Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues
The relationship between the oxidative damage of nucleic acids and aging of animals was investigated by analyzing the nucleic acids derived from various tissue specimens of naturally aged Sprague-Dawley (SD) rats. For this purpose, we established an accurate and sensitive isotope-diluted LC-MS/MS me...
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| container_title | Oxidative medicine and cellular longevity |
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| creator | Cai, Jian-Ping Hayakawa, Hiroshi Chen, Lian-Guo Sekiguchi, Mutsuo Hu, Guo-Xin Shi, Fei Nie, Ben Gan, Wei |
| description | The relationship between the oxidative damage of nucleic acids and aging of animals was investigated by analyzing the nucleic acids derived from various tissue specimens of naturally aged Sprague-Dawley (SD) rats. For this purpose, we established an accurate and sensitive isotope-diluted LC-MS/MS method to determine the levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGsn) in DNA and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) in RNA. An age-dependent increase in oxidative DNA and RNA damage was observed in the various organs examined, including the brain, liver, kidneys, and testes. Similar increases in the 8-oxo-dGsn and 8-oxo-Gsn contents were observed in three parts of the brain, the hippocampus, cerebral cortex, and cerebellum, among which, the values for the hippocampus were always the highest. When the oxidized guanosine metabolites were quantified with urine, a similar age-dependent increase was observed for both 8-oxo-dGsn and 8-oxo-Gsn. However, unlike the results of nucleic acid samples derived from the tissues, the amount of 8-oxo-Gsn was significantly higher compared to that of 8-oxo-dGsn, probably reflecting the fact that RNA degradation occurs more frequently than DNA degradation. Our finding indicates that the amount of urinary 8-oxo-Gsn could be considered as a biomarker for the sensitive measurement of oxidative stress and aging. |
| doi_str_mv | 10.1155/2013/303181 |
| format | Article |
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For this purpose, we established an accurate and sensitive isotope-diluted LC-MS/MS method to determine the levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGsn) in DNA and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) in RNA. An age-dependent increase in oxidative DNA and RNA damage was observed in the various organs examined, including the brain, liver, kidneys, and testes. Similar increases in the 8-oxo-dGsn and 8-oxo-Gsn contents were observed in three parts of the brain, the hippocampus, cerebral cortex, and cerebellum, among which, the values for the hippocampus were always the highest. When the oxidized guanosine metabolites were quantified with urine, a similar age-dependent increase was observed for both 8-oxo-dGsn and 8-oxo-Gsn. However, unlike the results of nucleic acid samples derived from the tissues, the amount of 8-oxo-Gsn was significantly higher compared to that of 8-oxo-dGsn, probably reflecting the fact that RNA degradation occurs more frequently than DNA degradation. Our finding indicates that the amount of urinary 8-oxo-Gsn could be considered as a biomarker for the sensitive measurement of oxidative stress and aging.</description><identifier>ISSN: 1942-0900</identifier><identifier>EISSN: 1942-0994</identifier><identifier>DOI: 10.1155/2013/303181</identifier><identifier>PMID: 23738036</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>Aging - blood ; Aging - metabolism ; Aging - urine ; Animals ; Chromatography, High Pressure Liquid ; Deoxyguanosine - analogs & derivatives ; Deoxyguanosine - blood ; Deoxyguanosine - urine ; DNA - metabolism ; Guanine - analogs & derivatives ; Guanine - blood ; Guanine - metabolism ; Guanine - urine ; Male ; Mass Spectrometry ; Organ Specificity ; Oxidation-Reduction ; Rats ; Rats, Sprague-Dawley ; RNA - metabolism</subject><ispartof>Oxidative medicine and cellular longevity, 2013-01, Vol.2013 (2013), p.1-9</ispartof><rights>Copyright © 2013 Ben Nie et al.</rights><rights>Copyright © 2013 Ben Nie et al. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-91f6f5b6e291c8d15c9743343da3260b5b70834f0cdc22d33d8c56d6fae9c9af3</citedby><cites>FETCH-LOGICAL-c504t-91f6f5b6e291c8d15c9743343da3260b5b70834f0cdc22d33d8c56d6fae9c9af3</cites><orcidid>0000-0003-2793-9221</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657452/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657452/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23738036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Saretzki, Gabriele</contributor><creatorcontrib>Cai, Jian-Ping</creatorcontrib><creatorcontrib>Hayakawa, Hiroshi</creatorcontrib><creatorcontrib>Chen, Lian-Guo</creatorcontrib><creatorcontrib>Sekiguchi, Mutsuo</creatorcontrib><creatorcontrib>Hu, Guo-Xin</creatorcontrib><creatorcontrib>Shi, Fei</creatorcontrib><creatorcontrib>Nie, Ben</creatorcontrib><creatorcontrib>Gan, Wei</creatorcontrib><title>Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues</title><title>Oxidative medicine and cellular longevity</title><addtitle>Oxid Med Cell Longev</addtitle><description>The relationship between the oxidative damage of nucleic acids and aging of animals was investigated by analyzing the nucleic acids derived from various tissue specimens of naturally aged Sprague-Dawley (SD) rats. 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However, unlike the results of nucleic acid samples derived from the tissues, the amount of 8-oxo-Gsn was significantly higher compared to that of 8-oxo-dGsn, probably reflecting the fact that RNA degradation occurs more frequently than DNA degradation. Our finding indicates that the amount of urinary 8-oxo-Gsn could be considered as a biomarker for the sensitive measurement of oxidative stress and aging.</description><subject>Aging - blood</subject><subject>Aging - metabolism</subject><subject>Aging - urine</subject><subject>Animals</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Deoxyguanosine - analogs & derivatives</subject><subject>Deoxyguanosine - blood</subject><subject>Deoxyguanosine - urine</subject><subject>DNA - metabolism</subject><subject>Guanine - analogs & derivatives</subject><subject>Guanine - blood</subject><subject>Guanine - metabolism</subject><subject>Guanine - urine</subject><subject>Male</subject><subject>Mass Spectrometry</subject><subject>Organ Specificity</subject><subject>Oxidation-Reduction</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA - metabolism</subject><issn>1942-0900</issn><issn>1942-0994</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><recordid>eNqFkU1P3DAQhq0KVGDbU89FPiKqgO2xnfhSaQUtRUJFQtteLa8_do2yzjZOaPvvaxRYlROnGc08eufjRegDJWeUCnHOCIVzIEAb-gYdUsVZRZTie7uckAN0lPM9IRIYp2_RAYMaGgLyEC3mK19d-q1PzqcBz60dN2Nrhtgl3AXcVLd_utVoUkwex4SHtceX3-fYJIfvSiyln6aP3ZjxnRnwIuY8-vwO7QfTZv_-Kc7Qj69fFhffqpvbq-uL-U1lBeFDpWiQQSylZ4raxlFhVc0BODgDTJKlWNakAR6IdZYxB-AaK6STwXhllQkwQ58n3e243HhnywW9afW2jxvT_9WdifplJ8W1XnUPGqSouWBF4ORJoO9-lcUHvYnZ-rY1yZebNC2gagQp352hTxNq-y7n3ofdGEr0ow_60Qc9-VDo4_8327HPjy_A6QSsY3Lmd3xF7eME-4L4YHYwl7SWNfwDkqGYeA</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Cai, Jian-Ping</creator><creator>Hayakawa, Hiroshi</creator><creator>Chen, Lian-Guo</creator><creator>Sekiguchi, Mutsuo</creator><creator>Hu, Guo-Xin</creator><creator>Shi, Fei</creator><creator>Nie, Ben</creator><creator>Gan, Wei</creator><general>Hindawi Puplishing Corporation</general><general>Hindawi Publishing Corporation</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2793-9221</orcidid></search><sort><creationdate>20130101</creationdate><title>Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues</title><author>Cai, Jian-Ping ; Hayakawa, Hiroshi ; Chen, Lian-Guo ; Sekiguchi, Mutsuo ; Hu, Guo-Xin ; Shi, Fei ; Nie, Ben ; Gan, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-91f6f5b6e291c8d15c9743343da3260b5b70834f0cdc22d33d8c56d6fae9c9af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Aging - blood</topic><topic>Aging - metabolism</topic><topic>Aging - urine</topic><topic>Animals</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Deoxyguanosine - analogs & derivatives</topic><topic>Deoxyguanosine - blood</topic><topic>Deoxyguanosine - urine</topic><topic>DNA - metabolism</topic><topic>Guanine - analogs & derivatives</topic><topic>Guanine - blood</topic><topic>Guanine - metabolism</topic><topic>Guanine - urine</topic><topic>Male</topic><topic>Mass Spectrometry</topic><topic>Organ Specificity</topic><topic>Oxidation-Reduction</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cai, Jian-Ping</creatorcontrib><creatorcontrib>Hayakawa, Hiroshi</creatorcontrib><creatorcontrib>Chen, Lian-Guo</creatorcontrib><creatorcontrib>Sekiguchi, Mutsuo</creatorcontrib><creatorcontrib>Hu, Guo-Xin</creatorcontrib><creatorcontrib>Shi, Fei</creatorcontrib><creatorcontrib>Nie, Ben</creatorcontrib><creatorcontrib>Gan, Wei</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oxidative medicine and cellular longevity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cai, Jian-Ping</au><au>Hayakawa, Hiroshi</au><au>Chen, Lian-Guo</au><au>Sekiguchi, Mutsuo</au><au>Hu, Guo-Xin</au><au>Shi, Fei</au><au>Nie, Ben</au><au>Gan, Wei</au><au>Saretzki, Gabriele</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues</atitle><jtitle>Oxidative medicine and cellular longevity</jtitle><addtitle>Oxid Med Cell Longev</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>2013</volume><issue>2013</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>1942-0900</issn><eissn>1942-0994</eissn><abstract>The relationship between the oxidative damage of nucleic acids and aging of animals was investigated by analyzing the nucleic acids derived from various tissue specimens of naturally aged Sprague-Dawley (SD) rats. For this purpose, we established an accurate and sensitive isotope-diluted LC-MS/MS method to determine the levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGsn) in DNA and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) in RNA. An age-dependent increase in oxidative DNA and RNA damage was observed in the various organs examined, including the brain, liver, kidneys, and testes. Similar increases in the 8-oxo-dGsn and 8-oxo-Gsn contents were observed in three parts of the brain, the hippocampus, cerebral cortex, and cerebellum, among which, the values for the hippocampus were always the highest. When the oxidized guanosine metabolites were quantified with urine, a similar age-dependent increase was observed for both 8-oxo-dGsn and 8-oxo-Gsn. However, unlike the results of nucleic acid samples derived from the tissues, the amount of 8-oxo-Gsn was significantly higher compared to that of 8-oxo-dGsn, probably reflecting the fact that RNA degradation occurs more frequently than DNA degradation. Our finding indicates that the amount of urinary 8-oxo-Gsn could be considered as a biomarker for the sensitive measurement of oxidative stress and aging.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>23738036</pmid><doi>10.1155/2013/303181</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-2793-9221</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection |
| subjects | Aging - blood Aging - metabolism Aging - urine Animals Chromatography, High Pressure Liquid Deoxyguanosine - analogs & derivatives Deoxyguanosine - blood Deoxyguanosine - urine DNA - metabolism Guanine - analogs & derivatives Guanine - blood Guanine - metabolism Guanine - urine Male Mass Spectrometry Organ Specificity Oxidation-Reduction Rats Rats, Sprague-Dawley RNA - metabolism |
| title | Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues |
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