Structural basis for ribosomal 16S rRNA cleavage by the cytotoxic domain of colicin E3

Structural basis for ribosomal 16S rRNA cleavage by the cytotoxic domain of colicin E3

The toxin colicin E3 targets the 30S subunit of bacterial ribosomes and cleaves a phosphodiester bond in the decoding center. We present the crystal structure of the 70S ribosome in complex with the cytotoxic domain of colicin E3 (E3-rRNase). The structure reveals how the rRNase domain of colicin bi...

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Veröffentlicht in:Nature structural & molecular biology 2010-09, Vol.17 (10), p.1241-1246
Hauptverfasser: Ng, C Leong, Lang, Kathrin, Meenan, Nicola AG, Sharma, Amit, Kelley, Ann C, Kleanthous, Colin, Ramakrishnan, V
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container_issue 10
container_start_page 1241
container_title Nature structural & molecular biology
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creator Ng, C Leong
Lang, Kathrin
Meenan, Nicola AG
Sharma, Amit
Kelley, Ann C
Kleanthous, Colin
Ramakrishnan, V
description The toxin colicin E3 targets the 30S subunit of bacterial ribosomes and cleaves a phosphodiester bond in the decoding center. We present the crystal structure of the 70S ribosome in complex with the cytotoxic domain of colicin E3 (E3-rRNase). The structure reveals how the rRNase domain of colicin binds to the A site of the decoding center in the 70S ribosome and cleaves 16S rRNA between A1493 and G1494. The cleavage mechanism involves the concerted action of conserved residues Glu62 and His58 of the cytotoxic domain of colicin E3 that activate the 16S rRNA for 2′ OH induced hydrolysis. Conformational changes observed for E3-rRNase, 16S rRNA and Helix 69 of 23S rRNA suggest that a dynamic binding platform is required for colicin E3 binding and function.
doi_str_mv 10.1038/nsmb.1896
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title Structural basis for ribosomal 16S rRNA cleavage by the cytotoxic domain of colicin E3
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