toxic pollutants. However, very little evidences on fungal degradation or biotransformation of EHCO have been reported, which contain high amounts of asphaltenes and its biodegradation rate is very limited. The aims of this work were to study the ability of Pestalotiopsis palmarum BM‐04 to synthesize OE, its potential to biotransform EHCO and to survive in extreme environmental conditions. Enzymatic studies of the LDS showed the ability of this fungus to overproduce high amounts of laccase (LACp) in presence of wheat bran or lignin peroxidase (LIPp) with EHCO as sole carbon and energy source (1300 U mgP−1 in both cases). FT‐IR spectroscopy with Attenuated Total Reflectance (ATR) analysis showed the enzymatic oxidation of carbon and sulfur atoms in both maltenes and asphaltenes fractions of biotreated EHCO catalysed by cell‐free laccase‐enriched OE using wheat bran as inducer. UV‐visible spectrophotometry analysis revealed the oxidation of the petroporphyrins in the asphaltenes fraction of biotreated EHCO. Tolerance assays showed the ability of this fungus to grow up to 50 000 p.p.m. of EHCO and 2000 mM of NaCl. These results suggest that P. palmarum BM‐04 is a hopeful alternative to be used in remediation processes in extreme environmental conditions of salinity and EHCO contamination, such as the drilling waste from the OOB.
Oxidative exoenzymes (OE) of the lignin‐degrading enzyme system (LDS) of fungi catalyze the oxidation of a wide range of toxic pollutants. However, very little evidences on fungal degradation or biotransformation of extra‐heavy crude oil (EHCO) have been reported. The aims of this work were to study the ability of Pestalotiopsis palmarum BM‐04 to synthesize OE, its potential to biotransform EHCO and to survive in extreme environmental conditions. Enzymatic studies of the LDS showed the ability of this fungus to overproduce high amounts of laccase (LACp) or lignin peroxidase (LIPp) in presence of wheat bran or EHCO as sole carbon and energy source, respectively. FT‐IR‐ATR spectroscopy analysis showed the enzymatic oxidation of carbon and sulfur atoms in both maltenes and asphaltenes fractions of biotreated EHCO. Tolerance assays showed the ability of this fungus to grow up to 50000 ppm of EHCO and 2000 mM of NaCl. These results suggest that P. palmarum BM‐04 is a hopeful alternative to be used in remediation processes in extreme environmental conditions of salinity and EHCO‐contamination.</description><identifier>ISSN: 1751-7915</identifier><identifier>EISSN: 1751-7915</identifier><identifier>DOI: 10.1111/1751-7915.12067</identifier><identifier>PMID: 23815379</identifier><language>eng</language><publisher>United States: John Wiley & Sons, Inc</publisher><subject>Analysis ; Biodegradation, Environmental ; Biotransformation ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Fungi ; Industrial Waste - analysis ; Laccase - genetics ; Laccase - metabolism ; Oxidases ; Oxidation-Reduction ; Petroleum - analysis ; Petroleum - metabolism ; Petroleum - microbiology ; Salinity ; Xylariales - enzymology ; Xylariales - genetics ; Xylariales - growth & development ; Xylariales - metabolism</subject><ispartof>Microbial Biotechnology, 2013-11, Vol.6 (6), p.720-730</ispartof><rights>2013 The Authors. published by John Wiley & Sons Ltd and Society for Applied Microbiology.</rights><rights>2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.</rights><rights>COPYRIGHT 2013 John Wiley & Sons, Inc.</rights><rights>Journal compilation © 2013 John Wiley & Sons Ltd and Society for Applied Microbiology 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815938/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815938/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1416,11561,27923,27924,45573,45574,46051,46475,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23815379$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Naranjo‐Briceño, Leopoldo</creatorcontrib><creatorcontrib>Pernía, Beatriz</creatorcontrib><creatorcontrib>Guerra, Mayamaru</creatorcontrib><creatorcontrib>Demey, Jhonny R.</creatorcontrib><creatorcontrib>Sisto, Ángela</creatorcontrib><creatorcontrib>Inojosa, Ysvic</creatorcontrib><creatorcontrib>González, Meralys</creatorcontrib><creatorcontrib>Fusella, Emidio</creatorcontrib><creatorcontrib>Freites, Miguel</creatorcontrib><creatorcontrib>Yegres, Francisco</creatorcontrib><title>Potential role of oxidative exoenzymes of the extremophilic fungus Pestalotiopsis palmarum BM‐04 in biotransformation of extra‐heavy crude oil</title><title>Microbial Biotechnology</title><addtitle>Microb Biotechnol</addtitle><description>Summary
Large amount of drilling waste associated with the expansion of the Orinoco Oil Belt (OOB), the biggest proven reserve of extra‐heavy crude oil (EHCO) worldwide, is usually impregnated with EHCO and highly salinized water‐based drilling fluids. Oxidative exoenzymes (OE) of the lignin‐degrading enzyme system (LDS) of fungi catalyse the oxidation of a wide range of toxic pollutants. However, very little evidences on fungal degradation or biotransformation of EHCO have been reported, which contain high amounts of asphaltenes and its biodegradation rate is very limited. The aims of this work were to study the ability of Pestalotiopsis palmarum BM‐04 to synthesize OE, its potential to biotransform EHCO and to survive in extreme environmental conditions. Enzymatic studies of the LDS showed the ability of this fungus to overproduce high amounts of laccase (LACp) in presence of wheat bran or lignin peroxidase (LIPp) with EHCO as sole carbon and energy source (1300 U mgP−1 in both cases). FT‐IR spectroscopy with Attenuated Total Reflectance (ATR) analysis showed the enzymatic oxidation of carbon and sulfur atoms in both maltenes and asphaltenes fractions of biotreated EHCO catalysed by cell‐free laccase‐enriched OE using wheat bran as inducer. UV‐visible spectrophotometry analysis revealed the oxidation of the petroporphyrins in the asphaltenes fraction of biotreated EHCO. Tolerance assays showed the ability of this fungus to grow up to 50 000 p.p.m. of EHCO and 2000 mM of NaCl. These results suggest that P. palmarum BM‐04 is a hopeful alternative to be used in remediation processes in extreme environmental conditions of salinity and EHCO contamination, such as the drilling waste from the OOB.
Oxidative exoenzymes (OE) of the lignin‐degrading enzyme system (LDS) of fungi catalyze the oxidation of a wide range of toxic pollutants. However, very little evidences on fungal degradation or biotransformation of extra‐heavy crude oil (EHCO) have been reported. The aims of this work were to study the ability of Pestalotiopsis palmarum BM‐04 to synthesize OE, its potential to biotransform EHCO and to survive in extreme environmental conditions. Enzymatic studies of the LDS showed the ability of this fungus to overproduce high amounts of laccase (LACp) or lignin peroxidase (LIPp) in presence of wheat bran or EHCO as sole carbon and energy source, respectively. FT‐IR‐ATR spectroscopy analysis showed the enzymatic oxidation of carbon and sulfur atoms in both maltenes and asphaltenes fractions of biotreated EHCO. Tolerance assays showed the ability of this fungus to grow up to 50000 ppm of EHCO and 2000 mM of NaCl. These results suggest that P. palmarum BM‐04 is a hopeful alternative to be used in remediation processes in extreme environmental conditions of salinity and EHCO‐contamination.</description><subject>Analysis</subject><subject>Biodegradation, Environmental</subject><subject>Biotransformation</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Fungi</subject><subject>Industrial Waste - analysis</subject><subject>Laccase - genetics</subject><subject>Laccase - metabolism</subject><subject>Oxidases</subject><subject>Oxidation-Reduction</subject><subject>Petroleum - analysis</subject><subject>Petroleum - metabolism</subject><subject>Petroleum - microbiology</subject><subject>Salinity</subject><subject>Xylariales - enzymology</subject><subject>Xylariales - genetics</subject><subject>Xylariales - growth & development</subject><subject>Xylariales - metabolism</subject><issn>1751-7915</issn><issn>1751-7915</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNpVUU1P3DAQtRCIr3LmVvkP7GLHmzi-IAHioxKoHOjZmiT2rivHjuJkYTlx4d5DfyG_BJttV2AfPHrz5o1nHkLHlExpPCeU53TCBc2nNCMF30L7G2T7U7yHDkL4TUhBSJ7tor2MlTRnXOyjv_d-UG4wYHHvrcJeY_9kGhjMUmH15JV7XrUqJHxYJGToVeu7hbGmxnp08zHgexUGsH4wvgsm4A5sC_3Yvr28nt-9vfwhM2wcrowfenBB-76N6t4lySQHkbJQsFzhuh-b-ANjv6EdDTaoo3_vIfp1dflwcTO5_Xn94-LsdjJnOeETJgrNKVSgRdFoyqGqMl6UoqQCKCsbQURNIC9qOitq0JpAyTWPjJmoSqWAHaLTtW43Vq1q6riIHqzsehMHWEkPRn7NOLOQc7-UaX2ClVFguhaYg1XSOJ1mrONtVGtq75Q2ET_jjGa8JELEgu-fO25a_TckEvI14TFWrjZ5SmTyWyZHZXJUfvgt784fPgL2Dj6OpKY</recordid><startdate>201311</startdate><enddate>201311</enddate><creator>Naranjo‐Briceño, Leopoldo</creator><creator>Pernía, Beatriz</creator><creator>Guerra, Mayamaru</creator><creator>Demey, Jhonny R.</creator><creator>Sisto, Ángela</creator><creator>Inojosa, Ysvic</creator><creator>González, Meralys</creator><creator>Fusella, Emidio</creator><creator>Freites, Miguel</creator><creator>Yegres, Francisco</creator><general>John Wiley & Sons, Inc</general><general>Blackwell Publishing Ltd</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>IAO</scope><scope>5PM</scope></search><sort><creationdate>201311</creationdate><title>Potential role of oxidative exoenzymes of the extremophilic fungus Pestalotiopsis palmarum BM‐04 in biotransformation of extra‐heavy crude oil</title><author>Naranjo‐Briceño, Leopoldo ; Pernía, Beatriz ; Guerra, Mayamaru ; Demey, Jhonny R. ; Sisto, Ángela ; Inojosa, Ysvic ; González, Meralys ; Fusella, Emidio ; Freites, Miguel ; Yegres, Francisco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g3507-396f71abaf96df17abb27689819a138d909c0a56c146caff0a87f776849b8eea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Analysis</topic><topic>Biodegradation, Environmental</topic><topic>Biotransformation</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>Fungi</topic><topic>Industrial Waste - analysis</topic><topic>Laccase - genetics</topic><topic>Laccase - metabolism</topic><topic>Oxidases</topic><topic>Oxidation-Reduction</topic><topic>Petroleum - analysis</topic><topic>Petroleum - metabolism</topic><topic>Petroleum - microbiology</topic><topic>Salinity</topic><topic>Xylariales - enzymology</topic><topic>Xylariales - genetics</topic><topic>Xylariales - growth & development</topic><topic>Xylariales - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naranjo‐Briceño, Leopoldo</creatorcontrib><creatorcontrib>Pernía, Beatriz</creatorcontrib><creatorcontrib>Guerra, Mayamaru</creatorcontrib><creatorcontrib>Demey, Jhonny R.</creatorcontrib><creatorcontrib>Sisto, Ángela</creatorcontrib><creatorcontrib>Inojosa, Ysvic</creatorcontrib><creatorcontrib>González, Meralys</creatorcontrib><creatorcontrib>Fusella, Emidio</creatorcontrib><creatorcontrib>Freites, Miguel</creatorcontrib><creatorcontrib>Yegres, Francisco</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Free Archive</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Gale Academic OneFile</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Microbial Biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naranjo‐Briceño, Leopoldo</au><au>Pernía, Beatriz</au><au>Guerra, Mayamaru</au><au>Demey, Jhonny R.</au><au>Sisto, Ángela</au><au>Inojosa, Ysvic</au><au>González, Meralys</au><au>Fusella, Emidio</au><au>Freites, Miguel</au><au>Yegres, Francisco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potential role of oxidative exoenzymes of the extremophilic fungus Pestalotiopsis palmarum BM‐04 in biotransformation of extra‐heavy crude oil</atitle><jtitle>Microbial Biotechnology</jtitle><addtitle>Microb Biotechnol</addtitle><date>2013-11</date><risdate>2013</risdate><volume>6</volume><issue>6</issue><spage>720</spage><epage>730</epage><pages>720-730</pages><issn>1751-7915</issn><eissn>1751-7915</eissn><abstract>Summary
Large amount of drilling waste associated with the expansion of the Orinoco Oil Belt (OOB), the biggest proven reserve of extra‐heavy crude oil (EHCO) worldwide, is usually impregnated with EHCO and highly salinized water‐based drilling fluids. Oxidative exoenzymes (OE) of the lignin‐degrading enzyme system (LDS) of fungi catalyse the oxidation of a wide range of toxic pollutants. However, very little evidences on fungal degradation or biotransformation of EHCO have been reported, which contain high amounts of asphaltenes and its biodegradation rate is very limited. The aims of this work were to study the ability of Pestalotiopsis palmarum BM‐04 to synthesize OE, its potential to biotransform EHCO and to survive in extreme environmental conditions. Enzymatic studies of the LDS showed the ability of this fungus to overproduce high amounts of laccase (LACp) in presence of wheat bran or lignin peroxidase (LIPp) with EHCO as sole carbon and energy source (1300 U mgP−1 in both cases). FT‐IR spectroscopy with Attenuated Total Reflectance (ATR) analysis showed the enzymatic oxidation of carbon and sulfur atoms in both maltenes and asphaltenes fractions of biotreated EHCO catalysed by cell‐free laccase‐enriched OE using wheat bran as inducer. UV‐visible spectrophotometry analysis revealed the oxidation of the petroporphyrins in the asphaltenes fraction of biotreated EHCO. Tolerance assays showed the ability of this fungus to grow up to 50 000 p.p.m. of EHCO and 2000 mM of NaCl. These results suggest that P. palmarum BM‐04 is a hopeful alternative to be used in remediation processes in extreme environmental conditions of salinity and EHCO contamination, such as the drilling waste from the OOB.
Oxidative exoenzymes (OE) of the lignin‐degrading enzyme system (LDS) of fungi catalyze the oxidation of a wide range of toxic pollutants. However, very little evidences on fungal degradation or biotransformation of extra‐heavy crude oil (EHCO) have been reported. The aims of this work were to study the ability of Pestalotiopsis palmarum BM‐04 to synthesize OE, its potential to biotransform EHCO and to survive in extreme environmental conditions. Enzymatic studies of the LDS showed the ability of this fungus to overproduce high amounts of laccase (LACp) or lignin peroxidase (LIPp) in presence of wheat bran or EHCO as sole carbon and energy source, respectively. FT‐IR‐ATR spectroscopy analysis showed the enzymatic oxidation of carbon and sulfur atoms in both maltenes and asphaltenes fractions of biotreated EHCO. Tolerance assays showed the ability of this fungus to grow up to 50000 ppm of EHCO and 2000 mM of NaCl. These results suggest that P. palmarum BM‐04 is a hopeful alternative to be used in remediation processes in extreme environmental conditions of salinity and EHCO‐contamination.</abstract><cop>United States</cop><pub>John Wiley & Sons, Inc</pub><pmid>23815379</pmid><doi>10.1111/1751-7915.12067</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Biodegradation, Environmental Biotransformation Fungal Proteins - genetics Fungal Proteins - metabolism Fungi Industrial Waste - analysis Laccase - genetics Laccase - metabolism Oxidases Oxidation-Reduction Petroleum - analysis Petroleum - metabolism Petroleum - microbiology Salinity Xylariales - enzymology Xylariales - genetics Xylariales - growth & development Xylariales - metabolism |
| title | Potential role of oxidative exoenzymes of the extremophilic fungus Pestalotiopsis palmarum BM‐04 in biotransformation of extra‐heavy crude oil |
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