Basal Expression of Pluripotency-Associated Genes Can Contribute to Stemness Property and Differentiation Potential
Pluripotency and stemness is believed to be associated with high Oct-3/4 , Nanog , and Sox-2 (ONS) expression. Similar to embryonic stem cells (ESCs), high ONS expression eventually became the measure of pluripotency in any cell. The threshold expression of ONS genes that underscores pluripotency, s...
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| Veröffentlicht in: | Stem cells and development 2013-06, Vol.22 (12), p.182-1817 |
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| creator | Dadheech, Nidheesh Srivastava, Abhay Belani, Muskaan Gupta, Sharad Pal, Rajarshi Bhonde, Ramesh R. Srivastava, Anand S. Gupta, Sarita |
| description | Pluripotency and stemness is believed to be associated with high
Oct-3/4
,
Nanog
, and
Sox-2
(ONS) expression. Similar to embryonic stem cells (ESCs), high ONS expression eventually became the measure of pluripotency in any cell. The threshold expression of ONS genes that underscores pluripotency, stemness, and differentiation potential is still unclear. Therefore, we raised a question as to whether pluripotency and stemness is a function of basal ONS gene expression. To prove this, we carried out a comparative study between basal ONS expressing NIH3T3 cells with pluripotent mouse bone marrow mesenchymal stem cells (mBMSC) and mouse ESC. Our studies on cellular, molecular, and immunological biomarkers between NIH3T3 and mBMSC demonstrated stemness property of undifferentiated NIH3T3 cells that was similar to mBMSC and somewhat close to ESC as well. In vivo teratoma formation with all three germ layer derivatives strengthen the fact that these cells in spite of basal ONS gene expression can differentiate into cells of multiple lineages without any genetic modification. Conclusively, our novel findings suggested that the phenomenon of pluripotency which imparts ability for multilineage cell differentiation is not necessarily a function of high ONS gene expression. |
| doi_str_mv | 10.1089/scd.2012.0261 |
| format | Article |
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Oct-3/4
,
Nanog
, and
Sox-2
(ONS) expression. Similar to embryonic stem cells (ESCs), high ONS expression eventually became the measure of pluripotency in any cell. The threshold expression of ONS genes that underscores pluripotency, stemness, and differentiation potential is still unclear. Therefore, we raised a question as to whether pluripotency and stemness is a function of basal ONS gene expression. To prove this, we carried out a comparative study between basal ONS expressing NIH3T3 cells with pluripotent mouse bone marrow mesenchymal stem cells (mBMSC) and mouse ESC. Our studies on cellular, molecular, and immunological biomarkers between NIH3T3 and mBMSC demonstrated stemness property of undifferentiated NIH3T3 cells that was similar to mBMSC and somewhat close to ESC as well. In vivo teratoma formation with all three germ layer derivatives strengthen the fact that these cells in spite of basal ONS gene expression can differentiate into cells of multiple lineages without any genetic modification. Conclusively, our novel findings suggested that the phenomenon of pluripotency which imparts ability for multilineage cell differentiation is not necessarily a function of high ONS gene expression.</description><identifier>ISSN: 1547-3287</identifier><identifier>EISSN: 1557-8534</identifier><identifier>DOI: 10.1089/scd.2012.0261</identifier><identifier>PMID: 23343006</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Biomarkers - metabolism ; Bone Marrow Cells - cytology ; Bone Marrow Cells - metabolism ; Cell Differentiation ; Cell Survival ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - metabolism ; Gene Expression ; Germ Layers - cytology ; Germ Layers - metabolism ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Mice ; Mice, Inbred BALB C ; Nanog Homeobox Protein ; NIH 3T3 Cells ; Octamer Transcription Factor-3 - genetics ; Octamer Transcription Factor-3 - metabolism ; Original Research Reports ; Pluripotent Stem Cells - cytology ; Pluripotent Stem Cells - metabolism ; Primary Cell Culture ; SOXB1 Transcription Factors - genetics ; SOXB1 Transcription Factors - metabolism ; Teratoma - metabolism ; Teratoma - pathology</subject><ispartof>Stem cells and development, 2013-06, Vol.22 (12), p.182-1817</ispartof><rights>2013, Mary Ann Liebert, Inc.</rights><rights>Copyright 2013, Mary Ann Liebert, Inc. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-3271a5112e926462bba779e5f1d214f16535c404de2aa7ced1e73cd1b02a734f3</citedby><cites>FETCH-LOGICAL-c431t-3271a5112e926462bba779e5f1d214f16535c404de2aa7ced1e73cd1b02a734f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23343006$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dadheech, Nidheesh</creatorcontrib><creatorcontrib>Srivastava, Abhay</creatorcontrib><creatorcontrib>Belani, Muskaan</creatorcontrib><creatorcontrib>Gupta, Sharad</creatorcontrib><creatorcontrib>Pal, Rajarshi</creatorcontrib><creatorcontrib>Bhonde, Ramesh R.</creatorcontrib><creatorcontrib>Srivastava, Anand S.</creatorcontrib><creatorcontrib>Gupta, Sarita</creatorcontrib><title>Basal Expression of Pluripotency-Associated Genes Can Contribute to Stemness Property and Differentiation Potential</title><title>Stem cells and development</title><addtitle>Stem Cells Dev</addtitle><description>Pluripotency and stemness is believed to be associated with high
Oct-3/4
,
Nanog
, and
Sox-2
(ONS) expression. Similar to embryonic stem cells (ESCs), high ONS expression eventually became the measure of pluripotency in any cell. The threshold expression of ONS genes that underscores pluripotency, stemness, and differentiation potential is still unclear. Therefore, we raised a question as to whether pluripotency and stemness is a function of basal ONS gene expression. To prove this, we carried out a comparative study between basal ONS expressing NIH3T3 cells with pluripotent mouse bone marrow mesenchymal stem cells (mBMSC) and mouse ESC. Our studies on cellular, molecular, and immunological biomarkers between NIH3T3 and mBMSC demonstrated stemness property of undifferentiated NIH3T3 cells that was similar to mBMSC and somewhat close to ESC as well. In vivo teratoma formation with all three germ layer derivatives strengthen the fact that these cells in spite of basal ONS gene expression can differentiate into cells of multiple lineages without any genetic modification. Conclusively, our novel findings suggested that the phenomenon of pluripotency which imparts ability for multilineage cell differentiation is not necessarily a function of high ONS gene expression.</description><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Cell Differentiation</subject><subject>Cell Survival</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Gene Expression</subject><subject>Germ Layers - cytology</subject><subject>Germ Layers - metabolism</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nanog Homeobox Protein</subject><subject>NIH 3T3 Cells</subject><subject>Octamer Transcription Factor-3 - genetics</subject><subject>Octamer Transcription Factor-3 - metabolism</subject><subject>Original Research Reports</subject><subject>Pluripotent Stem Cells - cytology</subject><subject>Pluripotent Stem Cells - metabolism</subject><subject>Primary Cell Culture</subject><subject>SOXB1 Transcription Factors - genetics</subject><subject>SOXB1 Transcription Factors - metabolism</subject><subject>Teratoma - metabolism</subject><subject>Teratoma - pathology</subject><issn>1547-3287</issn><issn>1557-8534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAUhoMo3pduJS_QMbc2nY2g43gBwQF1HdL0VCOdpCQZcd7elFHRlask5_znO-FD6ISSCSX19CyadsIIZRPCKrqF9mlZyqIuudge70IWnNVyDx3E-EZyhNViF-0xzgUnpNpH8VJH3eP5xxAgRusd9h1e9KtgB5_AmXVxEaM3Vido8Q04iHimHZ55l4JtVglw8vgxwTJ3Il4EP0BIa6xdi69s10EAl_LwCF6MwPzoj9BOp_sIx1_nIXq-nj_Nbov7h5u72cV9YQSnKX9cUl1SymDKKlGxptFSTqHsaMuo6GhV8tIIIlpgWksDLQXJTUsbwrTkouOH6HzDHVbNElqTtwfdqyHYpQ5r5bVVfzvOvqoX_654VdUlYRlQbAAm-BgDdD-zlKjRvsr21WhfjfZz_vT3wp_0t-4c4JvAWNbO9Raa7Osf7Cde5pUB</recordid><startdate>20130615</startdate><enddate>20130615</enddate><creator>Dadheech, Nidheesh</creator><creator>Srivastava, Abhay</creator><creator>Belani, Muskaan</creator><creator>Gupta, Sharad</creator><creator>Pal, Rajarshi</creator><creator>Bhonde, Ramesh R.</creator><creator>Srivastava, Anand S.</creator><creator>Gupta, Sarita</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20130615</creationdate><title>Basal Expression of Pluripotency-Associated Genes Can Contribute to Stemness Property and Differentiation Potential</title><author>Dadheech, Nidheesh ; Srivastava, Abhay ; Belani, Muskaan ; Gupta, Sharad ; Pal, Rajarshi ; Bhonde, Ramesh R. ; Srivastava, Anand S. ; Gupta, Sarita</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-3271a5112e926462bba779e5f1d214f16535c404de2aa7ced1e73cd1b02a734f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biomarkers - metabolism</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Cell Differentiation</topic><topic>Cell Survival</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - metabolism</topic><topic>Gene Expression</topic><topic>Germ Layers - cytology</topic><topic>Germ Layers - metabolism</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nanog Homeobox Protein</topic><topic>NIH 3T3 Cells</topic><topic>Octamer Transcription Factor-3 - genetics</topic><topic>Octamer Transcription Factor-3 - metabolism</topic><topic>Original Research Reports</topic><topic>Pluripotent Stem Cells - cytology</topic><topic>Pluripotent Stem Cells - metabolism</topic><topic>Primary Cell Culture</topic><topic>SOXB1 Transcription Factors - genetics</topic><topic>SOXB1 Transcription Factors - metabolism</topic><topic>Teratoma - metabolism</topic><topic>Teratoma - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dadheech, Nidheesh</creatorcontrib><creatorcontrib>Srivastava, Abhay</creatorcontrib><creatorcontrib>Belani, Muskaan</creatorcontrib><creatorcontrib>Gupta, Sharad</creatorcontrib><creatorcontrib>Pal, Rajarshi</creatorcontrib><creatorcontrib>Bhonde, Ramesh R.</creatorcontrib><creatorcontrib>Srivastava, Anand S.</creatorcontrib><creatorcontrib>Gupta, Sarita</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Stem cells and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dadheech, Nidheesh</au><au>Srivastava, Abhay</au><au>Belani, Muskaan</au><au>Gupta, Sharad</au><au>Pal, Rajarshi</au><au>Bhonde, Ramesh R.</au><au>Srivastava, Anand S.</au><au>Gupta, Sarita</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Basal Expression of Pluripotency-Associated Genes Can Contribute to Stemness Property and Differentiation Potential</atitle><jtitle>Stem cells and development</jtitle><addtitle>Stem Cells Dev</addtitle><date>2013-06-15</date><risdate>2013</risdate><volume>22</volume><issue>12</issue><spage>182</spage><epage>1817</epage><pages>182-1817</pages><issn>1547-3287</issn><eissn>1557-8534</eissn><abstract>Pluripotency and stemness is believed to be associated with high
Oct-3/4
,
Nanog
, and
Sox-2
(ONS) expression. Similar to embryonic stem cells (ESCs), high ONS expression eventually became the measure of pluripotency in any cell. The threshold expression of ONS genes that underscores pluripotency, stemness, and differentiation potential is still unclear. Therefore, we raised a question as to whether pluripotency and stemness is a function of basal ONS gene expression. To prove this, we carried out a comparative study between basal ONS expressing NIH3T3 cells with pluripotent mouse bone marrow mesenchymal stem cells (mBMSC) and mouse ESC. Our studies on cellular, molecular, and immunological biomarkers between NIH3T3 and mBMSC demonstrated stemness property of undifferentiated NIH3T3 cells that was similar to mBMSC and somewhat close to ESC as well. In vivo teratoma formation with all three germ layer derivatives strengthen the fact that these cells in spite of basal ONS gene expression can differentiate into cells of multiple lineages without any genetic modification. Conclusively, our novel findings suggested that the phenomenon of pluripotency which imparts ability for multilineage cell differentiation is not necessarily a function of high ONS gene expression.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>23343006</pmid><doi>10.1089/scd.2012.0261</doi><tpages>1636</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Stem cells and development, 2013-06, Vol.22 (12), p.182-1817 |
| issn | 1547-3287 1557-8534 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3668502 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biomarkers - metabolism Bone Marrow Cells - cytology Bone Marrow Cells - metabolism Cell Differentiation Cell Survival Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Gene Expression Germ Layers - cytology Germ Layers - metabolism Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Mice Mice, Inbred BALB C Nanog Homeobox Protein NIH 3T3 Cells Octamer Transcription Factor-3 - genetics Octamer Transcription Factor-3 - metabolism Original Research Reports Pluripotent Stem Cells - cytology Pluripotent Stem Cells - metabolism Primary Cell Culture SOXB1 Transcription Factors - genetics SOXB1 Transcription Factors - metabolism Teratoma - metabolism Teratoma - pathology |
| title | Basal Expression of Pluripotency-Associated Genes Can Contribute to Stemness Property and Differentiation Potential |
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