Physical Labeling of Papillomavirus-Infected, Immortal, and Cancerous Cervical Epithelial Cells Reveal Surface Changes at Immortal Stage
A significant change of surface features of malignant cervical epithelial cells compared to normal cells has been previously reported. Here, we are studying the question at which progressive stage leading to cervical cancer the surface alteration happens. A non-traditional method to identify maligna...
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| Veröffentlicht in: | Cell biochemistry and biophysics 2012-06, Vol.63 (2), p.109-116 |
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| container_title | Cell biochemistry and biophysics |
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| creator | Swaminathan Iyer, K. Gaikwad, R. M. Woodworth, C. D. Volkov, D. O. Sokolov, Igor |
| description | A significant change of surface features of malignant cervical epithelial cells compared to normal cells has been previously reported. Here, we are studying the question at which progressive stage leading to cervical cancer the surface alteration happens. A non-traditional method to identify malignant cervical epithelial cells in vitro
,
which is based on physical (in contrast to specific biochemical) labelling of cells with fluorescent silica micron-size beads, is used here to examine cells at progressive stages leading to cervical cancer which include normal epithelial cells, cells infected with human papillomavirus type-16 (HPV-16), cells immortalized by HPV-16, and carcinoma cells. The study shows a statistically significant (at
p
|
| doi_str_mv | 10.1007/s12013-012-9345-2 |
| format | Article |
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,
which is based on physical (in contrast to specific biochemical) labelling of cells with fluorescent silica micron-size beads, is used here to examine cells at progressive stages leading to cervical cancer which include normal epithelial cells, cells infected with human papillomavirus type-16 (HPV-16), cells immortalized by HPV-16, and carcinoma cells. The study shows a statistically significant (at
p
< 0.01) difference between both immortal and cancer cells and a group consisting of normal and infected. There is no significant difference between normal and infected cells. Immortal cells demonstrate the signal which is closer to cancer cells than to either normal or infected cells. This implies that the cell surface, surface cellular brush changes substantially when cells become immortal. Physical labeling of the cell surface represents a substantial departure from the traditional biochemical labeling methods. The results presented show the potential significance of physical properties of the cell surface for development of clinical methods for early detection of cervical cancer, even at the stage of immortalized, premalignant cells.</description><identifier>ISSN: 1085-9195</identifier><identifier>EISSN: 1559-0283</identifier><identifier>DOI: 10.1007/s12013-012-9345-2</identifier><identifier>PMID: 22351422</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Biochemistry ; Biological and Medical Physics ; Biomedical and Life Sciences ; Biophysics ; Biotechnology ; Carcinoma ; Cell Adhesion ; Cell Biology ; Cell surface ; Cell Transformation, Viral ; Cervical cancer ; Cervix ; Epithelial cells ; Epithelial Cells - pathology ; Epithelial Cells - virology ; Female ; Fluorescent Dyes - chemistry ; Human papillomavirus ; Human papillomavirus 16 ; Humans ; Life Sciences ; Microscopy, Fluorescence ; Microspheres ; Original Paper ; Papillomavirus Infections - pathology ; Papillomavirus Infections - virology ; Pharmacology/Toxicology ; Physical properties ; Rhodamines - chemistry ; Silica ; Staining and Labeling ; Statistical analysis ; Tumor Cells, Cultured ; Uterine Cervical Neoplasms - pathology ; Uterine Cervical Neoplasms - virology</subject><ispartof>Cell biochemistry and biophysics, 2012-06, Vol.63 (2), p.109-116</ispartof><rights>Springer Science+Business Media, LLC 2012</rights><rights>Springer Science+Business Media, LLC 2012 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-f01c4e92f6660ff0d2589b28eb7cfed31955a2f91a7c1464f89cc10ba7250a63</citedby><cites>FETCH-LOGICAL-c503t-f01c4e92f6660ff0d2589b28eb7cfed31955a2f91a7c1464f89cc10ba7250a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12013-012-9345-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12013-012-9345-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22351422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Swaminathan Iyer, K.</creatorcontrib><creatorcontrib>Gaikwad, R. M.</creatorcontrib><creatorcontrib>Woodworth, C. D.</creatorcontrib><creatorcontrib>Volkov, D. O.</creatorcontrib><creatorcontrib>Sokolov, Igor</creatorcontrib><title>Physical Labeling of Papillomavirus-Infected, Immortal, and Cancerous Cervical Epithelial Cells Reveal Surface Changes at Immortal Stage</title><title>Cell biochemistry and biophysics</title><addtitle>Cell Biochem Biophys</addtitle><addtitle>Cell Biochem Biophys</addtitle><description>A significant change of surface features of malignant cervical epithelial cells compared to normal cells has been previously reported. Here, we are studying the question at which progressive stage leading to cervical cancer the surface alteration happens. A non-traditional method to identify malignant cervical epithelial cells in vitro
,
which is based on physical (in contrast to specific biochemical) labelling of cells with fluorescent silica micron-size beads, is used here to examine cells at progressive stages leading to cervical cancer which include normal epithelial cells, cells infected with human papillomavirus type-16 (HPV-16), cells immortalized by HPV-16, and carcinoma cells. The study shows a statistically significant (at
p
< 0.01) difference between both immortal and cancer cells and a group consisting of normal and infected. There is no significant difference between normal and infected cells. Immortal cells demonstrate the signal which is closer to cancer cells than to either normal or infected cells. This implies that the cell surface, surface cellular brush changes substantially when cells become immortal. Physical labeling of the cell surface represents a substantial departure from the traditional biochemical labeling methods. The results presented show the potential significance of physical properties of the cell surface for development of clinical methods for early detection of cervical cancer, even at the stage of immortalized, premalignant cells.</description><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>Carcinoma</subject><subject>Cell Adhesion</subject><subject>Cell Biology</subject><subject>Cell surface</subject><subject>Cell Transformation, Viral</subject><subject>Cervical cancer</subject><subject>Cervix</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - pathology</subject><subject>Epithelial Cells - virology</subject><subject>Female</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Human papillomavirus</subject><subject>Human papillomavirus 16</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Microscopy, Fluorescence</subject><subject>Microspheres</subject><subject>Original Paper</subject><subject>Papillomavirus Infections - pathology</subject><subject>Papillomavirus Infections - virology</subject><subject>Pharmacology/Toxicology</subject><subject>Physical properties</subject><subject>Rhodamines - chemistry</subject><subject>Silica</subject><subject>Staining and Labeling</subject><subject>Statistical analysis</subject><subject>Tumor Cells, Cultured</subject><subject>Uterine Cervical Neoplasms - pathology</subject><subject>Uterine Cervical Neoplasms - virology</subject><issn>1085-9195</issn><issn>1559-0283</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kcFu1DAQhiMEoqXwAFyQJS4cGhg7dhJfkFBUYKWVqGjv1sQZ76ZyksVOVuob8Nh42bIqSJw81vzz2f_8Wfaaw3sOUH2IXAAvcuAi14VUuXiSnXOldA6iLp6mGmqVa67VWfYixjsAIUDK59mZEIXiUojz7Of19j72Fj1bY0u-Hzdscuwad73304D7PiwxX42O7EzdJVsNwxRm9JcMx441OFoK0xJZQ2H_m3K16-dt4qSyIe8j-057SpebJTi0xJotjhuKDOcTi93MuKGX2TOHPtKrh_Miu_18ddt8zdffvqyaT-vcKijm3AG3krRwZVmCc9AJVetW1NRW1lFXJLMKhdMcK8tlKV2treXQYiUUYFlcZB-P2N3SDtRZGueA3uxCP2C4NxP25u_O2G_NZtqbopIlrw-Adw-AMP1YKM5m6KNNVnGktAnDQUAtOWiVpG__kd5NSxiTu6TiRa0kaJFU_KiyYYoxkDt9hoM5xGyOMZsUsznEbA4zbx67OE38yTUJxFEQUystPDx--n_UX9PatFY</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Swaminathan Iyer, K.</creator><creator>Gaikwad, R. 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M.</au><au>Woodworth, C. D.</au><au>Volkov, D. O.</au><au>Sokolov, Igor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physical Labeling of Papillomavirus-Infected, Immortal, and Cancerous Cervical Epithelial Cells Reveal Surface Changes at Immortal Stage</atitle><jtitle>Cell biochemistry and biophysics</jtitle><stitle>Cell Biochem Biophys</stitle><addtitle>Cell Biochem Biophys</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>63</volume><issue>2</issue><spage>109</spage><epage>116</epage><pages>109-116</pages><issn>1085-9195</issn><eissn>1559-0283</eissn><abstract>A significant change of surface features of malignant cervical epithelial cells compared to normal cells has been previously reported. Here, we are studying the question at which progressive stage leading to cervical cancer the surface alteration happens. A non-traditional method to identify malignant cervical epithelial cells in vitro
,
which is based on physical (in contrast to specific biochemical) labelling of cells with fluorescent silica micron-size beads, is used here to examine cells at progressive stages leading to cervical cancer which include normal epithelial cells, cells infected with human papillomavirus type-16 (HPV-16), cells immortalized by HPV-16, and carcinoma cells. The study shows a statistically significant (at
p
< 0.01) difference between both immortal and cancer cells and a group consisting of normal and infected. There is no significant difference between normal and infected cells. Immortal cells demonstrate the signal which is closer to cancer cells than to either normal or infected cells. This implies that the cell surface, surface cellular brush changes substantially when cells become immortal. Physical labeling of the cell surface represents a substantial departure from the traditional biochemical labeling methods. The results presented show the potential significance of physical properties of the cell surface for development of clinical methods for early detection of cervical cancer, even at the stage of immortalized, premalignant cells.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>22351422</pmid><doi>10.1007/s12013-012-9345-2</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biophysics Biotechnology Carcinoma Cell Adhesion Cell Biology Cell surface Cell Transformation, Viral Cervical cancer Cervix Epithelial cells Epithelial Cells - pathology Epithelial Cells - virology Female Fluorescent Dyes - chemistry Human papillomavirus Human papillomavirus 16 Humans Life Sciences Microscopy, Fluorescence Microspheres Original Paper Papillomavirus Infections - pathology Papillomavirus Infections - virology Pharmacology/Toxicology Physical properties Rhodamines - chemistry Silica Staining and Labeling Statistical analysis Tumor Cells, Cultured Uterine Cervical Neoplasms - pathology Uterine Cervical Neoplasms - virology |
| title | Physical Labeling of Papillomavirus-Infected, Immortal, and Cancerous Cervical Epithelial Cells Reveal Surface Changes at Immortal Stage |
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