Measuring the mechanical properties of blood clots formed via the tissue factor pathway of coagulation

Thrombelastography (TEG) is a method that is used to conduct global assays that monitor fibrin formation and fibrinolysis and platelet aggregation in whole blood. The purpose of this study was to use a well-characterized tissue factor (Tf) reagent and contact pathway inhibitor (corn trypsin inhibito...

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Veröffentlicht in:Analytical biochemistry 2012-03, Vol.422 (1), p.46-51
Hauptverfasser: Foley, J.H., Butenas, S., Mann, K.G., Brummel-Ziedins, K.E.
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container_issue 1
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container_title Analytical biochemistry
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creator Foley, J.H.
Butenas, S.
Mann, K.G.
Brummel-Ziedins, K.E.
description Thrombelastography (TEG) is a method that is used to conduct global assays that monitor fibrin formation and fibrinolysis and platelet aggregation in whole blood. The purpose of this study was to use a well-characterized tissue factor (Tf) reagent and contact pathway inhibitor (corn trypsin inhibitor, CTI) to develop a reproducible thrombelastography assay. In this study, blood was collected from 5 male subjects (three times). Clot formation was initiated in whole blood with 5pM Tf in the presence of CTI, and fibrinolysis was induced by adding tissue plasminogen activator (tPA). Changes in viscoelasticity were then monitored by TEG. In quality control assays, our Tf reagent, when used at 5pM, induced coagulation in whole blood in 3.93±0.23min and in plasma in 5.12±0.23min (n=3). In TEG assays, tPA significantly decreased clot strength (maximum amplitude, MA) in all individuals but had no effect on clot time (R time). The intraassay variability (CVa
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The purpose of this study was to use a well-characterized tissue factor (Tf) reagent and contact pathway inhibitor (corn trypsin inhibitor, CTI) to develop a reproducible thrombelastography assay. In this study, blood was collected from 5 male subjects (three times). Clot formation was initiated in whole blood with 5pM Tf in the presence of CTI, and fibrinolysis was induced by adding tissue plasminogen activator (tPA). Changes in viscoelasticity were then monitored by TEG. In quality control assays, our Tf reagent, when used at 5pM, induced coagulation in whole blood in 3.93±0.23min and in plasma in 5.12±0.23min (n=3). In TEG assays, tPA significantly decreased clot strength (maximum amplitude, MA) in all individuals but had no effect on clot time (R time). The intraassay variability (CVa&lt;10%) for R time, angle, and MA suggests that these parameters reliably describe the dynamics of fibrin formation and degradation in whole blood. Our Tf reagent reproducibly induces coagulation, making it an ideal tool to quantify the processes that contribute to mechanical clot strength in whole blood.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/j.ab.2011.12.036</identifier><identifier>PMID: 22266209</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; blood ; Blood Cell Count ; Blood Coagulation ; Blood Coagulation Tests - methods ; Blood Viscosity ; coagulation ; corn ; degradation ; dynamics ; Elasticity ; Fibrin ; Fibrin - chemistry ; Fibrinolysis ; Humans ; Male ; males ; mechanical properties ; PAI-1 ; Plant Proteins - chemistry ; plasminogen activator ; Platelet Activation ; platelet aggregation ; Quality Control ; Reproducibility of Results ; t-plasminogen activator ; Thrombelastography ; Thrombelastography - methods ; Thromboplastin - chemistry ; Time Factors ; tPA ; trypsin inhibitors ; viscoelasticity</subject><ispartof>Analytical biochemistry, 2012-03, Vol.422 (1), p.46-51</ispartof><rights>2012 Elsevier Inc.</rights><rights>Copyright © 2012 Elsevier Inc. 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All rights reserved. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-fc93bcfb83c4a1a453cda4367de8dc2c65ea5039a9b2aef53ac45b256b02a62f3</citedby><cites>FETCH-LOGICAL-c470t-fc93bcfb83c4a1a453cda4367de8dc2c65ea5039a9b2aef53ac45b256b02a62f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ab.2011.12.036$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,782,786,887,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22266209$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Foley, J.H.</creatorcontrib><creatorcontrib>Butenas, S.</creatorcontrib><creatorcontrib>Mann, K.G.</creatorcontrib><creatorcontrib>Brummel-Ziedins, K.E.</creatorcontrib><title>Measuring the mechanical properties of blood clots formed via the tissue factor pathway of coagulation</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Thrombelastography (TEG) is a method that is used to conduct global assays that monitor fibrin formation and fibrinolysis and platelet aggregation in whole blood. The purpose of this study was to use a well-characterized tissue factor (Tf) reagent and contact pathway inhibitor (corn trypsin inhibitor, CTI) to develop a reproducible thrombelastography assay. In this study, blood was collected from 5 male subjects (three times). Clot formation was initiated in whole blood with 5pM Tf in the presence of CTI, and fibrinolysis was induced by adding tissue plasminogen activator (tPA). Changes in viscoelasticity were then monitored by TEG. In quality control assays, our Tf reagent, when used at 5pM, induced coagulation in whole blood in 3.93±0.23min and in plasma in 5.12±0.23min (n=3). In TEG assays, tPA significantly decreased clot strength (maximum amplitude, MA) in all individuals but had no effect on clot time (R time). The intraassay variability (CVa&lt;10%) for R time, angle, and MA suggests that these parameters reliably describe the dynamics of fibrin formation and degradation in whole blood. Our Tf reagent reproducibly induces coagulation, making it an ideal tool to quantify the processes that contribute to mechanical clot strength in whole blood.</description><subject>Adult</subject><subject>blood</subject><subject>Blood Cell Count</subject><subject>Blood Coagulation</subject><subject>Blood Coagulation Tests - methods</subject><subject>Blood Viscosity</subject><subject>coagulation</subject><subject>corn</subject><subject>degradation</subject><subject>dynamics</subject><subject>Elasticity</subject><subject>Fibrin</subject><subject>Fibrin - chemistry</subject><subject>Fibrinolysis</subject><subject>Humans</subject><subject>Male</subject><subject>males</subject><subject>mechanical properties</subject><subject>PAI-1</subject><subject>Plant Proteins - chemistry</subject><subject>plasminogen activator</subject><subject>Platelet Activation</subject><subject>platelet aggregation</subject><subject>Quality Control</subject><subject>Reproducibility of Results</subject><subject>t-plasminogen activator</subject><subject>Thrombelastography</subject><subject>Thrombelastography - methods</subject><subject>Thromboplastin - chemistry</subject><subject>Time Factors</subject><subject>tPA</subject><subject>trypsin inhibitors</subject><subject>viscoelasticity</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kT2P1DAQhi0E4paDngrcUSX4I3E2FEjoxJd0iAKutiaT8a5X2XixnUX37_GyxwkKKhd-3mdG8zL2XIpaCmle72oYaiWkrKWqhTYP2EqK3lRCi_4hWwkhdKVM312wJyntRAGb1jxmF0opY5ToV8x9IUhL9POG5y3xPeEWZo8w8UMMB4rZU-LB8WEKYeQ4hZy4C3FPIz96-J3JPqWFuAPMIfID5O1PuD1lMMBmmSD7MD9ljxxMiZ7dvZfs5sP771efquuvHz9fvbuusOlErhz2ekA3rDU2IKFpNY7QaNONtB5RoWkJWqF76AcF5FoN2LSDas0gFBjl9CV7e_YelqHsiDTnCJM9RL-HeGsDePvvz-y3dhOOVnfd2pimCF7dCWL4sVDKdu8T0jTBTGFJtleyNV05ciHFmcQYUork7qdIYU_t2J2FwZ7asVLZ0k6JvPh7u_vAnzoK8PIMOAgWNtEne_OtGExpUq6lOSnenAkqVzx6ijahpxlp9JEw2zH4_8__BZn8q7A</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Foley, J.H.</creator><creator>Butenas, S.</creator><creator>Mann, K.G.</creator><creator>Brummel-Ziedins, K.E.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120301</creationdate><title>Measuring the mechanical properties of blood clots formed via the tissue factor pathway of coagulation</title><author>Foley, J.H. ; Butenas, S. ; Mann, K.G. ; Brummel-Ziedins, K.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-fc93bcfb83c4a1a453cda4367de8dc2c65ea5039a9b2aef53ac45b256b02a62f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>blood</topic><topic>Blood Cell Count</topic><topic>Blood Coagulation</topic><topic>Blood Coagulation Tests - methods</topic><topic>Blood Viscosity</topic><topic>coagulation</topic><topic>corn</topic><topic>degradation</topic><topic>dynamics</topic><topic>Elasticity</topic><topic>Fibrin</topic><topic>Fibrin - chemistry</topic><topic>Fibrinolysis</topic><topic>Humans</topic><topic>Male</topic><topic>males</topic><topic>mechanical properties</topic><topic>PAI-1</topic><topic>Plant Proteins - chemistry</topic><topic>plasminogen activator</topic><topic>Platelet Activation</topic><topic>platelet aggregation</topic><topic>Quality Control</topic><topic>Reproducibility of Results</topic><topic>t-plasminogen activator</topic><topic>Thrombelastography</topic><topic>Thrombelastography - methods</topic><topic>Thromboplastin - chemistry</topic><topic>Time Factors</topic><topic>tPA</topic><topic>trypsin inhibitors</topic><topic>viscoelasticity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Foley, J.H.</creatorcontrib><creatorcontrib>Butenas, S.</creatorcontrib><creatorcontrib>Mann, K.G.</creatorcontrib><creatorcontrib>Brummel-Ziedins, K.E.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Foley, J.H.</au><au>Butenas, S.</au><au>Mann, K.G.</au><au>Brummel-Ziedins, K.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measuring the mechanical properties of blood clots formed via the tissue factor pathway of coagulation</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>422</volume><issue>1</issue><spage>46</spage><epage>51</epage><pages>46-51</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Thrombelastography (TEG) is a method that is used to conduct global assays that monitor fibrin formation and fibrinolysis and platelet aggregation in whole blood. 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subjects Adult
blood
Blood Cell Count
Blood Coagulation
Blood Coagulation Tests - methods
Blood Viscosity
coagulation
corn
degradation
dynamics
Elasticity
Fibrin
Fibrin - chemistry
Fibrinolysis
Humans
Male
males
mechanical properties
PAI-1
Plant Proteins - chemistry
plasminogen activator
Platelet Activation
platelet aggregation
Quality Control
Reproducibility of Results
t-plasminogen activator
Thrombelastography
Thrombelastography - methods
Thromboplastin - chemistry
Time Factors
tPA
trypsin inhibitors
viscoelasticity
title Measuring the mechanical properties of blood clots formed via the tissue factor pathway of coagulation
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