Simultaneous and sensitive detection of human immunodeficiency virus type 1 (HIV) drug resistant genotypes by multiplex oligonucleotide ligation assay

•Oligonucleotide ligation assay (OLA) detects point mutations in HIV-1.•It is a simple and cost-effective method for use in resource-limited settings.•Here OLA is adapted to detect simultaneously two HIV drug resistance mutations.•Multiplexed OLA reliably detects minority genotypes when compared to...

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Veröffentlicht in:	Journal of virological methods 2013-09, Vol.192 (1-2), p.39-43
Hauptverfasser:	Ellis, Giovanina M., Vlaskin, Tatyana A., Koth, Andrew, Vaz, Louise E., Dross, Sandra E., Beck, Ingrid A., Frenkel, Lisa M.
Format:	Artikel
Sprache:	eng
Schlagworte:	antiretroviral agents Antiretroviral drug resistance codons cost effectiveness drug resistance drugs Genotype HIV HIV infections HIV Infections - drug therapy HIV Infections - virology HIV-1 - genetics HIV-1 - isolation & purification Human immunodeficiency virus 1 Humans Microbial Sensitivity Tests - methods Minority genotypes Molecular Diagnostic Techniques - methods mutants Oligonucleotide ligation assay Oligonucleotide Probes - genetics oligonucleotides plasmids Point Mutation polymerase chain reaction screening Sensitivity and Specificity
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