Droplet microfluidics for amplification-free genetic detection of single cells
In this article we present a novel droplet microfluidic chip enabling amplification-free detection of single pathogenic cells. The device streamlines multiple functionalities to carry out sample digitization, cell lysis, probe-target hybridization for subsequent fluorescent detection. A peptide nucl...
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| Veröffentlicht in: | Lab on a chip 2012-09, Vol.12 (18), p.3341-3347 |
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| container_title | Lab on a chip |
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| creator | Rane, Tushar D Zec, Helena C Puleo, Chris Lee, Abraham P Wang, Tza-Huei |
| description | In this article we present a novel droplet microfluidic chip enabling amplification-free detection of single pathogenic cells. The device streamlines multiple functionalities to carry out sample digitization, cell lysis, probe-target hybridization for subsequent fluorescent detection. A peptide nucleic acid fluorescence resonance energy transfer probe (PNA beacon) is used to detect 16S rRNA present in pathogenic cells. Initially the sensitivity and quantification abilities of the platform are tested using a synthetic target mimicking the actual expression level of 16S rRNA in single cells. The capability of the device to perform "sample-to-answer" pathogen detection of single cells is demonstrated using E. coli as a model pathogen. |
| doi_str_mv | 10.1039/c2lc40537g |
| format | Article |
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| identifier | ISSN: 1473-0197 |
| ispartof | Lab on a chip, 2012-09, Vol.12 (18), p.3341-3347 |
| issn | 1473-0197 1473-0189 |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals; Alma/SFX Local Collection |
| subjects | Colias Escherichia coli - isolation & purification Escherichia coli - metabolism Fluorescence Resonance Energy Transfer Microfluidics - instrumentation Microfluidics - methods Nucleic Acid Hybridization Peptide Nucleic Acids - chemistry Peptide Nucleic Acids - metabolism RNA, Ribosomal, 16S - metabolism |
| title | Droplet microfluidics for amplification-free genetic detection of single cells |
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