A novel, multi-parallel, real-time polymerase chain reaction approach for eight gastrointestinal parasites provides improved diagnostic capabilities to resource-limited at-risk populations

Diagnosis of gastrointestinal parasites has traditionally relied on stool microscopy, which has low diagnostic sensitivity and specificity. We have developed a novel, rapid, high-throughput quantitative multi-parallel real-time polymerase chain reaction (qPCR) platform. Species-specific primers/prob...

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Veröffentlicht in:	The American journal of tropical medicine and hygiene 2013-06, Vol.88 (6), p.1041-1047
Hauptverfasser:	Mejia, Rojelio, Vicuña, Yosselin, Broncano, Nely, Sandoval, Carlos, Vaca, Maritza, Chico, Martha, Cooper, Philip J, Nutman, Thomas B
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Ancylostoma - isolation & purification Animals Anthelmintics - therapeutic use Ascaris Ascaris lumbricoides - isolation & purification Child Cryptosporidium Cryptosporidium - isolation & purification DNA Primers DNA, Helminth - isolation & purification Ecuador Empirical Research Entamoeba histolytica Entamoeba histolytica - isolation & purification Feces - parasitology Giardia lamblia Giardia lamblia - isolation & purification High-Throughput Screening Assays - methods Humans India Infant Intestinal Diseases, Parasitic - diagnosis Intestinal Diseases, Parasitic - drug therapy Intestinal Diseases, Parasitic - epidemiology Intestinal Diseases, Parasitic - parasitology Mali Microscopy - methods Necator americanus Necator americanus - isolation & purification North America Prevalence Real-Time Polymerase Chain Reaction - methods Reproducibility of Results Rural Population Sensitivity and Specificity Species Specificity Strongyloides stercoralis Strongyloides stercoralis - isolation & purification Trichuris - isolation & purification Trichuris trichiura
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