Dissecting non-coding RNA mechanisms in cellulo by Single-molecule High-Resolution Localization and Counting

•Method to probe microRNAs in cellulo with single-molecule sensitivity.•Microinjection and HILO microscopy to deliver and image fluorophore labeled miRNAs.•Single particle tracking in live cells to obtain diffusion coefficients.•Fixed-cell single molecule counting by stepwise photobleaching for stoi...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2013-09, Vol.63 (2), p.188-199
Hauptverfasser:	Pitchiaya, Sethuramasundaram, Krishnan, Vishalakshi, Custer, Thomas C., Walter, Nils G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence diffusivity Fluorescent Dyes - chemistry Genes, Reporter HeLa Cells Humans Luciferases, Firefly - biosynthesis Luciferases, Firefly - genetics Luciferases, Renilla - biosynthesis Luciferases, Renilla - genetics mammals mechanism of action Microinjections microRNA MicroRNAs - chemistry MicroRNAs - genetics MicroRNAs - metabolism Microscopy, Fluorescence Non-coding RNA regulator genes Ribonucleoproteins - metabolism RNA Interference RNA silencing Single molecule microscopy Single particle tracking Single-Cell Analysis - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!