Free and Immobilized Glucose Isomerase from Streptomyces phaeochromogenes1
Properties were determined of the glucose isomerase from Streptomyces phaeochromogenes NRRL B-3559. The enzyme exhibited a temperature optimum of 80 C and a p H optimum of about 8. The effect of various buffers on activity of the enzyme and the optimum p H were studied. Michaelis constants for gluco...
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| Veröffentlicht in: | Applied microbiology 1971, Vol.21 (4), p.588-593 |
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| container_title | Applied microbiology |
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| creator | Strandberg, Gerald W. Smiley, Karl L. |
| description | Properties were determined of the glucose isomerase from
Streptomyces phaeochromogenes
NRRL B-3559. The enzyme exhibited a temperature optimum of 80 C and a
p
H optimum of about 8. The effect of various buffers on activity of the enzyme and the optimum
p
H were studied. Michaelis constants for glucose and Mg
2+
were 0.25 and 0.025
m
, respectively. Co
2+
enhanced enzyme activity. A functional polyacrylamide-entrapped glucose isomerase was prepared. The conditions for entrapment and use of the bound enzyme were examined. |
| doi_str_mv | 10.1128/AEM.21.4.588-593.1971 |
| format | Article |
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Streptomyces phaeochromogenes
NRRL B-3559. The enzyme exhibited a temperature optimum of 80 C and a
p
H optimum of about 8. The effect of various buffers on activity of the enzyme and the optimum
p
H were studied. Michaelis constants for glucose and Mg
2+
were 0.25 and 0.025
m
, respectively. Co
2+
enhanced enzyme activity. A functional polyacrylamide-entrapped glucose isomerase was prepared. The conditions for entrapment and use of the bound enzyme were examined.</description><identifier>ISSN: 0003-6919</identifier><identifier>DOI: 10.1128/AEM.21.4.588-593.1971</identifier><identifier>PMID: 5575565</identifier><language>eng</language><subject>Metabolism and Products</subject><ispartof>Applied microbiology, 1971, Vol.21 (4), p.588-593</ispartof><rights>Copyright © 1971 American Society for Microbiology</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2651-d7f8c58aef2df90e077616ed1b6a766e38ee040ed6a3ae248123f82e838558b23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC377236/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC377236/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,4024,27923,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Strandberg, Gerald W.</creatorcontrib><creatorcontrib>Smiley, Karl L.</creatorcontrib><title>Free and Immobilized Glucose Isomerase from Streptomyces phaeochromogenes1</title><title>Applied microbiology</title><description>Properties were determined of the glucose isomerase from
Streptomyces phaeochromogenes
NRRL B-3559. The enzyme exhibited a temperature optimum of 80 C and a
p
H optimum of about 8. The effect of various buffers on activity of the enzyme and the optimum
p
H were studied. Michaelis constants for glucose and Mg
2+
were 0.25 and 0.025
m
, respectively. Co
2+
enhanced enzyme activity. A functional polyacrylamide-entrapped glucose isomerase was prepared. The conditions for entrapment and use of the bound enzyme were examined.</description><subject>Metabolism and Products</subject><issn>0003-6919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><recordid>eNpVkMtOwzAQRb0AlfL4BKT8QILHjh9ZsKiqthQVsQDWlpNM2qA4juwWqXw9qYqQWM3VXJ27OITcA80AmH6YLV4yBlmeCa1TUfAMCgUXZEop5aksoLgi1zF-Ugo548WETIRQQkgxJc_LgJjYvk7Wzvmy7dpvrJNVd6h8xGQdvcNgx9QE75K3fcBh792xwpgMO4u-2o1_v8UeI9ySy8Z2Ee9-7w35WC7e50_p5nW1ns82acWkgLRWja6EttiwuikoUqUkSKyhlFZJiVwj0pxiLS23yHINjDeaoeZaCF0yfkMez7vDoXRYV9jvg-3MEFpnw9F425r_Td_uzNZ_Ga4U43LkxZmvgo8xYPOHAjUnnWbUaRiY3Iw6zajTnHTyH1P3a7w</recordid><startdate>1971</startdate><enddate>1971</enddate><creator>Strandberg, Gerald W.</creator><creator>Smiley, Karl L.</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>1971</creationdate><title>Free and Immobilized Glucose Isomerase from Streptomyces phaeochromogenes1</title><author>Strandberg, Gerald W. ; Smiley, Karl L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2651-d7f8c58aef2df90e077616ed1b6a766e38ee040ed6a3ae248123f82e838558b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Metabolism and Products</topic><toplevel>online_resources</toplevel><creatorcontrib>Strandberg, Gerald W.</creatorcontrib><creatorcontrib>Smiley, Karl L.</creatorcontrib><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Strandberg, Gerald W.</au><au>Smiley, Karl L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Free and Immobilized Glucose Isomerase from Streptomyces phaeochromogenes1</atitle><jtitle>Applied microbiology</jtitle><date>1971</date><risdate>1971</risdate><volume>21</volume><issue>4</issue><spage>588</spage><epage>593</epage><pages>588-593</pages><issn>0003-6919</issn><abstract>Properties were determined of the glucose isomerase from
Streptomyces phaeochromogenes
NRRL B-3559. The enzyme exhibited a temperature optimum of 80 C and a
p
H optimum of about 8. The effect of various buffers on activity of the enzyme and the optimum
p
H were studied. Michaelis constants for glucose and Mg
2+
were 0.25 and 0.025
m
, respectively. Co
2+
enhanced enzyme activity. A functional polyacrylamide-entrapped glucose isomerase was prepared. The conditions for entrapment and use of the bound enzyme were examined.</abstract><pmid>5575565</pmid><doi>10.1128/AEM.21.4.588-593.1971</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-6919 |
| ispartof | Applied microbiology, 1971, Vol.21 (4), p.588-593 |
| issn | 0003-6919 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_377236 |
| source | American Society for Microbiology; PubMed Central |
| subjects | Metabolism and Products |
| title | Free and Immobilized Glucose Isomerase from Streptomyces phaeochromogenes1 |
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