Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin

In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhim...

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Veröffentlicht in:Microbiology (Society for General Microbiology) 2013-10, Vol.159 (Pt 10), p.2049-2057
Hauptverfasser: Guerrero, P, Collao, B, Álvarez, R, Salinas, H, Morales, E H, Calderón, I L, Saavedra, C P, Gil, F
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container_end_page 2057
container_issue Pt 10
container_start_page 2049
container_title Microbiology (Society for General Microbiology)
container_volume 159
creator Guerrero, P
Collao, B
Álvarez, R
Salinas, H
Morales, E H
Calderón, I L
Saavedra, C P
Gil, F
description In response to antibiotics, bacteria activate regulatory systems that control the expression of genes that participate in detoxifying these compounds, like multidrug efflux systems. We previously demonstrated that the BaeSR two-component system from Salmonella enterica serovar Typhimurium (S. Typhimurium) participates in the detection of ciprofloxacin, a bactericidal antibiotic, and in the positive regulation of mdtA, an efflux pump implicated in antibiotic resistance. In the present work, we provide further evidence for a role of the S. Typhimurium BaeSR two-component system in response to ciprofloxacin treatment and show that it regulates sodA expression. We demonstrate that, in the absence of BaeSR, the transcript levels of sodA and the activity of its gene product are lower. Using electrophoretic mobility shift assays and transcriptional fusions, we demonstrate that BaeR regulates sodA by a direct interaction with the promoter region.
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subjects Anti-Bacterial Agents - metabolism
Artificial Gene Fusion
Bacterial Proteins - biosynthesis
Cell and Molecular Biology of Microbes
Ciprofloxacin - metabolism
DNA, Bacterial - metabolism
Electrophoretic Mobility Shift Assay
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Gene Knockout Techniques
Multidrug Resistance-Associated Proteins - genetics
Multidrug Resistance-Associated Proteins - metabolism
Promoter Regions, Genetic
Protein Binding
Protein Kinases - genetics
Protein Kinases - metabolism
Salmonella typhimurium - drug effects
Superoxide Dismutase - biosynthesis
Trans-Activators - genetics
Trans-Activators - metabolism
Transcription, Genetic
title Salmonella enterica serovar Typhimurium BaeSR two-component system positively regulates sodA in response to ciprofloxacin
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