Synthesis of ω‐hydroxy dodecanoic acid based on an engineered CYP153A fusion construct

Summary A bacterial P450 monooxygenase‐based whole cell biocatalyst using Escherichia coli has been applied in the production of ω‐hydroxy dodecanoic acid from dodecanoic acid (C12‐FA) or the corresponding methyl ester. We have constructed and purified a chimeric protein where the fusion of the mono...

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Veröffentlicht in:	Microbial biotechnology 2013-11, Vol.6 (6), p.694-707
Hauptverfasser:	Scheps, Daniel, Honda Malca, Sumire, Richter, Sven M., Marisch, Karoline, Nestl, Bettina M., Hauer, Bernhard
Format:	Artikel
Sprache:	eng
Schlagworte:	Alteromonadaceae - enzymology Alteromonadaceae - genetics Bacillus megaterium - enzymology Bacillus megaterium - genetics Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Escherichia coli - genetics Escherichia coli - metabolism Hydroxylation Lauric Acids - chemistry Lauric Acids - metabolism Metabolic Engineering Protein Engineering Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism
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