A dense network of dendritic cells populates the murine epididymis
One of the most intriguing aspects of male reproductive physiology is the ability to generate spermatogenic cells – which are ‘foreign’ to the host – without triggering immune activation. After leaving the testis, spermatozoa enter the epididymis where they mature and are stored. In this study, we r...
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| Veröffentlicht in: | Reproduction (Cambridge, England) England), 2011-05, Vol.141 (5), p.653-663 |
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| creator | Da Silva, Nicolas Cortez-Retamozo, Virna Reinecker, Hans-Christian Wildgruber, Moritz Hill, Eric Brown, Dennis Swirski, Filip K Pittet, Mikael J Breton, Sylvie |
| description | One of the most intriguing aspects of male reproductive physiology is the ability to generate spermatogenic cells – which are ‘foreign’ to the host – without triggering immune activation. After leaving the testis, spermatozoa enter the epididymis where they mature and are stored. In this study, we report a previously unrecognized dense network of dendritic cells (DCs) located at the base of the epididymal epithelium. This network was detected in transgenic mice expressing CD11c-EYFP and CX3CR1-GFP reporters. Epididymal DCs (eDCs) establish intimate interactions with the epithelium and project long dendrites between epithelial cells toward the lumen. We show that isolated eDCs express numerous leukocyte markers described previously in other organs that are in contact with the external environment, and present and cross-present ovalbumin to T cells in vitro. eDCs are, therefore, strategically positioned to regulate the complex interplay between immune tolerance and activation, a balance that is fundamental to male fertility. |
| doi_str_mv | 10.1530/REP-10-0493 |
| format | Article |
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We show that isolated eDCs express numerous leukocyte markers described previously in other organs that are in contact with the external environment, and present and cross-present ovalbumin to T cells in vitro. eDCs are, therefore, strategically positioned to regulate the complex interplay between immune tolerance and activation, a balance that is fundamental to male fertility.</description><identifier>ISSN: 1470-1626</identifier><identifier>EISSN: 1741-7899</identifier><identifier>DOI: 10.1530/REP-10-0493</identifier><identifier>PMID: 21310816</identifier><language>eng</language><publisher>England: BioScientifica</publisher><subject>Animals ; Antigen Presentation ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - genetics ; Biomarkers - metabolism ; CD11c Antigen - biosynthesis ; CD11c Antigen - genetics ; Cells, Cultured ; Coculture Techniques ; dendrites ; dendritic cells ; Dendritic Cells - immunology ; Dendritic Cells - metabolism ; epididymis ; Epididymis - cytology ; Epididymis - immunology ; Epididymis - metabolism ; epithelial cells ; epithelium ; Fertility ; Genes, Reporter ; Green Fluorescent Proteins - biosynthesis ; Green Fluorescent Proteins - genetics ; Immune Tolerance ; Immunophenotyping ; immunosuppression (physiological) ; Luminescent Proteins - biosynthesis ; Luminescent Proteins - genetics ; Macrophages - immunology ; Macrophages - metabolism ; Male ; male fertility ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Microscopy, Fluorescence ; ovalbumin ; Ovalbumin - immunology ; Phenotype ; Receptors, Chemokine - biosynthesis ; Receptors, Chemokine - genetics ; spermatozoa ; T-lymphocytes ; T-Lymphocytes - immunology ; testes</subject><ispartof>Reproduction (Cambridge, England), 2011-05, Vol.141 (5), p.653-663</ispartof><rights>2011 Society for Reproduction and Fertility</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b597t-67a5b4a12febbdcc98418f64bcb7cb16d24a83fc6f126e977802574920c5e9323</citedby><cites>FETCH-LOGICAL-b597t-67a5b4a12febbdcc98418f64bcb7cb16d24a83fc6f126e977802574920c5e9323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,782,786,887,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21310816$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Da Silva, Nicolas</creatorcontrib><creatorcontrib>Cortez-Retamozo, Virna</creatorcontrib><creatorcontrib>Reinecker, Hans-Christian</creatorcontrib><creatorcontrib>Wildgruber, Moritz</creatorcontrib><creatorcontrib>Hill, Eric</creatorcontrib><creatorcontrib>Brown, Dennis</creatorcontrib><creatorcontrib>Swirski, Filip K</creatorcontrib><creatorcontrib>Pittet, Mikael J</creatorcontrib><creatorcontrib>Breton, Sylvie</creatorcontrib><title>A dense network of dendritic cells populates the murine epididymis</title><title>Reproduction (Cambridge, England)</title><addtitle>Reproduction</addtitle><description>One of the most intriguing aspects of male reproductive physiology is the ability to generate spermatogenic cells – which are ‘foreign’ to the host – without triggering immune activation. 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Cortez-Retamozo, Virna ; Reinecker, Hans-Christian ; Wildgruber, Moritz ; Hill, Eric ; Brown, Dennis ; Swirski, Filip K ; Pittet, Mikael J ; Breton, Sylvie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b597t-67a5b4a12febbdcc98418f64bcb7cb16d24a83fc6f126e977802574920c5e9323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Antigen Presentation</topic><topic>Bacterial Proteins - biosynthesis</topic><topic>Bacterial Proteins - genetics</topic><topic>Biomarkers - metabolism</topic><topic>CD11c Antigen - biosynthesis</topic><topic>CD11c Antigen - genetics</topic><topic>Cells, Cultured</topic><topic>Coculture Techniques</topic><topic>dendrites</topic><topic>dendritic cells</topic><topic>Dendritic Cells - immunology</topic><topic>Dendritic Cells - metabolism</topic><topic>epididymis</topic><topic>Epididymis - cytology</topic><topic>Epididymis - immunology</topic><topic>Epididymis - metabolism</topic><topic>epithelial cells</topic><topic>epithelium</topic><topic>Fertility</topic><topic>Genes, Reporter</topic><topic>Green Fluorescent Proteins - biosynthesis</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Immune Tolerance</topic><topic>Immunophenotyping</topic><topic>immunosuppression (physiological)</topic><topic>Luminescent Proteins - biosynthesis</topic><topic>Luminescent Proteins - genetics</topic><topic>Macrophages - immunology</topic><topic>Macrophages - metabolism</topic><topic>Male</topic><topic>male fertility</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Microscopy, Fluorescence</topic><topic>ovalbumin</topic><topic>Ovalbumin - immunology</topic><topic>Phenotype</topic><topic>Receptors, Chemokine - biosynthesis</topic><topic>Receptors, Chemokine - genetics</topic><topic>spermatozoa</topic><topic>T-lymphocytes</topic><topic>T-Lymphocytes - immunology</topic><topic>testes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Da Silva, Nicolas</creatorcontrib><creatorcontrib>Cortez-Retamozo, Virna</creatorcontrib><creatorcontrib>Reinecker, Hans-Christian</creatorcontrib><creatorcontrib>Wildgruber, Moritz</creatorcontrib><creatorcontrib>Hill, Eric</creatorcontrib><creatorcontrib>Brown, Dennis</creatorcontrib><creatorcontrib>Swirski, Filip K</creatorcontrib><creatorcontrib>Pittet, Mikael J</creatorcontrib><creatorcontrib>Breton, Sylvie</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Reproduction (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Da Silva, Nicolas</au><au>Cortez-Retamozo, Virna</au><au>Reinecker, Hans-Christian</au><au>Wildgruber, Moritz</au><au>Hill, Eric</au><au>Brown, Dennis</au><au>Swirski, Filip K</au><au>Pittet, Mikael J</au><au>Breton, Sylvie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A dense network of dendritic cells populates the murine epididymis</atitle><jtitle>Reproduction (Cambridge, England)</jtitle><addtitle>Reproduction</addtitle><date>2011-05-01</date><risdate>2011</risdate><volume>141</volume><issue>5</issue><spage>653</spage><epage>663</epage><pages>653-663</pages><issn>1470-1626</issn><eissn>1741-7899</eissn><abstract>One of the most intriguing aspects of male reproductive physiology is the ability to generate spermatogenic cells – which are ‘foreign’ to the host – without triggering immune activation. After leaving the testis, spermatozoa enter the epididymis where they mature and are stored. In this study, we report a previously unrecognized dense network of dendritic cells (DCs) located at the base of the epididymal epithelium. This network was detected in transgenic mice expressing CD11c-EYFP and CX3CR1-GFP reporters. Epididymal DCs (eDCs) establish intimate interactions with the epithelium and project long dendrites between epithelial cells toward the lumen. We show that isolated eDCs express numerous leukocyte markers described previously in other organs that are in contact with the external environment, and present and cross-present ovalbumin to T cells in vitro. eDCs are, therefore, strategically positioned to regulate the complex interplay between immune tolerance and activation, a balance that is fundamental to male fertility.</abstract><cop>England</cop><pub>BioScientifica</pub><pmid>21310816</pmid><doi>10.1530/REP-10-0493</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Reproduction (Cambridge, England), 2011-05, Vol.141 (5), p.653-663 |
| issn | 1470-1626 1741-7899 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antigen Presentation Bacterial Proteins - biosynthesis Bacterial Proteins - genetics Biomarkers - metabolism CD11c Antigen - biosynthesis CD11c Antigen - genetics Cells, Cultured Coculture Techniques dendrites dendritic cells Dendritic Cells - immunology Dendritic Cells - metabolism epididymis Epididymis - cytology Epididymis - immunology Epididymis - metabolism epithelial cells epithelium Fertility Genes, Reporter Green Fluorescent Proteins - biosynthesis Green Fluorescent Proteins - genetics Immune Tolerance Immunophenotyping immunosuppression (physiological) Luminescent Proteins - biosynthesis Luminescent Proteins - genetics Macrophages - immunology Macrophages - metabolism Male male fertility Mice Mice, Inbred C57BL Mice, Transgenic Microscopy, Fluorescence ovalbumin Ovalbumin - immunology Phenotype Receptors, Chemokine - biosynthesis Receptors, Chemokine - genetics spermatozoa T-lymphocytes T-Lymphocytes - immunology testes |
| title | A dense network of dendritic cells populates the murine epididymis |
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