Tyrosinase-related protein 1 mRNA expression in lymph node metastases predicts overall survival in high-risk melanoma patients
Background: Clinical outcome of high-risk melanoma patients is not reliably predicted from histopathological analyses of primary tumours and is often adjusted during disease progression. Our study aimed at extending our previous findings in skin metastases to evaluate the prognostic value of tyrosin...
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| Veröffentlicht in: | British journal of cancer 2013-04, Vol.108 (8), p.1641-1647 |
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| creator | El Hajj, P Journe, F Wiedig, M Laios, I Salès, F Galibert, M-D Van Kempen, L C Spatz, A Badran, B Larsimont, D Awada, A Ghanem, G |
| description | Background:
Clinical outcome of high-risk melanoma patients is not reliably predicted from histopathological analyses of primary tumours and is often adjusted during disease progression. Our study aimed at extending our previous findings in skin metastases to evaluate the prognostic value of tyrosinase-related protein 1 (TYRP1) in lymph node metastases of stages III and IV melanoma patients.
Methods:
TYRP1 mRNA expression in 104 lymph node metastases was quantified by real-time PCR and normalised to S100 calcium-binding protein B (S100B) mRNA expression to correct for tumour load. TYRP1/S100B ratios were calculated and median was used as cutoff value. TYRP1/S100B mRNA values were correlated to clinical follow-up and histopathological characteristics of the primary lesion.
Results:
A high TYRP1/S100B mRNA ratio significantly correlated with reduced disease-free (DFS) and overall survival (OS; Cox regression analysis,
P
=0.005 and 0.01, respectively), increased Breslow thickness (Spearman’s rho test,
P |
| doi_str_mv | 10.1038/bjc.2013.115 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3668475</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1367485565</sourcerecordid><originalsourceid>FETCH-LOGICAL-c547t-b8a928fba0cd62b0f53b2a6de86230c21366fcec262fd2fc46eb642166d3d3f23</originalsourceid><addsrcrecordid>eNqNkt-L1DAQx4so3nr65rMERFCwa340afdFWA71hEVBzueQptNt1rbpJe3ivvi3O3XX8zx8EAIhmc_MdzL5JslTRpeMiuJNubNLTplYMibvJQsmBU9ZwfP7yYJSmqd0xelZ8ijGHR5XtMgfJmdcSLaiUi6SH1eH4KPrTYQ0QGtGqMgQ_AiuJ4x0Xz6tCXwfAsTofE_wsj10Q0N6XwHpYDQRF0RMgcrZMRK_h2DalsQp7N3etHNK47ZNGlz8hhmt6X1nyGBGB_0YHycPatNGeHLaz5Ov799dXVymm88fPl6sN6mVWT6mZWFWvKhLQ22leElrKUpuVAWF4oJazoRStQXLFa8rXttMQakyzpSqRCVqLs6Tt8e6w1R2UFnUxjb1EFxnwkF74_Tfkd41euv3GgsXWS6xwKtjgeZO2uV6o-c7yqjKskLsGbIvT2LBX08QR925aKHFt4OfosZu86yQUsn_QLM8L_JczujzO-jOT6HHqf2iBFpAztqvj5TFb40B6ptmGdWzXzT6Rc9-0egXxJ_dnssN_NsgCLw4ASZa09bB9NbFPxzqcnwLcumRixjqtxBudfcv4Z_JItjT</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1347301351</pqid></control><display><type>article</type><title>Tyrosinase-related protein 1 mRNA expression in lymph node metastases predicts overall survival in high-risk melanoma patients</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>Nature</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>El Hajj, P ; Journe, F ; Wiedig, M ; Laios, I ; Salès, F ; Galibert, M-D ; Van Kempen, L C ; Spatz, A ; Badran, B ; Larsimont, D ; Awada, A ; Ghanem, G</creator><creatorcontrib>El Hajj, P ; Journe, F ; Wiedig, M ; Laios, I ; Salès, F ; Galibert, M-D ; Van Kempen, L C ; Spatz, A ; Badran, B ; Larsimont, D ; Awada, A ; Ghanem, G</creatorcontrib><description>Background:
Clinical outcome of high-risk melanoma patients is not reliably predicted from histopathological analyses of primary tumours and is often adjusted during disease progression. Our study aimed at extending our previous findings in skin metastases to evaluate the prognostic value of tyrosinase-related protein 1 (TYRP1) in lymph node metastases of stages III and IV melanoma patients.
Methods:
TYRP1 mRNA expression in 104 lymph node metastases was quantified by real-time PCR and normalised to S100 calcium-binding protein B (S100B) mRNA expression to correct for tumour load. TYRP1/S100B ratios were calculated and median was used as cutoff value. TYRP1/S100B mRNA values were correlated to clinical follow-up and histopathological characteristics of the primary lesion.
Results:
A high TYRP1/S100B mRNA ratio significantly correlated with reduced disease-free (DFS) and overall survival (OS; Cox regression analysis,
P
=0.005 and 0.01, respectively), increased Breslow thickness (Spearman’s rho test,
P
<0.001) and the presence of ulceration (Mann–Whitney test,
P
=0.02) of the primaries. Moreover, high TYRP1/S100B was of better prognostic value (lower
P
-value) for OS than Breslow thickness and ulceration. Finally, it was well conserved during disease progression with respect to high/low TYRP1 groups.
Conclusion:
High TYRP1/S100B mRNA expression in lymph node metastases from melanoma patients is associated with unfavourable clinical outcome. Its evaluation in lymph node metastases may refine initial prognosis for metastatic patients, may define prognosis for those with unknown or non-evaluable primary lesions and may allow different management of the two groups of patients.</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1038/bjc.2013.115</identifier><identifier>PMID: 23519055</identifier><identifier>CODEN: BJCAAI</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/250/1620/1616 ; 631/67/322 ; 692/53/2422 ; 692/699/67/1813/1634 ; Adolescent ; Adult ; Aged ; Biological and medical sciences ; Biomarkers, Tumor - biosynthesis ; Biomarkers, Tumor - genetics ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; Disease-Free Survival ; Drug Resistance ; Epidemiology ; Female ; Genetics ; Hematologic and hematopoietic diseases ; Humans ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Life Sciences ; Lymph Nodes - metabolism ; Lymph Nodes - pathology ; Lymphatic Metastasis ; Lymphatic system ; Male ; Medical prognosis ; Medical research ; Medical sciences ; Melanoma ; Melanoma - enzymology ; Melanoma - genetics ; Melanoma - pathology ; Membrane Glycoproteins - biosynthesis ; Membrane Glycoproteins - genetics ; Metastasis ; Middle Aged ; Molecular Diagnostics ; Molecular Medicine ; Multiple tumors. Solid tumors. Tumors in childhood (general aspects) ; Nerve Growth Factors - biosynthesis ; Nerve Growth Factors - genetics ; Oncology ; Oxidoreductases - biosynthesis ; Oxidoreductases - genetics ; Pathology ; Proteins ; Real-Time Polymerase Chain Reaction ; Risk Factors ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; S100 Calcium Binding Protein beta Subunit ; S100 Proteins - biosynthesis ; S100 Proteins - genetics ; Skin cancer ; Survival Rate ; Tumors ; Young Adult</subject><ispartof>British journal of cancer, 2013-04, Vol.108 (8), p.1641-1647</ispartof><rights>The Author(s) 2013</rights><rights>2014 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Apr 30, 2013</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Copyright © 2013 Cancer Research UK 2013 Cancer Research UK</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c547t-b8a928fba0cd62b0f53b2a6de86230c21366fcec262fd2fc46eb642166d3d3f23</citedby><cites>FETCH-LOGICAL-c547t-b8a928fba0cd62b0f53b2a6de86230c21366fcec262fd2fc46eb642166d3d3f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668475/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668475/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27302485$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23519055$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01064483$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>El Hajj, P</creatorcontrib><creatorcontrib>Journe, F</creatorcontrib><creatorcontrib>Wiedig, M</creatorcontrib><creatorcontrib>Laios, I</creatorcontrib><creatorcontrib>Salès, F</creatorcontrib><creatorcontrib>Galibert, M-D</creatorcontrib><creatorcontrib>Van Kempen, L C</creatorcontrib><creatorcontrib>Spatz, A</creatorcontrib><creatorcontrib>Badran, B</creatorcontrib><creatorcontrib>Larsimont, D</creatorcontrib><creatorcontrib>Awada, A</creatorcontrib><creatorcontrib>Ghanem, G</creatorcontrib><title>Tyrosinase-related protein 1 mRNA expression in lymph node metastases predicts overall survival in high-risk melanoma patients</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><addtitle>Br J Cancer</addtitle><description>Background:
Clinical outcome of high-risk melanoma patients is not reliably predicted from histopathological analyses of primary tumours and is often adjusted during disease progression. Our study aimed at extending our previous findings in skin metastases to evaluate the prognostic value of tyrosinase-related protein 1 (TYRP1) in lymph node metastases of stages III and IV melanoma patients.
Methods:
TYRP1 mRNA expression in 104 lymph node metastases was quantified by real-time PCR and normalised to S100 calcium-binding protein B (S100B) mRNA expression to correct for tumour load. TYRP1/S100B ratios were calculated and median was used as cutoff value. TYRP1/S100B mRNA values were correlated to clinical follow-up and histopathological characteristics of the primary lesion.
Results:
A high TYRP1/S100B mRNA ratio significantly correlated with reduced disease-free (DFS) and overall survival (OS; Cox regression analysis,
P
=0.005 and 0.01, respectively), increased Breslow thickness (Spearman’s rho test,
P
<0.001) and the presence of ulceration (Mann–Whitney test,
P
=0.02) of the primaries. Moreover, high TYRP1/S100B was of better prognostic value (lower
P
-value) for OS than Breslow thickness and ulceration. Finally, it was well conserved during disease progression with respect to high/low TYRP1 groups.
Conclusion:
High TYRP1/S100B mRNA expression in lymph node metastases from melanoma patients is associated with unfavourable clinical outcome. Its evaluation in lymph node metastases may refine initial prognosis for metastatic patients, may define prognosis for those with unknown or non-evaluable primary lesions and may allow different management of the two groups of patients.</description><subject>631/250/1620/1616</subject><subject>631/67/322</subject><subject>692/53/2422</subject><subject>692/699/67/1813/1634</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - biosynthesis</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Disease-Free Survival</subject><subject>Drug Resistance</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Genetics</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Life Sciences</subject><subject>Lymph Nodes - metabolism</subject><subject>Lymph Nodes - pathology</subject><subject>Lymphatic Metastasis</subject><subject>Lymphatic system</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Melanoma</subject><subject>Melanoma - enzymology</subject><subject>Melanoma - genetics</subject><subject>Melanoma - pathology</subject><subject>Membrane Glycoproteins - biosynthesis</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Metastasis</subject><subject>Middle Aged</subject><subject>Molecular Diagnostics</subject><subject>Molecular Medicine</subject><subject>Multiple tumors. Solid tumors. Tumors in childhood (general aspects)</subject><subject>Nerve Growth Factors - biosynthesis</subject><subject>Nerve Growth Factors - genetics</subject><subject>Oncology</subject><subject>Oxidoreductases - biosynthesis</subject><subject>Oxidoreductases - genetics</subject><subject>Pathology</subject><subject>Proteins</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Risk Factors</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>S100 Calcium Binding Protein beta Subunit</subject><subject>S100 Proteins - biosynthesis</subject><subject>S100 Proteins - genetics</subject><subject>Skin cancer</subject><subject>Survival Rate</subject><subject>Tumors</subject><subject>Young Adult</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkt-L1DAQx4so3nr65rMERFCwa340afdFWA71hEVBzueQptNt1rbpJe3ivvi3O3XX8zx8EAIhmc_MdzL5JslTRpeMiuJNubNLTplYMibvJQsmBU9ZwfP7yYJSmqd0xelZ8ijGHR5XtMgfJmdcSLaiUi6SH1eH4KPrTYQ0QGtGqMgQ_AiuJ4x0Xz6tCXwfAsTofE_wsj10Q0N6XwHpYDQRF0RMgcrZMRK_h2DalsQp7N3etHNK47ZNGlz8hhmt6X1nyGBGB_0YHycPatNGeHLaz5Ov799dXVymm88fPl6sN6mVWT6mZWFWvKhLQ22leElrKUpuVAWF4oJazoRStQXLFa8rXttMQakyzpSqRCVqLs6Tt8e6w1R2UFnUxjb1EFxnwkF74_Tfkd41euv3GgsXWS6xwKtjgeZO2uV6o-c7yqjKskLsGbIvT2LBX08QR925aKHFt4OfosZu86yQUsn_QLM8L_JczujzO-jOT6HHqf2iBFpAztqvj5TFb40B6ptmGdWzXzT6Rc9-0egXxJ_dnssN_NsgCLw4ASZa09bB9NbFPxzqcnwLcumRixjqtxBudfcv4Z_JItjT</recordid><startdate>20130430</startdate><enddate>20130430</enddate><creator>El Hajj, P</creator><creator>Journe, F</creator><creator>Wiedig, M</creator><creator>Laios, I</creator><creator>Salès, F</creator><creator>Galibert, M-D</creator><creator>Van Kempen, L C</creator><creator>Spatz, A</creator><creator>Badran, B</creator><creator>Larsimont, D</creator><creator>Awada, A</creator><creator>Ghanem, G</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><general>Cancer Research UK</general><scope>C6C</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7TM</scope><scope>1XC</scope><scope>5PM</scope></search><sort><creationdate>20130430</creationdate><title>Tyrosinase-related protein 1 mRNA expression in lymph node metastases predicts overall survival in high-risk melanoma patients</title><author>El Hajj, P ; Journe, F ; Wiedig, M ; Laios, I ; Salès, F ; Galibert, M-D ; Van Kempen, L C ; Spatz, A ; Badran, B ; Larsimont, D ; Awada, A ; Ghanem, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c547t-b8a928fba0cd62b0f53b2a6de86230c21366fcec262fd2fc46eb642166d3d3f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>631/250/1620/1616</topic><topic>631/67/322</topic><topic>692/53/2422</topic><topic>692/699/67/1813/1634</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - biosynthesis</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>Disease-Free Survival</topic><topic>Drug Resistance</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Genetics</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Life Sciences</topic><topic>Lymph Nodes - metabolism</topic><topic>Lymph Nodes - pathology</topic><topic>Lymphatic Metastasis</topic><topic>Lymphatic system</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>Medical research</topic><topic>Medical sciences</topic><topic>Melanoma</topic><topic>Melanoma - enzymology</topic><topic>Melanoma - genetics</topic><topic>Melanoma - pathology</topic><topic>Membrane Glycoproteins - biosynthesis</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Metastasis</topic><topic>Middle Aged</topic><topic>Molecular Diagnostics</topic><topic>Molecular Medicine</topic><topic>Multiple tumors. Solid tumors. Tumors in childhood (general aspects)</topic><topic>Nerve Growth Factors - biosynthesis</topic><topic>Nerve Growth Factors - genetics</topic><topic>Oncology</topic><topic>Oxidoreductases - biosynthesis</topic><topic>Oxidoreductases - genetics</topic><topic>Pathology</topic><topic>Proteins</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Risk Factors</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>S100 Calcium Binding Protein beta Subunit</topic><topic>S100 Proteins - biosynthesis</topic><topic>S100 Proteins - genetics</topic><topic>Skin cancer</topic><topic>Survival Rate</topic><topic>Tumors</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El Hajj, P</creatorcontrib><creatorcontrib>Journe, F</creatorcontrib><creatorcontrib>Wiedig, M</creatorcontrib><creatorcontrib>Laios, I</creatorcontrib><creatorcontrib>Salès, F</creatorcontrib><creatorcontrib>Galibert, M-D</creatorcontrib><creatorcontrib>Van Kempen, L C</creatorcontrib><creatorcontrib>Spatz, A</creatorcontrib><creatorcontrib>Badran, B</creatorcontrib><creatorcontrib>Larsimont, D</creatorcontrib><creatorcontrib>Awada, A</creatorcontrib><creatorcontrib>Ghanem, G</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El Hajj, P</au><au>Journe, F</au><au>Wiedig, M</au><au>Laios, I</au><au>Salès, F</au><au>Galibert, M-D</au><au>Van Kempen, L C</au><au>Spatz, A</au><au>Badran, B</au><au>Larsimont, D</au><au>Awada, A</au><au>Ghanem, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tyrosinase-related protein 1 mRNA expression in lymph node metastases predicts overall survival in high-risk melanoma patients</atitle><jtitle>British journal of cancer</jtitle><stitle>Br J Cancer</stitle><addtitle>Br J Cancer</addtitle><date>2013-04-30</date><risdate>2013</risdate><volume>108</volume><issue>8</issue><spage>1641</spage><epage>1647</epage><pages>1641-1647</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><coden>BJCAAI</coden><abstract>Background:
Clinical outcome of high-risk melanoma patients is not reliably predicted from histopathological analyses of primary tumours and is often adjusted during disease progression. Our study aimed at extending our previous findings in skin metastases to evaluate the prognostic value of tyrosinase-related protein 1 (TYRP1) in lymph node metastases of stages III and IV melanoma patients.
Methods:
TYRP1 mRNA expression in 104 lymph node metastases was quantified by real-time PCR and normalised to S100 calcium-binding protein B (S100B) mRNA expression to correct for tumour load. TYRP1/S100B ratios were calculated and median was used as cutoff value. TYRP1/S100B mRNA values were correlated to clinical follow-up and histopathological characteristics of the primary lesion.
Results:
A high TYRP1/S100B mRNA ratio significantly correlated with reduced disease-free (DFS) and overall survival (OS; Cox regression analysis,
P
=0.005 and 0.01, respectively), increased Breslow thickness (Spearman’s rho test,
P
<0.001) and the presence of ulceration (Mann–Whitney test,
P
=0.02) of the primaries. Moreover, high TYRP1/S100B was of better prognostic value (lower
P
-value) for OS than Breslow thickness and ulceration. Finally, it was well conserved during disease progression with respect to high/low TYRP1 groups.
Conclusion:
High TYRP1/S100B mRNA expression in lymph node metastases from melanoma patients is associated with unfavourable clinical outcome. Its evaluation in lymph node metastases may refine initial prognosis for metastatic patients, may define prognosis for those with unknown or non-evaluable primary lesions and may allow different management of the two groups of patients.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>23519055</pmid><doi>10.1038/bjc.2013.115</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0007-0920 1532-1827 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3668475 |
| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | 631/250/1620/1616 631/67/322 692/53/2422 692/699/67/1813/1634 Adolescent Adult Aged Biological and medical sciences Biomarkers, Tumor - biosynthesis Biomarkers, Tumor - genetics Biomedical and Life Sciences Biomedicine Cancer Research Disease-Free Survival Drug Resistance Epidemiology Female Genetics Hematologic and hematopoietic diseases Humans Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Life Sciences Lymph Nodes - metabolism Lymph Nodes - pathology Lymphatic Metastasis Lymphatic system Male Medical prognosis Medical research Medical sciences Melanoma Melanoma - enzymology Melanoma - genetics Melanoma - pathology Membrane Glycoproteins - biosynthesis Membrane Glycoproteins - genetics Metastasis Middle Aged Molecular Diagnostics Molecular Medicine Multiple tumors. Solid tumors. Tumors in childhood (general aspects) Nerve Growth Factors - biosynthesis Nerve Growth Factors - genetics Oncology Oxidoreductases - biosynthesis Oxidoreductases - genetics Pathology Proteins Real-Time Polymerase Chain Reaction Risk Factors RNA, Messenger - biosynthesis RNA, Messenger - genetics S100 Calcium Binding Protein beta Subunit S100 Proteins - biosynthesis S100 Proteins - genetics Skin cancer Survival Rate Tumors Young Adult |
| title | Tyrosinase-related protein 1 mRNA expression in lymph node metastases predicts overall survival in high-risk melanoma patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T12%3A38%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Tyrosinase-related%20protein%201%20mRNA%20expression%20in%20lymph%20node%20metastases%20predicts%20overall%20survival%20in%20high-risk%20melanoma%20patients&rft.jtitle=British%20journal%20of%20cancer&rft.au=El%20Hajj,%20P&rft.date=2013-04-30&rft.volume=108&rft.issue=8&rft.spage=1641&rft.epage=1647&rft.pages=1641-1647&rft.issn=0007-0920&rft.eissn=1532-1827&rft.coden=BJCAAI&rft_id=info:doi/10.1038/bjc.2013.115&rft_dat=%3Cproquest_pubme%3E1367485565%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1347301351&rft_id=info:pmid/23519055&rfr_iscdi=true |