Effect of nitric oxide on anterior segment physiology in monkeys
To determine the effect of the nitric oxide donor, sodium nitroprusside (SNP), and the nitric oxide synthase (NOS) inhibitor, L-nitro-arginine-methylester (L-NAME), on IOP, mean arterial pressure (MAP), pupil diameter (PD), refraction (Rfx), aqueous humor formation (AHF), and outflow facility (OF) i...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 2013-07, Vol.54 (7), p.5103-5110 |
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| creator | Heyne, Galen W Kiland, Julie A Kaufman, Paul L Gabelt, B'Ann T |
| description | To determine the effect of the nitric oxide donor, sodium nitroprusside (SNP), and the nitric oxide synthase (NOS) inhibitor, L-nitro-arginine-methylester (L-NAME), on IOP, mean arterial pressure (MAP), pupil diameter (PD), refraction (Rfx), aqueous humor formation (AHF), and outflow facility (OF) in monkeys.
Monkeys were treated with single or multiple topical treatments of 500 μg SNP or L-NAME to one eye. IOP was determined by Goldmann applanation tonometry, PD with vernier calipers in room light, Rfx by Hartinger coincidence refractometry, AHF by fluorophotometry, and MAP with a blood pressure monitor. OF was determined by two-level constant pressure perfusion following anterior chamber exchange.
Following four topical treatments with 500 μg SNP, 30 minutes apart, IOP was significantly decreased from 2 to 6 hours compared with the contralateral control with the maximum IOP reduction of 20% at 3 hours (P < 0.001). PD, Rfx, and AHF were unchanged. Effects on MAP were variable. OF after SNP exchange was significantly increased by 77% (P < 0.05) at 10(-3) M. Topical L-NAME had no effect on IOP, PD, Rfx, or MAP.
Enhancement of nitric oxide concentration at targeted tissues in the anterior segment may be a useful approach for IOP reduction for glaucoma therapy. Additional studies are warranted before conclusions can be made regarding the effect of NOS inhibition on ocular physiology in nonhuman primates. |
| doi_str_mv | 10.1167/iovs.12-11491 |
| format | Article |
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Monkeys were treated with single or multiple topical treatments of 500 μg SNP or L-NAME to one eye. IOP was determined by Goldmann applanation tonometry, PD with vernier calipers in room light, Rfx by Hartinger coincidence refractometry, AHF by fluorophotometry, and MAP with a blood pressure monitor. OF was determined by two-level constant pressure perfusion following anterior chamber exchange.
Following four topical treatments with 500 μg SNP, 30 minutes apart, IOP was significantly decreased from 2 to 6 hours compared with the contralateral control with the maximum IOP reduction of 20% at 3 hours (P < 0.001). PD, Rfx, and AHF were unchanged. Effects on MAP were variable. OF after SNP exchange was significantly increased by 77% (P < 0.05) at 10(-3) M. Topical L-NAME had no effect on IOP, PD, Rfx, or MAP.
Enhancement of nitric oxide concentration at targeted tissues in the anterior segment may be a useful approach for IOP reduction for glaucoma therapy. Additional studies are warranted before conclusions can be made regarding the effect of NOS inhibition on ocular physiology in nonhuman primates.</description><identifier>ISSN: 1552-5783</identifier><identifier>ISSN: 0146-0404</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.12-11491</identifier><identifier>PMID: 23800771</identifier><language>eng</language><publisher>United States: The Association for Research in Vision and Ophthalmology</publisher><subject>Analysis of Variance ; Animals ; Anterior Eye Segment - drug effects ; Anterior Eye Segment - physiology ; Aqueous Humor - drug effects ; Blood Pressure - drug effects ; Dose-Response Relationship, Drug ; Enzyme Inhibitors - pharmacology ; Heart Rate - drug effects ; Intraocular Pressure - drug effects ; Macaca fascicularis - physiology ; NG-Nitroarginine Methyl Ester - pharmacology ; Nitric Oxide - antagonists & inhibitors ; Nitric Oxide - physiology ; Nitric Oxide Donors - pharmacology ; Nitroprusside - pharmacology ; Pupil - drug effects ; Refraction, Ocular - drug effects</subject><ispartof>Investigative ophthalmology & visual science, 2013-07, Vol.54 (7), p.5103-5110</ispartof><rights>Copyright 2013 The Association for Research in Vision and Ophthalmology, Inc. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-c237ba475432cbc00902bf3ced76a23fafa96acde0f1b448c5a9b720413a6dc23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3729238/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3729238/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23800771$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heyne, Galen W</creatorcontrib><creatorcontrib>Kiland, Julie A</creatorcontrib><creatorcontrib>Kaufman, Paul L</creatorcontrib><creatorcontrib>Gabelt, B'Ann T</creatorcontrib><title>Effect of nitric oxide on anterior segment physiology in monkeys</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>To determine the effect of the nitric oxide donor, sodium nitroprusside (SNP), and the nitric oxide synthase (NOS) inhibitor, L-nitro-arginine-methylester (L-NAME), on IOP, mean arterial pressure (MAP), pupil diameter (PD), refraction (Rfx), aqueous humor formation (AHF), and outflow facility (OF) in monkeys.
Monkeys were treated with single or multiple topical treatments of 500 μg SNP or L-NAME to one eye. IOP was determined by Goldmann applanation tonometry, PD with vernier calipers in room light, Rfx by Hartinger coincidence refractometry, AHF by fluorophotometry, and MAP with a blood pressure monitor. OF was determined by two-level constant pressure perfusion following anterior chamber exchange.
Following four topical treatments with 500 μg SNP, 30 minutes apart, IOP was significantly decreased from 2 to 6 hours compared with the contralateral control with the maximum IOP reduction of 20% at 3 hours (P < 0.001). PD, Rfx, and AHF were unchanged. Effects on MAP were variable. OF after SNP exchange was significantly increased by 77% (P < 0.05) at 10(-3) M. Topical L-NAME had no effect on IOP, PD, Rfx, or MAP.
Enhancement of nitric oxide concentration at targeted tissues in the anterior segment may be a useful approach for IOP reduction for glaucoma therapy. Additional studies are warranted before conclusions can be made regarding the effect of NOS inhibition on ocular physiology in nonhuman primates.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Anterior Eye Segment - drug effects</subject><subject>Anterior Eye Segment - physiology</subject><subject>Aqueous Humor - drug effects</subject><subject>Blood Pressure - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Heart Rate - drug effects</subject><subject>Intraocular Pressure - drug effects</subject><subject>Macaca fascicularis - physiology</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>Nitric Oxide - antagonists & inhibitors</subject><subject>Nitric Oxide - physiology</subject><subject>Nitric Oxide Donors - pharmacology</subject><subject>Nitroprusside - pharmacology</subject><subject>Pupil - drug effects</subject><subject>Refraction, Ocular - drug effects</subject><issn>1552-5783</issn><issn>0146-0404</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1OwzAQhC0EoqVw5Ir8AileO4mTCwJV5UeqxAXOluPYqaGxKztU5O2bUqjKaVfamdndD6FrIFOAnN9av4lToAlAWsIJGkOW0STjBTs96kfoIsYPQigAJedoRFlBCOcwRvdzY7TqsDfY2S5Yhf23rTX2DkvX6WB9wFE3rXYdXi_7aP3KNz22Drfefeo-XqIzI1dRX_3WCXp_nL_NnpPF69PL7GGRKFbwLlGU8UqmPEsZVZUipCS0MkzpmueSMiONLHOpak0MVGlaqEyWFackBSbzenBP0N0-d_1VtbpWw0FBrsQ62FaGXnhpxf-Js0vR-I1gnJbDv0NAsg9QwccYtDl4gYgdSrFDKYCKH5SD_uZ44UH9x45tAaC2ckE</recordid><startdate>20130730</startdate><enddate>20130730</enddate><creator>Heyne, Galen W</creator><creator>Kiland, Julie A</creator><creator>Kaufman, Paul L</creator><creator>Gabelt, B'Ann T</creator><general>The Association for Research in Vision and Ophthalmology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20130730</creationdate><title>Effect of nitric oxide on anterior segment physiology in monkeys</title><author>Heyne, Galen W ; Kiland, Julie A ; Kaufman, Paul L ; Gabelt, B'Ann T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-c237ba475432cbc00902bf3ced76a23fafa96acde0f1b448c5a9b720413a6dc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Anterior Eye Segment - drug effects</topic><topic>Anterior Eye Segment - physiology</topic><topic>Aqueous Humor - drug effects</topic><topic>Blood Pressure - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Heart Rate - drug effects</topic><topic>Intraocular Pressure - drug effects</topic><topic>Macaca fascicularis - physiology</topic><topic>NG-Nitroarginine Methyl Ester - pharmacology</topic><topic>Nitric Oxide - antagonists & inhibitors</topic><topic>Nitric Oxide - physiology</topic><topic>Nitric Oxide Donors - pharmacology</topic><topic>Nitroprusside - pharmacology</topic><topic>Pupil - drug effects</topic><topic>Refraction, Ocular - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heyne, Galen W</creatorcontrib><creatorcontrib>Kiland, Julie A</creatorcontrib><creatorcontrib>Kaufman, Paul L</creatorcontrib><creatorcontrib>Gabelt, B'Ann T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heyne, Galen W</au><au>Kiland, Julie A</au><au>Kaufman, Paul L</au><au>Gabelt, B'Ann T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of nitric oxide on anterior segment physiology in monkeys</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2013-07-30</date><risdate>2013</risdate><volume>54</volume><issue>7</issue><spage>5103</spage><epage>5110</epage><pages>5103-5110</pages><issn>1552-5783</issn><issn>0146-0404</issn><eissn>1552-5783</eissn><abstract>To determine the effect of the nitric oxide donor, sodium nitroprusside (SNP), and the nitric oxide synthase (NOS) inhibitor, L-nitro-arginine-methylester (L-NAME), on IOP, mean arterial pressure (MAP), pupil diameter (PD), refraction (Rfx), aqueous humor formation (AHF), and outflow facility (OF) in monkeys.
Monkeys were treated with single or multiple topical treatments of 500 μg SNP or L-NAME to one eye. IOP was determined by Goldmann applanation tonometry, PD with vernier calipers in room light, Rfx by Hartinger coincidence refractometry, AHF by fluorophotometry, and MAP with a blood pressure monitor. OF was determined by two-level constant pressure perfusion following anterior chamber exchange.
Following four topical treatments with 500 μg SNP, 30 minutes apart, IOP was significantly decreased from 2 to 6 hours compared with the contralateral control with the maximum IOP reduction of 20% at 3 hours (P < 0.001). PD, Rfx, and AHF were unchanged. Effects on MAP were variable. OF after SNP exchange was significantly increased by 77% (P < 0.05) at 10(-3) M. Topical L-NAME had no effect on IOP, PD, Rfx, or MAP.
Enhancement of nitric oxide concentration at targeted tissues in the anterior segment may be a useful approach for IOP reduction for glaucoma therapy. Additional studies are warranted before conclusions can be made regarding the effect of NOS inhibition on ocular physiology in nonhuman primates.</abstract><cop>United States</cop><pub>The Association for Research in Vision and Ophthalmology</pub><pmid>23800771</pmid><doi>10.1167/iovs.12-11491</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Analysis of Variance Animals Anterior Eye Segment - drug effects Anterior Eye Segment - physiology Aqueous Humor - drug effects Blood Pressure - drug effects Dose-Response Relationship, Drug Enzyme Inhibitors - pharmacology Heart Rate - drug effects Intraocular Pressure - drug effects Macaca fascicularis - physiology NG-Nitroarginine Methyl Ester - pharmacology Nitric Oxide - antagonists & inhibitors Nitric Oxide - physiology Nitric Oxide Donors - pharmacology Nitroprusside - pharmacology Pupil - drug effects Refraction, Ocular - drug effects |
| title | Effect of nitric oxide on anterior segment physiology in monkeys |
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