Nanoscale-Targeted Patch-Clamp Recordings of Functional Presynaptic Ion Channels
Direct electrical access to presynaptic ion channels has hitherto been limited to large specialized terminals such as the calyx of Held or hippocampal mossy fiber bouton. The electrophysiology and ion-channel complement of far more abundant small synaptic terminals (≤1 μm) remain poorly understood....
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| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 2013-09, Vol.79 (6), p.1067-1077 |
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| creator | Novak, Pavel Gorelik, Julia Vivekananda, Umesh Shevchuk, Andrew I. Ermolyuk, Yaroslav S. Bailey, Russell J. Bushby, Andrew J. Moss, Guy W.J. Rusakov, Dmitri A. Klenerman, David Kullmann, Dimitri M. Volynski, Kirill E. Korchev, Yuri E. |
| description | Direct electrical access to presynaptic ion channels has hitherto been limited to large specialized terminals such as the calyx of Held or hippocampal mossy fiber bouton. The electrophysiology and ion-channel complement of far more abundant small synaptic terminals (≤1 μm) remain poorly understood. Here we report a method based on superresolution scanning ion conductance imaging of small synapses in culture at approximately 100–150 nm 3D resolution, which allows presynaptic patch-clamp recordings in all four configurations (cell-attached, inside-out, outside-out, and whole-cell). Using this technique, we report presynaptic recordings of K+, Na+, Cl−, and Ca2+ channels. This semiautomated approach allows direct investigation of the distribution and properties of presynaptic ion channels at small central synapses.
[Display omitted]
•Topographic imaging of live synaptic boutons at nanoscale resolution•Cell-attached patch-clamp recordings of ion channels in small central synapses•Whole-cell small presynaptic bouton recordings
Novak and colleagues have developed a method for nanoscale-targeted patch-clamp presynaptic recordings in submicrometer central synapses identified using superresolution scanning ion conductance microscopy. This semiautomated approach opens a new window on the physiology of small presynaptic terminals. |
| doi_str_mv | 10.1016/j.neuron.2013.07.012 |
| format | Article |
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[Display omitted]
•Topographic imaging of live synaptic boutons at nanoscale resolution•Cell-attached patch-clamp recordings of ion channels in small central synapses•Whole-cell small presynaptic bouton recordings
Novak and colleagues have developed a method for nanoscale-targeted patch-clamp presynaptic recordings in submicrometer central synapses identified using superresolution scanning ion conductance microscopy. This semiautomated approach opens a new window on the physiology of small presynaptic terminals.</description><identifier>ISSN: 0896-6273</identifier><identifier>EISSN: 1097-4199</identifier><identifier>DOI: 10.1016/j.neuron.2013.07.012</identifier><identifier>PMID: 24050398</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Animals, Newborn ; Biophysical Phenomena - physiology ; Calcium - metabolism ; Cells, Cultured ; Dendritic Spines - physiology ; Dendritic Spines - ultrastructure ; Electric Stimulation ; Electrodes ; Experiments ; Fluorescent Dyes - metabolism ; Hippocampus - cytology ; Imaging, Three-Dimensional ; Ion Channel Gating - physiology ; Ion Channels - physiology ; Ion Channels - ultrastructure ; Membrane Potentials - physiology ; Microscopy ; Microscopy, Scanning Tunneling ; Neurons - cytology ; NeuroResource ; Patch-Clamp Techniques ; Presynaptic Terminals - physiology ; Presynaptic Terminals - ultrastructure ; Rats</subject><ispartof>Neuron (Cambridge, Mass.), 2013-09, Vol.79 (6), p.1067-1077</ispartof><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. All rights reserved.</rights><rights>Copyright Elsevier Limited Sep 18, 2013</rights><rights>2013 ELL & Excerpta Medica. 2013 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c627t-9a05e9bdc17175fc16bcb61dd6dad294985de9f3696f4ae6820a6633b5bff86b3</citedby><cites>FETCH-LOGICAL-c627t-9a05e9bdc17175fc16bcb61dd6dad294985de9f3696f4ae6820a6633b5bff86b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0896627313006120$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24050398$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Novak, Pavel</creatorcontrib><creatorcontrib>Gorelik, Julia</creatorcontrib><creatorcontrib>Vivekananda, Umesh</creatorcontrib><creatorcontrib>Shevchuk, Andrew I.</creatorcontrib><creatorcontrib>Ermolyuk, Yaroslav S.</creatorcontrib><creatorcontrib>Bailey, Russell J.</creatorcontrib><creatorcontrib>Bushby, Andrew J.</creatorcontrib><creatorcontrib>Moss, Guy W.J.</creatorcontrib><creatorcontrib>Rusakov, Dmitri A.</creatorcontrib><creatorcontrib>Klenerman, David</creatorcontrib><creatorcontrib>Kullmann, Dimitri M.</creatorcontrib><creatorcontrib>Volynski, Kirill E.</creatorcontrib><creatorcontrib>Korchev, Yuri E.</creatorcontrib><title>Nanoscale-Targeted Patch-Clamp Recordings of Functional Presynaptic Ion Channels</title><title>Neuron (Cambridge, Mass.)</title><addtitle>Neuron</addtitle><description>Direct electrical access to presynaptic ion channels has hitherto been limited to large specialized terminals such as the calyx of Held or hippocampal mossy fiber bouton. The electrophysiology and ion-channel complement of far more abundant small synaptic terminals (≤1 μm) remain poorly understood. Here we report a method based on superresolution scanning ion conductance imaging of small synapses in culture at approximately 100–150 nm 3D resolution, which allows presynaptic patch-clamp recordings in all four configurations (cell-attached, inside-out, outside-out, and whole-cell). Using this technique, we report presynaptic recordings of K+, Na+, Cl−, and Ca2+ channels. This semiautomated approach allows direct investigation of the distribution and properties of presynaptic ion channels at small central synapses.
[Display omitted]
•Topographic imaging of live synaptic boutons at nanoscale resolution•Cell-attached patch-clamp recordings of ion channels in small central synapses•Whole-cell small presynaptic bouton recordings
Novak and colleagues have developed a method for nanoscale-targeted patch-clamp presynaptic recordings in submicrometer central synapses identified using superresolution scanning ion conductance microscopy. This semiautomated approach opens a new window on the physiology of small presynaptic terminals.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biophysical Phenomena - physiology</subject><subject>Calcium - metabolism</subject><subject>Cells, Cultured</subject><subject>Dendritic Spines - physiology</subject><subject>Dendritic Spines - ultrastructure</subject><subject>Electric Stimulation</subject><subject>Electrodes</subject><subject>Experiments</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Hippocampus - cytology</subject><subject>Imaging, Three-Dimensional</subject><subject>Ion Channel Gating - physiology</subject><subject>Ion Channels - physiology</subject><subject>Ion Channels - ultrastructure</subject><subject>Membrane Potentials - physiology</subject><subject>Microscopy</subject><subject>Microscopy, Scanning Tunneling</subject><subject>Neurons - cytology</subject><subject>NeuroResource</subject><subject>Patch-Clamp Techniques</subject><subject>Presynaptic Terminals - physiology</subject><subject>Presynaptic Terminals - ultrastructure</subject><subject>Rats</subject><issn>0896-6273</issn><issn>1097-4199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EokvhHyAUiQuXBH87viChVQuVKlihcrYce7LrVdZe7KRS_z2utpSPA6c5zDPvzLwvQq8J7ggm8v2-i7DkFDuKCeuw6jChT9CKYK1aTrR-ila417KVVLEz9KKUPcaEC02eozPKscBM9yu0-WJjKs5O0N7YvIUZfLOxs9u168kejs03cCn7ELelSWNzuUQ3hxTt1GwylLtoj3NwzVWKzXpnY4SpvETPRjsVePVQz9H3y4ub9ef2-uunq_XH69bVg-ZWWyxAD94RRZQYHZGDGyTxXnrrqea6Fx70yKSWI7cge4qtlIwNYhjHXg7sHH046R6X4QDeQZyzncwxh4PNdybZYP7uxLAz23RrmOoJo7IKvHsQyOnHAmU2h1AcTJONkJZiCGdccYqFqOjbf9B9WnJ1oVLVx15Irmil-IlyOZWSYXw8hmBzH5nZm1Nk5j4yg5WpkdWxN38-8jj0K6Pfn1Z34TZANsUFiA58yOBm41P4_4afo4Oq3Q</recordid><startdate>20130918</startdate><enddate>20130918</enddate><creator>Novak, Pavel</creator><creator>Gorelik, Julia</creator><creator>Vivekananda, Umesh</creator><creator>Shevchuk, Andrew I.</creator><creator>Ermolyuk, Yaroslav S.</creator><creator>Bailey, Russell J.</creator><creator>Bushby, Andrew J.</creator><creator>Moss, Guy W.J.</creator><creator>Rusakov, Dmitri A.</creator><creator>Klenerman, David</creator><creator>Kullmann, Dimitri M.</creator><creator>Volynski, Kirill E.</creator><creator>Korchev, Yuri E.</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><general>Cell Press</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130918</creationdate><title>Nanoscale-Targeted Patch-Clamp Recordings of Functional Presynaptic Ion Channels</title><author>Novak, Pavel ; 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The electrophysiology and ion-channel complement of far more abundant small synaptic terminals (≤1 μm) remain poorly understood. Here we report a method based on superresolution scanning ion conductance imaging of small synapses in culture at approximately 100–150 nm 3D resolution, which allows presynaptic patch-clamp recordings in all four configurations (cell-attached, inside-out, outside-out, and whole-cell). Using this technique, we report presynaptic recordings of K+, Na+, Cl−, and Ca2+ channels. This semiautomated approach allows direct investigation of the distribution and properties of presynaptic ion channels at small central synapses.
[Display omitted]
•Topographic imaging of live synaptic boutons at nanoscale resolution•Cell-attached patch-clamp recordings of ion channels in small central synapses•Whole-cell small presynaptic bouton recordings
Novak and colleagues have developed a method for nanoscale-targeted patch-clamp presynaptic recordings in submicrometer central synapses identified using superresolution scanning ion conductance microscopy. This semiautomated approach opens a new window on the physiology of small presynaptic terminals.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>24050398</pmid><doi>10.1016/j.neuron.2013.07.012</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Neuron (Cambridge, Mass.), 2013-09, Vol.79 (6), p.1067-1077 |
| issn | 0896-6273 1097-4199 |
| language | eng |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Animals, Newborn Biophysical Phenomena - physiology Calcium - metabolism Cells, Cultured Dendritic Spines - physiology Dendritic Spines - ultrastructure Electric Stimulation Electrodes Experiments Fluorescent Dyes - metabolism Hippocampus - cytology Imaging, Three-Dimensional Ion Channel Gating - physiology Ion Channels - physiology Ion Channels - ultrastructure Membrane Potentials - physiology Microscopy Microscopy, Scanning Tunneling Neurons - cytology NeuroResource Patch-Clamp Techniques Presynaptic Terminals - physiology Presynaptic Terminals - ultrastructure Rats |
| title | Nanoscale-Targeted Patch-Clamp Recordings of Functional Presynaptic Ion Channels |
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