Calretinin immunohistochemistry: A new cost-effective and easy method for diagnosis of Hirschsprung's disease
To evaluate the efficacy of calretinin immunostaining in diagnosing Hirschsprung's disease (HD). Sixty cases were studied over a period of 1 year (July 2010-June 2011). There were 36 full-thickness biopsies and 24 resected specimens. Calretinin processing was done on the paraffin-embedded block...
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| Veröffentlicht in: | Journal of Indian Association of Pediatric Surgeons 2013-04, Vol.18 (2), p.66-68 |
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| container_title | Journal of Indian Association of Pediatric Surgeons |
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| creator | Kannaiyan, Lavanya Madabhushi, Sujani Malleboyina, Ramani Are, Narendra Kumar Reddy, K Ramesh Rao, Bhuvaneshwar |
| description | To evaluate the efficacy of calretinin immunostaining in diagnosing Hirschsprung's disease (HD).
Sixty cases were studied over a period of 1 year (July 2010-June 2011). There were 36 full-thickness biopsies and 24 resected specimens. Calretinin processing was done on the paraffin-embedded blocks after routine histopathological examination.
Of the 36 biopsy specimens, in 19 cases HD was diagnosed by hematoxylin and eosin (H and E) staining earlier. In 2 patients, ganglion cells were seen and HD was ruled out. In 15 cases, there was a diagnostic dilemma and calretinin was used. Ganglion cells were found in 3 specimens and nerve fibers in 5. In all 24 resected specimens, calretinin correlated with the findings on H and E staining.
Calretinin was extremely useful in solving the suspicious and indeterminate cases of HD. It can serve as a valuable cost-effective diagnostic aid in the centers where acetylcholinesterase enzyme histochemistry is not available. |
| doi_str_mv | 10.4103/0971-9261.109355 |
| format | Article |
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Sixty cases were studied over a period of 1 year (July 2010-June 2011). There were 36 full-thickness biopsies and 24 resected specimens. Calretinin processing was done on the paraffin-embedded blocks after routine histopathological examination.
Of the 36 biopsy specimens, in 19 cases HD was diagnosed by hematoxylin and eosin (H and E) staining earlier. In 2 patients, ganglion cells were seen and HD was ruled out. In 15 cases, there was a diagnostic dilemma and calretinin was used. Ganglion cells were found in 3 specimens and nerve fibers in 5. In all 24 resected specimens, calretinin correlated with the findings on H and E staining.
Calretinin was extremely useful in solving the suspicious and indeterminate cases of HD. It can serve as a valuable cost-effective diagnostic aid in the centers where acetylcholinesterase enzyme histochemistry is not available.</description><identifier>ISSN: 0971-9261</identifier><identifier>EISSN: 1998-3891</identifier><identifier>DOI: 10.4103/0971-9261.109355</identifier><identifier>PMID: 23798809</identifier><language>eng</language><publisher>India: Medknow Publications and Media Pvt. Ltd</publisher><subject>Analysis ; Children & youth ; Diagnostic tests ; Gastrointestinal diseases ; Immunohistochemistry ; Medical diagnosis ; Methods ; Original</subject><ispartof>Journal of Indian Association of Pediatric Surgeons, 2013-04, Vol.18 (2), p.66-68</ispartof><rights>COPYRIGHT 2013 Medknow Publications and Media Pvt. Ltd.</rights><rights>Copyright Medknow Publications & Media Pvt Ltd Apr 2013</rights><rights>Copyright: © Journal of Indian Association of Pediatric Surgeons 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3687149/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3687149/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23798809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kannaiyan, Lavanya</creatorcontrib><creatorcontrib>Madabhushi, Sujani</creatorcontrib><creatorcontrib>Malleboyina, Ramani</creatorcontrib><creatorcontrib>Are, Narendra Kumar</creatorcontrib><creatorcontrib>Reddy, K Ramesh</creatorcontrib><creatorcontrib>Rao, Bhuvaneshwar</creatorcontrib><title>Calretinin immunohistochemistry: A new cost-effective and easy method for diagnosis of Hirschsprung's disease</title><title>Journal of Indian Association of Pediatric Surgeons</title><addtitle>J Indian Assoc Pediatr Surg</addtitle><description>To evaluate the efficacy of calretinin immunostaining in diagnosing Hirschsprung's disease (HD).
Sixty cases were studied over a period of 1 year (July 2010-June 2011). There were 36 full-thickness biopsies and 24 resected specimens. Calretinin processing was done on the paraffin-embedded blocks after routine histopathological examination.
Of the 36 biopsy specimens, in 19 cases HD was diagnosed by hematoxylin and eosin (H and E) staining earlier. In 2 patients, ganglion cells were seen and HD was ruled out. In 15 cases, there was a diagnostic dilemma and calretinin was used. Ganglion cells were found in 3 specimens and nerve fibers in 5. In all 24 resected specimens, calretinin correlated with the findings on H and E staining.
Calretinin was extremely useful in solving the suspicious and indeterminate cases of HD. It can serve as a valuable cost-effective diagnostic aid in the centers where acetylcholinesterase enzyme histochemistry is not available.</description><subject>Analysis</subject><subject>Children & youth</subject><subject>Diagnostic tests</subject><subject>Gastrointestinal diseases</subject><subject>Immunohistochemistry</subject><subject>Medical diagnosis</subject><subject>Methods</subject><subject>Original</subject><issn>0971-9261</issn><issn>1998-3891</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptksFvFCEUxonR2LV692RIPNjLrLyBGcCDyWZTrUkTL3qesMxjlmYGVpip2f9eGmu1ZsPhJXw_vvf4gJDXwNYCGH_PtIRK1y2sgWneNE_ICrRWFVcanpLVg3xGXuR8wxgXrIHn5KzmUivF9IpMWzMmnH3wgfppWkLc-zxHu8ep1HT8QDc04E9qY54rdA7t7G-RmtBTNPlIJ5z3sacuJtp7M4SYfabR0Sufst3nQ1rC8C4XLRccX5JnzowZX93Xc_L90-W37VV1_fXzl-3muho4iLmSQopaWWeN7Y3lSjbKouK8qbEBNL2RtWp3jXY7yQxI0MK0YA1TyjXQKuDn5ONv38Oym7C3GOZkxu6Q_GTSsYvGd4-V4PfdEG873ioJQheDi3uDFH8smOeuxGFxHE3AuOQOuKyZlG0tCvr2P_QmLimU6xWqFi2TGuRfajAjdj64WPraO9Nuw-uGayHhbu7qBDVgwDJkDOh82X7Er0_wZfXl-ezJA2_-DeYhkT8fgv8CZ-a3nA</recordid><startdate>201304</startdate><enddate>201304</enddate><creator>Kannaiyan, Lavanya</creator><creator>Madabhushi, Sujani</creator><creator>Malleboyina, Ramani</creator><creator>Are, Narendra Kumar</creator><creator>Reddy, K Ramesh</creator><creator>Rao, Bhuvaneshwar</creator><general>Medknow Publications and Media Pvt. 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Sixty cases were studied over a period of 1 year (July 2010-June 2011). There were 36 full-thickness biopsies and 24 resected specimens. Calretinin processing was done on the paraffin-embedded blocks after routine histopathological examination.
Of the 36 biopsy specimens, in 19 cases HD was diagnosed by hematoxylin and eosin (H and E) staining earlier. In 2 patients, ganglion cells were seen and HD was ruled out. In 15 cases, there was a diagnostic dilemma and calretinin was used. Ganglion cells were found in 3 specimens and nerve fibers in 5. In all 24 resected specimens, calretinin correlated with the findings on H and E staining.
Calretinin was extremely useful in solving the suspicious and indeterminate cases of HD. It can serve as a valuable cost-effective diagnostic aid in the centers where acetylcholinesterase enzyme histochemistry is not available.</abstract><cop>India</cop><pub>Medknow Publications and Media Pvt. Ltd</pub><pmid>23798809</pmid><doi>10.4103/0971-9261.109355</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Children & youth Diagnostic tests Gastrointestinal diseases Immunohistochemistry Medical diagnosis Methods Original |
| title | Calretinin immunohistochemistry: A new cost-effective and easy method for diagnosis of Hirschsprung's disease |
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