Ptychography – a label free, high-contrast imaging technique for live cells using quantitative phase information
Cell imaging often relies on synthetic or genetic fluorescent labels, to provide contrast which can be far from ideal for imaging cells in their in vivo state. We report on the biological application of a, label-free, high contrast microscopy technique known as ptychography, in which the image produ...
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| Veröffentlicht in: | Scientific reports 2013-08, Vol.3 (1), p.2369-2369, Article 2369 |
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| creator | Marrison, Joanne Räty, Lotta Marriott, Poppy O'Toole, Peter |
| description | Cell imaging often relies on synthetic or genetic fluorescent labels, to provide contrast which can be far from ideal for imaging cells in their
in vivo
state. We report on the biological application of a, label-free, high contrast microscopy technique known as ptychography, in which the image producing step is transferred from the microscope lens to a high-speed phase retrieval algorithm. We demonstrate that this technology is appropriate for label-free imaging of adherent cells and is particularly suitable for reporting cellular changes such as mitosis, apoptosis and cell differentiation. The high contrast, artefact-free, focus-free information rich images allow dividing cells to be distinguished from non-dividing cells by a greater than two-fold increase in cell contrast and we demonstrate this technique is suitable for downstream automated cell segmentation and analysis. |
| doi_str_mv | 10.1038/srep02369 |
| format | Article |
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in vivo
state. We report on the biological application of a, label-free, high contrast microscopy technique known as ptychography, in which the image producing step is transferred from the microscope lens to a high-speed phase retrieval algorithm. We demonstrate that this technology is appropriate for label-free imaging of adherent cells and is particularly suitable for reporting cellular changes such as mitosis, apoptosis and cell differentiation. The high contrast, artefact-free, focus-free information rich images allow dividing cells to be distinguished from non-dividing cells by a greater than two-fold increase in cell contrast and we demonstrate this technique is suitable for downstream automated cell segmentation and analysis.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep02369</identifier><identifier>PMID: 23917865</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647 ; 631/1647/245 ; 631/1647/245/2226 ; Adherent cells ; Algorithms ; Animals ; Apoptosis ; Automation ; Cell cycle ; Cell differentiation ; Cell division ; Cells, Cultured - cytology ; Equipment Design ; Equipment Failure Analysis ; Humanities and Social Sciences ; Humans ; Image Enhancement - instrumentation ; Image processing ; Lighting - instrumentation ; Microscopy ; Microscopy, Phase-Contrast - instrumentation ; Mitosis ; multidisciplinary ; Reproducibility of Results ; Science ; Segmentation ; Sensitivity and Specificity ; Stem cells</subject><ispartof>Scientific reports, 2013-08, Vol.3 (1), p.2369-2369, Article 2369</ispartof><rights>The Author(s) 2013</rights><rights>Copyright Nature Publishing Group Aug 2013</rights><rights>Copyright © 2013, Macmillan Publishers Limited. All rights reserved 2013 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-9de4587cb9f6df217c8821dcbc605ff9a4c081c0d7090eca344c890633205bc33</citedby><cites>FETCH-LOGICAL-c504t-9de4587cb9f6df217c8821dcbc605ff9a4c081c0d7090eca344c890633205bc33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734479/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3734479/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,41101,42170,51557,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23917865$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marrison, Joanne</creatorcontrib><creatorcontrib>Räty, Lotta</creatorcontrib><creatorcontrib>Marriott, Poppy</creatorcontrib><creatorcontrib>O'Toole, Peter</creatorcontrib><title>Ptychography – a label free, high-contrast imaging technique for live cells using quantitative phase information</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Cell imaging often relies on synthetic or genetic fluorescent labels, to provide contrast which can be far from ideal for imaging cells in their
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Räty, Lotta ; Marriott, Poppy ; O'Toole, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-9de4587cb9f6df217c8821dcbc605ff9a4c081c0d7090eca344c890633205bc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>631/1647</topic><topic>631/1647/245</topic><topic>631/1647/245/2226</topic><topic>Adherent cells</topic><topic>Algorithms</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Automation</topic><topic>Cell cycle</topic><topic>Cell differentiation</topic><topic>Cell division</topic><topic>Cells, Cultured - cytology</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Image Enhancement - instrumentation</topic><topic>Image processing</topic><topic>Lighting - instrumentation</topic><topic>Microscopy</topic><topic>Microscopy, Phase-Contrast - instrumentation</topic><topic>Mitosis</topic><topic>multidisciplinary</topic><topic>Reproducibility of Results</topic><topic>Science</topic><topic>Segmentation</topic><topic>Sensitivity and Specificity</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marrison, Joanne</creatorcontrib><creatorcontrib>Räty, Lotta</creatorcontrib><creatorcontrib>Marriott, Poppy</creatorcontrib><creatorcontrib>O'Toole, Peter</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marrison, Joanne</au><au>Räty, Lotta</au><au>Marriott, Poppy</au><au>O'Toole, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ptychography – a label free, high-contrast imaging technique for live cells using quantitative phase information</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2013-08-06</date><risdate>2013</risdate><volume>3</volume><issue>1</issue><spage>2369</spage><epage>2369</epage><pages>2369-2369</pages><artnum>2369</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Cell imaging often relies on synthetic or genetic fluorescent labels, to provide contrast which can be far from ideal for imaging cells in their
in vivo
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| subjects | 631/1647 631/1647/245 631/1647/245/2226 Adherent cells Algorithms Animals Apoptosis Automation Cell cycle Cell differentiation Cell division Cells, Cultured - cytology Equipment Design Equipment Failure Analysis Humanities and Social Sciences Humans Image Enhancement - instrumentation Image processing Lighting - instrumentation Microscopy Microscopy, Phase-Contrast - instrumentation Mitosis multidisciplinary Reproducibility of Results Science Segmentation Sensitivity and Specificity Stem cells |
| title | Ptychography – a label free, high-contrast imaging technique for live cells using quantitative phase information |
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