Biological characteristics of CD133+ cells in nasopharyngeal carcinoma
Cancer stem cells are regarded as the cause of tumour formation and recurrence in nasopharyngeal carcinoma (NPC). However, ideal surface markers for stem cells in NPC remain unidentified. In the present study, we investigated the expression of CD133, Nanog and Sox2 in the nasopharyngeal carcinoma ce...
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| Veröffentlicht in: | Oncology reports 2013-07, Vol.30 (1), p.57-63 |
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| creator | ZHUANG, HUI-WEN MO, TING-TING HOU, WEI-JIAN XIONG, GUAN-XIA ZHU, XIAO-LIN FU, QING-LING WEN, WEI-PING |
| description | Cancer stem cells are regarded as the cause of tumour formation and recurrence in nasopharyngeal carcinoma (NPC). However, ideal surface markers for stem cells in NPC remain unidentified. In the present study, we investigated the expression of CD133, Nanog and Sox2 in the nasopharyngeal carcinoma cell line CNE2 and primarily cultured NPC cells using immunofluorescence or flow cytometry. A cell population with a CD133+ phenotype was enriched using magnetic-activated cell sorting technology. We demonstrated that CD133+ cells exhibited a strong potential for self-renewal, proliferation and differentiation and a greater potential for in vivo tumour formation in nude mice compared to CD133− cells, although the percentage of CD133+ cells was small. However, the specific marker antigens Nanog and Sox2 were simultaneously expressed in normal cancer stem cells. Our results showed that CD133 can serve as a specific surface marker for nasopharyngeal cancer stem cells. |
| doi_str_mv | 10.3892/or.2013.2408 |
| format | Article |
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However, ideal surface markers for stem cells in NPC remain unidentified. In the present study, we investigated the expression of CD133, Nanog and Sox2 in the nasopharyngeal carcinoma cell line CNE2 and primarily cultured NPC cells using immunofluorescence or flow cytometry. A cell population with a CD133+ phenotype was enriched using magnetic-activated cell sorting technology. We demonstrated that CD133+ cells exhibited a strong potential for self-renewal, proliferation and differentiation and a greater potential for in vivo tumour formation in nude mice compared to CD133− cells, although the percentage of CD133+ cells was small. However, the specific marker antigens Nanog and Sox2 were simultaneously expressed in normal cancer stem cells. Our results showed that CD133 can serve as a specific surface marker for nasopharyngeal cancer stem cells.</description><identifier>ISSN: 1021-335X</identifier><identifier>EISSN: 1791-2431</identifier><identifier>DOI: 10.3892/or.2013.2408</identifier><identifier>PMID: 23604326</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>AC133 Antigen ; Animals ; Antigens ; Antigens, CD - genetics ; Antigens, CD - metabolism ; Biomarkers, Tumor - analysis ; Brain cancer ; cancer stem cells ; Carcinoma ; CD133 ; Cell culture ; Cell cycle ; Cell Differentiation ; Cell Line, Tumor ; Cell Proliferation ; Colorectal cancer ; Flow cytometry ; Glycoproteins - genetics ; Glycoproteins - metabolism ; Homeodomain Proteins - biosynthesis ; Humans ; Leukemia ; Liver cancer ; Male ; Medical research ; Mice ; Mice, Inbred BALB C ; Nanog Homeobox Protein ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms - genetics ; Nasopharyngeal Neoplasms - metabolism ; Neoplastic Stem Cells - metabolism ; Octamer Transcription Factor-3 - biosynthesis ; Pancreatic cancer ; Peptides - genetics ; Peptides - metabolism ; Prostate cancer ; SOXB1 Transcription Factors - biosynthesis ; Stem cells ; Studies ; Throat cancer ; Tumors</subject><ispartof>Oncology reports, 2013-07, Vol.30 (1), p.57-63</ispartof><rights>Copyright © 2013, Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2013</rights><rights>Copyright © 2013, Spandidos Publications 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-b75ed5b5a44286104a51573f7879b10fe00d942ae2385ec5e4a0d23219d32e333</citedby><cites>FETCH-LOGICAL-c443t-b75ed5b5a44286104a51573f7879b10fe00d942ae2385ec5e4a0d23219d32e333</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23604326$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ZHUANG, HUI-WEN</creatorcontrib><creatorcontrib>MO, TING-TING</creatorcontrib><creatorcontrib>HOU, WEI-JIAN</creatorcontrib><creatorcontrib>XIONG, GUAN-XIA</creatorcontrib><creatorcontrib>ZHU, XIAO-LIN</creatorcontrib><creatorcontrib>FU, QING-LING</creatorcontrib><creatorcontrib>WEN, WEI-PING</creatorcontrib><title>Biological characteristics of CD133+ cells in nasopharyngeal carcinoma</title><title>Oncology reports</title><addtitle>Oncol Rep</addtitle><description>Cancer stem cells are regarded as the cause of tumour formation and recurrence in nasopharyngeal carcinoma (NPC). However, ideal surface markers for stem cells in NPC remain unidentified. In the present study, we investigated the expression of CD133, Nanog and Sox2 in the nasopharyngeal carcinoma cell line CNE2 and primarily cultured NPC cells using immunofluorescence or flow cytometry. A cell population with a CD133+ phenotype was enriched using magnetic-activated cell sorting technology. We demonstrated that CD133+ cells exhibited a strong potential for self-renewal, proliferation and differentiation and a greater potential for in vivo tumour formation in nude mice compared to CD133− cells, although the percentage of CD133+ cells was small. However, the specific marker antigens Nanog and Sox2 were simultaneously expressed in normal cancer stem cells. Our results showed that CD133 can serve as a specific surface marker for nasopharyngeal cancer stem cells.</description><subject>AC133 Antigen</subject><subject>Animals</subject><subject>Antigens</subject><subject>Antigens, CD - genetics</subject><subject>Antigens, CD - metabolism</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Brain cancer</subject><subject>cancer stem cells</subject><subject>Carcinoma</subject><subject>CD133</subject><subject>Cell culture</subject><subject>Cell cycle</subject><subject>Cell Differentiation</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Colorectal cancer</subject><subject>Flow cytometry</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - metabolism</subject><subject>Homeodomain Proteins - biosynthesis</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Liver cancer</subject><subject>Male</subject><subject>Medical research</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nanog Homeobox Protein</subject><subject>Nasopharyngeal Carcinoma</subject><subject>Nasopharyngeal Neoplasms - genetics</subject><subject>Nasopharyngeal Neoplasms - metabolism</subject><subject>Neoplastic Stem Cells - metabolism</subject><subject>Octamer Transcription Factor-3 - biosynthesis</subject><subject>Pancreatic cancer</subject><subject>Peptides - genetics</subject><subject>Peptides - metabolism</subject><subject>Prostate cancer</subject><subject>SOXB1 Transcription Factors - biosynthesis</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Throat cancer</subject><subject>Tumors</subject><issn>1021-335X</issn><issn>1791-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkdGLEzEQxoMo3l31zedjQZAD3TqZSZrdF0GrVaHgi4JvIc1mezm2SS_ZCv73Zm09PZ9mYH4zfN98jD3jMKemxdcxzRE4zVFA84Cdc9XyGgXxh6UH5DWR_H7GLnK-AUAFi_YxO0NagCBcnLPVOx-HuPXWDJW9NsnY0SWfR29zFftq-Z4TvaysG4Zc-VAFk-O-YD_D1k0bJlkf4s48YY96M2T39FRn7Nvqw9flp3r95ePn5dt1bYWgsd4o6Tq5kUYIbBYchJFcKupVo9oNh94BdK1A45Aa6ax0wkCHhLztCB0Rzdib4939YbNznXVhTGbQ--R3RZSOxuv7k-Cv9Tb-0KSwxaJhxq5OB1K8Pbg86p3Pkz0TXDxkzUkKVUiuCvr8P_QmHlIo9jRvy_OUxN_UqyNlU8w5uf5ODAc9BaRj0lNAegqo4Jf_GriD_yRSgBdHIO9N6HwX8193qSaogdcA5Wm_ANXjltk</recordid><startdate>20130701</startdate><enddate>20130701</enddate><creator>ZHUANG, HUI-WEN</creator><creator>MO, TING-TING</creator><creator>HOU, WEI-JIAN</creator><creator>XIONG, GUAN-XIA</creator><creator>ZHU, XIAO-LIN</creator><creator>FU, QING-LING</creator><creator>WEN, WEI-PING</creator><general>D.A. Spandidos</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130701</creationdate><title>Biological characteristics of CD133+ cells in nasopharyngeal carcinoma</title><author>ZHUANG, HUI-WEN ; MO, TING-TING ; HOU, WEI-JIAN ; XIONG, GUAN-XIA ; ZHU, XIAO-LIN ; FU, QING-LING ; WEN, WEI-PING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-b75ed5b5a44286104a51573f7879b10fe00d942ae2385ec5e4a0d23219d32e333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>AC133 Antigen</topic><topic>Animals</topic><topic>Antigens</topic><topic>Antigens, CD - genetics</topic><topic>Antigens, CD - metabolism</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Brain cancer</topic><topic>cancer stem cells</topic><topic>Carcinoma</topic><topic>CD133</topic><topic>Cell culture</topic><topic>Cell cycle</topic><topic>Cell Differentiation</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Colorectal cancer</topic><topic>Flow cytometry</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - metabolism</topic><topic>Homeodomain Proteins - biosynthesis</topic><topic>Humans</topic><topic>Leukemia</topic><topic>Liver cancer</topic><topic>Male</topic><topic>Medical research</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nanog Homeobox Protein</topic><topic>Nasopharyngeal Carcinoma</topic><topic>Nasopharyngeal Neoplasms - genetics</topic><topic>Nasopharyngeal Neoplasms - metabolism</topic><topic>Neoplastic Stem Cells - metabolism</topic><topic>Octamer Transcription Factor-3 - biosynthesis</topic><topic>Pancreatic cancer</topic><topic>Peptides - genetics</topic><topic>Peptides - metabolism</topic><topic>Prostate cancer</topic><topic>SOXB1 Transcription Factors - biosynthesis</topic><topic>Stem cells</topic><topic>Studies</topic><topic>Throat cancer</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ZHUANG, HUI-WEN</creatorcontrib><creatorcontrib>MO, TING-TING</creatorcontrib><creatorcontrib>HOU, WEI-JIAN</creatorcontrib><creatorcontrib>XIONG, GUAN-XIA</creatorcontrib><creatorcontrib>ZHU, XIAO-LIN</creatorcontrib><creatorcontrib>FU, QING-LING</creatorcontrib><creatorcontrib>WEN, WEI-PING</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ZHUANG, HUI-WEN</au><au>MO, TING-TING</au><au>HOU, WEI-JIAN</au><au>XIONG, GUAN-XIA</au><au>ZHU, XIAO-LIN</au><au>FU, QING-LING</au><au>WEN, WEI-PING</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biological characteristics of CD133+ cells in nasopharyngeal carcinoma</atitle><jtitle>Oncology reports</jtitle><addtitle>Oncol Rep</addtitle><date>2013-07-01</date><risdate>2013</risdate><volume>30</volume><issue>1</issue><spage>57</spage><epage>63</epage><pages>57-63</pages><issn>1021-335X</issn><eissn>1791-2431</eissn><abstract>Cancer stem cells are regarded as the cause of tumour formation and recurrence in nasopharyngeal carcinoma (NPC). However, ideal surface markers for stem cells in NPC remain unidentified. In the present study, we investigated the expression of CD133, Nanog and Sox2 in the nasopharyngeal carcinoma cell line CNE2 and primarily cultured NPC cells using immunofluorescence or flow cytometry. A cell population with a CD133+ phenotype was enriched using magnetic-activated cell sorting technology. We demonstrated that CD133+ cells exhibited a strong potential for self-renewal, proliferation and differentiation and a greater potential for in vivo tumour formation in nude mice compared to CD133− cells, although the percentage of CD133+ cells was small. However, the specific marker antigens Nanog and Sox2 were simultaneously expressed in normal cancer stem cells. Our results showed that CD133 can serve as a specific surface marker for nasopharyngeal cancer stem cells.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>23604326</pmid><doi>10.3892/or.2013.2408</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | AC133 Antigen Animals Antigens Antigens, CD - genetics Antigens, CD - metabolism Biomarkers, Tumor - analysis Brain cancer cancer stem cells Carcinoma CD133 Cell culture Cell cycle Cell Differentiation Cell Line, Tumor Cell Proliferation Colorectal cancer Flow cytometry Glycoproteins - genetics Glycoproteins - metabolism Homeodomain Proteins - biosynthesis Humans Leukemia Liver cancer Male Medical research Mice Mice, Inbred BALB C Nanog Homeobox Protein Nasopharyngeal Carcinoma Nasopharyngeal Neoplasms - genetics Nasopharyngeal Neoplasms - metabolism Neoplastic Stem Cells - metabolism Octamer Transcription Factor-3 - biosynthesis Pancreatic cancer Peptides - genetics Peptides - metabolism Prostate cancer SOXB1 Transcription Factors - biosynthesis Stem cells Studies Throat cancer Tumors |
| title | Biological characteristics of CD133+ cells in nasopharyngeal carcinoma |
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