Hepatic stellate cells increase in Toxoplasma gondii infection in mice
BACKGROUND: Toxoplasma gondii is a ubiquitous protozoan parasite that can infect humans and animals. The severity of toxoplasmosis varies according to the immune status of the individual, parasite strain, and host species. In mammalian species, it has been observed that severe lesions of acute toxop...
Gespeichert in:
| Veröffentlicht in: | Parasites & vectors 2013-05, Vol.6 (1), p.135-135, Article 135 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 135 |
|---|---|
| container_issue | 1 |
| container_start_page | 135 |
| container_title | Parasites & vectors |
| container_volume | 6 |
| creator | Atmaca, Hasan Tarık Gazyagcı, Aycan Nuriye Canpolat, Sıla Kul, Oguz |
| description | BACKGROUND: Toxoplasma gondii is a ubiquitous protozoan parasite that can infect humans and animals. The severity of toxoplasmosis varies according to the immune status of the individual, parasite strain, and host species. In mammalian species, it has been observed that severe lesions of acute toxoplasmosis form in visceral organs such as the liver, lung, and spleen. Some epidemiological studies have reported an association of T. gondii infection with liver cirrhosis. METHODS: Acute infection was induced in fifteen 30-day-old normal Swiss albino mice. The mice were infected by intraperitoneal inoculation of 5000 T. gondii RH strain tachyzoites. The mice were sacrificed in groups of 5 at 2, 4, and 6 days after inoculation. Another group of 5 mice were used as the controls. Anti-glial fibrillary acidic protein (GFAP) and anti-T. gondii antibodies were used to compare GFAP-immunoreactive cells and anti-T. gondii–immunopositive areas in the liver between the T. gondii-infected groups and the healthy controls, respectively. RESULTS: There was a significant correlation between the numbers of GFAP-positive hepatic stellate cells (HSCs) when they were compared with T. gondii antigen immunostaining (p |
| doi_str_mv | 10.1186/1756-3305-6-135 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3659022</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A534781650</galeid><sourcerecordid>A534781650</sourcerecordid><originalsourceid>FETCH-LOGICAL-b704t-331c41fc62490796e4490a1430aa0102943babb268c029da8d7fd75b3caf35e73</originalsourceid><addsrcrecordid>eNqFks9r1UAQx4MotlbP3jTgRQ9p93eSi1CLtYWCYNvzMtlM4pYk-8wmUv97J7z6eJGK7GE2M5_9ZvY7mySvOTvmvDAnPNcmk5LpzGRc6ifJ4S7zdG9_kLyI8Y4xw0ptnicHQholhC4Pk_ML3MDkXRon7DqYMHUUY-oHNyJEpE16E-7DpoPYQ9qGofaekg26yYdhKffe4cvkWQNdxFcP8Si5Pf98c3aRXX39cnl2epVVOVMT9cKd4o0zQpUsLw0qisCVZACMM1EqWUFVCVM4-qihqPOmznUlHTRSYy6Pko9b3c1c9Vg7HKYROrsZfQ_jLxvA23Vl8N9tG35aaXTJhCCBT1uByod_CKwrLvR28dEuPlpjyWUSef_QxRh-zBgn2_u4-AYDhjlarjVdhiut_o_KsiyZ1oIT-u4v9C7M40B2Lj_Vksmi2KNa6NDSIAK16RZRe6qlygtuNCPq-BGKVo00rjBg4ym_OvBhdYCYCe-nFuYY7eX1tzV7smXdGGIcsdnZx5ldnuUjhr3ZH9uO__MOCXi7BRoIFtrRR3t7LRjXjLGiEGTkb7p14xI</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1355303881</pqid></control><display><type>article</type><title>Hepatic stellate cells increase in Toxoplasma gondii infection in mice</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>SpringerNature Journals</source><source>Springer Nature OA Free Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Atmaca, Hasan Tarık ; Gazyagcı, Aycan Nuriye ; Canpolat, Sıla ; Kul, Oguz</creator><creatorcontrib>Atmaca, Hasan Tarık ; Gazyagcı, Aycan Nuriye ; Canpolat, Sıla ; Kul, Oguz</creatorcontrib><description>BACKGROUND: Toxoplasma gondii is a ubiquitous protozoan parasite that can infect humans and animals. The severity of toxoplasmosis varies according to the immune status of the individual, parasite strain, and host species. In mammalian species, it has been observed that severe lesions of acute toxoplasmosis form in visceral organs such as the liver, lung, and spleen. Some epidemiological studies have reported an association of T. gondii infection with liver cirrhosis. METHODS: Acute infection was induced in fifteen 30-day-old normal Swiss albino mice. The mice were infected by intraperitoneal inoculation of 5000 T. gondii RH strain tachyzoites. The mice were sacrificed in groups of 5 at 2, 4, and 6 days after inoculation. Another group of 5 mice were used as the controls. Anti-glial fibrillary acidic protein (GFAP) and anti-T. gondii antibodies were used to compare GFAP-immunoreactive cells and anti-T. gondii–immunopositive areas in the liver between the T. gondii-infected groups and the healthy controls, respectively. RESULTS: There was a significant correlation between the numbers of GFAP-positive hepatic stellate cells (HSCs) when they were compared with T. gondii antigen immunostaining (p < 0.05). The amount of T. gondii immunostaining increased significantly with the increase in the number of HSCs. CONCLUSIONS: There is a significant relationship between the number of HSCs and T. gondii antigens, which may represent an active role of HSCs in liver pathology and the pathobiology of T. gondii-related hepatitis.</description><identifier>ISSN: 1756-3305</identifier><identifier>EISSN: 1756-3305</identifier><identifier>DOI: 10.1186/1756-3305-6-135</identifier><identifier>PMID: 23642259</identifier><language>eng</language><publisher>England: Springer-Verlag</publisher><subject>Animals ; Antibodies ; antigens ; Antigens, Protozoan - immunology ; Disease Models, Animal ; Drug therapy ; epidemiological studies ; Genetic aspects ; Health aspects ; Hepatic Stellate Cells ; hepatitis ; Hepatitis - parasitology ; Hepatitis - pathology ; humans ; Immunohistochemistry ; Infections ; Liver ; Liver - pathology ; Liver cirrhosis ; Mice ; Parasites ; Pathology ; Proteins ; spleen ; Studies ; tachyzoites ; Toxoplasma ; Toxoplasma - immunology ; Toxoplasma gondii ; Toxoplasmosis ; Toxoplasmosis - parasitology ; Toxoplasmosis - pathology ; Viral antibodies</subject><ispartof>Parasites & vectors, 2013-05, Vol.6 (1), p.135-135, Article 135</ispartof><rights>COPYRIGHT 2013 BioMed Central Ltd.</rights><rights>2013 Atmaca et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2013 Atmaca et al.; licensee BioMed Central Ltd. 2013 Atmaca et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b704t-331c41fc62490796e4490a1430aa0102943babb268c029da8d7fd75b3caf35e73</citedby><cites>FETCH-LOGICAL-b704t-331c41fc62490796e4490a1430aa0102943babb268c029da8d7fd75b3caf35e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659022/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659022/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23642259$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Atmaca, Hasan Tarık</creatorcontrib><creatorcontrib>Gazyagcı, Aycan Nuriye</creatorcontrib><creatorcontrib>Canpolat, Sıla</creatorcontrib><creatorcontrib>Kul, Oguz</creatorcontrib><title>Hepatic stellate cells increase in Toxoplasma gondii infection in mice</title><title>Parasites & vectors</title><addtitle>Parasit Vectors</addtitle><description>BACKGROUND: Toxoplasma gondii is a ubiquitous protozoan parasite that can infect humans and animals. The severity of toxoplasmosis varies according to the immune status of the individual, parasite strain, and host species. In mammalian species, it has been observed that severe lesions of acute toxoplasmosis form in visceral organs such as the liver, lung, and spleen. Some epidemiological studies have reported an association of T. gondii infection with liver cirrhosis. METHODS: Acute infection was induced in fifteen 30-day-old normal Swiss albino mice. The mice were infected by intraperitoneal inoculation of 5000 T. gondii RH strain tachyzoites. The mice were sacrificed in groups of 5 at 2, 4, and 6 days after inoculation. Another group of 5 mice were used as the controls. Anti-glial fibrillary acidic protein (GFAP) and anti-T. gondii antibodies were used to compare GFAP-immunoreactive cells and anti-T. gondii–immunopositive areas in the liver between the T. gondii-infected groups and the healthy controls, respectively. RESULTS: There was a significant correlation between the numbers of GFAP-positive hepatic stellate cells (HSCs) when they were compared with T. gondii antigen immunostaining (p < 0.05). The amount of T. gondii immunostaining increased significantly with the increase in the number of HSCs. CONCLUSIONS: There is a significant relationship between the number of HSCs and T. gondii antigens, which may represent an active role of HSCs in liver pathology and the pathobiology of T. gondii-related hepatitis.</description><subject>Animals</subject><subject>Antibodies</subject><subject>antigens</subject><subject>Antigens, Protozoan - immunology</subject><subject>Disease Models, Animal</subject><subject>Drug therapy</subject><subject>epidemiological studies</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Hepatic Stellate Cells</subject><subject>hepatitis</subject><subject>Hepatitis - parasitology</subject><subject>Hepatitis - pathology</subject><subject>humans</subject><subject>Immunohistochemistry</subject><subject>Infections</subject><subject>Liver</subject><subject>Liver - pathology</subject><subject>Liver cirrhosis</subject><subject>Mice</subject><subject>Parasites</subject><subject>Pathology</subject><subject>Proteins</subject><subject>spleen</subject><subject>Studies</subject><subject>tachyzoites</subject><subject>Toxoplasma</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis</subject><subject>Toxoplasmosis - parasitology</subject><subject>Toxoplasmosis - pathology</subject><subject>Viral antibodies</subject><issn>1756-3305</issn><issn>1756-3305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqFks9r1UAQx4MotlbP3jTgRQ9p93eSi1CLtYWCYNvzMtlM4pYk-8wmUv97J7z6eJGK7GE2M5_9ZvY7mySvOTvmvDAnPNcmk5LpzGRc6ifJ4S7zdG9_kLyI8Y4xw0ptnicHQholhC4Pk_ML3MDkXRon7DqYMHUUY-oHNyJEpE16E-7DpoPYQ9qGofaekg26yYdhKffe4cvkWQNdxFcP8Si5Pf98c3aRXX39cnl2epVVOVMT9cKd4o0zQpUsLw0qisCVZACMM1EqWUFVCVM4-qihqPOmznUlHTRSYy6Pko9b3c1c9Vg7HKYROrsZfQ_jLxvA23Vl8N9tG35aaXTJhCCBT1uByod_CKwrLvR28dEuPlpjyWUSef_QxRh-zBgn2_u4-AYDhjlarjVdhiut_o_KsiyZ1oIT-u4v9C7M40B2Lj_Vksmi2KNa6NDSIAK16RZRe6qlygtuNCPq-BGKVo00rjBg4ym_OvBhdYCYCe-nFuYY7eX1tzV7smXdGGIcsdnZx5ldnuUjhr3ZH9uO__MOCXi7BRoIFtrRR3t7LRjXjLGiEGTkb7p14xI</recordid><startdate>20130504</startdate><enddate>20130504</enddate><creator>Atmaca, Hasan Tarık</creator><creator>Gazyagcı, Aycan Nuriye</creator><creator>Canpolat, Sıla</creator><creator>Kul, Oguz</creator><general>Springer-Verlag</general><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H95</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130504</creationdate><title>Hepatic stellate cells increase in Toxoplasma gondii infection in mice</title><author>Atmaca, Hasan Tarık ; Gazyagcı, Aycan Nuriye ; Canpolat, Sıla ; Kul, Oguz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b704t-331c41fc62490796e4490a1430aa0102943babb268c029da8d7fd75b3caf35e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>antigens</topic><topic>Antigens, Protozoan - immunology</topic><topic>Disease Models, Animal</topic><topic>Drug therapy</topic><topic>epidemiological studies</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Hepatic Stellate Cells</topic><topic>hepatitis</topic><topic>Hepatitis - parasitology</topic><topic>Hepatitis - pathology</topic><topic>humans</topic><topic>Immunohistochemistry</topic><topic>Infections</topic><topic>Liver</topic><topic>Liver - pathology</topic><topic>Liver cirrhosis</topic><topic>Mice</topic><topic>Parasites</topic><topic>Pathology</topic><topic>Proteins</topic><topic>spleen</topic><topic>Studies</topic><topic>tachyzoites</topic><topic>Toxoplasma</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis</topic><topic>Toxoplasmosis - parasitology</topic><topic>Toxoplasmosis - pathology</topic><topic>Viral antibodies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Atmaca, Hasan Tarık</creatorcontrib><creatorcontrib>Gazyagcı, Aycan Nuriye</creatorcontrib><creatorcontrib>Canpolat, Sıla</creatorcontrib><creatorcontrib>Kul, Oguz</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Parasites & vectors</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Atmaca, Hasan Tarık</au><au>Gazyagcı, Aycan Nuriye</au><au>Canpolat, Sıla</au><au>Kul, Oguz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hepatic stellate cells increase in Toxoplasma gondii infection in mice</atitle><jtitle>Parasites & vectors</jtitle><addtitle>Parasit Vectors</addtitle><date>2013-05-04</date><risdate>2013</risdate><volume>6</volume><issue>1</issue><spage>135</spage><epage>135</epage><pages>135-135</pages><artnum>135</artnum><issn>1756-3305</issn><eissn>1756-3305</eissn><abstract>BACKGROUND: Toxoplasma gondii is a ubiquitous protozoan parasite that can infect humans and animals. The severity of toxoplasmosis varies according to the immune status of the individual, parasite strain, and host species. In mammalian species, it has been observed that severe lesions of acute toxoplasmosis form in visceral organs such as the liver, lung, and spleen. Some epidemiological studies have reported an association of T. gondii infection with liver cirrhosis. METHODS: Acute infection was induced in fifteen 30-day-old normal Swiss albino mice. The mice were infected by intraperitoneal inoculation of 5000 T. gondii RH strain tachyzoites. The mice were sacrificed in groups of 5 at 2, 4, and 6 days after inoculation. Another group of 5 mice were used as the controls. Anti-glial fibrillary acidic protein (GFAP) and anti-T. gondii antibodies were used to compare GFAP-immunoreactive cells and anti-T. gondii–immunopositive areas in the liver between the T. gondii-infected groups and the healthy controls, respectively. RESULTS: There was a significant correlation between the numbers of GFAP-positive hepatic stellate cells (HSCs) when they were compared with T. gondii antigen immunostaining (p < 0.05). The amount of T. gondii immunostaining increased significantly with the increase in the number of HSCs. CONCLUSIONS: There is a significant relationship between the number of HSCs and T. gondii antigens, which may represent an active role of HSCs in liver pathology and the pathobiology of T. gondii-related hepatitis.</abstract><cop>England</cop><pub>Springer-Verlag</pub><pmid>23642259</pmid><doi>10.1186/1756-3305-6-135</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1756-3305 |
| ispartof | Parasites & vectors, 2013-05, Vol.6 (1), p.135-135, Article 135 |
| issn | 1756-3305 1756-3305 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3659022 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; SpringerNature Journals; Springer Nature OA Free Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Antibodies antigens Antigens, Protozoan - immunology Disease Models, Animal Drug therapy epidemiological studies Genetic aspects Health aspects Hepatic Stellate Cells hepatitis Hepatitis - parasitology Hepatitis - pathology humans Immunohistochemistry Infections Liver Liver - pathology Liver cirrhosis Mice Parasites Pathology Proteins spleen Studies tachyzoites Toxoplasma Toxoplasma - immunology Toxoplasma gondii Toxoplasmosis Toxoplasmosis - parasitology Toxoplasmosis - pathology Viral antibodies |
| title | Hepatic stellate cells increase in Toxoplasma gondii infection in mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T22%3A30%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Hepatic%20stellate%20cells%20increase%20in%20Toxoplasma%20gondii%20infection%20in%20mice&rft.jtitle=Parasites%20&%20vectors&rft.au=Atmaca,%20Hasan%20Tar%C4%B1k&rft.date=2013-05-04&rft.volume=6&rft.issue=1&rft.spage=135&rft.epage=135&rft.pages=135-135&rft.artnum=135&rft.issn=1756-3305&rft.eissn=1756-3305&rft_id=info:doi/10.1186/1756-3305-6-135&rft_dat=%3Cgale_pubme%3EA534781650%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1355303881&rft_id=info:pmid/23642259&rft_galeid=A534781650&rfr_iscdi=true |