A short-term colorectal cancer sphere culture as a relevant tool for human cancer biology investigation
Background: Ex vivo colospheres have been previously characterised as a colorectal cancer (CRC) well-rounded multicellular model, exclusively formed by carcinoma cells, and derived from fresh CRC tissue after mechanical dissociation. The ability to form colospheres was correlated with tumour aggress...
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| Veröffentlicht in: | British journal of cancer 2013-04, Vol.108 (8), p.1720-1731 |
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| creator | Weiswald, L-B Richon, S Massonnet, G Guinebretière, J-M Vacher, S Laurendeau, I Cottu, P Marangoni, E Nemati, F Validire, P Bellet, D Bièche, I Dangles-Marie, V |
| description | Background:
Ex vivo
colospheres have been previously characterised as a colorectal cancer (CRC) well-rounded multicellular model, exclusively formed by carcinoma cells, and derived from fresh CRC tissue after mechanical dissociation. The ability to form colospheres was correlated with tumour aggressiveness. Their three-dimensional conformation prompted us to further investigate their potential interest as a preclinical cancer tool.
Methods:
Patient-derived CRC xenografts were used to produce numerous colospheres. Mechanism of formation was elucidated by confocal microscopy. Expression analysis of a panel of 64 selected cancer-related genes by real-time qRT–PCR and hierarchical clustering allowed comparison of colospheres with parent xenografts.
In vitro
and
in vivo
assays were performed for migration and chemosensitivity studies.
Results:
Colospheres, formed by tissue remodelling and compaction, remained viable several weeks in floating conditions, escaping anoikis through their strong cell–cell interactions. Colospheres matched the gene expression profile of the parent xenograft tissue. Colosphere-forming cells migrated in collagen I matrix and metastasised when subrenally implanted in
nude
mice. Besides, the colosphere responses to 5-fluorouracil and irinotecan, two standard drugs in CRC, reproduced those of the
in vivo
original xenografts.
Conclusion:
Colospheres closely mimic biological characteristics of
in vivo
CRC tumours. Consequently, they would be relevant
ex vivo
CRC models. |
| doi_str_mv | 10.1038/bjc.2013.132 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3668460</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2958610861</sourcerecordid><originalsourceid>FETCH-LOGICAL-c546t-bb40f6172e79c4d411556d135528634d70a80757525ad895630bda89e321a63a3</originalsourceid><addsrcrecordid>eNptkc1r3DAQxUVpabZpbz0XQemt3uhb8qUQQj8CgV7SsxjLsteLV9pK8kL--yjsJk0hp0HMb948zUPoIyVrSri56LZuzQjla8rZK7SikrOGGqZfoxUhRDekZeQMvct5W58tMfotOmNccsONXqHxEudNTKUpPu2wi3NM3hWYsYPgfMJ5v_HJY7fMZakVMgac_OwPEAouMc54iAlvlh2Ex5FuqirjHZ7CwecyjVCmGN6jNwPM2X841XP058f326tfzc3vn9dXlzeNk0KVpusEGRTVzOvWiV5QKqXqKZeSGcVFrwkYoqWWTEJvWqk46XowreeMguLAz9G3o-5-6Xa-dz6UBLPdp2kH6c5GmOz_nTBt7BgPlitlhCJV4PNJIMW_S_2A3cYlherZUi40r5emplJfj5RLMefkh6cNlNiHWGyNxT7EUqdYxT89d_UEP-ZQgS8nALKDeUj1lFP-x9W9TLSics2Ry7UVRp-euXtp8T2l56T1</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1347301318</pqid></control><display><type>article</type><title>A short-term colorectal cancer sphere culture as a relevant tool for human cancer biology investigation</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>Nature</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Weiswald, L-B ; Richon, S ; Massonnet, G ; Guinebretière, J-M ; Vacher, S ; Laurendeau, I ; Cottu, P ; Marangoni, E ; Nemati, F ; Validire, P ; Bellet, D ; Bièche, I ; Dangles-Marie, V</creator><creatorcontrib>Weiswald, L-B ; Richon, S ; Massonnet, G ; Guinebretière, J-M ; Vacher, S ; Laurendeau, I ; Cottu, P ; Marangoni, E ; Nemati, F ; Validire, P ; Bellet, D ; Bièche, I ; Dangles-Marie, V</creatorcontrib><description>Background:
Ex vivo
colospheres have been previously characterised as a colorectal cancer (CRC) well-rounded multicellular model, exclusively formed by carcinoma cells, and derived from fresh CRC tissue after mechanical dissociation. The ability to form colospheres was correlated with tumour aggressiveness. Their three-dimensional conformation prompted us to further investigate their potential interest as a preclinical cancer tool.
Methods:
Patient-derived CRC xenografts were used to produce numerous colospheres. Mechanism of formation was elucidated by confocal microscopy. Expression analysis of a panel of 64 selected cancer-related genes by real-time qRT–PCR and hierarchical clustering allowed comparison of colospheres with parent xenografts.
In vitro
and
in vivo
assays were performed for migration and chemosensitivity studies.
Results:
Colospheres, formed by tissue remodelling and compaction, remained viable several weeks in floating conditions, escaping anoikis through their strong cell–cell interactions. Colospheres matched the gene expression profile of the parent xenograft tissue. Colosphere-forming cells migrated in collagen I matrix and metastasised when subrenally implanted in
nude
mice. Besides, the colosphere responses to 5-fluorouracil and irinotecan, two standard drugs in CRC, reproduced those of the
in vivo
original xenografts.
Conclusion:
Colospheres closely mimic biological characteristics of
in vivo
CRC tumours. Consequently, they would be relevant
ex vivo
CRC models.</description><identifier>ISSN: 0007-0920</identifier><identifier>EISSN: 1532-1827</identifier><identifier>DOI: 10.1038/bjc.2013.132</identifier><identifier>PMID: 23538387</identifier><identifier>CODEN: BJCAAI</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/67/70 ; 692/308 ; 692/699/67/1504/1885 ; Animals ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Camptothecin - analogs & derivatives ; Camptothecin - pharmacology ; Cancer Research ; Cancer therapies ; Cell Line, Tumor ; Cell Movement - physiology ; Cell Survival - physiology ; Colorectal cancer ; Colorectal Neoplasms - drug therapy ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - pathology ; Drug Resistance ; Drug Screening Assays, Antitumor ; Epidemiology ; Female ; Fluorouracil - pharmacology ; Gastroenterology. Liver. Pancreas. Abdomen ; Gene expression ; Humans ; Medical sciences ; Metastasis ; Mice ; Mice, Nude ; Mice, SCID ; Microscopy, Confocal ; Molecular Diagnostics ; Molecular Medicine ; Multiple tumors. Solid tumors. Tumors in childhood (general aspects) ; Neoplasm Transplantation ; Oncology ; Random Allocation ; Real-Time Polymerase Chain Reaction ; Spheroids, Cellular - pathology ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Transplantation, Heterologous ; Tumors ; Xenograft Model Antitumor Assays</subject><ispartof>British journal of cancer, 2013-04, Vol.108 (8), p.1720-1731</ispartof><rights>The Author(s) 2013</rights><rights>2014 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Apr 30, 2013</rights><rights>Copyright © 2013 Cancer Research UK 2013 Cancer Research UK</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c546t-bb40f6172e79c4d411556d135528634d70a80757525ad895630bda89e321a63a3</citedby><cites>FETCH-LOGICAL-c546t-bb40f6172e79c4d411556d135528634d70a80757525ad895630bda89e321a63a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668460/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668460/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27302494$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23538387$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weiswald, L-B</creatorcontrib><creatorcontrib>Richon, S</creatorcontrib><creatorcontrib>Massonnet, G</creatorcontrib><creatorcontrib>Guinebretière, J-M</creatorcontrib><creatorcontrib>Vacher, S</creatorcontrib><creatorcontrib>Laurendeau, I</creatorcontrib><creatorcontrib>Cottu, P</creatorcontrib><creatorcontrib>Marangoni, E</creatorcontrib><creatorcontrib>Nemati, F</creatorcontrib><creatorcontrib>Validire, P</creatorcontrib><creatorcontrib>Bellet, D</creatorcontrib><creatorcontrib>Bièche, I</creatorcontrib><creatorcontrib>Dangles-Marie, V</creatorcontrib><title>A short-term colorectal cancer sphere culture as a relevant tool for human cancer biology investigation</title><title>British journal of cancer</title><addtitle>Br J Cancer</addtitle><addtitle>Br J Cancer</addtitle><description>Background:
Ex vivo
colospheres have been previously characterised as a colorectal cancer (CRC) well-rounded multicellular model, exclusively formed by carcinoma cells, and derived from fresh CRC tissue after mechanical dissociation. The ability to form colospheres was correlated with tumour aggressiveness. Their three-dimensional conformation prompted us to further investigate their potential interest as a preclinical cancer tool.
Methods:
Patient-derived CRC xenografts were used to produce numerous colospheres. Mechanism of formation was elucidated by confocal microscopy. Expression analysis of a panel of 64 selected cancer-related genes by real-time qRT–PCR and hierarchical clustering allowed comparison of colospheres with parent xenografts.
In vitro
and
in vivo
assays were performed for migration and chemosensitivity studies.
Results:
Colospheres, formed by tissue remodelling and compaction, remained viable several weeks in floating conditions, escaping anoikis through their strong cell–cell interactions. Colospheres matched the gene expression profile of the parent xenograft tissue. Colosphere-forming cells migrated in collagen I matrix and metastasised when subrenally implanted in
nude
mice. Besides, the colosphere responses to 5-fluorouracil and irinotecan, two standard drugs in CRC, reproduced those of the
in vivo
original xenografts.
Conclusion:
Colospheres closely mimic biological characteristics of
in vivo
CRC tumours. Consequently, they would be relevant
ex vivo
CRC models.</description><subject>631/67/70</subject><subject>692/308</subject><subject>692/699/67/1504/1885</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Camptothecin - analogs & derivatives</subject><subject>Camptothecin - pharmacology</subject><subject>Cancer Research</subject><subject>Cancer therapies</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - physiology</subject><subject>Cell Survival - physiology</subject><subject>Colorectal cancer</subject><subject>Colorectal Neoplasms - drug therapy</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Drug Resistance</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Epidemiology</subject><subject>Female</subject><subject>Fluorouracil - pharmacology</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Metastasis</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Mice, SCID</subject><subject>Microscopy, Confocal</subject><subject>Molecular Diagnostics</subject><subject>Molecular Medicine</subject><subject>Multiple tumors. Solid tumors. Tumors in childhood (general aspects)</subject><subject>Neoplasm Transplantation</subject><subject>Oncology</subject><subject>Random Allocation</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Spheroids, Cellular - pathology</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Transplantation, Heterologous</subject><subject>Tumors</subject><subject>Xenograft Model Antitumor Assays</subject><issn>0007-0920</issn><issn>1532-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkc1r3DAQxUVpabZpbz0XQemt3uhb8qUQQj8CgV7SsxjLsteLV9pK8kL--yjsJk0hp0HMb948zUPoIyVrSri56LZuzQjla8rZK7SikrOGGqZfoxUhRDekZeQMvct5W58tMfotOmNccsONXqHxEudNTKUpPu2wi3NM3hWYsYPgfMJ5v_HJY7fMZakVMgac_OwPEAouMc54iAlvlh2Ex5FuqirjHZ7CwecyjVCmGN6jNwPM2X841XP058f326tfzc3vn9dXlzeNk0KVpusEGRTVzOvWiV5QKqXqKZeSGcVFrwkYoqWWTEJvWqk46XowreeMguLAz9G3o-5-6Xa-dz6UBLPdp2kH6c5GmOz_nTBt7BgPlitlhCJV4PNJIMW_S_2A3cYlherZUi40r5emplJfj5RLMefkh6cNlNiHWGyNxT7EUqdYxT89d_UEP-ZQgS8nALKDeUj1lFP-x9W9TLSics2Ry7UVRp-euXtp8T2l56T1</recordid><startdate>20130430</startdate><enddate>20130430</enddate><creator>Weiswald, L-B</creator><creator>Richon, S</creator><creator>Massonnet, G</creator><creator>Guinebretière, J-M</creator><creator>Vacher, S</creator><creator>Laurendeau, I</creator><creator>Cottu, P</creator><creator>Marangoni, E</creator><creator>Nemati, F</creator><creator>Validire, P</creator><creator>Bellet, D</creator><creator>Bièche, I</creator><creator>Dangles-Marie, V</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20130430</creationdate><title>A short-term colorectal cancer sphere culture as a relevant tool for human cancer biology investigation</title><author>Weiswald, L-B ; Richon, S ; Massonnet, G ; Guinebretière, J-M ; Vacher, S ; Laurendeau, I ; Cottu, P ; Marangoni, E ; Nemati, F ; Validire, P ; Bellet, D ; Bièche, I ; Dangles-Marie, V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c546t-bb40f6172e79c4d411556d135528634d70a80757525ad895630bda89e321a63a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>631/67/70</topic><topic>692/308</topic><topic>692/699/67/1504/1885</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Camptothecin - analogs & derivatives</topic><topic>Camptothecin - pharmacology</topic><topic>Cancer Research</topic><topic>Cancer therapies</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - physiology</topic><topic>Cell Survival - physiology</topic><topic>Colorectal cancer</topic><topic>Colorectal Neoplasms - drug therapy</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Drug Resistance</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Epidemiology</topic><topic>Female</topic><topic>Fluorouracil - pharmacology</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Metastasis</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Mice, SCID</topic><topic>Microscopy, Confocal</topic><topic>Molecular Diagnostics</topic><topic>Molecular Medicine</topic><topic>Multiple tumors. Solid tumors. Tumors in childhood (general aspects)</topic><topic>Neoplasm Transplantation</topic><topic>Oncology</topic><topic>Random Allocation</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Spheroids, Cellular - pathology</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</topic><topic>Transplantation, Heterologous</topic><topic>Tumors</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weiswald, L-B</creatorcontrib><creatorcontrib>Richon, S</creatorcontrib><creatorcontrib>Massonnet, G</creatorcontrib><creatorcontrib>Guinebretière, J-M</creatorcontrib><creatorcontrib>Vacher, S</creatorcontrib><creatorcontrib>Laurendeau, I</creatorcontrib><creatorcontrib>Cottu, P</creatorcontrib><creatorcontrib>Marangoni, E</creatorcontrib><creatorcontrib>Nemati, F</creatorcontrib><creatorcontrib>Validire, P</creatorcontrib><creatorcontrib>Bellet, D</creatorcontrib><creatorcontrib>Bièche, I</creatorcontrib><creatorcontrib>Dangles-Marie, V</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weiswald, L-B</au><au>Richon, S</au><au>Massonnet, G</au><au>Guinebretière, J-M</au><au>Vacher, S</au><au>Laurendeau, I</au><au>Cottu, P</au><au>Marangoni, E</au><au>Nemati, F</au><au>Validire, P</au><au>Bellet, D</au><au>Bièche, I</au><au>Dangles-Marie, V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A short-term colorectal cancer sphere culture as a relevant tool for human cancer biology investigation</atitle><jtitle>British journal of cancer</jtitle><stitle>Br J Cancer</stitle><addtitle>Br J Cancer</addtitle><date>2013-04-30</date><risdate>2013</risdate><volume>108</volume><issue>8</issue><spage>1720</spage><epage>1731</epage><pages>1720-1731</pages><issn>0007-0920</issn><eissn>1532-1827</eissn><coden>BJCAAI</coden><abstract>Background:
Ex vivo
colospheres have been previously characterised as a colorectal cancer (CRC) well-rounded multicellular model, exclusively formed by carcinoma cells, and derived from fresh CRC tissue after mechanical dissociation. The ability to form colospheres was correlated with tumour aggressiveness. Their three-dimensional conformation prompted us to further investigate their potential interest as a preclinical cancer tool.
Methods:
Patient-derived CRC xenografts were used to produce numerous colospheres. Mechanism of formation was elucidated by confocal microscopy. Expression analysis of a panel of 64 selected cancer-related genes by real-time qRT–PCR and hierarchical clustering allowed comparison of colospheres with parent xenografts.
In vitro
and
in vivo
assays were performed for migration and chemosensitivity studies.
Results:
Colospheres, formed by tissue remodelling and compaction, remained viable several weeks in floating conditions, escaping anoikis through their strong cell–cell interactions. Colospheres matched the gene expression profile of the parent xenograft tissue. Colosphere-forming cells migrated in collagen I matrix and metastasised when subrenally implanted in
nude
mice. Besides, the colosphere responses to 5-fluorouracil and irinotecan, two standard drugs in CRC, reproduced those of the
in vivo
original xenografts.
Conclusion:
Colospheres closely mimic biological characteristics of
in vivo
CRC tumours. Consequently, they would be relevant
ex vivo
CRC models.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>23538387</pmid><doi>10.1038/bjc.2013.132</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | British journal of cancer, 2013-04, Vol.108 (8), p.1720-1731 |
| issn | 0007-0920 1532-1827 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals; Nature; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | 631/67/70 692/308 692/699/67/1504/1885 Animals Biological and medical sciences Biomedical and Life Sciences Biomedicine Camptothecin - analogs & derivatives Camptothecin - pharmacology Cancer Research Cancer therapies Cell Line, Tumor Cell Movement - physiology Cell Survival - physiology Colorectal cancer Colorectal Neoplasms - drug therapy Colorectal Neoplasms - genetics Colorectal Neoplasms - pathology Drug Resistance Drug Screening Assays, Antitumor Epidemiology Female Fluorouracil - pharmacology Gastroenterology. Liver. Pancreas. Abdomen Gene expression Humans Medical sciences Metastasis Mice Mice, Nude Mice, SCID Microscopy, Confocal Molecular Diagnostics Molecular Medicine Multiple tumors. Solid tumors. Tumors in childhood (general aspects) Neoplasm Transplantation Oncology Random Allocation Real-Time Polymerase Chain Reaction Spheroids, Cellular - pathology Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Transplantation, Heterologous Tumors Xenograft Model Antitumor Assays |
| title | A short-term colorectal cancer sphere culture as a relevant tool for human cancer biology investigation |
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