Kinetic Mechanism of Translocation and dNTP Binding in Individual DNA Polymerase Complexes

Complexes formed between phi29 DNA polymerase (DNAP) and DNA fluctuate discretely between the pre-translocation and post-translocation states on the millisecond time scale. The translocation fluctuations can be observed in ionic current traces when individual complexes are captured atop the α-hemoly...

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Veröffentlicht in:	Journal of the American Chemical Society 2013-06, Vol.135 (24), p.9149-9155
Hauptverfasser:	Lieberman, Kate R, Dahl, Joseph M, Mai, Ai H, Cox, Ashley, Akeson, Mark, Wang, Hongyun
Format:	Artikel
Sprache:	eng
Schlagworte:	active sites Bacillus Phages - enzymology Bacillus Phages - metabolism Base Sequence dissociation DNA DNA - metabolism DNA-directed DNA polymerase DNA-Directed DNA Polymerase - metabolism electric field hemolysins Kinetics nanopores Nucleotides - metabolism
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