Neutrophil activation by Campylobacter concisus
Campylobacter concisus is an emerging enteric pathogen associated with prolonged diarrhoea and possibly inflammatory bowel disease in children as well as adults, but the interaction with cells of the innate immune system is unclear. The magnitude of systemic immunoglobulin response in acute infectio...
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| Veröffentlicht in: | Gut pathogens 2013-07, Vol.5 (1), p.17-17, Article 17 |
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| creator | Sørensen, Nina B Nielsen, Hans L Varming, Kim Nielsen, Henrik |
| description | Campylobacter concisus is an emerging enteric pathogen associated with prolonged diarrhoea and possibly inflammatory bowel disease in children as well as adults, but the interaction with cells of the innate immune system is unclear. The magnitude of systemic immunoglobulin response in acute infection is unknown.
Neutrophils from healthy volunteers were activated with five faecal isolates of C. concisus from patients with gastroenteritis as well as the oral reference strain C. concisus ATCC33237. Neutrophils were tested for the expression of adherence molecule CD11b by immunoflourescence and for oxidative burst response by chemiluminescence. The opsonic activity in a chemiluminescence assay was assessed with heat treated serum from patients with C. concisus infection.
A strong and dose-dependent activation of neutrophil adherence molecule CD11b and oxidative burst response was demonstrated with all six C. concisus isolates. Bacteria opsonised in heat treated serum induced an increased chemiluminescence response. Heat treated serum from patients with C. concisus infection did not have a higher opsonic activity than heat treated serum from healthy volunteers.
C. concisus has the capability to activate the innate immune system by stimulating neutrophil cells to increased adherence molecule expression and oxidative burst response, both crucial for acute inflammation. In a chemiluminescence assay the opsonic activity of heat treated serum from patients was not increased compared to heat treated control serum suggesting a weak systemic IgG response to infection. |
| doi_str_mv | 10.1186/1757-4749-5-17 |
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Neutrophils from healthy volunteers were activated with five faecal isolates of C. concisus from patients with gastroenteritis as well as the oral reference strain C. concisus ATCC33237. Neutrophils were tested for the expression of adherence molecule CD11b by immunoflourescence and for oxidative burst response by chemiluminescence. The opsonic activity in a chemiluminescence assay was assessed with heat treated serum from patients with C. concisus infection.
A strong and dose-dependent activation of neutrophil adherence molecule CD11b and oxidative burst response was demonstrated with all six C. concisus isolates. Bacteria opsonised in heat treated serum induced an increased chemiluminescence response. Heat treated serum from patients with C. concisus infection did not have a higher opsonic activity than heat treated serum from healthy volunteers.
C. concisus has the capability to activate the innate immune system by stimulating neutrophil cells to increased adherence molecule expression and oxidative burst response, both crucial for acute inflammation. In a chemiluminescence assay the opsonic activity of heat treated serum from patients was not increased compared to heat treated control serum suggesting a weak systemic IgG response to infection.</description><identifier>ISSN: 1757-4749</identifier><identifier>EISSN: 1757-4749</identifier><identifier>DOI: 10.1186/1757-4749-5-17</identifier><identifier>PMID: 23819746</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Bacteria ; Campylobacter ; Campylobacter concisus ; Cellular control mechanisms ; Disease ; Diseases ; Experiments ; Immune system ; Immunoglobulins ; Medical equipment and supplies industry ; Medical research ; Medical test kit industry ; Medicine, Experimental ; Microorganisms ; Neutrophils ; Patients ; Ratios ; Statistical analysis ; United Kingdom ; United States ; Values</subject><ispartof>Gut pathogens, 2013-07, Vol.5 (1), p.17-17, Article 17</ispartof><rights>COPYRIGHT 2013 BioMed Central Ltd.</rights><rights>2013 Sørensen et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2013 Sørensen et al.; licensee BioMed Central Ltd. 2013 Sørensen et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b645t-9ef9096ed5612c384d29461ac26023c9f74550d0a80203e64305c9368c8c5a163</citedby><cites>FETCH-LOGICAL-b645t-9ef9096ed5612c384d29461ac26023c9f74550d0a80203e64305c9368c8c5a163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702423/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3702423/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23819746$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sørensen, Nina B</creatorcontrib><creatorcontrib>Nielsen, Hans L</creatorcontrib><creatorcontrib>Varming, Kim</creatorcontrib><creatorcontrib>Nielsen, Henrik</creatorcontrib><title>Neutrophil activation by Campylobacter concisus</title><title>Gut pathogens</title><addtitle>Gut Pathog</addtitle><description>Campylobacter concisus is an emerging enteric pathogen associated with prolonged diarrhoea and possibly inflammatory bowel disease in children as well as adults, but the interaction with cells of the innate immune system is unclear. The magnitude of systemic immunoglobulin response in acute infection is unknown.
Neutrophils from healthy volunteers were activated with five faecal isolates of C. concisus from patients with gastroenteritis as well as the oral reference strain C. concisus ATCC33237. Neutrophils were tested for the expression of adherence molecule CD11b by immunoflourescence and for oxidative burst response by chemiluminescence. The opsonic activity in a chemiluminescence assay was assessed with heat treated serum from patients with C. concisus infection.
A strong and dose-dependent activation of neutrophil adherence molecule CD11b and oxidative burst response was demonstrated with all six C. concisus isolates. Bacteria opsonised in heat treated serum induced an increased chemiluminescence response. Heat treated serum from patients with C. concisus infection did not have a higher opsonic activity than heat treated serum from healthy volunteers.
C. concisus has the capability to activate the innate immune system by stimulating neutrophil cells to increased adherence molecule expression and oxidative burst response, both crucial for acute inflammation. In a chemiluminescence assay the opsonic activity of heat treated serum from patients was not increased compared to heat treated control serum suggesting a weak systemic IgG response to infection.</description><subject>Bacteria</subject><subject>Campylobacter</subject><subject>Campylobacter concisus</subject><subject>Cellular control mechanisms</subject><subject>Disease</subject><subject>Diseases</subject><subject>Experiments</subject><subject>Immune system</subject><subject>Immunoglobulins</subject><subject>Medical equipment and supplies industry</subject><subject>Medical research</subject><subject>Medical test kit industry</subject><subject>Medicine, Experimental</subject><subject>Microorganisms</subject><subject>Neutrophils</subject><subject>Patients</subject><subject>Ratios</subject><subject>Statistical analysis</subject><subject>United Kingdom</subject><subject>United States</subject><subject>Values</subject><issn>1757-4749</issn><issn>1757-4749</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqFksuLFDEQxoMo7kOvHmVAkL30bt6PizAMvmDRi55DOp2eyZJOxqR7Yf570-w6zuiI5JCi6lcflS8FwCsErxGS_AYJJhoqqGpYg8QTcL5PPD2Iz8BFKXcQckolew7OMJFICcrPwc0XN405bTc-LIwd_b0ZfYqLdrdYmWG7C6mtWZcXNkXry1RegGe9CcW9fLwvwfcP77-tPjW3Xz9-Xi1vm5ZTNjbK9Qoq7jrGEbZE0g4rypGxmENMrOoFZQx20EiIIXGcEsisIlxaaZlBnFyCdw-626kdXGddHLMJepv9YPJOJ-P1cSX6jV6ne00ExBSTKrB8EGh9-ofAccWmQc-G6dkwzWpcNa4eh8jpx-TKqAdfrAvBRJemohFFmCHBCfs_SpSkhBKGK_rmD_QuTTlWN2dKISkkF7-ptQlO-9inOqWdRfWSESqkgEJWqjlBrV109Ukput7X9BF_fYKvp3ODtycb3h40bJwJ46akMM1bUk4q25xKya7fW42gnlf1b3NfH_7wHv-1m-Qn3ibf1g</recordid><startdate>20130703</startdate><enddate>20130703</enddate><creator>Sørensen, Nina B</creator><creator>Nielsen, Hans L</creator><creator>Varming, Kim</creator><creator>Nielsen, Henrik</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope><scope>5PM</scope></search><sort><creationdate>20130703</creationdate><title>Neutrophil activation by Campylobacter concisus</title><author>Sørensen, Nina B ; Nielsen, Hans L ; Varming, Kim ; Nielsen, Henrik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b645t-9ef9096ed5612c384d29461ac26023c9f74550d0a80203e64305c9368c8c5a163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Bacteria</topic><topic>Campylobacter</topic><topic>Campylobacter concisus</topic><topic>Cellular control mechanisms</topic><topic>Disease</topic><topic>Diseases</topic><topic>Experiments</topic><topic>Immune system</topic><topic>Immunoglobulins</topic><topic>Medical equipment and supplies industry</topic><topic>Medical research</topic><topic>Medical test kit industry</topic><topic>Medicine, Experimental</topic><topic>Microorganisms</topic><topic>Neutrophils</topic><topic>Patients</topic><topic>Ratios</topic><topic>Statistical analysis</topic><topic>United Kingdom</topic><topic>United States</topic><topic>Values</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sørensen, Nina B</creatorcontrib><creatorcontrib>Nielsen, Hans L</creatorcontrib><creatorcontrib>Varming, Kim</creatorcontrib><creatorcontrib>Nielsen, Henrik</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Gut pathogens</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sørensen, Nina B</au><au>Nielsen, Hans L</au><au>Varming, Kim</au><au>Nielsen, Henrik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutrophil activation by Campylobacter concisus</atitle><jtitle>Gut pathogens</jtitle><addtitle>Gut Pathog</addtitle><date>2013-07-03</date><risdate>2013</risdate><volume>5</volume><issue>1</issue><spage>17</spage><epage>17</epage><pages>17-17</pages><artnum>17</artnum><issn>1757-4749</issn><eissn>1757-4749</eissn><abstract>Campylobacter concisus is an emerging enteric pathogen associated with prolonged diarrhoea and possibly inflammatory bowel disease in children as well as adults, but the interaction with cells of the innate immune system is unclear. The magnitude of systemic immunoglobulin response in acute infection is unknown.
Neutrophils from healthy volunteers were activated with five faecal isolates of C. concisus from patients with gastroenteritis as well as the oral reference strain C. concisus ATCC33237. Neutrophils were tested for the expression of adherence molecule CD11b by immunoflourescence and for oxidative burst response by chemiluminescence. The opsonic activity in a chemiluminescence assay was assessed with heat treated serum from patients with C. concisus infection.
A strong and dose-dependent activation of neutrophil adherence molecule CD11b and oxidative burst response was demonstrated with all six C. concisus isolates. Bacteria opsonised in heat treated serum induced an increased chemiluminescence response. Heat treated serum from patients with C. concisus infection did not have a higher opsonic activity than heat treated serum from healthy volunteers.
C. concisus has the capability to activate the innate immune system by stimulating neutrophil cells to increased adherence molecule expression and oxidative burst response, both crucial for acute inflammation. In a chemiluminescence assay the opsonic activity of heat treated serum from patients was not increased compared to heat treated control serum suggesting a weak systemic IgG response to infection.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>23819746</pmid><doi>10.1186/1757-4749-5-17</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; SpringerNature Journals; PubMed Central Open Access; Springer Nature OA Free Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Bacteria Campylobacter Campylobacter concisus Cellular control mechanisms Disease Diseases Experiments Immune system Immunoglobulins Medical equipment and supplies industry Medical research Medical test kit industry Medicine, Experimental Microorganisms Neutrophils Patients Ratios Statistical analysis United Kingdom United States Values |
| title | Neutrophil activation by Campylobacter concisus |
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