Mouse sperm acquire a new structure on the apical hook during epididymal maturation
Spermatozoa emerging from the testis undergo a maturation process in the epididymis during which they change morphologically, biochemically and physiologically to gain motility and the ability to fertilize ova. We examined mouse epididymal sperm with immunostaining and transmission electron microsco...
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| Veröffentlicht in: | Asian journal of andrology 2013-07, Vol.15 (4), p.523-528 |
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| creator | Lin, Yi-Wen Hsu, Tzu-Han Yen, Pauline H |
| description | Spermatozoa emerging from the testis undergo a maturation process in the epididymis during which they change morphologically, biochemically and physiologically to gain motility and the ability to fertilize ova. We examined mouse epididymal sperm with immunostaining and transmission electron microscopy (EM) and identified a previously unknown structure on the apical hook. The structure has a coiled configuration around 11 nm in thickness and is present at the tip of each corner of the triangular-rod shaped perforatorium. Surveying sperm isolated from various regions of the epididymis indicated that mouse sperm acquire the hook rim (HR) structure during its passage through the proximal two-thirds of the caput epididymidis. The structure withstands vigorous sonication and harsh chemical treatments and remains intact after the acrosome reaction. Its location and sturdiness suggest a function in protecting the apical hook from mechanical wear during fertilization. Our EM images of epididymal sperm also revealed additional novel structures as well as lateral asymmetry of the sperm head, indicating that mouse sperm head has a structure more complex than previously recognized. |
| doi_str_mv | 10.1038/aja.2013.46 |
| format | Article |
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We examined mouse epididymal sperm with immunostaining and transmission electron microscopy (EM) and identified a previously unknown structure on the apical hook. The structure has a coiled configuration around 11 nm in thickness and is present at the tip of each corner of the triangular-rod shaped perforatorium. Surveying sperm isolated from various regions of the epididymis indicated that mouse sperm acquire the hook rim (HR) structure during its passage through the proximal two-thirds of the caput epididymidis. The structure withstands vigorous sonication and harsh chemical treatments and remains intact after the acrosome reaction. Its location and sturdiness suggest a function in protecting the apical hook from mechanical wear during fertilization. Our EM images of epididymal sperm also revealed additional novel structures as well as lateral asymmetry of the sperm head, indicating that mouse sperm head has a structure more complex than previously recognized.</description><identifier>ISSN: 1008-682X</identifier><identifier>EISSN: 1745-7262</identifier><identifier>DOI: 10.1038/aja.2013.46</identifier><identifier>PMID: 23728587</identifier><language>eng</language><publisher>China: Medknow Publications & Media Pvt. Ltd</publisher><subject>Acrosome Reaction - physiology ; Animals ; Cell Differentiation - physiology ; Epididymis - physiology ; Immunohistochemistry ; Male ; Mice ; Mice, Inbred C57BL ; Microscopy, Electron, Transmission ; Original ; Sonication ; Sperm Head - physiology ; Sperm Head - ultrastructure ; Sperm Maturation - physiology ; Spermatozoa - cytology ; Spermatozoa - physiology ; Stress, Mechanical</subject><ispartof>Asian journal of andrology, 2013-07, Vol.15 (4), p.523-528</ispartof><rights>Copyright Nature Publishing Group Jul 2013</rights><rights>Copyright © 2013 SIMM & SJTU 2013 SIMM & SJTU</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3739244/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3739244/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23728587$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Yi-Wen</creatorcontrib><creatorcontrib>Hsu, Tzu-Han</creatorcontrib><creatorcontrib>Yen, Pauline H</creatorcontrib><title>Mouse sperm acquire a new structure on the apical hook during epididymal maturation</title><title>Asian journal of andrology</title><addtitle>Asian J Androl</addtitle><description>Spermatozoa emerging from the testis undergo a maturation process in the epididymis during which they change morphologically, biochemically and physiologically to gain motility and the ability to fertilize ova. We examined mouse epididymal sperm with immunostaining and transmission electron microscopy (EM) and identified a previously unknown structure on the apical hook. The structure has a coiled configuration around 11 nm in thickness and is present at the tip of each corner of the triangular-rod shaped perforatorium. Surveying sperm isolated from various regions of the epididymis indicated that mouse sperm acquire the hook rim (HR) structure during its passage through the proximal two-thirds of the caput epididymidis. The structure withstands vigorous sonication and harsh chemical treatments and remains intact after the acrosome reaction. Its location and sturdiness suggest a function in protecting the apical hook from mechanical wear during fertilization. Our EM images of epididymal sperm also revealed additional novel structures as well as lateral asymmetry of the sperm head, indicating that mouse sperm head has a structure more complex than previously recognized.</description><subject>Acrosome Reaction - physiology</subject><subject>Animals</subject><subject>Cell Differentiation - physiology</subject><subject>Epididymis - physiology</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microscopy, Electron, Transmission</subject><subject>Original</subject><subject>Sonication</subject><subject>Sperm Head - physiology</subject><subject>Sperm Head - ultrastructure</subject><subject>Sperm Maturation - physiology</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - physiology</subject><subject>Stress, Mechanical</subject><issn>1008-682X</issn><issn>1745-7262</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpVkM1LxDAQxYMo7rp68i4Bz13z0TbJRZDFL1jxoIK3kqbT3azbpps2yv73RlxFTzPz5vGb4SF0SsmUEi4v9EpPGaF8muZ7aExFmiWC5Ww_9oTIJJfsdYSO-n5FCONUqUM0YlwwmUkxRk8PLvSA-w58g7XZBOsBa9zCB-4HH8wQ4uxaPCyj3Fmj13jp3BuugrftAkNnK1ttmyg3Onr1YF17jA5qve7hZFcn6OXm-nl2l8wfb-9nV_Oko4oOiclBMSVTVvGyNlTmzEDNSQ415FBlNZdUK1lqRUoujNSQUV7XJRFRTklK-ARdfnO7UDZQGWgHr9dF522j_bZw2hb_N61dFgv3XnDBFUvTCDjfAbzbBOiHYuWCb-PPBY0JiUxQ_uU6-3vml_8TIv8E9nB1Zw</recordid><startdate>20130701</startdate><enddate>20130701</enddate><creator>Lin, Yi-Wen</creator><creator>Hsu, Tzu-Han</creator><creator>Yen, Pauline H</creator><general>Medknow Publications & Media Pvt. 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We examined mouse epididymal sperm with immunostaining and transmission electron microscopy (EM) and identified a previously unknown structure on the apical hook. The structure has a coiled configuration around 11 nm in thickness and is present at the tip of each corner of the triangular-rod shaped perforatorium. Surveying sperm isolated from various regions of the epididymis indicated that mouse sperm acquire the hook rim (HR) structure during its passage through the proximal two-thirds of the caput epididymidis. The structure withstands vigorous sonication and harsh chemical treatments and remains intact after the acrosome reaction. Its location and sturdiness suggest a function in protecting the apical hook from mechanical wear during fertilization. Our EM images of epididymal sperm also revealed additional novel structures as well as lateral asymmetry of the sperm head, indicating that mouse sperm head has a structure more complex than previously recognized.</abstract><cop>China</cop><pub>Medknow Publications & Media Pvt. Ltd</pub><pmid>23728587</pmid><doi>10.1038/aja.2013.46</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Acrosome Reaction - physiology Animals Cell Differentiation - physiology Epididymis - physiology Immunohistochemistry Male Mice Mice, Inbred C57BL Microscopy, Electron, Transmission Original Sonication Sperm Head - physiology Sperm Head - ultrastructure Sperm Maturation - physiology Spermatozoa - cytology Spermatozoa - physiology Stress, Mechanical |
| title | Mouse sperm acquire a new structure on the apical hook during epididymal maturation |
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