Sensitive, Site-Specific, and Stable Vibrational Probe of Local Protein Environments: 4‑Azidomethyl‑l‑Phenylalanine

We have synthesized the unnatural amino acid (UAA), 4-azidomethyl-l-phenylalanine (pN3CH2Phe), to serve as an effective vibrational reporter of local protein environments. The position, extinction coefficient, and sensitivity to local environment of the azide asymmetric stretch vibration of pN3CH2Ph...

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