Antioxidant, Antiproliferative, and Antiangiogenesis Effects of Polyphenol-Rich Seaweed (Sargassum muticum)
In the present study, we evaluated the effect of brown seaweeds Sargassum muticum methanolic extract (SMME), against MCF-7 and MDA-MB-231 breast cancer cell lines proliferation. This algae extract was also evaluated for reducing activity and total polyphenol content. The MTT assay results indicated...
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| creator | Fargahi, Fahimeh Zafar-Balanejad, Saeedeh Baharara, Javad Mohammad, Rosfarizan Namvar, Farideh Rahman, Heshu Sulaiman |
| description | In the present study, we evaluated the effect of brown seaweeds Sargassum muticum methanolic extract (SMME), against MCF-7 and MDA-MB-231 breast cancer cell lines proliferation. This algae extract was also evaluated for reducing activity and total polyphenol content. The MTT assay results indicated that the extracts were cytotoxic against breast cancer cell lines in a dose-dependent manner, with IC50 of 22 μg/ml for MCF-7 and 55 μg/ml for MDA-MB-231 cell lines. The percentages of apoptotic MCF-7-treated cells increased from 13% to 67% by increasing the concentration of the SMME. The antiproliferative efficacy of this algal extract was positively correlated with the total polyphenol contents, suggesting a causal link related to extract content of phenolic acids. Cell cycle analysis showed a significant increase in the accumulation of SMME-treated cells at sub-G1 phase, indicating the induction of apoptosis by SMME. Further apoptosis induction was confirmed by Hoechst 33342 and AO/PI staining. Also SMME implanted in vivo into fertilized chicken eggs induced dose-related antiangiogenic activity in the chorioallantoic membrane (CAM). Our results imply a new insight on the novel function of Sargassum muticum polyphenol-rich seaweed in cancer research by induction of apoptosis, antioxidant, and antiangiogenesis effects. |
| doi_str_mv | 10.1155/2013/604787 |
| format | Article |
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This algae extract was also evaluated for reducing activity and total polyphenol content. The MTT assay results indicated that the extracts were cytotoxic against breast cancer cell lines in a dose-dependent manner, with IC50 of 22 μg/ml for MCF-7 and 55 μg/ml for MDA-MB-231 cell lines. The percentages of apoptotic MCF-7-treated cells increased from 13% to 67% by increasing the concentration of the SMME. The antiproliferative efficacy of this algal extract was positively correlated with the total polyphenol contents, suggesting a causal link related to extract content of phenolic acids. Cell cycle analysis showed a significant increase in the accumulation of SMME-treated cells at sub-G1 phase, indicating the induction of apoptosis by SMME. Further apoptosis induction was confirmed by Hoechst 33342 and AO/PI staining. Also SMME implanted in vivo into fertilized chicken eggs induced dose-related antiangiogenic activity in the chorioallantoic membrane (CAM). Our results imply a new insight on the novel function of Sargassum muticum polyphenol-rich seaweed in cancer research by induction of apoptosis, antioxidant, and antiangiogenesis effects.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2013/604787</identifier><identifier>PMID: 24078922</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Algae ; Analysis ; Angiogenesis ; Angiogenesis Inhibitors - pharmacology ; Animals ; Antioxidants ; Antioxidants - pharmacology ; Apoptosis ; Biology ; Breast cancer ; Breast Neoplasms - blood supply ; Breast Neoplasms - enzymology ; Breast Neoplasms - pathology ; Breast Neoplasms - ultrastructure ; Caspases - metabolism ; Cell culture ; Cell Cycle - drug effects ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cell Shape - drug effects ; Chickens ; Chlorocebus aethiops ; Cytotoxicity ; Female ; Flow Cytometry ; Functional foods ; Functional foods & nutraceuticals ; Health aspects ; Humans ; Marine algae as food ; Neovascularization, Pathologic - drug therapy ; Neovascularization, Pathologic - pathology ; Nutritional aspects ; Oxidative stress ; Phytotherapy ; Polyphenols ; Polyphenols - pharmacology ; Polyphenols - therapeutic use ; Rodents ; Sargassum - chemistry ; Sargassum muticum ; Seaweed - chemistry ; Studies ; Vero Cells</subject><ispartof>BioMed research international, 2013-01, Vol.2013 (2013), p.1-9</ispartof><rights>Copyright © 2013 Farideh Namvar et al.</rights><rights>COPYRIGHT 2013 John Wiley & Sons, Inc.</rights><rights>Copyright © 2013 Farideh Namvar et al. Farideh Namvar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2013 Farideh Namvar et al. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c594t-d2ca6bdf4554824f3ca8692f3f6327efa84900443734d9dbc2956050b9580f843</citedby><cites>FETCH-LOGICAL-c594t-d2ca6bdf4554824f3ca8692f3f6327efa84900443734d9dbc2956050b9580f843</cites><orcidid>0000-0002-9097-7880 ; 0000-0001-8490-0230 ; 0000-0003-0356-0399</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776361/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3776361/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,27907,27908,53774,53776</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24078922$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Anneè, Jozef</contributor><creatorcontrib>Fargahi, Fahimeh</creatorcontrib><creatorcontrib>Zafar-Balanejad, Saeedeh</creatorcontrib><creatorcontrib>Baharara, Javad</creatorcontrib><creatorcontrib>Mohammad, Rosfarizan</creatorcontrib><creatorcontrib>Namvar, Farideh</creatorcontrib><creatorcontrib>Rahman, Heshu Sulaiman</creatorcontrib><title>Antioxidant, Antiproliferative, and Antiangiogenesis Effects of Polyphenol-Rich Seaweed (Sargassum muticum)</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>In the present study, we evaluated the effect of brown seaweeds Sargassum muticum methanolic extract (SMME), against MCF-7 and MDA-MB-231 breast cancer cell lines proliferation. This algae extract was also evaluated for reducing activity and total polyphenol content. The MTT assay results indicated that the extracts were cytotoxic against breast cancer cell lines in a dose-dependent manner, with IC50 of 22 μg/ml for MCF-7 and 55 μg/ml for MDA-MB-231 cell lines. The percentages of apoptotic MCF-7-treated cells increased from 13% to 67% by increasing the concentration of the SMME. The antiproliferative efficacy of this algal extract was positively correlated with the total polyphenol contents, suggesting a causal link related to extract content of phenolic acids. Cell cycle analysis showed a significant increase in the accumulation of SMME-treated cells at sub-G1 phase, indicating the induction of apoptosis by SMME. Further apoptosis induction was confirmed by Hoechst 33342 and AO/PI staining. Also SMME implanted in vivo into fertilized chicken eggs induced dose-related antiangiogenic activity in the chorioallantoic membrane (CAM). Our results imply a new insight on the novel function of Sargassum muticum polyphenol-rich seaweed in cancer research by induction of apoptosis, antioxidant, and antiangiogenesis effects.</description><subject>Algae</subject><subject>Analysis</subject><subject>Angiogenesis</subject><subject>Angiogenesis Inhibitors - pharmacology</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Apoptosis</subject><subject>Biology</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - blood supply</subject><subject>Breast Neoplasms - enzymology</subject><subject>Breast Neoplasms - pathology</subject><subject>Breast Neoplasms - ultrastructure</subject><subject>Caspases - metabolism</subject><subject>Cell culture</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Shape - drug effects</subject><subject>Chickens</subject><subject>Chlorocebus aethiops</subject><subject>Cytotoxicity</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Functional foods</subject><subject>Functional foods & nutraceuticals</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Marine algae as food</subject><subject>Neovascularization, Pathologic - drug therapy</subject><subject>Neovascularization, Pathologic - pathology</subject><subject>Nutritional aspects</subject><subject>Oxidative stress</subject><subject>Phytotherapy</subject><subject>Polyphenols</subject><subject>Polyphenols - pharmacology</subject><subject>Polyphenols - therapeutic use</subject><subject>Rodents</subject><subject>Sargassum - chemistry</subject><subject>Sargassum muticum</subject><subject>Seaweed - chemistry</subject><subject>Studies</subject><subject>Vero Cells</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkl1PFDEUhhujEbJy5b2ZxBsURvrd6Y3JhoCSkGhEr5tup50tzrRLOwPy7-mysKJXNCfp15O3Pee8ALxF8BNCjB1hiMgRh1Q04gXYxQTRmiOKXm7XhOyAvZwvYRkN4lDy12AHUygaifEu-D0Po49_fKvDeFitN6sUe-9s0qO_toeVDu39sQ6dj50NNvtcnThnzZir6Krvsb9dLW2Iff3Dm2V1YfWNtW21f6FTp3OehmqYRm-m4cMb8MrpPtu9h3kGfp2e_Dz-Wp9_-3J2PD-vDZN0rFtsNF-0jjJGG0wdMbrhEjviOMHCOt1QCSGlRBDaynZhsGQcMriQrIGuoWQGPm90V9NisK2xYUy6V6vkB51uVdRe_XsT_FJ18VoRITgpJZuB_QeBFK8mm0c1-Gxs3-tg45QVKo9TWII-CyUCUUYK-v4_9DJOKZRKFIoIKhuG-V-q071VPrhYvmjWompeMi5R2l6ogw1lUsw5WbfNDkG1NoZaG0NtjFHod08LsmUfbVCAjxtg6UOrb_zz1GxBSjuewAQKKMkdj5nH-w</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Fargahi, Fahimeh</creator><creator>Zafar-Balanejad, Saeedeh</creator><creator>Baharara, Javad</creator><creator>Mohammad, Rosfarizan</creator><creator>Namvar, Farideh</creator><creator>Rahman, Heshu Sulaiman</creator><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9097-7880</orcidid><orcidid>https://orcid.org/0000-0001-8490-0230</orcidid><orcidid>https://orcid.org/0000-0003-0356-0399</orcidid></search><sort><creationdate>20130101</creationdate><title>Antioxidant, Antiproliferative, and Antiangiogenesis Effects of Polyphenol-Rich Seaweed (Sargassum muticum)</title><author>Fargahi, Fahimeh ; Zafar-Balanejad, Saeedeh ; Baharara, Javad ; Mohammad, Rosfarizan ; Namvar, Farideh ; Rahman, Heshu Sulaiman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c594t-d2ca6bdf4554824f3ca8692f3f6327efa84900443734d9dbc2956050b9580f843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Algae</topic><topic>Analysis</topic><topic>Angiogenesis</topic><topic>Angiogenesis Inhibitors - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fargahi, Fahimeh</au><au>Zafar-Balanejad, Saeedeh</au><au>Baharara, Javad</au><au>Mohammad, Rosfarizan</au><au>Namvar, Farideh</au><au>Rahman, Heshu Sulaiman</au><au>Anneè, Jozef</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant, Antiproliferative, and Antiangiogenesis Effects of Polyphenol-Rich Seaweed (Sargassum muticum)</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>2013</volume><issue>2013</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>In the present study, we evaluated the effect of brown seaweeds Sargassum muticum methanolic extract (SMME), against MCF-7 and MDA-MB-231 breast cancer cell lines proliferation. This algae extract was also evaluated for reducing activity and total polyphenol content. The MTT assay results indicated that the extracts were cytotoxic against breast cancer cell lines in a dose-dependent manner, with IC50 of 22 μg/ml for MCF-7 and 55 μg/ml for MDA-MB-231 cell lines. The percentages of apoptotic MCF-7-treated cells increased from 13% to 67% by increasing the concentration of the SMME. The antiproliferative efficacy of this algal extract was positively correlated with the total polyphenol contents, suggesting a causal link related to extract content of phenolic acids. Cell cycle analysis showed a significant increase in the accumulation of SMME-treated cells at sub-G1 phase, indicating the induction of apoptosis by SMME. Further apoptosis induction was confirmed by Hoechst 33342 and AO/PI staining. Also SMME implanted in vivo into fertilized chicken eggs induced dose-related antiangiogenic activity in the chorioallantoic membrane (CAM). Our results imply a new insight on the novel function of Sargassum muticum polyphenol-rich seaweed in cancer research by induction of apoptosis, antioxidant, and antiangiogenesis effects.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>24078922</pmid><doi>10.1155/2013/604787</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9097-7880</orcidid><orcidid>https://orcid.org/0000-0001-8490-0230</orcidid><orcidid>https://orcid.org/0000-0003-0356-0399</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Algae Analysis Angiogenesis Angiogenesis Inhibitors - pharmacology Animals Antioxidants Antioxidants - pharmacology Apoptosis Biology Breast cancer Breast Neoplasms - blood supply Breast Neoplasms - enzymology Breast Neoplasms - pathology Breast Neoplasms - ultrastructure Caspases - metabolism Cell culture Cell Cycle - drug effects Cell Line, Tumor Cell Proliferation - drug effects Cell Shape - drug effects Chickens Chlorocebus aethiops Cytotoxicity Female Flow Cytometry Functional foods Functional foods & nutraceuticals Health aspects Humans Marine algae as food Neovascularization, Pathologic - drug therapy Neovascularization, Pathologic - pathology Nutritional aspects Oxidative stress Phytotherapy Polyphenols Polyphenols - pharmacology Polyphenols - therapeutic use Rodents Sargassum - chemistry Sargassum muticum Seaweed - chemistry Studies Vero Cells |
| title | Antioxidant, Antiproliferative, and Antiangiogenesis Effects of Polyphenol-Rich Seaweed (Sargassum muticum) |
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