Dengue-specific subviral nanoparticles: design, creation and characterization
Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially usefu...
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| Veröffentlicht in: | Journal of nanobiotechnology 2013-05, Vol.11 (1), p.15-15, Article 15 |
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| creator | Khetarpal, Niyati Poddar, Ankur Nemani, Satish K Dhar, Nisha Patil, Aravind Negi, Priyanka Perween, Ashiya Viswanathan, Ramaswamy Lünsdorf, Heinrich Tyagi, Poornima Raut, Rajendra Arora, Upasana Jain, Swatantra K Rinas, Ursula Swaminathan, Sathyamangalam Khanna, Navin |
| description | Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.
We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.
The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.
VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications. |
| doi_str_mv | 10.1186/1477-3155-11-15 |
| format | Article |
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We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.
The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.
VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.</description><identifier>ISSN: 1477-3155</identifier><identifier>EISSN: 1477-3155</identifier><identifier>DOI: 10.1186/1477-3155-11-15</identifier><identifier>PMID: 23706089</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Accessibility ; Animals ; Antibodies ; Antigens ; Antigens, Viral - isolation & purification ; Antigens, Viral - ultrastructure ; Biomedical engineering ; Biotechnology ; Cell Extracts ; Cercopithecus aethiops ; Dengue ; Dengue - diagnosis ; Dengue - virology ; Dengue fever ; Dengue virus ; Dengue Virus - physiology ; Dengue viruses ; Disease ; Genetic engineering ; Hepatitis ; Hepatitis B virus ; Medical research ; Medicine, Experimental ; Microbiology ; Nanocomposites ; Nanomaterials ; Nanoparticles ; Nanoparticles - chemistry ; Nanostructure ; Physiological aspects ; Pichia - metabolism ; Platforms ; Protein Structure, Tertiary ; Proteins ; Recombinant Fusion Proteins - isolation & purification ; Scholarships & fellowships ; Software ; Species Specificity ; Strategy ; Vaccines ; Vero Cells ; Viral antibodies ; Viral Envelope Proteins ; Viral Proteins - chemistry ; Viral Proteins - isolation & purification ; Virion - metabolism</subject><ispartof>Journal of nanobiotechnology, 2013-05, Vol.11 (1), p.15-15, Article 15</ispartof><rights>COPYRIGHT 2013 BioMed Central Ltd.</rights><rights>2013 Khetarpal et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2013 Khetarpal et al.; licensee BioMed Central Ltd. 2013 Khetarpal et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c588t-6aeca9e6bfc91f0cea3dc293adec03274f1cd17ca8725992f134dc7995bfe07c3</citedby><cites>FETCH-LOGICAL-c588t-6aeca9e6bfc91f0cea3dc293adec03274f1cd17ca8725992f134dc7995bfe07c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680219/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680219/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23706089$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khetarpal, Niyati</creatorcontrib><creatorcontrib>Poddar, Ankur</creatorcontrib><creatorcontrib>Nemani, Satish K</creatorcontrib><creatorcontrib>Dhar, Nisha</creatorcontrib><creatorcontrib>Patil, Aravind</creatorcontrib><creatorcontrib>Negi, Priyanka</creatorcontrib><creatorcontrib>Perween, Ashiya</creatorcontrib><creatorcontrib>Viswanathan, Ramaswamy</creatorcontrib><creatorcontrib>Lünsdorf, Heinrich</creatorcontrib><creatorcontrib>Tyagi, Poornima</creatorcontrib><creatorcontrib>Raut, Rajendra</creatorcontrib><creatorcontrib>Arora, Upasana</creatorcontrib><creatorcontrib>Jain, Swatantra K</creatorcontrib><creatorcontrib>Rinas, Ursula</creatorcontrib><creatorcontrib>Swaminathan, Sathyamangalam</creatorcontrib><creatorcontrib>Khanna, Navin</creatorcontrib><title>Dengue-specific subviral nanoparticles: design, creation and characterization</title><title>Journal of nanobiotechnology</title><addtitle>J Nanobiotechnology</addtitle><description>Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.
We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.
The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.
VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.</description><subject>Accessibility</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Antigens, Viral - isolation & purification</subject><subject>Antigens, Viral - ultrastructure</subject><subject>Biomedical engineering</subject><subject>Biotechnology</subject><subject>Cell Extracts</subject><subject>Cercopithecus aethiops</subject><subject>Dengue</subject><subject>Dengue - diagnosis</subject><subject>Dengue - virology</subject><subject>Dengue fever</subject><subject>Dengue virus</subject><subject>Dengue Virus - physiology</subject><subject>Dengue viruses</subject><subject>Disease</subject><subject>Genetic engineering</subject><subject>Hepatitis</subject><subject>Hepatitis B virus</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Microbiology</subject><subject>Nanocomposites</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Nanostructure</subject><subject>Physiological aspects</subject><subject>Pichia - metabolism</subject><subject>Platforms</subject><subject>Protein Structure, Tertiary</subject><subject>Proteins</subject><subject>Recombinant Fusion Proteins - isolation & purification</subject><subject>Scholarships & fellowships</subject><subject>Software</subject><subject>Species Specificity</subject><subject>Strategy</subject><subject>Vaccines</subject><subject>Vero Cells</subject><subject>Viral antibodies</subject><subject>Viral Envelope Proteins</subject><subject>Viral Proteins - chemistry</subject><subject>Viral Proteins - isolation & purification</subject><subject>Virion - 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subviral nanoparticles: design, creation and characterization</title><author>Khetarpal, Niyati ; Poddar, Ankur ; Nemani, Satish K ; Dhar, Nisha ; Patil, Aravind ; Negi, Priyanka ; Perween, Ashiya ; Viswanathan, Ramaswamy ; Lünsdorf, Heinrich ; Tyagi, Poornima ; Raut, Rajendra ; Arora, Upasana ; Jain, Swatantra K ; Rinas, Ursula ; Swaminathan, Sathyamangalam ; Khanna, Navin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c588t-6aeca9e6bfc91f0cea3dc293adec03274f1cd17ca8725992f134dc7995bfe07c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Accessibility</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Antigens, Viral - isolation & purification</topic><topic>Antigens, Viral - ultrastructure</topic><topic>Biomedical engineering</topic><topic>Biotechnology</topic><topic>Cell Extracts</topic><topic>Cercopithecus aethiops</topic><topic>Dengue</topic><topic>Dengue - diagnosis</topic><topic>Dengue - virology</topic><topic>Dengue fever</topic><topic>Dengue virus</topic><topic>Dengue Virus - physiology</topic><topic>Dengue viruses</topic><topic>Disease</topic><topic>Genetic engineering</topic><topic>Hepatitis</topic><topic>Hepatitis B virus</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>Microbiology</topic><topic>Nanocomposites</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Nanostructure</topic><topic>Physiological aspects</topic><topic>Pichia - metabolism</topic><topic>Platforms</topic><topic>Protein Structure, Tertiary</topic><topic>Proteins</topic><topic>Recombinant Fusion Proteins - isolation & purification</topic><topic>Scholarships & fellowships</topic><topic>Software</topic><topic>Species Specificity</topic><topic>Strategy</topic><topic>Vaccines</topic><topic>Vero Cells</topic><topic>Viral 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Navin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dengue-specific subviral nanoparticles: design, creation and characterization</atitle><jtitle>Journal of nanobiotechnology</jtitle><addtitle>J Nanobiotechnology</addtitle><date>2013-05-25</date><risdate>2013</risdate><volume>11</volume><issue>1</issue><spage>15</spage><epage>15</epage><pages>15-15</pages><artnum>15</artnum><issn>1477-3155</issn><eissn>1477-3155</eissn><abstract>Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.
We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.
The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.
VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>23706089</pmid><doi>10.1186/1477-3155-11-15</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of nanobiotechnology, 2013-05, Vol.11 (1), p.15-15, Article 15 |
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| source | PubMed (Medline); SpringerOpen; MEDLINE; Full-Text Journals in Chemistry (Open access); DOAJ Directory of Open Access Journals; SpringerLink (Online service); EZB Electronic Journals Library; PubMed Central Open Access |
| subjects | Accessibility Animals Antibodies Antigens Antigens, Viral - isolation & purification Antigens, Viral - ultrastructure Biomedical engineering Biotechnology Cell Extracts Cercopithecus aethiops Dengue Dengue - diagnosis Dengue - virology Dengue fever Dengue virus Dengue Virus - physiology Dengue viruses Disease Genetic engineering Hepatitis Hepatitis B virus Medical research Medicine, Experimental Microbiology Nanocomposites Nanomaterials Nanoparticles Nanoparticles - chemistry Nanostructure Physiological aspects Pichia - metabolism Platforms Protein Structure, Tertiary Proteins Recombinant Fusion Proteins - isolation & purification Scholarships & fellowships Software Species Specificity Strategy Vaccines Vero Cells Viral antibodies Viral Envelope Proteins Viral Proteins - chemistry Viral Proteins - isolation & purification Virion - metabolism |
| title | Dengue-specific subviral nanoparticles: design, creation and characterization |
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