Super-resolution fluorescence imaging with single molecules

•Molecular specificity and live-cell compatibility are key fluorescence strengths.•Time-sequential imaging and precise localization of sparse single-molecule emitters yield super-resolution.•Advances in 3D multicolor and fast time-lapse are highlighted.•Dipole-emission-induced position errors and mo...

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Veröffentlicht in:	Current opinion in structural biology 2013-10, Vol.23 (5), p.778-787
Hauptverfasser:	Sahl, Steffen J, Moerner, WE
Format:	Artikel
Sprache:	eng
Schlagworte:	Imaging, Three-Dimensional - methods Microscopy, Fluorescence - methods Molecular Imaging - methods Multiprotein Complexes - chemistry Organelles - chemistry Organelles - ultrastructure Time-Lapse Imaging - methods
Online-Zugang:	Volltext
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