Malolactic enzyme from Oenococcus oeni: Heterologous expression in Escherichia coli and biochemical characterization

Malolactic enzymes (MLE) are known to directly convert L-malic acid into L-lactic acid with a catalytical requirement of nicotinamide adenine dinucleotide (NAD + ) and Mn 2+ ; however, the reaction mechanism is still unclear. To study a MLE, the structural gene from Oenococcus oeni strain DSM 20255...

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Veröffentlicht in:	Bioengineered 2013-05, Vol.4 (3), p.147-152
Hauptverfasser:	Schümann, Christina, Michlmayr, Herbert, del Hierro, Andrés M., Kulbe, Klaus D., Jiranek, Vladimir, Eder, Reinhard, Nguyen, Thu-Ha
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Enzyme Stability Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Gene Expression heterologous expression Kinetics Lactic Acid - metabolism Malate Dehydrogenase - chemistry Malate Dehydrogenase - genetics Malate Dehydrogenase - isolation & purification Malate Dehydrogenase - metabolism Malates - metabolism malolactic enzyme Molecular Weight Oenococcus - chemistry Oenococcus - enzymology Oenococcus - genetics Oenococcus oeni Research Paper
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