The antagonistic strain Bacillus subtilis UMAF6639 also confers protection to melon plants against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses
Summary Biological control of plant diseases has gained acceptance in recent years. Bacillus subtilis UMAF6639 is an antagonistic strain specifically selected for the efficient control of the cucurbit powdery mildew fungus Podosphaera fusca, which is a major threat to cucurbits worldwide. The antago...
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creator | García‐Gutiérrez, Laura Zeriouh, Houda Romero, Diego Cubero, Jaime Vicente, Antonio Pérez‐García, Alejandro |
description | Summary
Biological control of plant diseases has gained acceptance in recent years. Bacillus subtilis UMAF6639 is an antagonistic strain specifically selected for the efficient control of the cucurbit powdery mildew fungus Podosphaera fusca, which is a major threat to cucurbits worldwide. The antagonistic activity relies on the production of the antifungal compounds iturin and fengycin. In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew. In the present work, we further investigated in detail this second mechanism of biocontrol by UMAF6639. First, we examined the signalling pathways elicited by UMAF6639 in melon plants, as well as the defence mechanisms activated in response to P. fusca. Second, we analysed the role of the lipopeptides produced by UMAF6639 as potential determinants for ISR activation. Our results demonstrated that UMAF6639 confers protection against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.
In the present work we investigated in detail the mechanisms by which the antagonistic strain Bacillus subtilis UMAF6639 is also able to induce systemic resistance in melon and provide protection against powdery mildew. Our results demonstrated that UMAF6639 combats disease by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the plant immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent. |
doi_str_mv | 10.1111/1751-7915.12028 |
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Biological control of plant diseases has gained acceptance in recent years. Bacillus subtilis UMAF6639 is an antagonistic strain specifically selected for the efficient control of the cucurbit powdery mildew fungus Podosphaera fusca, which is a major threat to cucurbits worldwide. The antagonistic activity relies on the production of the antifungal compounds iturin and fengycin. In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew. In the present work, we further investigated in detail this second mechanism of biocontrol by UMAF6639. First, we examined the signalling pathways elicited by UMAF6639 in melon plants, as well as the defence mechanisms activated in response to P. fusca. Second, we analysed the role of the lipopeptides produced by UMAF6639 as potential determinants for ISR activation. Our results demonstrated that UMAF6639 confers protection against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.
In the present work we investigated in detail the mechanisms by which the antagonistic strain Bacillus subtilis UMAF6639 is also able to induce systemic resistance in melon and provide protection against powdery mildew. Our results demonstrated that UMAF6639 combats disease by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the plant immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.</description><identifier>ISSN: 1751-7915</identifier><identifier>EISSN: 1751-7915</identifier><identifier>DOI: 10.1111/1751-7915.12028</identifier><identifier>PMID: 23302493</identifier><language>eng</language><publisher>United States: John Wiley & Sons, Inc</publisher><subject>Antibiosis ; Antifungal agents ; Antifungal Agents - metabolism ; Ascomycota - physiology ; Bacillus subtilis - classification ; Bacillus subtilis - metabolism ; Bacillus subtilis - physiology ; Biological control ; Biological Control Agents ; Cucurbitaceae - immunology ; Cucurbitaceae - microbiology ; Cyclopentanes - metabolism ; Fungi ; Lipopeptides - immunology ; Lipopeptides - metabolism ; Oxylipins - metabolism ; Pests ; Phenols ; Plant Diseases - immunology ; Plant Diseases - microbiology ; Plant Leaves - microbiology ; Reactive Oxygen Species - metabolism ; Salicylic Acid - metabolism</subject><ispartof>Microbial Biotechnology, 2013-05, Vol.6 (3), p.264-274</ispartof><rights>2013 The Authors. published by Blackwell Publishing Ltd and Society for Applied Microbiology</rights><rights>2013 The Authors. Published by Society for Applied Microbiology and Blackwell Publishing Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.</rights><rights>COPYRIGHT 2013 John Wiley & Sons, Inc.</rights><rights>2012 The Authors. Published by Society for Applied Microbiology and Blackwell Publishing Ltd. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815921/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815921/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,1418,11564,27926,27927,45576,45577,46054,46478,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23302493$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>García‐Gutiérrez, Laura</creatorcontrib><creatorcontrib>Zeriouh, Houda</creatorcontrib><creatorcontrib>Romero, Diego</creatorcontrib><creatorcontrib>Cubero, Jaime</creatorcontrib><creatorcontrib>Vicente, Antonio</creatorcontrib><creatorcontrib>Pérez‐García, Alejandro</creatorcontrib><title>The antagonistic strain Bacillus subtilis UMAF6639 also confers protection to melon plants against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses</title><title>Microbial Biotechnology</title><addtitle>Microb Biotechnol</addtitle><description>Summary
Biological control of plant diseases has gained acceptance in recent years. Bacillus subtilis UMAF6639 is an antagonistic strain specifically selected for the efficient control of the cucurbit powdery mildew fungus Podosphaera fusca, which is a major threat to cucurbits worldwide. The antagonistic activity relies on the production of the antifungal compounds iturin and fengycin. In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew. In the present work, we further investigated in detail this second mechanism of biocontrol by UMAF6639. First, we examined the signalling pathways elicited by UMAF6639 in melon plants, as well as the defence mechanisms activated in response to P. fusca. Second, we analysed the role of the lipopeptides produced by UMAF6639 as potential determinants for ISR activation. Our results demonstrated that UMAF6639 confers protection against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.
In the present work we investigated in detail the mechanisms by which the antagonistic strain Bacillus subtilis UMAF6639 is also able to induce systemic resistance in melon and provide protection against powdery mildew. Our results demonstrated that UMAF6639 combats disease by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the plant immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.</description><subject>Antibiosis</subject><subject>Antifungal agents</subject><subject>Antifungal Agents - metabolism</subject><subject>Ascomycota - physiology</subject><subject>Bacillus subtilis - classification</subject><subject>Bacillus subtilis - metabolism</subject><subject>Bacillus subtilis - physiology</subject><subject>Biological control</subject><subject>Biological Control Agents</subject><subject>Cucurbitaceae - immunology</subject><subject>Cucurbitaceae - microbiology</subject><subject>Cyclopentanes - metabolism</subject><subject>Fungi</subject><subject>Lipopeptides - immunology</subject><subject>Lipopeptides - metabolism</subject><subject>Oxylipins - metabolism</subject><subject>Pests</subject><subject>Phenols</subject><subject>Plant Diseases - immunology</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Leaves - microbiology</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Salicylic Acid - metabolism</subject><issn>1751-7915</issn><issn>1751-7915</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNpVUk1v1DAQjRCIlsKZG_KRyy7-SLLxBWlbUUBqxWV7thxnkrpy7BA7XeXWC3f-F_-iv4TZ3bIqtiyPZt57fvJMlr1ndMlwfWKrgi1WkhVLximvXmSnx8zLZ_FJ9ibGO0pLSgv-OjvhQlCeS3Ga_dncAtE-6S54G5M1JKZRW0_OtbHOTZHEqU7W2fj48Ovmen1ZlkIS7WIgJvgWxkiGMSQwyQZPUiA9OAwGh5qR6A6lYiJmMtNY20SGsG1gnElvXQNbUs9EI_Ne79mhJXc69sHrBI8Pv9FWQ6J21sx4EGgbzDYwgG_AJ9JAC94AGSEOwUeIb7NXLTqDd0_3WXZz-WVz8W1x9ePr94v11aITBa0WBRVQ0So3rOZ5KQspgWouWKEblsuiZQxrVUmZlJXUpqRaamhly_O24jxn4iz7fNAdprqHxqCZUTs1jLbX46yCtur_ire3qgv3SlSskHwn8PFJYAw_J4hJ9TYacPhpEKaomOD4UkWpQOjyAO20A2V9G1DR4G6gt9gBaC3m1yvB-GrFeYmED8_NHV39azkCigNgi8z5WGdU7SZK7WZG7WZG7SdKXZ9v9oH4CyJKxJ0</recordid><startdate>201305</startdate><enddate>201305</enddate><creator>García‐Gutiérrez, Laura</creator><creator>Zeriouh, Houda</creator><creator>Romero, Diego</creator><creator>Cubero, Jaime</creator><creator>Vicente, Antonio</creator><creator>Pérez‐García, Alejandro</creator><general>John Wiley & Sons, Inc</general><general>Blackwell Publishing Ltd</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>IAO</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201305</creationdate><title>The antagonistic strain Bacillus subtilis UMAF6639 also confers protection to melon plants against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses</title><author>García‐Gutiérrez, Laura ; Zeriouh, Houda ; Romero, Diego ; Cubero, Jaime ; Vicente, Antonio ; Pérez‐García, Alejandro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g3508-503e8084c1b2469599e0a2315ad1495f1184c86019989ac60a9aef9f24f822413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antibiosis</topic><topic>Antifungal agents</topic><topic>Antifungal Agents - metabolism</topic><topic>Ascomycota - physiology</topic><topic>Bacillus subtilis - classification</topic><topic>Bacillus subtilis - metabolism</topic><topic>Bacillus subtilis - physiology</topic><topic>Biological control</topic><topic>Biological Control Agents</topic><topic>Cucurbitaceae - immunology</topic><topic>Cucurbitaceae - microbiology</topic><topic>Cyclopentanes - metabolism</topic><topic>Fungi</topic><topic>Lipopeptides - immunology</topic><topic>Lipopeptides - metabolism</topic><topic>Oxylipins - metabolism</topic><topic>Pests</topic><topic>Phenols</topic><topic>Plant Diseases - immunology</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Leaves - microbiology</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Salicylic Acid - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>García‐Gutiérrez, Laura</creatorcontrib><creatorcontrib>Zeriouh, Houda</creatorcontrib><creatorcontrib>Romero, Diego</creatorcontrib><creatorcontrib>Cubero, Jaime</creatorcontrib><creatorcontrib>Vicente, Antonio</creatorcontrib><creatorcontrib>Pérez‐García, Alejandro</creatorcontrib><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Gale Academic OneFile Select</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Microbial Biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García‐Gutiérrez, Laura</au><au>Zeriouh, Houda</au><au>Romero, Diego</au><au>Cubero, Jaime</au><au>Vicente, Antonio</au><au>Pérez‐García, Alejandro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The antagonistic strain Bacillus subtilis UMAF6639 also confers protection to melon plants against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses</atitle><jtitle>Microbial Biotechnology</jtitle><addtitle>Microb Biotechnol</addtitle><date>2013-05</date><risdate>2013</risdate><volume>6</volume><issue>3</issue><spage>264</spage><epage>274</epage><pages>264-274</pages><issn>1751-7915</issn><eissn>1751-7915</eissn><abstract>Summary
Biological control of plant diseases has gained acceptance in recent years. Bacillus subtilis UMAF6639 is an antagonistic strain specifically selected for the efficient control of the cucurbit powdery mildew fungus Podosphaera fusca, which is a major threat to cucurbits worldwide. The antagonistic activity relies on the production of the antifungal compounds iturin and fengycin. In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew. In the present work, we further investigated in detail this second mechanism of biocontrol by UMAF6639. First, we examined the signalling pathways elicited by UMAF6639 in melon plants, as well as the defence mechanisms activated in response to P. fusca. Second, we analysed the role of the lipopeptides produced by UMAF6639 as potential determinants for ISR activation. Our results demonstrated that UMAF6639 confers protection against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.
In the present work we investigated in detail the mechanisms by which the antagonistic strain Bacillus subtilis UMAF6639 is also able to induce systemic resistance in melon and provide protection against powdery mildew. Our results demonstrated that UMAF6639 combats disease by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the plant immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.</abstract><cop>United States</cop><pub>John Wiley & Sons, Inc</pub><pmid>23302493</pmid><doi>10.1111/1751-7915.12028</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Antibiosis Antifungal agents Antifungal Agents - metabolism Ascomycota - physiology Bacillus subtilis - classification Bacillus subtilis - metabolism Bacillus subtilis - physiology Biological control Biological Control Agents Cucurbitaceae - immunology Cucurbitaceae - microbiology Cyclopentanes - metabolism Fungi Lipopeptides - immunology Lipopeptides - metabolism Oxylipins - metabolism Pests Phenols Plant Diseases - immunology Plant Diseases - microbiology Plant Leaves - microbiology Reactive Oxygen Species - metabolism Salicylic Acid - metabolism |
title | The antagonistic strain Bacillus subtilis UMAF6639 also confers protection to melon plants against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses |
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