Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin
In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhim...
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Veröffentlicht in: | Microbiology (Society for General Microbiology) 2013-10, Vol.159 (Pt 10), p.2049-2057 |
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creator | Guerrero, P Collao, B Álvarez, R Salinas, H Morales, E H Calderón, I L Saavedra, C P Gil, F |
description | In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhimurium) participates in the detection of ciprofloxacin, a bactericidal antibiotic, and in the positive regulation of mdtA, an efflux pump implicated in antibiotic resistance. In the present work, we provide further evidence for a role of the S. Typhimurium BaeSR two-component system in response to ciprofloxacin treatment and show that it regulates sodA expression. We demonstrate that, in the absence of BaeSR, the transcript levels of sodA and the activity of its gene product are lower. Using electrophoretic mobility shift assays and transcriptional fusions, we demonstrate that BaeR regulates sodA by a direct interaction with the promoter region. |
doi_str_mv | 10.1099/mic.0.066787-0 |
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We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhimurium) participates in the detection of ciprofloxacin, a bactericidal antibiotic, and in the positive regulation of mdtA, an efflux pump implicated in antibiotic resistance. In the present work, we provide further evidence for a role of the S. Typhimurium BaeSR two-component system in response to ciprofloxacin treatment and show that it regulates sodA expression. We demonstrate that, in the absence of BaeSR, the transcript levels of sodA and the activity of its gene product are lower. Using electrophoretic mobility shift assays and transcriptional fusions, we demonstrate that BaeR regulates sodA by a direct interaction with the promoter region.</description><identifier>ISSN: 1350-0872</identifier><identifier>EISSN: 1465-2080</identifier><identifier>DOI: 10.1099/mic.0.066787-0</identifier><identifier>PMID: 23918818</identifier><language>eng</language><publisher>England: Society for General Microbiology</publisher><subject>Anti-Bacterial Agents - metabolism ; Artificial Gene Fusion ; Bacterial Proteins - biosynthesis ; Cell and Molecular Biology of Microbes ; Ciprofloxacin - metabolism ; DNA, Bacterial - metabolism ; Electrophoretic Mobility Shift Assay ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; Gene Knockout Techniques ; Multidrug Resistance-Associated Proteins - genetics ; Multidrug Resistance-Associated Proteins - metabolism ; Promoter Regions, Genetic ; Protein Binding ; Protein Kinases - genetics ; Protein Kinases - metabolism ; Salmonella typhimurium - drug effects ; Superoxide Dismutase - biosynthesis ; Trans-Activators - genetics ; Trans-Activators - metabolism ; Transcription, Genetic</subject><ispartof>Microbiology (Society for General Microbiology), 2013-10, Vol.159 (Pt 10), p.2049-2057</ispartof><rights>2013 SGM</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-751c6fe702148a6da130af528aeb78bb11a270568db69b197a0370c0ef56b4aa3</citedby><cites>FETCH-LOGICAL-c390t-751c6fe702148a6da130af528aeb78bb11a270568db69b197a0370c0ef56b4aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799227/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3799227/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23918818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guerrero, P</creatorcontrib><creatorcontrib>Collao, B</creatorcontrib><creatorcontrib>Álvarez, R</creatorcontrib><creatorcontrib>Salinas, H</creatorcontrib><creatorcontrib>Morales, E H</creatorcontrib><creatorcontrib>Calderón, I L</creatorcontrib><creatorcontrib>Saavedra, C P</creatorcontrib><creatorcontrib>Gil, F</creatorcontrib><title>Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhimurium) participates in the detection of ciprofloxacin, a bactericidal antibiotic, and in the positive regulation of mdtA, an efflux pump implicated in antibiotic resistance. In the present work, we provide further evidence for a role of the S. Typhimurium BaeSR two-component system in response to ciprofloxacin treatment and show that it regulates sodA expression. We demonstrate that, in the absence of BaeSR, the transcript levels of sodA and the activity of its gene product are lower. Using electrophoretic mobility shift assays and transcriptional fusions, we demonstrate that BaeR regulates sodA by a direct interaction with the promoter region.</description><subject>Anti-Bacterial Agents - metabolism</subject><subject>Artificial Gene Fusion</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Cell and Molecular Biology of Microbes</subject><subject>Ciprofloxacin - metabolism</subject><subject>DNA, Bacterial - metabolism</subject><subject>Electrophoretic Mobility Shift Assay</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene Knockout Techniques</subject><subject>Multidrug Resistance-Associated Proteins - genetics</subject><subject>Multidrug Resistance-Associated Proteins - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Binding</subject><subject>Protein Kinases - genetics</subject><subject>Protein Kinases - metabolism</subject><subject>Salmonella typhimurium - drug effects</subject><subject>Superoxide Dismutase - biosynthesis</subject><subject>Trans-Activators - genetics</subject><subject>Trans-Activators - metabolism</subject><subject>Transcription, Genetic</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUUtv1DAQthCIlsKVI_KRS5axndjOBalUvKRKSLScrYnXaY3iONjO0vx7XG2p4DSj-R4zmo-Q1wx2DPr-XfB2BzuQUmnVwBNyylrZNRw0PK296KABrfgJeZHzT4AKAntOTrjomdZMn5LtCqcQZzdNSN1cXPIWaXYpHjDR62259WFNfg30A7qr77T8jo2NYamKudC85eICXWL2xR_ctNHkbtYJi8s0x_059XOd5MrOjpZIrV9SHKd4h9bPL8mzEafsXj3UM_Lj08friy_N5bfPXy_OLxsreiiN6piVo1PAWatR7pEJwLHjGt2g9DAwhlxBJ_V-kP3AeoUgFFhwYyeHFlGckfdH32UdgtvbenjCySzJB0ybiejN_8jsb81NPBih-p5zVQ3ePhik-Gt1uZjgs73_2Ozimg1rWyE0452s1N2RalPMObnxcQ0Dc59XlVoD5piXgSp48-9xj_S_AYk_3YKV0Q</recordid><startdate>201310</startdate><enddate>201310</enddate><creator>Guerrero, P</creator><creator>Collao, B</creator><creator>Álvarez, R</creator><creator>Salinas, H</creator><creator>Morales, E H</creator><creator>Calderón, I L</creator><creator>Saavedra, C P</creator><creator>Gil, F</creator><general>Society for General Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201310</creationdate><title>Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin</title><author>Guerrero, P ; Collao, B ; Álvarez, R ; Salinas, H ; Morales, E H ; Calderón, I L ; Saavedra, C P ; Gil, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-751c6fe702148a6da130af528aeb78bb11a270568db69b197a0370c0ef56b4aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Anti-Bacterial Agents - metabolism</topic><topic>Artificial Gene Fusion</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Cell and Molecular Biology of Microbes</topic><topic>Ciprofloxacin - metabolism</topic><topic>DNA, Bacterial - metabolism</topic><topic>Electrophoretic Mobility Shift Assay</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene Knockout Techniques</topic><topic>Multidrug Resistance-Associated Proteins - genetics</topic><topic>Multidrug Resistance-Associated Proteins - metabolism</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Binding</topic><topic>Protein Kinases - genetics</topic><topic>Protein Kinases - metabolism</topic><topic>Salmonella typhimurium - drug effects</topic><topic>Superoxide Dismutase - biosynthesis</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guerrero, P</creatorcontrib><creatorcontrib>Collao, B</creatorcontrib><creatorcontrib>Álvarez, R</creatorcontrib><creatorcontrib>Salinas, H</creatorcontrib><creatorcontrib>Morales, E H</creatorcontrib><creatorcontrib>Calderón, I L</creatorcontrib><creatorcontrib>Saavedra, C P</creatorcontrib><creatorcontrib>Gil, F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guerrero, P</au><au>Collao, B</au><au>Álvarez, R</au><au>Salinas, H</au><au>Morales, E H</au><au>Calderón, I L</au><au>Saavedra, C P</au><au>Gil, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2013-10</date><risdate>2013</risdate><volume>159</volume><issue>Pt 10</issue><spage>2049</spage><epage>2057</epage><pages>2049-2057</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhimurium) participates in the detection of ciprofloxacin, a bactericidal antibiotic, and in the positive regulation of mdtA, an efflux pump implicated in antibiotic resistance. In the present work, we provide further evidence for a role of the S. Typhimurium BaeSR two-component system in response to ciprofloxacin treatment and show that it regulates sodA expression. We demonstrate that, in the absence of BaeSR, the transcript levels of sodA and the activity of its gene product are lower. Using electrophoretic mobility shift assays and transcriptional fusions, we demonstrate that BaeR regulates sodA by a direct interaction with the promoter region.</abstract><cop>England</cop><pub>Society for General Microbiology</pub><pmid>23918818</pmid><doi>10.1099/mic.0.066787-0</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Anti-Bacterial Agents - metabolism Artificial Gene Fusion Bacterial Proteins - biosynthesis Cell and Molecular Biology of Microbes Ciprofloxacin - metabolism DNA, Bacterial - metabolism Electrophoretic Mobility Shift Assay Gene Expression Profiling Gene Expression Regulation, Bacterial Gene Knockout Techniques Multidrug Resistance-Associated Proteins - genetics Multidrug Resistance-Associated Proteins - metabolism Promoter Regions, Genetic Protein Binding Protein Kinases - genetics Protein Kinases - metabolism Salmonella typhimurium - drug effects Superoxide Dismutase - biosynthesis Trans-Activators - genetics Trans-Activators - metabolism Transcription, Genetic |
title | Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin |
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