A Simple Assay for Measuring Catalase Activity: A Visual Approach
In this study, an assay that combines the ease and simplicity of the qualitative approach for measuring catalase activity was developed. The assay reagents comprised only hydrogen peroxide and Triton X-100. The enzyme-generated oxygen bubbles trapped by Triton X-100 were visualized as foam, whose he...
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| Veröffentlicht in: | Scientific reports 2013-10, Vol.3 (1), p.3081-3081, Article 3081 |
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| creator | Iwase, Tadayuki Tajima, Akiko Sugimoto, Shinya Okuda, Ken-ichi Hironaka, Ippei Kamata, Yuko Takada, Koji Mizunoe, Yoshimitsu |
| description | In this study, an assay that combines the ease and simplicity of the qualitative approach for measuring catalase activity was developed. The assay reagents comprised only hydrogen peroxide and Triton X-100. The enzyme-generated oxygen bubbles trapped by Triton X-100 were visualized as foam, whose height was estimated. A calibration plot using the defined unit of catalase activity yielded the best linear fit over a range of 20–300 units (U) (y = 0.3794x − 2.0909, r
2
= 0.993). The assay precision and reproducibility at 100 U were 4.6% and 4.8%, respectively. The applicability of the assay for measuring the catalase activity of various samples was assessed using laboratory strains of
Escherichia coli
, catalase-deficient isogenic mutants, clinically isolated Shiga toxin-producing
E. coli
, and human cells. The assay generated reproducible results. In conclusion, this new assay can be used to measure the catalase activity of bacterial isolates and human cells. |
| doi_str_mv | 10.1038/srep03081 |
| format | Article |
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2
= 0.993). The assay precision and reproducibility at 100 U were 4.6% and 4.8%, respectively. The applicability of the assay for measuring the catalase activity of various samples was assessed using laboratory strains of
Escherichia coli
, catalase-deficient isogenic mutants, clinically isolated Shiga toxin-producing
E. coli
, and human cells. The assay generated reproducible results. In conclusion, this new assay can be used to measure the catalase activity of bacterial isolates and human cells.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep03081</identifier><identifier>PMID: 24170119</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647/2196/2197 ; 631/326/41/2537 ; Calibration ; Catalase ; Catalase - metabolism ; Cell Line ; E coli ; Enzyme Activation ; Enzyme Assays - methods ; Enzymes ; Escherichia coli ; Genes ; Humanities and Social Sciences ; Humans ; Hydrogen peroxide ; Laboratories ; Medicine ; multidisciplinary ; Mutation ; Oxidative stress ; Pathogens ; Reagents ; Regression analysis ; Reproducibility ; Science ; Science (multidisciplinary) ; Shiga toxin ; Standard deviation ; Toxins</subject><ispartof>Scientific reports, 2013-10, Vol.3 (1), p.3081-3081, Article 3081</ispartof><rights>The Author(s) 2013</rights><rights>Copyright Nature Publishing Group Oct 2013</rights><rights>Copyright © 2013, Macmillan Publishers Limited. All rights reserved 2013 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-6d9d7ebb5b6110fcb402ef8f481b956c3a75dce30f848d2c1ec056030468b2883</citedby><cites>FETCH-LOGICAL-c504t-6d9d7ebb5b6110fcb402ef8f481b956c3a75dce30f848d2c1ec056030468b2883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3812649/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3812649/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24170119$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Iwase, Tadayuki</creatorcontrib><creatorcontrib>Tajima, Akiko</creatorcontrib><creatorcontrib>Sugimoto, Shinya</creatorcontrib><creatorcontrib>Okuda, Ken-ichi</creatorcontrib><creatorcontrib>Hironaka, Ippei</creatorcontrib><creatorcontrib>Kamata, Yuko</creatorcontrib><creatorcontrib>Takada, Koji</creatorcontrib><creatorcontrib>Mizunoe, Yoshimitsu</creatorcontrib><title>A Simple Assay for Measuring Catalase Activity: A Visual Approach</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>In this study, an assay that combines the ease and simplicity of the qualitative approach for measuring catalase activity was developed. The assay reagents comprised only hydrogen peroxide and Triton X-100. The enzyme-generated oxygen bubbles trapped by Triton X-100 were visualized as foam, whose height was estimated. A calibration plot using the defined unit of catalase activity yielded the best linear fit over a range of 20–300 units (U) (y = 0.3794x − 2.0909, r
2
= 0.993). The assay precision and reproducibility at 100 U were 4.6% and 4.8%, respectively. The applicability of the assay for measuring the catalase activity of various samples was assessed using laboratory strains of
Escherichia coli
, catalase-deficient isogenic mutants, clinically isolated Shiga toxin-producing
E. coli
, and human cells. The assay generated reproducible results. In conclusion, this new assay can be used to measure the catalase activity of bacterial isolates and human cells.</description><subject>631/1647/2196/2197</subject><subject>631/326/41/2537</subject><subject>Calibration</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Cell Line</subject><subject>E coli</subject><subject>Enzyme Activation</subject><subject>Enzyme Assays - methods</subject><subject>Enzymes</subject><subject>Escherichia coli</subject><subject>Genes</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Hydrogen peroxide</subject><subject>Laboratories</subject><subject>Medicine</subject><subject>multidisciplinary</subject><subject>Mutation</subject><subject>Oxidative stress</subject><subject>Pathogens</subject><subject>Reagents</subject><subject>Regression analysis</subject><subject>Reproducibility</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Shiga toxin</subject><subject>Standard deviation</subject><subject>Toxins</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNplkVtLwzAUx4Mobsw9-AWk4IsK1dzaJj4IZXiDiQ9eXkOapltGbybtYN_ejM0x9bycA-fH_1z-AJwieI0gYTfO6hYSyNABGGJIoxATjA_36gEYO7eAPiLMKeLHYIApSiBCfAjSNHgzVVvqIHVOroKiscGLlq63pp4FE9nJUjrfVJ1Zmm51G6TBp3G9LIO0bW0j1fwEHBWydHq8zSPw8XD_PnkKp6-Pz5N0GqoI0i6Mc54nOsuiLEYIFiqjEOuCFZShjEexIjKJcqUJLBhlOVZIKxjF_jAaswwzRkbgbqPb9lmlPVp3VpaitaaSdiUaacTvTm3mYtYsBWEIx5R7gYutgG2-eu06URmndFnKWje9E4jShPKEcOLR8z_ooult7c8TiHEGeUxx7KnLDaVs47wNxW4ZBMXaG7HzxrNn-9vvyB8nPHC1AVy7fr22eyP_qX0D4emW0A</recordid><startdate>20131030</startdate><enddate>20131030</enddate><creator>Iwase, Tadayuki</creator><creator>Tajima, Akiko</creator><creator>Sugimoto, Shinya</creator><creator>Okuda, Ken-ichi</creator><creator>Hironaka, Ippei</creator><creator>Kamata, Yuko</creator><creator>Takada, Koji</creator><creator>Mizunoe, Yoshimitsu</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20131030</creationdate><title>A Simple Assay for Measuring Catalase Activity: A Visual Approach</title><author>Iwase, Tadayuki ; 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The assay reagents comprised only hydrogen peroxide and Triton X-100. The enzyme-generated oxygen bubbles trapped by Triton X-100 were visualized as foam, whose height was estimated. A calibration plot using the defined unit of catalase activity yielded the best linear fit over a range of 20–300 units (U) (y = 0.3794x − 2.0909, r
2
= 0.993). The assay precision and reproducibility at 100 U were 4.6% and 4.8%, respectively. The applicability of the assay for measuring the catalase activity of various samples was assessed using laboratory strains of
Escherichia coli
, catalase-deficient isogenic mutants, clinically isolated Shiga toxin-producing
E. coli
, and human cells. The assay generated reproducible results. In conclusion, this new assay can be used to measure the catalase activity of bacterial isolates and human cells.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>24170119</pmid><doi>10.1038/srep03081</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/1647/2196/2197 631/326/41/2537 Calibration Catalase Catalase - metabolism Cell Line E coli Enzyme Activation Enzyme Assays - methods Enzymes Escherichia coli Genes Humanities and Social Sciences Humans Hydrogen peroxide Laboratories Medicine multidisciplinary Mutation Oxidative stress Pathogens Reagents Regression analysis Reproducibility Science Science (multidisciplinary) Shiga toxin Standard deviation Toxins |
| title | A Simple Assay for Measuring Catalase Activity: A Visual Approach |
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