Repeated vitrification/warming of human sperm gives better results than repeated slow programmable freezing
In this study, we compared the effects of repeated freezing/thawing of human sperm by our in-house method of rapid freezing with slow programmable freezing. Sperm samples from 11 normozoospermic subjects were processed through density gradients and divided into three aliquots： non-frozen, rapid free...
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| Veröffentlicht in: | Asian journal of andrology 2012-11, Vol.14 (6), p.850-854 |
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| creator | Vutyavanich, Teraporn Lattiwongsakorn, Worashorn Piromlertamorn, Waraporn Samchimchom, Sudarat |
| description | In this study, we compared the effects of repeated freezing/thawing of human sperm by our in-house method of rapid freezing with slow programmable freezing. Sperm samples from 11 normozoospermic subjects were processed through density gradients and divided into three aliquots： non-frozen, rapid freezing and slow programmable freezing. Sperm in the rapid freezing group had better motility and viability than those in the slow freezing group （P〈O.01） after the first, second and third cycles of freezing/thawing, but there was no difference in morphology. In the second experiment, rapid freezing was repeated three times in 20 subjects. The samples from each thawing cycle were evaluated for DNA fragmentation using the alkaline comet assay. DNA fragmentation began to increase considerably after the second cycle of freezing/thawing, but to a level that was not clinically important. In the third experiment, rapid freezing was done repeatedly in 10 subjects, until no motile sperm were observed after thawing. The median number of repeated freezing/thawing that yielded no motile sperm was seven （range： 5-8, mean： 6.8）. In conclusion, we demonstrated that repeated freezing/thawing of processed semen using our rapid freezing method gave better results than standard slow programmable freezing. This method can help maximize the usage of precious cryopreserved sperm samples in assisted reproduction technology. |
| doi_str_mv | 10.1038/aja.2012.106 |
| format | Article |
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Sperm samples from 11 normozoospermic subjects were processed through density gradients and divided into three aliquots： non-frozen, rapid freezing and slow programmable freezing. Sperm in the rapid freezing group had better motility and viability than those in the slow freezing group （P〈O.01） after the first, second and third cycles of freezing/thawing, but there was no difference in morphology. In the second experiment, rapid freezing was repeated three times in 20 subjects. The samples from each thawing cycle were evaluated for DNA fragmentation using the alkaline comet assay. DNA fragmentation began to increase considerably after the second cycle of freezing/thawing, but to a level that was not clinically important. In the third experiment, rapid freezing was done repeatedly in 10 subjects, until no motile sperm were observed after thawing. The median number of repeated freezing/thawing that yielded no motile sperm was seven （range： 5-8, mean： 6.8）. In conclusion, we demonstrated that repeated freezing/thawing of processed semen using our rapid freezing method gave better results than standard slow programmable freezing. This method can help maximize the usage of precious cryopreserved sperm samples in assisted reproduction technology.</description><identifier>ISSN: 1008-682X</identifier><identifier>EISSN: 1745-7262</identifier><identifier>DOI: 10.1038/aja.2012.106</identifier><identifier>PMID: 23064685</identifier><language>eng</language><publisher>China: Medknow Publications & Media Pvt. Ltd</publisher><subject>Adult ; Cryopreservation - methods ; DNA Fragmentation ; DNA片段化 ; Female ; Freezing ; Humans ; Male ; Original ; Semen Preservation - methods ; Sperm Motility ; Spermatozoa - drug effects ; Vitrification ; 人类 ; 冷冻精子 ; 冻结 ; 可编程 ; 快速冷冻法 ; 气候变暖 ; 玻璃化冷冻</subject><ispartof>Asian journal of andrology, 2012-11, Vol.14 (6), p.850-854</ispartof><rights>Copyright Nature Publishing Group Nov 2012</rights><rights>Copyright © 2012 SIMM & SJTU 2012 SIMM & SJTU</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-66947192780f7c573f89612c52d29a6472901db1512b85d313e7fe962b569513</citedby><cites>FETCH-LOGICAL-c438t-66947192780f7c573f89612c52d29a6472901db1512b85d313e7fe962b569513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84127X/84127X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720100/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3720100/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23064685$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vutyavanich, Teraporn</creatorcontrib><creatorcontrib>Lattiwongsakorn, Worashorn</creatorcontrib><creatorcontrib>Piromlertamorn, Waraporn</creatorcontrib><creatorcontrib>Samchimchom, Sudarat</creatorcontrib><title>Repeated vitrification/warming of human sperm gives better results than repeated slow programmable freezing</title><title>Asian journal of andrology</title><addtitle>Asian Journal of Andrology</addtitle><description>In this study, we compared the effects of repeated freezing/thawing of human sperm by our in-house method of rapid freezing with slow programmable freezing. Sperm samples from 11 normozoospermic subjects were processed through density gradients and divided into three aliquots： non-frozen, rapid freezing and slow programmable freezing. Sperm in the rapid freezing group had better motility and viability than those in the slow freezing group （P〈O.01） after the first, second and third cycles of freezing/thawing, but there was no difference in morphology. In the second experiment, rapid freezing was repeated three times in 20 subjects. The samples from each thawing cycle were evaluated for DNA fragmentation using the alkaline comet assay. DNA fragmentation began to increase considerably after the second cycle of freezing/thawing, but to a level that was not clinically important. In the third experiment, rapid freezing was done repeatedly in 10 subjects, until no motile sperm were observed after thawing. The median number of repeated freezing/thawing that yielded no motile sperm was seven （range： 5-8, mean： 6.8）. In conclusion, we demonstrated that repeated freezing/thawing of processed semen using our rapid freezing method gave better results than standard slow programmable freezing. This method can help maximize the usage of precious cryopreserved sperm samples in assisted reproduction technology.</description><subject>Adult</subject><subject>Cryopreservation - methods</subject><subject>DNA Fragmentation</subject><subject>DNA片段化</subject><subject>Female</subject><subject>Freezing</subject><subject>Humans</subject><subject>Male</subject><subject>Original</subject><subject>Semen Preservation - methods</subject><subject>Sperm Motility</subject><subject>Spermatozoa - drug effects</subject><subject>Vitrification</subject><subject>人类</subject><subject>冷冻精子</subject><subject>冻结</subject><subject>可编程</subject><subject>快速冷冻法</subject><subject>气候变暖</subject><subject>玻璃化冷冻</subject><issn>1008-682X</issn><issn>1745-7262</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkU1r3DAQhkVpaNK0t56LSi891Im-LV0KJfQLAoGQQ29CtsdebW3LkeQN7a-vQjZLm5NmmIcHzbwIvaHkjBKuz93WnTFCWenUM3RCayGrmin2vNSE6Epp9vMYvUxpSwjj1JgX6JhxooTS8gT9uoYFXIYO73yOvvetyz7M53cuTn4ecOjxZp3cjNMCccKD30HCDeQMEUdI65gTzpsyj4-eNIY7vMQwRDdNrhkB9xHgT5G9Qke9GxO83r-n6Obrl5uL79Xl1bcfF58vq1ZwnSuljKipYbUmfd3KmvfaKMpayTpmnBI1M4R2DZWUNVp2nHKoezCKNVIZSfkp-vSgXdZmgq6FOUc32iX6ycXfNjhv_5_MfmOHsLO8LnckpAg-7AUx3K6Qsp18amEc3QxhTZZSwQ2RXIqCvn-CbsMa57JdobjmjBAhC_XxgWpjSClCf_gMJfY-RFtCtPchlk4V_O2_Cxzgx9QK8G7v24R5uC2nPTBCMG4YEfwv1G2j-Q</recordid><startdate>20121101</startdate><enddate>20121101</enddate><creator>Vutyavanich, Teraporn</creator><creator>Lattiwongsakorn, Worashorn</creator><creator>Piromlertamorn, Waraporn</creator><creator>Samchimchom, Sudarat</creator><general>Medknow Publications & Media Pvt. 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Sperm samples from 11 normozoospermic subjects were processed through density gradients and divided into three aliquots： non-frozen, rapid freezing and slow programmable freezing. Sperm in the rapid freezing group had better motility and viability than those in the slow freezing group （P〈O.01） after the first, second and third cycles of freezing/thawing, but there was no difference in morphology. In the second experiment, rapid freezing was repeated three times in 20 subjects. The samples from each thawing cycle were evaluated for DNA fragmentation using the alkaline comet assay. DNA fragmentation began to increase considerably after the second cycle of freezing/thawing, but to a level that was not clinically important. In the third experiment, rapid freezing was done repeatedly in 10 subjects, until no motile sperm were observed after thawing. The median number of repeated freezing/thawing that yielded no motile sperm was seven （range： 5-8, mean： 6.8）. In conclusion, we demonstrated that repeated freezing/thawing of processed semen using our rapid freezing method gave better results than standard slow programmable freezing. This method can help maximize the usage of precious cryopreserved sperm samples in assisted reproduction technology.</abstract><cop>China</cop><pub>Medknow Publications & Media Pvt. Ltd</pub><pmid>23064685</pmid><doi>10.1038/aja.2012.106</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1008-682X |
| ispartof | Asian journal of andrology, 2012-11, Vol.14 (6), p.850-854 |
| issn | 1008-682X 1745-7262 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adult Cryopreservation - methods DNA Fragmentation DNA片段化 Female Freezing Humans Male Original Semen Preservation - methods Sperm Motility Spermatozoa - drug effects Vitrification 人类 冷冻精子 冻结 可编程 快速冷冻法 气候变暖 玻璃化冷冻 |
| title | Repeated vitrification/warming of human sperm gives better results than repeated slow programmable freezing |
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