Measuring the mechanical properties of blood clots formed via the tissue factor pathway of coagulation
Thrombelastography (TEG) is a method that is used to conduct global assays that monitor fibrin formation and fibrinolysis and platelet aggregation in whole blood. The purpose of this study was to use a well-characterized tissue factor (Tf) reagent and contact pathway inhibitor (corn trypsin inhibito...
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| Veröffentlicht in: | Analytical biochemistry 2012-03, Vol.422 (1), p.46-51 |
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| creator | Foley, J.H. Butenas, S. Mann, K.G. Brummel-Ziedins, K.E. |
| description | Thrombelastography (TEG) is a method that is used to conduct global assays that monitor fibrin formation and fibrinolysis and platelet aggregation in whole blood. The purpose of this study was to use a well-characterized tissue factor (Tf) reagent and contact pathway inhibitor (corn trypsin inhibitor, CTI) to develop a reproducible thrombelastography assay. In this study, blood was collected from 5 male subjects (three times). Clot formation was initiated in whole blood with 5pM Tf in the presence of CTI, and fibrinolysis was induced by adding tissue plasminogen activator (tPA). Changes in viscoelasticity were then monitored by TEG. In quality control assays, our Tf reagent, when used at 5pM, induced coagulation in whole blood in 3.93±0.23min and in plasma in 5.12±0.23min (n=3). In TEG assays, tPA significantly decreased clot strength (maximum amplitude, MA) in all individuals but had no effect on clot time (R time). The intraassay variability (CVa |
| doi_str_mv | 10.1016/j.ab.2011.12.036 |
| format | Article |
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The purpose of this study was to use a well-characterized tissue factor (Tf) reagent and contact pathway inhibitor (corn trypsin inhibitor, CTI) to develop a reproducible thrombelastography assay. In this study, blood was collected from 5 male subjects (three times). Clot formation was initiated in whole blood with 5pM Tf in the presence of CTI, and fibrinolysis was induced by adding tissue plasminogen activator (tPA). Changes in viscoelasticity were then monitored by TEG. In quality control assays, our Tf reagent, when used at 5pM, induced coagulation in whole blood in 3.93±0.23min and in plasma in 5.12±0.23min (n=3). In TEG assays, tPA significantly decreased clot strength (maximum amplitude, MA) in all individuals but had no effect on clot time (R time). The intraassay variability (CVa<10%) for R time, angle, and MA suggests that these parameters reliably describe the dynamics of fibrin formation and degradation in whole blood. Our Tf reagent reproducibly induces coagulation, making it an ideal tool to quantify the processes that contribute to mechanical clot strength in whole blood.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2011.12.036</identifier><identifier>PMID: 22266209</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; blood ; Blood Cell Count ; Blood Coagulation ; Blood Coagulation Tests - methods ; Blood Viscosity ; coagulation ; corn ; degradation ; dynamics ; Elasticity ; Fibrin ; Fibrin - chemistry ; Fibrinolysis ; Humans ; Male ; males ; mechanical properties ; PAI-1 ; Plant Proteins - chemistry ; plasminogen activator ; Platelet Activation ; platelet aggregation ; Quality Control ; Reproducibility of Results ; t-plasminogen activator ; Thrombelastography ; Thrombelastography - methods ; Thromboplastin - chemistry ; Time Factors ; tPA ; trypsin inhibitors ; viscoelasticity</subject><ispartof>Analytical biochemistry, 2012-03, Vol.422 (1), p.46-51</ispartof><rights>2012 Elsevier Inc.</rights><rights>Copyright © 2012 Elsevier Inc. All rights reserved.</rights><rights>2012 Elsevier Inc. All rights reserved. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-fc93bcfb83c4a1a453cda4367de8dc2c65ea5039a9b2aef53ac45b256b02a62f3</citedby><cites>FETCH-LOGICAL-c470t-fc93bcfb83c4a1a453cda4367de8dc2c65ea5039a9b2aef53ac45b256b02a62f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ab.2011.12.036$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,782,786,887,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22266209$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Foley, J.H.</creatorcontrib><creatorcontrib>Butenas, S.</creatorcontrib><creatorcontrib>Mann, K.G.</creatorcontrib><creatorcontrib>Brummel-Ziedins, K.E.</creatorcontrib><title>Measuring the mechanical properties of blood clots formed via the tissue factor pathway of coagulation</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Thrombelastography (TEG) is a method that is used to conduct global assays that monitor fibrin formation and fibrinolysis and platelet aggregation in whole blood. The purpose of this study was to use a well-characterized tissue factor (Tf) reagent and contact pathway inhibitor (corn trypsin inhibitor, CTI) to develop a reproducible thrombelastography assay. In this study, blood was collected from 5 male subjects (three times). Clot formation was initiated in whole blood with 5pM Tf in the presence of CTI, and fibrinolysis was induced by adding tissue plasminogen activator (tPA). Changes in viscoelasticity were then monitored by TEG. In quality control assays, our Tf reagent, when used at 5pM, induced coagulation in whole blood in 3.93±0.23min and in plasma in 5.12±0.23min (n=3). In TEG assays, tPA significantly decreased clot strength (maximum amplitude, MA) in all individuals but had no effect on clot time (R time). The intraassay variability (CVa<10%) for R time, angle, and MA suggests that these parameters reliably describe the dynamics of fibrin formation and degradation in whole blood. Our Tf reagent reproducibly induces coagulation, making it an ideal tool to quantify the processes that contribute to mechanical clot strength in whole blood.</description><subject>Adult</subject><subject>blood</subject><subject>Blood Cell Count</subject><subject>Blood Coagulation</subject><subject>Blood Coagulation Tests - methods</subject><subject>Blood Viscosity</subject><subject>coagulation</subject><subject>corn</subject><subject>degradation</subject><subject>dynamics</subject><subject>Elasticity</subject><subject>Fibrin</subject><subject>Fibrin - chemistry</subject><subject>Fibrinolysis</subject><subject>Humans</subject><subject>Male</subject><subject>males</subject><subject>mechanical properties</subject><subject>PAI-1</subject><subject>Plant Proteins - chemistry</subject><subject>plasminogen activator</subject><subject>Platelet Activation</subject><subject>platelet aggregation</subject><subject>Quality Control</subject><subject>Reproducibility of Results</subject><subject>t-plasminogen activator</subject><subject>Thrombelastography</subject><subject>Thrombelastography - methods</subject><subject>Thromboplastin - chemistry</subject><subject>Time Factors</subject><subject>tPA</subject><subject>trypsin inhibitors</subject><subject>viscoelasticity</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kT2P1DAQhi0E4paDngrcUSX4I3E2FEjoxJd0iAKutiaT8a5X2XixnUX37_GyxwkKKhd-3mdG8zL2XIpaCmle72oYaiWkrKWqhTYP2EqK3lRCi_4hWwkhdKVM312wJyntRAGb1jxmF0opY5ToV8x9IUhL9POG5y3xPeEWZo8w8UMMB4rZU-LB8WEKYeQ4hZy4C3FPIz96-J3JPqWFuAPMIfID5O1PuD1lMMBmmSD7MD9ljxxMiZ7dvZfs5sP771efquuvHz9fvbuusOlErhz2ekA3rDU2IKFpNY7QaNONtB5RoWkJWqF76AcF5FoN2LSDas0gFBjl9CV7e_YelqHsiDTnCJM9RL-HeGsDePvvz-y3dhOOVnfd2pimCF7dCWL4sVDKdu8T0jTBTGFJtleyNV05ciHFmcQYUork7qdIYU_t2J2FwZ7asVLZ0k6JvPh7u_vAnzoK8PIMOAgWNtEne_OtGExpUq6lOSnenAkqVzx6ijahpxlp9JEw2zH4_8__BZn8q7A</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Foley, J.H.</creator><creator>Butenas, S.</creator><creator>Mann, K.G.</creator><creator>Brummel-Ziedins, K.E.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120301</creationdate><title>Measuring the mechanical properties of blood clots formed via the tissue factor pathway of coagulation</title><author>Foley, J.H. ; Butenas, S. ; Mann, K.G. ; Brummel-Ziedins, K.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-fc93bcfb83c4a1a453cda4367de8dc2c65ea5039a9b2aef53ac45b256b02a62f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>blood</topic><topic>Blood Cell Count</topic><topic>Blood Coagulation</topic><topic>Blood Coagulation Tests - methods</topic><topic>Blood Viscosity</topic><topic>coagulation</topic><topic>corn</topic><topic>degradation</topic><topic>dynamics</topic><topic>Elasticity</topic><topic>Fibrin</topic><topic>Fibrin - chemistry</topic><topic>Fibrinolysis</topic><topic>Humans</topic><topic>Male</topic><topic>males</topic><topic>mechanical properties</topic><topic>PAI-1</topic><topic>Plant Proteins - chemistry</topic><topic>plasminogen activator</topic><topic>Platelet Activation</topic><topic>platelet aggregation</topic><topic>Quality Control</topic><topic>Reproducibility of Results</topic><topic>t-plasminogen activator</topic><topic>Thrombelastography</topic><topic>Thrombelastography - methods</topic><topic>Thromboplastin - chemistry</topic><topic>Time Factors</topic><topic>tPA</topic><topic>trypsin inhibitors</topic><topic>viscoelasticity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Foley, J.H.</creatorcontrib><creatorcontrib>Butenas, S.</creatorcontrib><creatorcontrib>Mann, K.G.</creatorcontrib><creatorcontrib>Brummel-Ziedins, K.E.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Foley, J.H.</au><au>Butenas, S.</au><au>Mann, K.G.</au><au>Brummel-Ziedins, K.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measuring the mechanical properties of blood clots formed via the tissue factor pathway of coagulation</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>422</volume><issue>1</issue><spage>46</spage><epage>51</epage><pages>46-51</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Thrombelastography (TEG) is a method that is used to conduct global assays that monitor fibrin formation and fibrinolysis and platelet aggregation in whole blood. The purpose of this study was to use a well-characterized tissue factor (Tf) reagent and contact pathway inhibitor (corn trypsin inhibitor, CTI) to develop a reproducible thrombelastography assay. In this study, blood was collected from 5 male subjects (three times). Clot formation was initiated in whole blood with 5pM Tf in the presence of CTI, and fibrinolysis was induced by adding tissue plasminogen activator (tPA). Changes in viscoelasticity were then monitored by TEG. In quality control assays, our Tf reagent, when used at 5pM, induced coagulation in whole blood in 3.93±0.23min and in plasma in 5.12±0.23min (n=3). In TEG assays, tPA significantly decreased clot strength (maximum amplitude, MA) in all individuals but had no effect on clot time (R time). The intraassay variability (CVa<10%) for R time, angle, and MA suggests that these parameters reliably describe the dynamics of fibrin formation and degradation in whole blood. Our Tf reagent reproducibly induces coagulation, making it an ideal tool to quantify the processes that contribute to mechanical clot strength in whole blood.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>22266209</pmid><doi>10.1016/j.ab.2011.12.036</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult blood Blood Cell Count Blood Coagulation Blood Coagulation Tests - methods Blood Viscosity coagulation corn degradation dynamics Elasticity Fibrin Fibrin - chemistry Fibrinolysis Humans Male males mechanical properties PAI-1 Plant Proteins - chemistry plasminogen activator Platelet Activation platelet aggregation Quality Control Reproducibility of Results t-plasminogen activator Thrombelastography Thrombelastography - methods Thromboplastin - chemistry Time Factors tPA trypsin inhibitors viscoelasticity |
| title | Measuring the mechanical properties of blood clots formed via the tissue factor pathway of coagulation |
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