Clathrin Coat Disassembly by the Yeast Hsc70/Ssa1p and Auxilin/Swa2p Proteins Observed by Single-particle Burst Analysis Spectroscopy

The role of clathrin-coated vesicles in receptor-mediated endocytosis is conserved among eukaryotes, and many of the proteins required for clathrin coat assembly and disassembly have orthologs in yeast and mammals. In yeast, dozens of proteins have been identified as regulators of the multistep reac...

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Veröffentlicht in:	The Journal of biological chemistry 2013-09, Vol.288 (37), p.26721-26730
Hauptverfasser:	Krantz, Kelly C., Puchalla, Jason, Thapa, Rajan, Kobayashi, Callie, Bisher, Margaret, Viehweg, Julie, Carr, Chavela M., Rye, Hays S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphatases - metabolism Adenosine Triphosphate - chemistry Auxilin Auxilins - metabolism Burst Analysis Spectroscopy Cell Biology Cell Membrane - metabolism Chromatography, Gel Clathrin Clathrin - metabolism Cytoplasm - metabolism Endocytosis Fluorescence Green Fluorescent Proteins - metabolism Hsc70 HSP70 Heat-Shock Proteins - metabolism Hydrolysis Membrane Trafficking Microscopy, Electron Molecular Chaperone Phosphoproteins - metabolism Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - metabolism Single-particle Analysis Spectrophotometry Vesicular Transport Proteins - metabolism
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