FAS and FAS-L Genotype and Expression in Patients With Recurrent Pregnancy Loss
We assessed FAS and FAS-L gene polymorphisms and messenger RNA (mRNA) levels in patients with recurrent pregnancy loss (RPL). This case–control study compared 129 women with RPL with 235 healthy multiparous women (control group). Genomic DNA and total mRNA were extracted from whole blood, and polymo...
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| Veröffentlicht in: | Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2013-09, Vol.20 (9), p.1111-1115 |
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| creator | Banzato, Priscilla Chamelete Andrade Daher, Silvia Traina, Évelyn Torloni, Maria Regina Gueuvoghlanian-Silva, Bárbara Yasmin Puccini, Renata Fiorini Pendeloski, Karen Priscilla Tezotto Mattar, Rosiane |
| description | We assessed FAS and FAS-L gene polymorphisms and messenger RNA (mRNA) levels in patients with recurrent pregnancy loss (RPL). This case–control study compared 129 women with RPL with 235 healthy multiparous women (control group). Genomic DNA and total mRNA were extracted from whole blood, and polymorphisms genotyping was performed by polymerase chain reaction (PCR). Messenger RNA expression levels were analyzed by real-time PCR. Data were analyzed by chi-square and Fisher exact tests; P < .05 was considered significant. There were no significant differences in the FAS (670 A/G) genotype or allelic frequencies between the RPL and control groups. We found significant differences in the FAS-L (844 C/T) genotype and allelic frequencies between women with RPL and controls. Patients with RPL had significantly higher FAS-L expression. Our data suggest that FAS-L gene polymorphism is associated with increased susceptibility to RPL. Moreover, women with RPL seem to abnormally express FAS-FAS-L molecules. |
| doi_str_mv | 10.1177/1933719113477488 |
| format | Article |
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This case–control study compared 129 women with RPL with 235 healthy multiparous women (control group). Genomic DNA and total mRNA were extracted from whole blood, and polymorphisms genotyping was performed by polymerase chain reaction (PCR). Messenger RNA expression levels were analyzed by real-time PCR. Data were analyzed by chi-square and Fisher exact tests; P < .05 was considered significant. There were no significant differences in the FAS (670 A/G) genotype or allelic frequencies between the RPL and control groups. We found significant differences in the FAS-L (844 C/T) genotype and allelic frequencies between women with RPL and controls. Patients with RPL had significantly higher FAS-L expression. Our data suggest that FAS-L gene polymorphism is associated with increased susceptibility to RPL. Moreover, women with RPL seem to abnormally express FAS-FAS-L molecules.</description><identifier>ISSN: 1933-7191</identifier><identifier>EISSN: 1933-7205</identifier><identifier>DOI: 10.1177/1933719113477488</identifier><identifier>PMID: 23420824</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Abortion, Habitual - genetics ; Adult ; Brazil ; Case-Control Studies ; Chi-Square Distribution ; Embryology ; Fas Ligand Protein - genetics ; fas Receptor - genetics ; Female ; Gene Expression Regulation ; Gene Frequency ; Genetic Predisposition to Disease ; Humans ; Medicine & Public Health ; Obstetrics/Perinatology/Midwifery ; Original ; Original Article ; Phenotype ; Polymorphism, Genetic ; Pregnancy ; Real-Time Polymerase Chain Reaction ; Reproductive Medicine ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Young Adult</subject><ispartof>Reproductive sciences (Thousand Oaks, Calif.), 2013-09, Vol.20 (9), p.1111-1115</ispartof><rights>The Author(s) 2013</rights><rights>Society for Reproductive Investigation 2013</rights><rights>The Author(s) 2013 2013 Society for Gynecologic Investigation</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-5096ac4a009b170bcf1510ec1c769259ba43a7beaa5419b2eb020f8c60b9bad93</citedby><cites>FETCH-LOGICAL-c476t-5096ac4a009b170bcf1510ec1c769259ba43a7beaa5419b2eb020f8c60b9bad93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1933719113477488$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1933719113477488$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>230,314,780,784,885,21819,27924,27925,41488,42557,43621,43622,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23420824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Banzato, Priscilla Chamelete Andrade</creatorcontrib><creatorcontrib>Daher, Silvia</creatorcontrib><creatorcontrib>Traina, Évelyn</creatorcontrib><creatorcontrib>Torloni, Maria Regina</creatorcontrib><creatorcontrib>Gueuvoghlanian-Silva, Bárbara Yasmin</creatorcontrib><creatorcontrib>Puccini, Renata Fiorini</creatorcontrib><creatorcontrib>Pendeloski, Karen Priscilla Tezotto</creatorcontrib><creatorcontrib>Mattar, Rosiane</creatorcontrib><title>FAS and FAS-L Genotype and Expression in Patients With Recurrent Pregnancy Loss</title><title>Reproductive sciences (Thousand Oaks, Calif.)</title><addtitle>Reprod. Sci</addtitle><addtitle>Reprod Sci</addtitle><description>We assessed FAS and FAS-L gene polymorphisms and messenger RNA (mRNA) levels in patients with recurrent pregnancy loss (RPL). This case–control study compared 129 women with RPL with 235 healthy multiparous women (control group). Genomic DNA and total mRNA were extracted from whole blood, and polymorphisms genotyping was performed by polymerase chain reaction (PCR). Messenger RNA expression levels were analyzed by real-time PCR. Data were analyzed by chi-square and Fisher exact tests; P < .05 was considered significant. There were no significant differences in the FAS (670 A/G) genotype or allelic frequencies between the RPL and control groups. We found significant differences in the FAS-L (844 C/T) genotype and allelic frequencies between women with RPL and controls. Patients with RPL had significantly higher FAS-L expression. Our data suggest that FAS-L gene polymorphism is associated with increased susceptibility to RPL. Moreover, women with RPL seem to abnormally express FAS-FAS-L molecules.</description><subject>Abortion, Habitual - genetics</subject><subject>Adult</subject><subject>Brazil</subject><subject>Case-Control Studies</subject><subject>Chi-Square Distribution</subject><subject>Embryology</subject><subject>Fas Ligand Protein - genetics</subject><subject>fas Receptor - genetics</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>Gene Frequency</subject><subject>Genetic Predisposition to Disease</subject><subject>Humans</subject><subject>Medicine & Public Health</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Original</subject><subject>Original Article</subject><subject>Phenotype</subject><subject>Polymorphism, Genetic</subject><subject>Pregnancy</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Reproductive Medicine</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Young Adult</subject><issn>1933-7191</issn><issn>1933-7205</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUUtLAzEQDqJYX3dPkqOX1Uw2u9lchCJtFQqKDzyGbDqtK222Jrti_72prUU9iKd5fI9MZgg5BnYGIOU5qDSVoABSIaUoii2yt2wlkrNs-yuPeIfsh_DCWCYUL3ZJh6eCs4KLPXLT795T40Y0xmRIB-jqZjHHz1bvfe4xhKp2tHL01jQVuibQp6p5pndoW-9jTW89TpxxdkGHdQiHZGdspgGP1vGAPPZ7D5dXyfBmcH3ZHSZWyLxJMqZyY4VhTJUgWWnHkAFDC1bmimeqNCI1skRjMgGq5FgyzsaFzVkZsZFKD8jFynfeljMc2TiJN1M999XM-IWuTaV_Iq561pP6TadSZBLyaHC6NvD1a4uh0bMqWJxOjcO6DRoEzzPIlYBIZSuq9fGHHsebZ4Dp5R307ztEycn38TaCr8VHAqwIIUJugl6_1K13cWV_mSZrjZngP_gfPaWerg</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Banzato, Priscilla Chamelete Andrade</creator><creator>Daher, Silvia</creator><creator>Traina, Évelyn</creator><creator>Torloni, Maria Regina</creator><creator>Gueuvoghlanian-Silva, Bárbara Yasmin</creator><creator>Puccini, Renata Fiorini</creator><creator>Pendeloski, Karen Priscilla Tezotto</creator><creator>Mattar, Rosiane</creator><general>SAGE Publications</general><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130901</creationdate><title>FAS and FAS-L Genotype and Expression in Patients With Recurrent Pregnancy Loss</title><author>Banzato, Priscilla Chamelete Andrade ; Daher, Silvia ; Traina, Évelyn ; Torloni, Maria Regina ; Gueuvoghlanian-Silva, Bárbara Yasmin ; Puccini, Renata Fiorini ; Pendeloski, Karen Priscilla Tezotto ; Mattar, Rosiane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-5096ac4a009b170bcf1510ec1c769259ba43a7beaa5419b2eb020f8c60b9bad93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Abortion, Habitual - genetics</topic><topic>Adult</topic><topic>Brazil</topic><topic>Case-Control Studies</topic><topic>Chi-Square Distribution</topic><topic>Embryology</topic><topic>Fas Ligand Protein - genetics</topic><topic>fas Receptor - genetics</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>Gene Frequency</topic><topic>Genetic Predisposition to Disease</topic><topic>Humans</topic><topic>Medicine & Public Health</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Original</topic><topic>Original Article</topic><topic>Phenotype</topic><topic>Polymorphism, Genetic</topic><topic>Pregnancy</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Reproductive Medicine</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Banzato, Priscilla Chamelete Andrade</creatorcontrib><creatorcontrib>Daher, Silvia</creatorcontrib><creatorcontrib>Traina, Évelyn</creatorcontrib><creatorcontrib>Torloni, Maria Regina</creatorcontrib><creatorcontrib>Gueuvoghlanian-Silva, Bárbara Yasmin</creatorcontrib><creatorcontrib>Puccini, Renata Fiorini</creatorcontrib><creatorcontrib>Pendeloski, Karen Priscilla Tezotto</creatorcontrib><creatorcontrib>Mattar, Rosiane</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Reproductive sciences (Thousand Oaks, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Banzato, Priscilla Chamelete Andrade</au><au>Daher, Silvia</au><au>Traina, Évelyn</au><au>Torloni, Maria Regina</au><au>Gueuvoghlanian-Silva, Bárbara Yasmin</au><au>Puccini, Renata Fiorini</au><au>Pendeloski, Karen Priscilla Tezotto</au><au>Mattar, Rosiane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>FAS and FAS-L Genotype and Expression in Patients With Recurrent Pregnancy Loss</atitle><jtitle>Reproductive sciences (Thousand Oaks, Calif.)</jtitle><stitle>Reprod. Sci</stitle><addtitle>Reprod Sci</addtitle><date>2013-09-01</date><risdate>2013</risdate><volume>20</volume><issue>9</issue><spage>1111</spage><epage>1115</epage><pages>1111-1115</pages><issn>1933-7191</issn><eissn>1933-7205</eissn><abstract>We assessed FAS and FAS-L gene polymorphisms and messenger RNA (mRNA) levels in patients with recurrent pregnancy loss (RPL). This case–control study compared 129 women with RPL with 235 healthy multiparous women (control group). Genomic DNA and total mRNA were extracted from whole blood, and polymorphisms genotyping was performed by polymerase chain reaction (PCR). Messenger RNA expression levels were analyzed by real-time PCR. Data were analyzed by chi-square and Fisher exact tests; P < .05 was considered significant. There were no significant differences in the FAS (670 A/G) genotype or allelic frequencies between the RPL and control groups. We found significant differences in the FAS-L (844 C/T) genotype and allelic frequencies between women with RPL and controls. Patients with RPL had significantly higher FAS-L expression. Our data suggest that FAS-L gene polymorphism is associated with increased susceptibility to RPL. Moreover, women with RPL seem to abnormally express FAS-FAS-L molecules.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>23420824</pmid><doi>10.1177/1933719113477488</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Abortion, Habitual - genetics Adult Brazil Case-Control Studies Chi-Square Distribution Embryology Fas Ligand Protein - genetics fas Receptor - genetics Female Gene Expression Regulation Gene Frequency Genetic Predisposition to Disease Humans Medicine & Public Health Obstetrics/Perinatology/Midwifery Original Original Article Phenotype Polymorphism, Genetic Pregnancy Real-Time Polymerase Chain Reaction Reproductive Medicine Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Young Adult |
| title | FAS and FAS-L Genotype and Expression in Patients With Recurrent Pregnancy Loss |
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