Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling
We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model of MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach of combining DESI-MSI wit...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2013-05, Vol.85 (9), p.4259-4262 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Perry, Richard H Bellovin, David I Shroff, Emelyn H Ismail, Ali I Zabuawala, Tahera Felsher, Dean W Zare, Richard N |
| description | We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model of MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach of combining DESI-MSI with the ability to turn specific genes on and off has led to the discovery of highly specific lipid molecules associated with MYC-induced tumor onset. We are able to distinguish normal from MYC-induced malignant cells. Our approach provides a strategy to define a precise molecular picture at a resolution of about 200 μm that may be useful in identifying lipid molecules that define how the MYC oncogene initiates and maintains tumorigenesis. |
| doi_str_mv | 10.1021/ac400479j |
| format | Article |
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This unique, label-free approach of combining DESI-MSI with the ability to turn specific genes on and off has led to the discovery of highly specific lipid molecules associated with MYC-induced tumor onset. We are able to distinguish normal from MYC-induced malignant cells. Our approach provides a strategy to define a precise molecular picture at a resolution of about 200 μm that may be useful in identifying lipid molecules that define how the MYC oncogene initiates and maintains tumorigenesis.</description><identifier>ISSN: 0003-2700</identifier><identifier>ISSN: 1520-6882</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac400479j</identifier><identifier>PMID: 23560736</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Analytical chemistry ; animal models ; Animals ; carcinogenesis ; Carcinogenesis - genetics ; Carcinogenesis - metabolism ; Carcinoma, Hepatocellular - genetics ; Carcinoma, Hepatocellular - metabolism ; Carcinoma, Hepatocellular - pathology ; desorption ; Disease Models, Animal ; electrospray ionization mass spectrometry ; hepatoma ; image analysis ; lipid composition ; Lipid Metabolism - genetics ; Lipids ; Lipids - genetics ; Liver cancer ; Liver Neoplasms - genetics ; Liver Neoplasms - metabolism ; Liver Neoplasms - pathology ; Mass spectrometry ; Mice ; Mice, Transgenic ; Molecules ; oncogenes ; Proto-Oncogene Proteins c-myc - genetics ; Proto-Oncogene Proteins c-myc - metabolism ; Spectrometry, Mass, Electrospray Ionization ; tissues ; Transgenic animals</subject><ispartof>Analytical chemistry (Washington), 2013-05, Vol.85 (9), p.4259-4262</ispartof><rights>Copyright © 2013 American Chemical Society</rights><rights>Copyright American Chemical Society May 7, 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a466t-6339d34c9adf9677ca1d40ac3cee5307b31997fd6c8e882490c788bec7d822d13</citedby><cites>FETCH-LOGICAL-a466t-6339d34c9adf9677ca1d40ac3cee5307b31997fd6c8e882490c788bec7d822d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac400479j$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac400479j$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,776,780,881,2751,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23560736$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Perry, Richard H</creatorcontrib><creatorcontrib>Bellovin, David I</creatorcontrib><creatorcontrib>Shroff, Emelyn H</creatorcontrib><creatorcontrib>Ismail, Ali I</creatorcontrib><creatorcontrib>Zabuawala, Tahera</creatorcontrib><creatorcontrib>Felsher, Dean W</creatorcontrib><creatorcontrib>Zare, Richard N</creatorcontrib><title>Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model of MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach of combining DESI-MSI with the ability to turn specific genes on and off has led to the discovery of highly specific lipid molecules associated with MYC-induced tumor onset. We are able to distinguish normal from MYC-induced malignant cells. Our approach provides a strategy to define a precise molecular picture at a resolution of about 200 μm that may be useful in identifying lipid molecules that define how the MYC oncogene initiates and maintains tumorigenesis.</description><subject>Analytical chemistry</subject><subject>animal models</subject><subject>Animals</subject><subject>carcinogenesis</subject><subject>Carcinogenesis - genetics</subject><subject>Carcinogenesis - metabolism</subject><subject>Carcinoma, Hepatocellular - genetics</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>desorption</subject><subject>Disease Models, Animal</subject><subject>electrospray ionization mass spectrometry</subject><subject>hepatoma</subject><subject>image analysis</subject><subject>lipid composition</subject><subject>Lipid Metabolism - genetics</subject><subject>Lipids</subject><subject>Lipids - genetics</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - genetics</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - pathology</subject><subject>Mass spectrometry</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Molecules</subject><subject>oncogenes</subject><subject>Proto-Oncogene Proteins c-myc - genetics</subject><subject>Proto-Oncogene Proteins c-myc - metabolism</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>tissues</subject><subject>Transgenic animals</subject><issn>0003-2700</issn><issn>1520-6882</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkc1qGzEURkVoiN2ki75AGSiBZDHJlTQjjTaFYtI64JBA00VXQpY0jsxYcqSZgPP0VXBi3HR1F_dw7s-H0GcMFxgIvlS6Aqi4WB6gMa4JlKxpyAc0BgBaEg4wQh9TWgJgDJgdoRGhNQNO2RhNJw8qKt3b6J5V74IvQlvc_JmU194M2prifliF6BbW2-RSMd8Uzhe_XD8UM7d2priLoXWd84sTdNiqLtlPr_UY_f5xdT-ZlrPbn9eT77NSVYz1JaNUGFppoUwrGOdaYVOB0lRbW1Pgc4qF4K1hurH5iEqA5k0zt5qbhhCD6TH6tvWuh_nKGm19H1Un19GtVNzIoJz8t-Pdg1yEJ0k5p5VgWXD2KojhcbCplyuXtO065W0YkiT5a4Q1wOqMfn2HLsMQfT5P4uzigtYNz9T5ltIxpBRtu1sGg3zJR-7yyeyX_e135FsgGTjdAkqnvWn_if4C5QCWYw</recordid><startdate>20130507</startdate><enddate>20130507</enddate><creator>Perry, Richard H</creator><creator>Bellovin, David I</creator><creator>Shroff, Emelyn H</creator><creator>Ismail, Ali I</creator><creator>Zabuawala, Tahera</creator><creator>Felsher, Dean W</creator><creator>Zare, Richard N</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20130507</creationdate><title>Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling</title><author>Perry, Richard H ; 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Chem</addtitle><date>2013-05-07</date><risdate>2013</risdate><volume>85</volume><issue>9</issue><spage>4259</spage><epage>4262</epage><pages>4259-4262</pages><issn>0003-2700</issn><issn>1520-6882</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model of MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach of combining DESI-MSI with the ability to turn specific genes on and off has led to the discovery of highly specific lipid molecules associated with MYC-induced tumor onset. We are able to distinguish normal from MYC-induced malignant cells. Our approach provides a strategy to define a precise molecular picture at a resolution of about 200 μm that may be useful in identifying lipid molecules that define how the MYC oncogene initiates and maintains tumorigenesis.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>23560736</pmid><doi>10.1021/ac400479j</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical chemistry animal models Animals carcinogenesis Carcinogenesis - genetics Carcinogenesis - metabolism Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology desorption Disease Models, Animal electrospray ionization mass spectrometry hepatoma image analysis lipid composition Lipid Metabolism - genetics Lipids Lipids - genetics Liver cancer Liver Neoplasms - genetics Liver Neoplasms - metabolism Liver Neoplasms - pathology Mass spectrometry Mice Mice, Transgenic Molecules oncogenes Proto-Oncogene Proteins c-myc - genetics Proto-Oncogene Proteins c-myc - metabolism Spectrometry, Mass, Electrospray Ionization tissues Transgenic animals |
| title | Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling |
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