Characterization of Autologous Mesenchymal Stem Cell‐Derived Neural Progenitors as a Feasible Source of Stem Cells for Central Nervous System Applications in Multiple Sclerosis
Bone marrow mesenchymal stem cell‐derived neural progenitors (MSC‐NPs) are a potential therapeutic source of cells that have been shown to be efficacious in a preclinical model of multiple sclerosis (MS). To examine the feasibility of using MSC‐NPs as an autologous source of cells to promote central...
Gespeichert in:
| Veröffentlicht in: | Stem cells translational medicine 2012-07, Vol.1 (7), p.536-547 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 547 |
|---|---|
| container_issue | 7 |
| container_start_page | 536 |
| container_title | Stem cells translational medicine |
| container_volume | 1 |
| creator | Harris, Violaine K. Faroqui, Raihan Vyshkina, Tamara Sadiq, Saud A. |
| description | Bone marrow mesenchymal stem cell‐derived neural progenitors (MSC‐NPs) are a potential therapeutic source of cells that have been shown to be efficacious in a preclinical model of multiple sclerosis (MS). To examine the feasibility of using MSC‐NPs as an autologous source of cells to promote central nervous system (CNS) repair in MS, this study characterized human MSC‐NPs from a panel of both MS and non‐MS donors. Expanded MSCs showed similar characteristics in terms of growth and cell surface phenotype, regardless of the donor disease status. MSC‐NPs derived from all MSCs showed a consistent pattern of gene expression changes that correlated with neural commitment and increased homogeneity. Furthermore, the reduced expression of mesodermal markers and reduced capacity for adipogenic or osteogenic differentiation in MSC‐NPs compared with MSCs suggested that MSC‐NPs have reduced potential of unwanted mesodermal differentiation upon CNS transplantation. The immunoregulatory function of MSC‐NPs was similar to that of MSCs in their ability to suppress T‐cell proliferation and to promote expansion of FoxP3‐positive T regulatory cells in vitro. In addition, MSC‐NPs promoted oligodendroglial differentiation from brain‐derived neural stem cells that correlated with the secretion of bioactive factors. Our results provide a set of identity characteristics for autologous MSC‐NPs and suggest that the in vitro immunoregulatory and trophic properties of these cells may have therapeutic value in the treatment of MS.
To examine the feasibility of using mesenchymal stem cell‐derived neural progenitors (MSC‐NPs) as an autologous source of cells to promote central nervous system repair in multiple sclerosis (MS), this study characterized human MSC‐NPs from a panel of both MS and non‐MS donors. The results provide a set of identity characteristics for autologous MSC‐NPs and suggest that the in vitro immunoregulatory and trophic properties of these cells may have therapeutic value in the treatment of MS. |
| doi_str_mv | 10.5966/sctm.2012-0015 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_24P</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3659719</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1221129369</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4686-6bf433a8b51231abc1435711f023fcf03a6375e0225c0e1e4bf53dc184aff1683</originalsourceid><addsrcrecordid>eNqFUk1v1DAQjRCIVqVXjsgSFy5Z_BE7yQVpFSggtQVpy9lyvONdV0kc7GTRcuIn8Fv4SfwS7G5ZARcsSx5r3nueGb8se0rwgtdCvAx66hcUE5pjTPiD7JQSXuaCV_jhMRbFSXYewi2OS9SipvhxdkIZqcuKV6fZj2arvNITePtVTdYNyBm0nCfXuY2bA7qCAIPe7nvVodUEPWqg635--_46EnawRtcw-5j66N0GBjs5H5CKG12ACrbtAK3c7DUk1SM9ION8jIYpUa_B79JLq31IgOU4dlbflRKQHdDV3E12TEK6A--CDU-yR0Z1Ac7vz7Ps08Wbm-Zdfvnh7ftmeZnrQlQiF60pGFNVy0lsV7WaFIyXhBhMmdEGMyVYyQFTyjUGAkVrOFtrUhXKGCIqdpa9OuiOc9vDWh_qlaO3vfJ76ZSVf2cGu5Ubt5NM8LokdRR4cS_g3ecZwiR7G3QcgBogdiwJpYTQmokEff4P9DbObYjtSUprTDCr7gQXB5SOgwgezLEYgmVyhEyOkMkRMjkiEp792cIR_vv_I6A-AL7YDvb_kZOr5oalGyk5E-wXTKTH9w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2290103819</pqid></control><display><type>article</type><title>Characterization of Autologous Mesenchymal Stem Cell‐Derived Neural Progenitors as a Feasible Source of Stem Cells for Central Nervous System Applications in Multiple Sclerosis</title><source>Wiley Online Library Open Access</source><creator>Harris, Violaine K. ; Faroqui, Raihan ; Vyshkina, Tamara ; Sadiq, Saud A.</creator><creatorcontrib>Harris, Violaine K. ; Faroqui, Raihan ; Vyshkina, Tamara ; Sadiq, Saud A.</creatorcontrib><description>Bone marrow mesenchymal stem cell‐derived neural progenitors (MSC‐NPs) are a potential therapeutic source of cells that have been shown to be efficacious in a preclinical model of multiple sclerosis (MS). To examine the feasibility of using MSC‐NPs as an autologous source of cells to promote central nervous system (CNS) repair in MS, this study characterized human MSC‐NPs from a panel of both MS and non‐MS donors. Expanded MSCs showed similar characteristics in terms of growth and cell surface phenotype, regardless of the donor disease status. MSC‐NPs derived from all MSCs showed a consistent pattern of gene expression changes that correlated with neural commitment and increased homogeneity. Furthermore, the reduced expression of mesodermal markers and reduced capacity for adipogenic or osteogenic differentiation in MSC‐NPs compared with MSCs suggested that MSC‐NPs have reduced potential of unwanted mesodermal differentiation upon CNS transplantation. The immunoregulatory function of MSC‐NPs was similar to that of MSCs in their ability to suppress T‐cell proliferation and to promote expansion of FoxP3‐positive T regulatory cells in vitro. In addition, MSC‐NPs promoted oligodendroglial differentiation from brain‐derived neural stem cells that correlated with the secretion of bioactive factors. Our results provide a set of identity characteristics for autologous MSC‐NPs and suggest that the in vitro immunoregulatory and trophic properties of these cells may have therapeutic value in the treatment of MS.
To examine the feasibility of using mesenchymal stem cell‐derived neural progenitors (MSC‐NPs) as an autologous source of cells to promote central nervous system repair in multiple sclerosis (MS), this study characterized human MSC‐NPs from a panel of both MS and non‐MS donors. The results provide a set of identity characteristics for autologous MSC‐NPs and suggest that the in vitro immunoregulatory and trophic properties of these cells may have therapeutic value in the treatment of MS.</description><identifier>ISSN: 2157-6564</identifier><identifier>EISSN: 2157-6580</identifier><identifier>DOI: 10.5966/sctm.2012-0015</identifier><identifier>PMID: 23197858</identifier><language>eng</language><publisher>United States: AlphaMed Press</publisher><subject>Antigens, Differentiation - biosynthesis ; Antigens, Differentiation - immunology ; Autografts ; Autoimmune disease ; Autologous stem cell transplantation ; Bone marrow ; Brain stem ; Cell Proliferation ; Cell surface ; Cells, Cultured ; Central nervous system ; Clinical trials ; Data analysis ; Disease ; Female ; Forkhead Transcription Factors - metabolism ; Foxp3 protein ; Gene expression ; Gene Expression Regulation - immunology ; Growth factors ; Humans ; Immunoregulation ; Lymphocytes T ; Male ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - immunology ; Mesenchymal Stromal Cells - metabolism ; Mesenchymal Stromal Cells - pathology ; Mesenchyme ; Multiple sclerosis ; Multiple Sclerosis - immunology ; Multiple Sclerosis - metabolism ; Multiple Sclerosis - pathology ; Multiple Sclerosis - therapy ; Nervous system ; Neural stem cells ; Neural Stem Cells - immunology ; Neural Stem Cells - metabolism ; Neural Stem Cells - pathology ; Phenotypes ; Progenitor cells ; Review boards ; Spinal cord ; Stem Cell Transplantation ; Stem cells ; Studies ; T-Lymphocytes, Regulatory - immunology ; T-Lymphocytes, Regulatory - metabolism ; T-Lymphocytes, Regulatory - pathology ; Tissue-Specific Progenitor and Stem Cells ; Transplantation ; Transplantation, Autologous</subject><ispartof>Stem cells translational medicine, 2012-07, Vol.1 (7), p.536-547</ispartof><rights>2012 AlphaMed Press</rights><rights>2012. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>AlphaMed Press 1066-5099/2012/$20.00/0 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4686-6bf433a8b51231abc1435711f023fcf03a6375e0225c0e1e4bf53dc184aff1683</citedby><cites>FETCH-LOGICAL-c4686-6bf433a8b51231abc1435711f023fcf03a6375e0225c0e1e4bf53dc184aff1683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659719/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659719/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,862,883,1414,11551,27913,27914,45563,45564,46041,46465,53780,53782</link.rule.ids><linktorsrc>$$Uhttps://onlinelibrary.wiley.com/doi/abs/10.5966%2Fsctm.2012-0015$$EView_record_in_Wiley-Blackwell$$FView_record_in_$$GWiley-Blackwell</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23197858$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harris, Violaine K.</creatorcontrib><creatorcontrib>Faroqui, Raihan</creatorcontrib><creatorcontrib>Vyshkina, Tamara</creatorcontrib><creatorcontrib>Sadiq, Saud A.</creatorcontrib><title>Characterization of Autologous Mesenchymal Stem Cell‐Derived Neural Progenitors as a Feasible Source of Stem Cells for Central Nervous System Applications in Multiple Sclerosis</title><title>Stem cells translational medicine</title><addtitle>Stem Cells Transl Med</addtitle><description>Bone marrow mesenchymal stem cell‐derived neural progenitors (MSC‐NPs) are a potential therapeutic source of cells that have been shown to be efficacious in a preclinical model of multiple sclerosis (MS). To examine the feasibility of using MSC‐NPs as an autologous source of cells to promote central nervous system (CNS) repair in MS, this study characterized human MSC‐NPs from a panel of both MS and non‐MS donors. Expanded MSCs showed similar characteristics in terms of growth and cell surface phenotype, regardless of the donor disease status. MSC‐NPs derived from all MSCs showed a consistent pattern of gene expression changes that correlated with neural commitment and increased homogeneity. Furthermore, the reduced expression of mesodermal markers and reduced capacity for adipogenic or osteogenic differentiation in MSC‐NPs compared with MSCs suggested that MSC‐NPs have reduced potential of unwanted mesodermal differentiation upon CNS transplantation. The immunoregulatory function of MSC‐NPs was similar to that of MSCs in their ability to suppress T‐cell proliferation and to promote expansion of FoxP3‐positive T regulatory cells in vitro. In addition, MSC‐NPs promoted oligodendroglial differentiation from brain‐derived neural stem cells that correlated with the secretion of bioactive factors. Our results provide a set of identity characteristics for autologous MSC‐NPs and suggest that the in vitro immunoregulatory and trophic properties of these cells may have therapeutic value in the treatment of MS.
To examine the feasibility of using mesenchymal stem cell‐derived neural progenitors (MSC‐NPs) as an autologous source of cells to promote central nervous system repair in multiple sclerosis (MS), this study characterized human MSC‐NPs from a panel of both MS and non‐MS donors. The results provide a set of identity characteristics for autologous MSC‐NPs and suggest that the in vitro immunoregulatory and trophic properties of these cells may have therapeutic value in the treatment of MS.</description><subject>Antigens, Differentiation - biosynthesis</subject><subject>Antigens, Differentiation - immunology</subject><subject>Autografts</subject><subject>Autoimmune disease</subject><subject>Autologous stem cell transplantation</subject><subject>Bone marrow</subject><subject>Brain stem</subject><subject>Cell Proliferation</subject><subject>Cell surface</subject><subject>Cells, Cultured</subject><subject>Central nervous system</subject><subject>Clinical trials</subject><subject>Data analysis</subject><subject>Disease</subject><subject>Female</subject><subject>Forkhead Transcription Factors - metabolism</subject><subject>Foxp3 protein</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - immunology</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Immunoregulation</subject><subject>Lymphocytes T</subject><subject>Male</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - immunology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mesenchymal Stromal Cells - pathology</subject><subject>Mesenchyme</subject><subject>Multiple sclerosis</subject><subject>Multiple Sclerosis - immunology</subject><subject>Multiple Sclerosis - metabolism</subject><subject>Multiple Sclerosis - pathology</subject><subject>Multiple Sclerosis - therapy</subject><subject>Nervous system</subject><subject>Neural stem cells</subject><subject>Neural Stem Cells - immunology</subject><subject>Neural Stem Cells - metabolism</subject><subject>Neural Stem Cells - pathology</subject><subject>Phenotypes</subject><subject>Progenitor cells</subject><subject>Review boards</subject><subject>Spinal cord</subject><subject>Stem Cell Transplantation</subject><subject>Stem cells</subject><subject>Studies</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>T-Lymphocytes, Regulatory - metabolism</subject><subject>T-Lymphocytes, Regulatory - pathology</subject><subject>Tissue-Specific Progenitor and Stem Cells</subject><subject>Transplantation</subject><subject>Transplantation, Autologous</subject><issn>2157-6564</issn><issn>2157-6580</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFUk1v1DAQjRCIVqVXjsgSFy5Z_BE7yQVpFSggtQVpy9lyvONdV0kc7GTRcuIn8Fv4SfwS7G5ZARcsSx5r3nueGb8se0rwgtdCvAx66hcUE5pjTPiD7JQSXuaCV_jhMRbFSXYewi2OS9SipvhxdkIZqcuKV6fZj2arvNITePtVTdYNyBm0nCfXuY2bA7qCAIPe7nvVodUEPWqg635--_46EnawRtcw-5j66N0GBjs5H5CKG12ACrbtAK3c7DUk1SM9ION8jIYpUa_B79JLq31IgOU4dlbflRKQHdDV3E12TEK6A--CDU-yR0Z1Ac7vz7Ps08Wbm-Zdfvnh7ftmeZnrQlQiF60pGFNVy0lsV7WaFIyXhBhMmdEGMyVYyQFTyjUGAkVrOFtrUhXKGCIqdpa9OuiOc9vDWh_qlaO3vfJ76ZSVf2cGu5Ubt5NM8LokdRR4cS_g3ecZwiR7G3QcgBogdiwJpYTQmokEff4P9DbObYjtSUprTDCr7gQXB5SOgwgezLEYgmVyhEyOkMkRMjkiEp792cIR_vv_I6A-AL7YDvb_kZOr5oalGyk5E-wXTKTH9w</recordid><startdate>201207</startdate><enddate>201207</enddate><creator>Harris, Violaine K.</creator><creator>Faroqui, Raihan</creator><creator>Vyshkina, Tamara</creator><creator>Sadiq, Saud A.</creator><general>AlphaMed Press</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201207</creationdate><title>Characterization of Autologous Mesenchymal Stem Cell‐Derived Neural Progenitors as a Feasible Source of Stem Cells for Central Nervous System Applications in Multiple Sclerosis</title><author>Harris, Violaine K. ; Faroqui, Raihan ; Vyshkina, Tamara ; Sadiq, Saud A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4686-6bf433a8b51231abc1435711f023fcf03a6375e0225c0e1e4bf53dc184aff1683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Antigens, Differentiation - biosynthesis</topic><topic>Antigens, Differentiation - immunology</topic><topic>Autografts</topic><topic>Autoimmune disease</topic><topic>Autologous stem cell transplantation</topic><topic>Bone marrow</topic><topic>Brain stem</topic><topic>Cell Proliferation</topic><topic>Cell surface</topic><topic>Cells, Cultured</topic><topic>Central nervous system</topic><topic>Clinical trials</topic><topic>Data analysis</topic><topic>Disease</topic><topic>Female</topic><topic>Forkhead Transcription Factors - metabolism</topic><topic>Foxp3 protein</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - immunology</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Immunoregulation</topic><topic>Lymphocytes T</topic><topic>Male</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - immunology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mesenchymal Stromal Cells - pathology</topic><topic>Mesenchyme</topic><topic>Multiple sclerosis</topic><topic>Multiple Sclerosis - immunology</topic><topic>Multiple Sclerosis - metabolism</topic><topic>Multiple Sclerosis - pathology</topic><topic>Multiple Sclerosis - therapy</topic><topic>Nervous system</topic><topic>Neural stem cells</topic><topic>Neural Stem Cells - immunology</topic><topic>Neural Stem Cells - metabolism</topic><topic>Neural Stem Cells - pathology</topic><topic>Phenotypes</topic><topic>Progenitor cells</topic><topic>Review boards</topic><topic>Spinal cord</topic><topic>Stem Cell Transplantation</topic><topic>Stem cells</topic><topic>Studies</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>T-Lymphocytes, Regulatory - metabolism</topic><topic>T-Lymphocytes, Regulatory - pathology</topic><topic>Tissue-Specific Progenitor and Stem Cells</topic><topic>Transplantation</topic><topic>Transplantation, Autologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harris, Violaine K.</creatorcontrib><creatorcontrib>Faroqui, Raihan</creatorcontrib><creatorcontrib>Vyshkina, Tamara</creatorcontrib><creatorcontrib>Sadiq, Saud A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Stem cells translational medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Harris, Violaine K.</au><au>Faroqui, Raihan</au><au>Vyshkina, Tamara</au><au>Sadiq, Saud A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Autologous Mesenchymal Stem Cell‐Derived Neural Progenitors as a Feasible Source of Stem Cells for Central Nervous System Applications in Multiple Sclerosis</atitle><jtitle>Stem cells translational medicine</jtitle><addtitle>Stem Cells Transl Med</addtitle><date>2012-07</date><risdate>2012</risdate><volume>1</volume><issue>7</issue><spage>536</spage><epage>547</epage><pages>536-547</pages><issn>2157-6564</issn><eissn>2157-6580</eissn><abstract>Bone marrow mesenchymal stem cell‐derived neural progenitors (MSC‐NPs) are a potential therapeutic source of cells that have been shown to be efficacious in a preclinical model of multiple sclerosis (MS). To examine the feasibility of using MSC‐NPs as an autologous source of cells to promote central nervous system (CNS) repair in MS, this study characterized human MSC‐NPs from a panel of both MS and non‐MS donors. Expanded MSCs showed similar characteristics in terms of growth and cell surface phenotype, regardless of the donor disease status. MSC‐NPs derived from all MSCs showed a consistent pattern of gene expression changes that correlated with neural commitment and increased homogeneity. Furthermore, the reduced expression of mesodermal markers and reduced capacity for adipogenic or osteogenic differentiation in MSC‐NPs compared with MSCs suggested that MSC‐NPs have reduced potential of unwanted mesodermal differentiation upon CNS transplantation. The immunoregulatory function of MSC‐NPs was similar to that of MSCs in their ability to suppress T‐cell proliferation and to promote expansion of FoxP3‐positive T regulatory cells in vitro. In addition, MSC‐NPs promoted oligodendroglial differentiation from brain‐derived neural stem cells that correlated with the secretion of bioactive factors. Our results provide a set of identity characteristics for autologous MSC‐NPs and suggest that the in vitro immunoregulatory and trophic properties of these cells may have therapeutic value in the treatment of MS.
To examine the feasibility of using mesenchymal stem cell‐derived neural progenitors (MSC‐NPs) as an autologous source of cells to promote central nervous system repair in multiple sclerosis (MS), this study characterized human MSC‐NPs from a panel of both MS and non‐MS donors. The results provide a set of identity characteristics for autologous MSC‐NPs and suggest that the in vitro immunoregulatory and trophic properties of these cells may have therapeutic value in the treatment of MS.</abstract><cop>United States</cop><pub>AlphaMed Press</pub><pmid>23197858</pmid><doi>10.5966/sctm.2012-0015</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | ISSN: 2157-6564 |
| ispartof | Stem cells translational medicine, 2012-07, Vol.1 (7), p.536-547 |
| issn | 2157-6564 2157-6580 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3659719 |
| source | Wiley Online Library Open Access |
| subjects | Antigens, Differentiation - biosynthesis Antigens, Differentiation - immunology Autografts Autoimmune disease Autologous stem cell transplantation Bone marrow Brain stem Cell Proliferation Cell surface Cells, Cultured Central nervous system Clinical trials Data analysis Disease Female Forkhead Transcription Factors - metabolism Foxp3 protein Gene expression Gene Expression Regulation - immunology Growth factors Humans Immunoregulation Lymphocytes T Male Mesenchymal stem cells Mesenchymal Stromal Cells - immunology Mesenchymal Stromal Cells - metabolism Mesenchymal Stromal Cells - pathology Mesenchyme Multiple sclerosis Multiple Sclerosis - immunology Multiple Sclerosis - metabolism Multiple Sclerosis - pathology Multiple Sclerosis - therapy Nervous system Neural stem cells Neural Stem Cells - immunology Neural Stem Cells - metabolism Neural Stem Cells - pathology Phenotypes Progenitor cells Review boards Spinal cord Stem Cell Transplantation Stem cells Studies T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - metabolism T-Lymphocytes, Regulatory - pathology Tissue-Specific Progenitor and Stem Cells Transplantation Transplantation, Autologous |
| title | Characterization of Autologous Mesenchymal Stem Cell‐Derived Neural Progenitors as a Feasible Source of Stem Cells for Central Nervous System Applications in Multiple Sclerosis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T09%3A34%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_24P&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20Autologous%20Mesenchymal%20Stem%20Cell%E2%80%90Derived%20Neural%20Progenitors%20as%20a%20Feasible%20Source%20of%20Stem%20Cells%20for%20Central%20Nervous%20System%20Applications%20in%20Multiple%20Sclerosis&rft.jtitle=Stem%20cells%20translational%20medicine&rft.au=Harris,%20Violaine%20K.&rft.date=2012-07&rft.volume=1&rft.issue=7&rft.spage=536&rft.epage=547&rft.pages=536-547&rft.issn=2157-6564&rft.eissn=2157-6580&rft_id=info:doi/10.5966/sctm.2012-0015&rft_dat=%3Cproquest_24P%3E1221129369%3C/proquest_24P%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2290103819&rft_id=info:pmid/23197858&rfr_iscdi=true |