The mitochondrial genome encodes abundant small noncoding RNAs
Small noncoding RNAs identified thus far are all encoded by the nuclear genome. Here, we report that the murine and human mitochondrial genomes encode thousands of small noncoding RNAs, which are predominantly derived from the sense transcripts of the mitochondrial genes （host genes）, and we termed...
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| Veröffentlicht in: | Cell research 2013-06, Vol.23 (6), p.759-774 |
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| container_title | Cell research |
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| creator | Ro, Seungil Ma, Hsiu-Yen Park, Chanjae Ortogero, Nicole Song, Rui Hennig, Grant W Zheng, Huili Lin, Yung-Ming Moro, Loredana Hsieh, Jer-Tsong Yan, Wei |
| description | Small noncoding RNAs identified thus far are all encoded by the nuclear genome. Here, we report that the murine and human mitochondrial genomes encode thousands of small noncoding RNAs, which are predominantly derived from the sense transcripts of the mitochondrial genes （host genes）, and we termed these small RNAs mitochondrial genome-encoded small RNAs （mitosRNAs）. DICER inactivation affected, but did not completely abolish mitosRNA production. MitosRNAs appear to be products of currently unidentified mitochondrial ribonucleases. Overexpression of mitosRNAs enhanced expression levels of their host genes in vitro, and dysregulated mitosRNA expression was generally associated with aberrant mitochondrial gene expression in vivo. Our data demonstrate that in addition to 37 known mitochondrial genes, the mammalian mitochondrial genome also encodes abundant mitosRNAs, which may play an important regulatory role in the control of mitochondrial gene expression in the cell. |
| doi_str_mv | 10.1038/cr.2013.37 |
| format | Article |
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Here, we report that the murine and human mitochondrial genomes encode thousands of small noncoding RNAs, which are predominantly derived from the sense transcripts of the mitochondrial genes （host genes）, and we termed these small RNAs mitochondrial genome-encoded small RNAs （mitosRNAs）. DICER inactivation affected, but did not completely abolish mitosRNA production. MitosRNAs appear to be products of currently unidentified mitochondrial ribonucleases. Overexpression of mitosRNAs enhanced expression levels of their host genes in vitro, and dysregulated mitosRNA expression was generally associated with aberrant mitochondrial gene expression in vivo. Our data demonstrate that in addition to 37 known mitochondrial genes, the mammalian mitochondrial genome also encodes abundant mitosRNAs, which may play an important regulatory role in the control of mitochondrial gene expression in the cell.</description><identifier>ISSN: 1001-0602</identifier><identifier>EISSN: 1748-7838</identifier><identifier>DOI: 10.1038/cr.2013.37</identifier><identifier>PMID: 23478297</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/208/199 ; 631/208/726/2129 ; 631/337/384/521 ; Animals ; Biomedical and Life Sciences ; Cell Biology ; Cell Nucleus - genetics ; Chromosome Mapping ; DEAD-box RNA Helicases ; DICER ; Gene expression ; Genes ; Genome, Mitochondrial - genetics ; Genomes ; Humans ; Inactivation ; Life Sciences ; Mammals ; Mice ; Mitochondria ; Mitochondria - genetics ; Original ; original-article ; Ribonuclease III - metabolism ; RNA, Small Untranslated - genetics ; 基因编码 ; 基因转录 ; 小分子RNA ; 核基因组 ; 核糖核酸 ; 线粒体基因组 ; 非编码RNA</subject><ispartof>Cell research, 2013-06, Vol.23 (6), p.759-774</ispartof><rights>Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2013</rights><rights>Copyright Nature Publishing Group Jun 2013</rights><rights>Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2013.</rights><rights>Copyright © 2013 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2013 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c595t-27a01e7a2bae9b662be674ebb04d2be96576f0ef66d3d97561d9de6501dfce093</citedby><cites>FETCH-LOGICAL-c595t-27a01e7a2bae9b662be674ebb04d2be96576f0ef66d3d97561d9de6501dfce093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85240X/85240X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3674384/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3674384/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23478297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ro, Seungil</creatorcontrib><creatorcontrib>Ma, Hsiu-Yen</creatorcontrib><creatorcontrib>Park, Chanjae</creatorcontrib><creatorcontrib>Ortogero, Nicole</creatorcontrib><creatorcontrib>Song, Rui</creatorcontrib><creatorcontrib>Hennig, Grant W</creatorcontrib><creatorcontrib>Zheng, Huili</creatorcontrib><creatorcontrib>Lin, Yung-Ming</creatorcontrib><creatorcontrib>Moro, Loredana</creatorcontrib><creatorcontrib>Hsieh, Jer-Tsong</creatorcontrib><creatorcontrib>Yan, Wei</creatorcontrib><title>The mitochondrial genome encodes abundant small noncoding RNAs</title><title>Cell research</title><addtitle>Cell Res</addtitle><addtitle>Cell Research</addtitle><description>Small noncoding RNAs identified thus far are all encoded by the nuclear genome. Here, we report that the murine and human mitochondrial genomes encode thousands of small noncoding RNAs, which are predominantly derived from the sense transcripts of the mitochondrial genes （host genes）, and we termed these small RNAs mitochondrial genome-encoded small RNAs （mitosRNAs）. DICER inactivation affected, but did not completely abolish mitosRNA production. MitosRNAs appear to be products of currently unidentified mitochondrial ribonucleases. Overexpression of mitosRNAs enhanced expression levels of their host genes in vitro, and dysregulated mitosRNA expression was generally associated with aberrant mitochondrial gene expression in vivo. Our data demonstrate that in addition to 37 known mitochondrial genes, the mammalian mitochondrial genome also encodes abundant mitosRNAs, which may play an important regulatory role in the control of mitochondrial gene expression in the cell.</description><subject>631/208/199</subject><subject>631/208/726/2129</subject><subject>631/337/384/521</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Cell Nucleus - genetics</subject><subject>Chromosome Mapping</subject><subject>DEAD-box RNA Helicases</subject><subject>DICER</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genome, Mitochondrial - genetics</subject><subject>Genomes</subject><subject>Humans</subject><subject>Inactivation</subject><subject>Life Sciences</subject><subject>Mammals</subject><subject>Mice</subject><subject>Mitochondria</subject><subject>Mitochondria - genetics</subject><subject>Original</subject><subject>original-article</subject><subject>Ribonuclease III - metabolism</subject><subject>RNA, Small Untranslated - genetics</subject><subject>基因编码</subject><subject>基因转录</subject><subject>小分子RNA</subject><subject>核基因组</subject><subject>核糖核酸</subject><subject>线粒体基因组</subject><subject>非编码RNA</subject><issn>1001-0602</issn><issn>1748-7838</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkt9r1TAUx4Mobk5f_AOksheZ9Hryo0nzMhjDqTAUZD6HtDnt7WiTLWkF_3tT7vUyRcSnHHI--X75nhNCXlLYUOD1uzZuGFC-4eoROaZK1KWqef041wC0BAnsiDxL6RaAVaKiT8kR40LVTKtjcn6zxWIa5tBug3dxsGPRow8TFujb4DAVtlm8s34u0mTHsfBhvR98X3z9fJGekyedHRO-2J8n5NvV-5vLj-X1lw-fLi-uy7bS1VwyZYGisqyxqBspWYNSCWwaEC7XWlZKdoCdlI47rSpJnXYoK6CuaxE0PyHnO927pZnQtejnaEdzF4fJxh8m2MH83vHD1vThu-HZh9ciC7zZC8Rwv2CazTSkFsfRegxLMpRrrSkFAf-BSqEgj3hFT_9Ab8MSfZ6EYTkSpQyE_hdFeZXTCs1W6mxHtTGkFLE7pKNg1j2bNpp1z4arDL96OI8D-muxGXi7A1Ju-R7jA8-_yb3ee-df0N_nBwdFIamQSnL-E38Quzo</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Ro, Seungil</creator><creator>Ma, Hsiu-Yen</creator><creator>Park, Chanjae</creator><creator>Ortogero, Nicole</creator><creator>Song, Rui</creator><creator>Hennig, Grant W</creator><creator>Zheng, Huili</creator><creator>Lin, Yung-Ming</creator><creator>Moro, Loredana</creator><creator>Hsieh, Jer-Tsong</creator><creator>Yan, Wei</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130601</creationdate><title>The mitochondrial genome encodes abundant small noncoding RNAs</title><author>Ro, Seungil ; Ma, Hsiu-Yen ; Park, Chanjae ; Ortogero, Nicole ; Song, Rui ; Hennig, Grant W ; Zheng, Huili ; Lin, Yung-Ming ; Moro, Loredana ; Hsieh, Jer-Tsong ; Yan, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c595t-27a01e7a2bae9b662be674ebb04d2be96576f0ef66d3d97561d9de6501dfce093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>631/208/199</topic><topic>631/208/726/2129</topic><topic>631/337/384/521</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Cell Nucleus - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ro, Seungil</au><au>Ma, Hsiu-Yen</au><au>Park, Chanjae</au><au>Ortogero, Nicole</au><au>Song, Rui</au><au>Hennig, Grant W</au><au>Zheng, Huili</au><au>Lin, Yung-Ming</au><au>Moro, Loredana</au><au>Hsieh, Jer-Tsong</au><au>Yan, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The mitochondrial genome encodes abundant small noncoding RNAs</atitle><jtitle>Cell research</jtitle><stitle>Cell Res</stitle><addtitle>Cell Research</addtitle><date>2013-06-01</date><risdate>2013</risdate><volume>23</volume><issue>6</issue><spage>759</spage><epage>774</epage><pages>759-774</pages><issn>1001-0602</issn><eissn>1748-7838</eissn><abstract>Small noncoding RNAs identified thus far are all encoded by the nuclear genome. Here, we report that the murine and human mitochondrial genomes encode thousands of small noncoding RNAs, which are predominantly derived from the sense transcripts of the mitochondrial genes （host genes）, and we termed these small RNAs mitochondrial genome-encoded small RNAs （mitosRNAs）. DICER inactivation affected, but did not completely abolish mitosRNA production. MitosRNAs appear to be products of currently unidentified mitochondrial ribonucleases. Overexpression of mitosRNAs enhanced expression levels of their host genes in vitro, and dysregulated mitosRNA expression was generally associated with aberrant mitochondrial gene expression in vivo. Our data demonstrate that in addition to 37 known mitochondrial genes, the mammalian mitochondrial genome also encodes abundant mitosRNAs, which may play an important regulatory role in the control of mitochondrial gene expression in the cell.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>23478297</pmid><doi>10.1038/cr.2013.37</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | 631/208/199 631/208/726/2129 631/337/384/521 Animals Biomedical and Life Sciences Cell Biology Cell Nucleus - genetics Chromosome Mapping DEAD-box RNA Helicases DICER Gene expression Genes Genome, Mitochondrial - genetics Genomes Humans Inactivation Life Sciences Mammals Mice Mitochondria Mitochondria - genetics Original original-article Ribonuclease III - metabolism RNA, Small Untranslated - genetics 基因编码 基因转录 小分子RNA 核基因组 核糖核酸 线粒体基因组 非编码RNA |
| title | The mitochondrial genome encodes abundant small noncoding RNAs |
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