The antagonistic strain Bacillus subtilis UMAF6639 also confers protection to melon plants against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses

Summary Biological control of plant diseases has gained acceptance in recent years. Bacillus subtilis UMAF6639 is an antagonistic strain specifically selected for the efficient control of the cucurbit powdery mildew fungus Podosphaera fusca, which is a major threat to cucurbits worldwide. The antago...

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Veröffentlicht in:Microbial Biotechnology 2013-05, Vol.6 (3), p.264-274
Hauptverfasser: García‐Gutiérrez, Laura, Zeriouh, Houda, Romero, Diego, Cubero, Jaime, Vicente, Antonio, Pérez‐García, Alejandro
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container_issue 3
container_start_page 264
container_title Microbial Biotechnology
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creator García‐Gutiérrez, Laura
Zeriouh, Houda
Romero, Diego
Cubero, Jaime
Vicente, Antonio
Pérez‐García, Alejandro
description Summary Biological control of plant diseases has gained acceptance in recent years. Bacillus subtilis UMAF6639 is an antagonistic strain specifically selected for the efficient control of the cucurbit powdery mildew fungus Podosphaera fusca, which is a major threat to cucurbits worldwide. The antagonistic activity relies on the production of the antifungal compounds iturin and fengycin. In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew. In the present work, we further investigated in detail this second mechanism of biocontrol by UMAF6639. First, we examined the signalling pathways elicited by UMAF6639 in melon plants, as well as the defence mechanisms activated in response to P. fusca. Second, we analysed the role of the lipopeptides produced by UMAF6639 as potential determinants for ISR activation. Our results demonstrated that UMAF6639 confers protection against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent. In the present work we investigated in detail the mechanisms by which the antagonistic strain Bacillus subtilis UMAF6639 is also able to induce systemic resistance in melon and provide protection against powdery mildew. Our results demonstrated that UMAF6639 combats disease by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the plant immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.
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Bacillus subtilis UMAF6639 is an antagonistic strain specifically selected for the efficient control of the cucurbit powdery mildew fungus Podosphaera fusca, which is a major threat to cucurbits worldwide. The antagonistic activity relies on the production of the antifungal compounds iturin and fengycin. In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew. In the present work, we further investigated in detail this second mechanism of biocontrol by UMAF6639. First, we examined the signalling pathways elicited by UMAF6639 in melon plants, as well as the defence mechanisms activated in response to P. fusca. Second, we analysed the role of the lipopeptides produced by UMAF6639 as potential determinants for ISR activation. Our results demonstrated that UMAF6639 confers protection against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent. In the present work we investigated in detail the mechanisms by which the antagonistic strain Bacillus subtilis UMAF6639 is also able to induce systemic resistance in melon and provide protection against powdery mildew. Our results demonstrated that UMAF6639 combats disease by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the plant immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.</description><identifier>ISSN: 1751-7915</identifier><identifier>EISSN: 1751-7915</identifier><identifier>DOI: 10.1111/1751-7915.12028</identifier><identifier>PMID: 23302493</identifier><language>eng</language><publisher>United States: John Wiley &amp; Sons, Inc</publisher><subject>Antibiosis ; Antifungal agents ; Antifungal Agents - metabolism ; Ascomycota - physiology ; Bacillus subtilis - classification ; Bacillus subtilis - metabolism ; Bacillus subtilis - physiology ; Biological control ; Biological Control Agents ; Cucurbitaceae - immunology ; Cucurbitaceae - microbiology ; Cyclopentanes - metabolism ; Fungi ; Lipopeptides - immunology ; Lipopeptides - metabolism ; Oxylipins - metabolism ; Pests ; Phenols ; Plant Diseases - immunology ; Plant Diseases - microbiology ; Plant Leaves - microbiology ; Reactive Oxygen Species - metabolism ; Salicylic Acid - metabolism</subject><ispartof>Microbial Biotechnology, 2013-05, Vol.6 (3), p.264-274</ispartof><rights>2013 The Authors. published by Blackwell Publishing Ltd and Society for Applied Microbiology</rights><rights>2013 The Authors. 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Bacillus subtilis UMAF6639 is an antagonistic strain specifically selected for the efficient control of the cucurbit powdery mildew fungus Podosphaera fusca, which is a major threat to cucurbits worldwide. The antagonistic activity relies on the production of the antifungal compounds iturin and fengycin. In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew. In the present work, we further investigated in detail this second mechanism of biocontrol by UMAF6639. First, we examined the signalling pathways elicited by UMAF6639 in melon plants, as well as the defence mechanisms activated in response to P. fusca. Second, we analysed the role of the lipopeptides produced by UMAF6639 as potential determinants for ISR activation. 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We also showed that surfactin lipopeptide is a major determinant for stimulation of the plant immune response. 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Bacillus subtilis UMAF6639 is an antagonistic strain specifically selected for the efficient control of the cucurbit powdery mildew fungus Podosphaera fusca, which is a major threat to cucurbits worldwide. The antagonistic activity relies on the production of the antifungal compounds iturin and fengycin. In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew. In the present work, we further investigated in detail this second mechanism of biocontrol by UMAF6639. First, we examined the signalling pathways elicited by UMAF6639 in melon plants, as well as the defence mechanisms activated in response to P. fusca. Second, we analysed the role of the lipopeptides produced by UMAF6639 as potential determinants for ISR activation. Our results demonstrated that UMAF6639 confers protection against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent. In the present work we investigated in detail the mechanisms by which the antagonistic strain Bacillus subtilis UMAF6639 is also able to induce systemic resistance in melon and provide protection against powdery mildew. Our results demonstrated that UMAF6639 combats disease by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the plant immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.</abstract><cop>United States</cop><pub>John Wiley &amp; Sons, Inc</pub><pmid>23302493</pmid><doi>10.1111/1751-7915.12028</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record>
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subjects Antibiosis
Antifungal agents
Antifungal Agents - metabolism
Ascomycota - physiology
Bacillus subtilis - classification
Bacillus subtilis - metabolism
Bacillus subtilis - physiology
Biological control
Biological Control Agents
Cucurbitaceae - immunology
Cucurbitaceae - microbiology
Cyclopentanes - metabolism
Fungi
Lipopeptides - immunology
Lipopeptides - metabolism
Oxylipins - metabolism
Pests
Phenols
Plant Diseases - immunology
Plant Diseases - microbiology
Plant Leaves - microbiology
Reactive Oxygen Species - metabolism
Salicylic Acid - metabolism
title The antagonistic strain Bacillus subtilis UMAF6639 also confers protection to melon plants against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses
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