Gene Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds of Descurainia sophia, a Potential Anticancer Drug
Descurainia sophia has been traditionally used in Korean medicine for treatment of diverse diseases and their symptoms, such as cough, asthma, and edema. Our previous results showed that ethanol extract of the seeds of D. sophia (EEDS) has a potent cytotoxic effect on human cancer cells. In this stu...
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| description | Descurainia sophia has been traditionally used in Korean medicine for treatment of diverse diseases and their symptoms, such as cough, asthma, and edema. Our previous results showed that ethanol extract of the seeds of D. sophia (EEDS) has a potent cytotoxic effect on human cancer cells. In this study, we reveal the molecular events that are induced by EEDS treatment in A549 human lung cancer cells. The dose-dependent effect of EEDS on gene expression was measured via a microarray analysis. Gene ontology and pathway analyses were performed to identify functional involvement of genes regulated by EEDS. From gene expression analyses, two major dose-dependent patterns were observed after EEDS treatment. One pattern consisted of 1,680 downregulated genes primarily involved in metabolic processes (FDR < 0.01). The second pattern consisted of 1,673 upregulated genes primarily involved in signaling processes (FDR < 0.01). Pathway activity analyses revealed that the metabolism-related pathways and signaling-related pathways were regulated by the EEDS in dose-dependent and reciprocal manners. In conclusion, the identified biphasic regulatory mechanism involving activation of signaling pathways may provide molecular evidence to explain the inhibitory effect of EEDS on A549 cell growth. |
| doi_str_mv | 10.1155/2013/584604 |
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Our previous results showed that ethanol extract of the seeds of D. sophia (EEDS) has a potent cytotoxic effect on human cancer cells. In this study, we reveal the molecular events that are induced by EEDS treatment in A549 human lung cancer cells. The dose-dependent effect of EEDS on gene expression was measured via a microarray analysis. Gene ontology and pathway analyses were performed to identify functional involvement of genes regulated by EEDS. From gene expression analyses, two major dose-dependent patterns were observed after EEDS treatment. One pattern consisted of 1,680 downregulated genes primarily involved in metabolic processes (FDR < 0.01). The second pattern consisted of 1,673 upregulated genes primarily involved in signaling processes (FDR < 0.01). Pathway activity analyses revealed that the metabolism-related pathways and signaling-related pathways were regulated by the EEDS in dose-dependent and reciprocal manners. In conclusion, the identified biphasic regulatory mechanism involving activation of signaling pathways may provide molecular evidence to explain the inhibitory effect of EEDS on A549 cell growth.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2013/584604</identifier><identifier>PMID: 23935669</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Alternative medicine ; Apoptosis ; Asthma ; Bioinformatics ; Cancer therapies ; Cell cycle ; Cell growth ; Chemotherapy ; Cough ; Cytotoxicity ; Descurainia sophia ; DNA microarrays ; Edema ; Ethanol ; Gene expression ; Herbal medicine ; Kinases ; Lung cancer ; Lung carcinoma ; Medical prognosis ; Medical research ; Medical treatment ; Metabolism ; Non-small cell lung carcinoma ; Seeds ; Signal transduction ; Small cell lung carcinoma</subject><ispartof>Evidence-based complementary and alternative medicine, 2013-01, Vol.2013 (2013), p.1-13</ispartof><rights>Copyright © 2013 Bu-Yeo Kim et al.</rights><rights>Copyright © 2013 Bu-Yeo Kim et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2013 Bu-Yeo Kim et al. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-6b39e4ac02ec73d7c16028ce886850c303dd0e0855efe49a611dd11d863250a33</citedby><cites>FETCH-LOGICAL-c462t-6b39e4ac02ec73d7c16028ce886850c303dd0e0855efe49a611dd11d863250a33</cites><orcidid>0000-0002-5756-5470 ; 0000-0001-5949-2407 ; 0000-0003-1437-708X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712200/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712200/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23935669$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Jang, Bo-Hyoung</contributor><creatorcontrib>Kim, Bu-Yeo</creatorcontrib><creatorcontrib>Lee, Jun</creatorcontrib><creatorcontrib>Park, Sung Joon</creatorcontrib><creatorcontrib>Bang, Ok-Sun</creatorcontrib><creatorcontrib>Kim, No Soo</creatorcontrib><title>Gene Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds of Descurainia sophia, a Potential Anticancer Drug</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Descurainia sophia has been traditionally used in Korean medicine for treatment of diverse diseases and their symptoms, such as cough, asthma, and edema. Our previous results showed that ethanol extract of the seeds of D. sophia (EEDS) has a potent cytotoxic effect on human cancer cells. In this study, we reveal the molecular events that are induced by EEDS treatment in A549 human lung cancer cells. The dose-dependent effect of EEDS on gene expression was measured via a microarray analysis. Gene ontology and pathway analyses were performed to identify functional involvement of genes regulated by EEDS. From gene expression analyses, two major dose-dependent patterns were observed after EEDS treatment. One pattern consisted of 1,680 downregulated genes primarily involved in metabolic processes (FDR < 0.01). The second pattern consisted of 1,673 upregulated genes primarily involved in signaling processes (FDR < 0.01). Pathway activity analyses revealed that the metabolism-related pathways and signaling-related pathways were regulated by the EEDS in dose-dependent and reciprocal manners. In conclusion, the identified biphasic regulatory mechanism involving activation of signaling pathways may provide molecular evidence to explain the inhibitory effect of EEDS on A549 cell growth.</description><subject>Alternative medicine</subject><subject>Apoptosis</subject><subject>Asthma</subject><subject>Bioinformatics</subject><subject>Cancer therapies</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Chemotherapy</subject><subject>Cough</subject><subject>Cytotoxicity</subject><subject>Descurainia sophia</subject><subject>DNA microarrays</subject><subject>Edema</subject><subject>Ethanol</subject><subject>Gene expression</subject><subject>Herbal medicine</subject><subject>Kinases</subject><subject>Lung cancer</subject><subject>Lung carcinoma</subject><subject>Medical prognosis</subject><subject>Medical research</subject><subject>Medical treatment</subject><subject>Metabolism</subject><subject>Non-small cell lung 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Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds of Descurainia sophia, a Potential Anticancer Drug</title><author>Kim, Bu-Yeo ; Lee, Jun ; Park, Sung Joon ; Bang, Ok-Sun ; Kim, No Soo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-6b39e4ac02ec73d7c16028ce886850c303dd0e0855efe49a611dd11d863250a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Alternative medicine</topic><topic>Apoptosis</topic><topic>Asthma</topic><topic>Bioinformatics</topic><topic>Cancer therapies</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Chemotherapy</topic><topic>Cough</topic><topic>Cytotoxicity</topic><topic>Descurainia sophia</topic><topic>DNA microarrays</topic><topic>Edema</topic><topic>Ethanol</topic><topic>Gene expression</topic><topic>Herbal medicine</topic><topic>Kinases</topic><topic>Lung 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Carcinoma Cell Line following Treatment with the Seeds of Descurainia sophia, a Potential Anticancer Drug</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>2013</volume><issue>2013</issue><spage>1</spage><epage>13</epage><pages>1-13</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Descurainia sophia has been traditionally used in Korean medicine for treatment of diverse diseases and their symptoms, such as cough, asthma, and edema. Our previous results showed that ethanol extract of the seeds of D. sophia (EEDS) has a potent cytotoxic effect on human cancer cells. In this study, we reveal the molecular events that are induced by EEDS treatment in A549 human lung cancer cells. The dose-dependent effect of EEDS on gene expression was measured via a microarray analysis. Gene ontology and pathway analyses were performed to identify functional involvement of genes regulated by EEDS. From gene expression analyses, two major dose-dependent patterns were observed after EEDS treatment. One pattern consisted of 1,680 downregulated genes primarily involved in metabolic processes (FDR < 0.01). The second pattern consisted of 1,673 upregulated genes primarily involved in signaling processes (FDR < 0.01). Pathway activity analyses revealed that the metabolism-related pathways and signaling-related pathways were regulated by the EEDS in dose-dependent and reciprocal manners. 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| subjects | Alternative medicine Apoptosis Asthma Bioinformatics Cancer therapies Cell cycle Cell growth Chemotherapy Cough Cytotoxicity Descurainia sophia DNA microarrays Edema Ethanol Gene expression Herbal medicine Kinases Lung cancer Lung carcinoma Medical prognosis Medical research Medical treatment Metabolism Non-small cell lung carcinoma Seeds Signal transduction Small cell lung carcinoma |
| title | Gene Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds of Descurainia sophia, a Potential Anticancer Drug |
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