Variations at a quantitative trait locus (QTL) affect development of behavior in lead-exposed Drosophila melanogaster
We developed Drosophila melanogaster as a model to study correlated behavioral, neuronal and genetic effects of the neurotoxin lead, known to affect cognitive and behavioral development in children. We showed that, as in vertebrates, lead affects both synaptic development and complex behaviors (cour...
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| Veröffentlicht in: | Neurotoxicology (Park Forest South) 2009-03, Vol.30 (2), p.305-311 |
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| creator | Hirsch, Helmut V.B. Possidente, Debra Averill, Sarah Despain, Tamira Palmetto Buytkins, Joel Thomas, Valerie Goebel, W. Paul Shipp-Hilts, Asante Wilson, Diane Hollocher, Kurt Possidente, Bernard Lnenicka, Greg Ruden, Douglas M. |
| description | We developed
Drosophila melanogaster as a model to study correlated behavioral, neuronal and genetic effects of the neurotoxin lead, known to affect cognitive and behavioral development in children. We showed that, as in vertebrates, lead affects both synaptic development and complex behaviors (courtship, fecundity, locomotor activity) in
Drosophila. By assessing differential behavioral responses to developmental lead exposure among recombinant inbred
Drosophila lines (RI), derived from parental lines Oregon R and Russian 2b, we have now identified a genotype by environment interaction (GEI) for a behavioral trait affected by lead. Drosophila Activity Monitors (TriKinetics, Waltham, MA), which measure activity by counting the number of times a single fly in a small glass tube walks through an infrared beam aimed at the middle of the tube, were used to measure activity of flies, reared from eggs to 4 days of adult age on either control or lead-contaminated medium, from each of 75 RI lines. We observed a significant statistical association between the effect of lead on Average Daytime Activity (ADA) across lines and one marker locus, 30AB, on chromosome 2; we define this as a Quantitative Trait Locus (QTL) associated with behavioral effects of developmental lead exposure. When 30AB was from Russian 2b, lead significantly increased locomotor activity, whereas, when 30AB was from Oregon R, lead decreased it. 30AB contains about 125 genes among which are likely “candidate genes” for the observed lead-dependent behavioral changes.
Drosophila are thus a useful, underutilized model for studying behavioral, synaptic and genetic changes following chronic exposure to lead or other neurotoxins during development. |
| doi_str_mv | 10.1016/j.neuro.2009.01.004 |
| format | Article |
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Drosophila melanogaster as a model to study correlated behavioral, neuronal and genetic effects of the neurotoxin lead, known to affect cognitive and behavioral development in children. We showed that, as in vertebrates, lead affects both synaptic development and complex behaviors (courtship, fecundity, locomotor activity) in
Drosophila. By assessing differential behavioral responses to developmental lead exposure among recombinant inbred
Drosophila lines (RI), derived from parental lines Oregon R and Russian 2b, we have now identified a genotype by environment interaction (GEI) for a behavioral trait affected by lead. Drosophila Activity Monitors (TriKinetics, Waltham, MA), which measure activity by counting the number of times a single fly in a small glass tube walks through an infrared beam aimed at the middle of the tube, were used to measure activity of flies, reared from eggs to 4 days of adult age on either control or lead-contaminated medium, from each of 75 RI lines. We observed a significant statistical association between the effect of lead on Average Daytime Activity (ADA) across lines and one marker locus, 30AB, on chromosome 2; we define this as a Quantitative Trait Locus (QTL) associated with behavioral effects of developmental lead exposure. When 30AB was from Russian 2b, lead significantly increased locomotor activity, whereas, when 30AB was from Oregon R, lead decreased it. 30AB contains about 125 genes among which are likely “candidate genes” for the observed lead-dependent behavioral changes.
Drosophila are thus a useful, underutilized model for studying behavioral, synaptic and genetic changes following chronic exposure to lead or other neurotoxins during development.</description><identifier>ISSN: 0161-813X</identifier><identifier>EISSN: 1872-9711</identifier><identifier>DOI: 10.1016/j.neuro.2009.01.004</identifier><identifier>PMID: 19428504</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Behavior ; Behavior, Animal - drug effects ; Behavior, Animal - physiology ; Biological and medical sciences ; Chemical and industrial products toxicology. Toxic occupational diseases ; Developmental lead exposure ; Developmental neurotoxicology ; Developmental plasticity ; Drosophila ; Drosophila melanogaster ; Drosophila melanogaster - drug effects ; Drosophila melanogaster - physiology ; Endocrine disruptor ; Genetic Variation - drug effects ; Genetic Variation - genetics ; Lead - administration & dosage ; Lead - toxicity ; Lead Poisoning - genetics ; Locomotor activity ; Male ; Medical sciences ; Metals and various inorganic compounds ; Motor Activity - drug effects ; Motor Activity - physiology ; Neurotoxin ; Quantitative Trait Loci - drug effects ; Quantitative Trait Loci - physiology ; Quantitative trait locus ; Toxicology</subject><ispartof>Neurotoxicology (Park Forest South), 2009-03, Vol.30 (2), p.305-311</ispartof><rights>2009 Elsevier Inc.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c519t-a75758b49520e6e438a4962af860bbaafe18761de96c33987e2a33b9d2d6cf903</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.neuro.2009.01.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21309904$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19428504$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hirsch, Helmut V.B.</creatorcontrib><creatorcontrib>Possidente, Debra</creatorcontrib><creatorcontrib>Averill, Sarah</creatorcontrib><creatorcontrib>Despain, Tamira Palmetto</creatorcontrib><creatorcontrib>Buytkins, Joel</creatorcontrib><creatorcontrib>Thomas, Valerie</creatorcontrib><creatorcontrib>Goebel, W. Paul</creatorcontrib><creatorcontrib>Shipp-Hilts, Asante</creatorcontrib><creatorcontrib>Wilson, Diane</creatorcontrib><creatorcontrib>Hollocher, Kurt</creatorcontrib><creatorcontrib>Possidente, Bernard</creatorcontrib><creatorcontrib>Lnenicka, Greg</creatorcontrib><creatorcontrib>Ruden, Douglas M.</creatorcontrib><title>Variations at a quantitative trait locus (QTL) affect development of behavior in lead-exposed Drosophila melanogaster</title><title>Neurotoxicology (Park Forest South)</title><addtitle>Neurotoxicology</addtitle><description>We developed
Drosophila melanogaster as a model to study correlated behavioral, neuronal and genetic effects of the neurotoxin lead, known to affect cognitive and behavioral development in children. We showed that, as in vertebrates, lead affects both synaptic development and complex behaviors (courtship, fecundity, locomotor activity) in
Drosophila. By assessing differential behavioral responses to developmental lead exposure among recombinant inbred
Drosophila lines (RI), derived from parental lines Oregon R and Russian 2b, we have now identified a genotype by environment interaction (GEI) for a behavioral trait affected by lead. Drosophila Activity Monitors (TriKinetics, Waltham, MA), which measure activity by counting the number of times a single fly in a small glass tube walks through an infrared beam aimed at the middle of the tube, were used to measure activity of flies, reared from eggs to 4 days of adult age on either control or lead-contaminated medium, from each of 75 RI lines. We observed a significant statistical association between the effect of lead on Average Daytime Activity (ADA) across lines and one marker locus, 30AB, on chromosome 2; we define this as a Quantitative Trait Locus (QTL) associated with behavioral effects of developmental lead exposure. When 30AB was from Russian 2b, lead significantly increased locomotor activity, whereas, when 30AB was from Oregon R, lead decreased it. 30AB contains about 125 genes among which are likely “candidate genes” for the observed lead-dependent behavioral changes.
Drosophila are thus a useful, underutilized model for studying behavioral, synaptic and genetic changes following chronic exposure to lead or other neurotoxins during development.</description><subject>Animals</subject><subject>Behavior</subject><subject>Behavior, Animal - drug effects</subject><subject>Behavior, Animal - physiology</subject><subject>Biological and medical sciences</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Developmental lead exposure</subject><subject>Developmental neurotoxicology</subject><subject>Developmental plasticity</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - drug effects</subject><subject>Drosophila melanogaster - physiology</subject><subject>Endocrine disruptor</subject><subject>Genetic Variation - drug effects</subject><subject>Genetic Variation - genetics</subject><subject>Lead - administration & dosage</subject><subject>Lead - toxicity</subject><subject>Lead Poisoning - genetics</subject><subject>Locomotor activity</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metals and various inorganic compounds</subject><subject>Motor Activity - drug effects</subject><subject>Motor Activity - physiology</subject><subject>Neurotoxin</subject><subject>Quantitative Trait Loci - drug effects</subject><subject>Quantitative Trait Loci - physiology</subject><subject>Quantitative trait locus</subject><subject>Toxicology</subject><issn>0161-813X</issn><issn>1872-9711</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAUhS0EotPCL0BC3oDKIsGPvLwoEipPaSSEVBA768a56XiUxKntRPDvcTujAhtWluzvHp9zDyHPOMs549XrfT7h4l0uGFM54zljxQOy4U0tMlVz_pBsEsWzhssfJ-Q0hD1jvKwr9ZiccFWIpmTFhizfwVuI1k2BQqRAbxaYoo3pakUaPdhIB2eWQM-_Xm1fUeh7NJF2uOLg5hGnSF1PW9zBap2ndqIDQpfhz9kF7Og774Kbd3YAOuIAk7uGENE_IY96GAI-PZ5n5NuH91eXn7Ltl4-fL99uM1NyFTOoy7ps2kKVgmGFhWygUJWAvqlY2wL0mNJWvENVGSlVU6MAKVvVia4yvWLyjLw56M5LO2Jnkl0Pg569HcH_0g6s_vdlsjt97VYta1k0ZZMEXh4FvLtZMEQ92mBwSFHQLUELVhRScJVAeQBNShw89vefcKZv69J7fVeXvq1LM65TXWnq-d_-_swc-0nAiyMAwcDQe5iMDfec4JIpdcddHDhM21wteh2MxclgZ33qS3fO_tfIb0ent5I</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Hirsch, Helmut V.B.</creator><creator>Possidente, Debra</creator><creator>Averill, Sarah</creator><creator>Despain, Tamira Palmetto</creator><creator>Buytkins, Joel</creator><creator>Thomas, Valerie</creator><creator>Goebel, W. Paul</creator><creator>Shipp-Hilts, Asante</creator><creator>Wilson, Diane</creator><creator>Hollocher, Kurt</creator><creator>Possidente, Bernard</creator><creator>Lnenicka, Greg</creator><creator>Ruden, Douglas M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20090301</creationdate><title>Variations at a quantitative trait locus (QTL) affect development of behavior in lead-exposed Drosophila melanogaster</title><author>Hirsch, Helmut V.B. ; Possidente, Debra ; Averill, Sarah ; Despain, Tamira Palmetto ; Buytkins, Joel ; Thomas, Valerie ; Goebel, W. 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Toxic occupational diseases</topic><topic>Developmental lead exposure</topic><topic>Developmental neurotoxicology</topic><topic>Developmental plasticity</topic><topic>Drosophila</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - drug effects</topic><topic>Drosophila melanogaster - physiology</topic><topic>Endocrine disruptor</topic><topic>Genetic Variation - drug effects</topic><topic>Genetic Variation - genetics</topic><topic>Lead - administration & dosage</topic><topic>Lead - toxicity</topic><topic>Lead Poisoning - genetics</topic><topic>Locomotor activity</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metals and various inorganic compounds</topic><topic>Motor Activity - drug effects</topic><topic>Motor Activity - physiology</topic><topic>Neurotoxin</topic><topic>Quantitative Trait Loci - drug effects</topic><topic>Quantitative Trait Loci - physiology</topic><topic>Quantitative trait locus</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hirsch, Helmut V.B.</creatorcontrib><creatorcontrib>Possidente, Debra</creatorcontrib><creatorcontrib>Averill, Sarah</creatorcontrib><creatorcontrib>Despain, Tamira Palmetto</creatorcontrib><creatorcontrib>Buytkins, Joel</creatorcontrib><creatorcontrib>Thomas, Valerie</creatorcontrib><creatorcontrib>Goebel, W. 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Paul</au><au>Shipp-Hilts, Asante</au><au>Wilson, Diane</au><au>Hollocher, Kurt</au><au>Possidente, Bernard</au><au>Lnenicka, Greg</au><au>Ruden, Douglas M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variations at a quantitative trait locus (QTL) affect development of behavior in lead-exposed Drosophila melanogaster</atitle><jtitle>Neurotoxicology (Park Forest South)</jtitle><addtitle>Neurotoxicology</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>30</volume><issue>2</issue><spage>305</spage><epage>311</epage><pages>305-311</pages><issn>0161-813X</issn><eissn>1872-9711</eissn><abstract>We developed
Drosophila melanogaster as a model to study correlated behavioral, neuronal and genetic effects of the neurotoxin lead, known to affect cognitive and behavioral development in children. We showed that, as in vertebrates, lead affects both synaptic development and complex behaviors (courtship, fecundity, locomotor activity) in
Drosophila. By assessing differential behavioral responses to developmental lead exposure among recombinant inbred
Drosophila lines (RI), derived from parental lines Oregon R and Russian 2b, we have now identified a genotype by environment interaction (GEI) for a behavioral trait affected by lead. Drosophila Activity Monitors (TriKinetics, Waltham, MA), which measure activity by counting the number of times a single fly in a small glass tube walks through an infrared beam aimed at the middle of the tube, were used to measure activity of flies, reared from eggs to 4 days of adult age on either control or lead-contaminated medium, from each of 75 RI lines. We observed a significant statistical association between the effect of lead on Average Daytime Activity (ADA) across lines and one marker locus, 30AB, on chromosome 2; we define this as a Quantitative Trait Locus (QTL) associated with behavioral effects of developmental lead exposure. When 30AB was from Russian 2b, lead significantly increased locomotor activity, whereas, when 30AB was from Oregon R, lead decreased it. 30AB contains about 125 genes among which are likely “candidate genes” for the observed lead-dependent behavioral changes.
Drosophila are thus a useful, underutilized model for studying behavioral, synaptic and genetic changes following chronic exposure to lead or other neurotoxins during development.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19428504</pmid><doi>10.1016/j.neuro.2009.01.004</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Behavior Behavior, Animal - drug effects Behavior, Animal - physiology Biological and medical sciences Chemical and industrial products toxicology. Toxic occupational diseases Developmental lead exposure Developmental neurotoxicology Developmental plasticity Drosophila Drosophila melanogaster Drosophila melanogaster - drug effects Drosophila melanogaster - physiology Endocrine disruptor Genetic Variation - drug effects Genetic Variation - genetics Lead - administration & dosage Lead - toxicity Lead Poisoning - genetics Locomotor activity Male Medical sciences Metals and various inorganic compounds Motor Activity - drug effects Motor Activity - physiology Neurotoxin Quantitative Trait Loci - drug effects Quantitative Trait Loci - physiology Quantitative trait locus Toxicology |
| title | Variations at a quantitative trait locus (QTL) affect development of behavior in lead-exposed Drosophila melanogaster |
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