The use of plasma-activated covalent attachment of early domains of tropoelastin to enhance vascular compatibility of surfaces
Abstract All current metallic vascular prostheses, including stents, exhibit suboptimal biocompatibility. Improving the re-endothelialization and reducing the thrombogenicity of these devices would substantially improve their clinical efficacy. Tropoelastin (TE), the soluble precursor of elastin, me...
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Veröffentlicht in: | Biomaterials 2013-10, Vol.34 (31), p.7584-7591 |
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creator | Hiob, Matti A Wise, Steven G Kondyurin, Alexey Waterhouse, Anna Bilek, Marcela M Ng, Martin K.C Weiss, Anthony S |
description | Abstract All current metallic vascular prostheses, including stents, exhibit suboptimal biocompatibility. Improving the re-endothelialization and reducing the thrombogenicity of these devices would substantially improve their clinical efficacy. Tropoelastin (TE), the soluble precursor of elastin, mediates favorable endothelial cell interactions while having low thrombogenicity. Here we show that constructs of TE corresponding to the first 10 (“N10”) and first 18 (“N18”) N-terminal domains of the molecule facilitate endothelial cell attachment and proliferation equivalent to the performance of full-length TE. This N-terminal ability contrasts with the known role of the C-terminus of TE in facilitating cell attachment, particularly of fibroblasts. When immobilized on a plasma-activated coating (“PAC”), N10 and N18 retained their bioactivity and endothelial cell interactive properties, demonstrating attachment and proliferation equivalent to full-length TE. In whole blood assays, both N10 and N18 maintained the low thrombogenicity of PAC. Furthermore, these N-terminal constructs displayed far greater resistance to protease degradation by blood serine proteases kallikrein and thrombin than did full-length TE. When immobilized onto a PAC surface, these shorter constructs form a modified metal interface to establish a platform technology for biologically compatible, implantable cardiovascular devices. |
doi_str_mv | 10.1016/j.biomaterials.2013.06.036 |
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Improving the re-endothelialization and reducing the thrombogenicity of these devices would substantially improve their clinical efficacy. Tropoelastin (TE), the soluble precursor of elastin, mediates favorable endothelial cell interactions while having low thrombogenicity. Here we show that constructs of TE corresponding to the first 10 (“N10”) and first 18 (“N18”) N-terminal domains of the molecule facilitate endothelial cell attachment and proliferation equivalent to the performance of full-length TE. This N-terminal ability contrasts with the known role of the C-terminus of TE in facilitating cell attachment, particularly of fibroblasts. When immobilized on a plasma-activated coating (“PAC”), N10 and N18 retained their bioactivity and endothelial cell interactive properties, demonstrating attachment and proliferation equivalent to full-length TE. In whole blood assays, both N10 and N18 maintained the low thrombogenicity of PAC. Furthermore, these N-terminal constructs displayed far greater resistance to protease degradation by blood serine proteases kallikrein and thrombin than did full-length TE. When immobilized onto a PAC surface, these shorter constructs form a modified metal interface to establish a platform technology for biologically compatible, implantable cardiovascular devices.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2013.06.036</identifier><identifier>PMID: 23863453</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Cell Proliferation ; Cells, Cultured ; Coated Materials, Biocompatible - chemistry ; Covalent immobilization ; Dentistry ; Electrophoresis, Polyacrylamide Gel ; Endothelial cell ; Endothelial Cells - cytology ; Fibroblasts - cytology ; Human Umbilical Vein Endothelial Cells - cytology ; Humans ; Male ; Microscopy, Electron, Scanning ; N-terminus ; Plasma surface modification ; Spectroscopy, Fourier Transform Infrared ; Stent ; Tropoelastin ; Tropoelastin - chemistry</subject><ispartof>Biomaterials, 2013-10, Vol.34 (31), p.7584-7591</ispartof><rights>Elsevier Ltd</rights><rights>2013 Elsevier Ltd</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><rights>2013 Elsevier Ltd. All rights reserved. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c542t-df94f1f92804eab3a0bad6548934731c3a2c6704ed1b11b8f973faf910f809f03</citedby><cites>FETCH-LOGICAL-c542t-df94f1f92804eab3a0bad6548934731c3a2c6704ed1b11b8f973faf910f809f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2013.06.036$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23863453$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hiob, Matti A</creatorcontrib><creatorcontrib>Wise, Steven G</creatorcontrib><creatorcontrib>Kondyurin, Alexey</creatorcontrib><creatorcontrib>Waterhouse, Anna</creatorcontrib><creatorcontrib>Bilek, Marcela M</creatorcontrib><creatorcontrib>Ng, Martin K.C</creatorcontrib><creatorcontrib>Weiss, Anthony S</creatorcontrib><title>The use of plasma-activated covalent attachment of early domains of tropoelastin to enhance vascular compatibility of surfaces</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract All current metallic vascular prostheses, including stents, exhibit suboptimal biocompatibility. Improving the re-endothelialization and reducing the thrombogenicity of these devices would substantially improve their clinical efficacy. Tropoelastin (TE), the soluble precursor of elastin, mediates favorable endothelial cell interactions while having low thrombogenicity. Here we show that constructs of TE corresponding to the first 10 (“N10”) and first 18 (“N18”) N-terminal domains of the molecule facilitate endothelial cell attachment and proliferation equivalent to the performance of full-length TE. This N-terminal ability contrasts with the known role of the C-terminus of TE in facilitating cell attachment, particularly of fibroblasts. When immobilized on a plasma-activated coating (“PAC”), N10 and N18 retained their bioactivity and endothelial cell interactive properties, demonstrating attachment and proliferation equivalent to full-length TE. In whole blood assays, both N10 and N18 maintained the low thrombogenicity of PAC. Furthermore, these N-terminal constructs displayed far greater resistance to protease degradation by blood serine proteases kallikrein and thrombin than did full-length TE. When immobilized onto a PAC surface, these shorter constructs form a modified metal interface to establish a platform technology for biologically compatible, implantable cardiovascular devices.</description><subject>Advanced Basic Science</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Covalent immobilization</subject><subject>Dentistry</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Endothelial cell</subject><subject>Endothelial Cells - cytology</subject><subject>Fibroblasts - cytology</subject><subject>Human Umbilical Vein Endothelial Cells - cytology</subject><subject>Humans</subject><subject>Male</subject><subject>Microscopy, Electron, Scanning</subject><subject>N-terminus</subject><subject>Plasma surface modification</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Stent</subject><subject>Tropoelastin</subject><subject>Tropoelastin - chemistry</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUk1v1DAQjRCIbgt_AUWcuGQZx3E-OFRCLRSkShwoZ2vijFkvTrzYTqS98NtxtKUqnDj5Y9578_Emy14z2DJg9dv9tjduxEjeoA3bEhjfQr0FXj_JNqxt2kJ0IJ5mG2BVWXQ1K8-y8xD2kN5Qlc-zs5K3Na8E32S_7naUz4Fyp_ODxTBigSqaJakPuXILWppijjGi2o3rNeEIvT3mQyrBTGH9iN4dHCV2NFMeXU7TDidF-YJBzRZ9EhoPGE1vrInHlRFmr1FReJE906kHenl_XmTfPn64u_pU3H65-Xz1_rZQoipjMeiu0kx3ZQsVYc8RehxqUbUdrxrOFMdS1U2KDaxnrG9113CNumOgW-g08Ivs8qR7mPuRBpU68WjlwZsR_VE6NPLvyGR28rtbJG9EK4AngTf3At79nClEOZqgyFqcyM1BsoqJGlrRdQn67gRV3oXgST-kYSBXA-VePjZQrgZKqGUyMJFfPS70gfrHsQS4PgEojWsx5GVQhtK0B-NJRTk48395Lv-RUdZMRqH9QUcKezf7aeUwGUoJ8uu6SusmMQ7QCCb4byJ2zKU</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Hiob, Matti A</creator><creator>Wise, Steven G</creator><creator>Kondyurin, Alexey</creator><creator>Waterhouse, Anna</creator><creator>Bilek, Marcela M</creator><creator>Ng, Martin K.C</creator><creator>Weiss, Anthony S</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20131001</creationdate><title>The use of plasma-activated covalent attachment of early domains of tropoelastin to enhance vascular compatibility of surfaces</title><author>Hiob, Matti A ; Wise, Steven G ; Kondyurin, Alexey ; Waterhouse, Anna ; Bilek, Marcela M ; Ng, Martin K.C ; Weiss, Anthony S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c542t-df94f1f92804eab3a0bad6548934731c3a2c6704ed1b11b8f973faf910f809f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Advanced Basic Science</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Covalent immobilization</topic><topic>Dentistry</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Endothelial cell</topic><topic>Endothelial Cells - cytology</topic><topic>Fibroblasts - cytology</topic><topic>Human Umbilical Vein Endothelial Cells - cytology</topic><topic>Humans</topic><topic>Male</topic><topic>Microscopy, Electron, Scanning</topic><topic>N-terminus</topic><topic>Plasma surface modification</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Stent</topic><topic>Tropoelastin</topic><topic>Tropoelastin - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hiob, Matti A</creatorcontrib><creatorcontrib>Wise, Steven G</creatorcontrib><creatorcontrib>Kondyurin, Alexey</creatorcontrib><creatorcontrib>Waterhouse, Anna</creatorcontrib><creatorcontrib>Bilek, Marcela M</creatorcontrib><creatorcontrib>Ng, Martin K.C</creatorcontrib><creatorcontrib>Weiss, Anthony S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hiob, Matti A</au><au>Wise, Steven G</au><au>Kondyurin, Alexey</au><au>Waterhouse, Anna</au><au>Bilek, Marcela M</au><au>Ng, Martin K.C</au><au>Weiss, Anthony S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The use of plasma-activated covalent attachment of early domains of tropoelastin to enhance vascular compatibility of surfaces</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>34</volume><issue>31</issue><spage>7584</spage><epage>7591</epage><pages>7584-7591</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract All current metallic vascular prostheses, including stents, exhibit suboptimal biocompatibility. Improving the re-endothelialization and reducing the thrombogenicity of these devices would substantially improve their clinical efficacy. Tropoelastin (TE), the soluble precursor of elastin, mediates favorable endothelial cell interactions while having low thrombogenicity. Here we show that constructs of TE corresponding to the first 10 (“N10”) and first 18 (“N18”) N-terminal domains of the molecule facilitate endothelial cell attachment and proliferation equivalent to the performance of full-length TE. This N-terminal ability contrasts with the known role of the C-terminus of TE in facilitating cell attachment, particularly of fibroblasts. When immobilized on a plasma-activated coating (“PAC”), N10 and N18 retained their bioactivity and endothelial cell interactive properties, demonstrating attachment and proliferation equivalent to full-length TE. In whole blood assays, both N10 and N18 maintained the low thrombogenicity of PAC. 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subjects | Advanced Basic Science Cell Proliferation Cells, Cultured Coated Materials, Biocompatible - chemistry Covalent immobilization Dentistry Electrophoresis, Polyacrylamide Gel Endothelial cell Endothelial Cells - cytology Fibroblasts - cytology Human Umbilical Vein Endothelial Cells - cytology Humans Male Microscopy, Electron, Scanning N-terminus Plasma surface modification Spectroscopy, Fourier Transform Infrared Stent Tropoelastin Tropoelastin - chemistry |
title | The use of plasma-activated covalent attachment of early domains of tropoelastin to enhance vascular compatibility of surfaces |
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