Solitary chemosensory cells and bitter taste receptor signaling in human sinonasal mucosa
Background Solitary chemosensory cells (SCCs) are specialized cells in the respiratory epithelium that respond to noxious chemicals including bacterial signaling molecules. SCCs express components of bitter taste transduction including the taste receptor type 2 (TAS2R) bitter taste receptors and dow...
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| Veröffentlicht in: | International forum of allergy & rhinology 2013-06, Vol.3 (6), p.450-457 |
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| description | Background
Solitary chemosensory cells (SCCs) are specialized cells in the respiratory epithelium that respond to noxious chemicals including bacterial signaling molecules. SCCs express components of bitter taste transduction including the taste receptor type 2 (TAS2R) bitter taste receptors and downstream signaling effectors: α‐Gustducin, phospholipase Cβ2 (PLCβ2), and transient receptor potential cation channel subfamily M member 5 (TRPM5). When activated, SCCs evoke neurogenic reflexes, resulting in local inflammation. The purpose of this study was to test for the presence SCCs in human sinonasal epithelium, and to test for a correlation with inflammatory disease processes such as allergic rhinitis and chronic rhinosinusitis.
Methods
Patient demographics and biopsies of human sinonasal mucosa were obtained from control patients (n = 7) and those with allergic rhinitis and/or chronic rhinosinusitis (n = 15). Reverse transcription polymerase chain reaction (RT‐PCR), quantitative PCR (qPCR), and immunohistochemistry were used to determine whether expression of signaling effectors was altered in diseased patients.
Results
RT‐PCR demonstrated that bitter taste receptors TAS2R4, TAS2R14, and TAS2R46, and downstream signaling effectors α‐Gustducin, PLCβ2, and TRPM5 are expressed in the inferior turbinate, middle turbinate, septum, and uncinate of both control and diseased patients. PLCβ2/TRPM5‐immunoreactive SCCs were identified in the sinonasal mucosa of both control and diseased patients. qPCR showed similar expression of α‐Gustducin and TRPM5 in the uncinate process of control and diseased groups, and there was no correlation between level of expression and 22‐item Sino‐Nasal Outcomes Test (SNOT‐22) or pain scores.
Conclusion
SCCs are present in human sinonasal mucosa in functionally relevant areas. Expression level of signaling effectors was similar in control and diseased patients and did not correlate with measures of pain and inflammation. Further study into these pathways may provide insight into nasal inflammatory diseases and may offer potential therapeutic targets. |
| doi_str_mv | 10.1002/alr.21149 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3655139</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3004061781</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5479-d2b21cddbac3bb466809de21ba2c1209078d08069b993d1d6143985180dbc2793</originalsourceid><addsrcrecordid>eNp1kV1PFDEUhhujEYJc-AdME2_0YqBf005vTAjBFVkwKsZ41fSL3eJMu7YzCv_eLgsbNbE37Wmf8-b0fQF4jtEBRogc6j4fEIyZfAR2CWKk4bJjj7dnwXfAfinXqK4Wty0WT8EOoQwxSbtd8O1z6sOo8y20Sz-k4mNJ68L3fYE6OmjCOPoMR11GD7O3fjWmDEtYRN2HuIAhwuU06FivYoq66B4Ok01FPwNPrnRf_P79vge-vD25PH7XzD_MTo-P5o1tmZCNI4Zg65zRlhrDOO-QdJ5go4nFBEkkOoc6xKWRkjrsOGZUdi3ukDOWCEn3wJuN7moyg3fWxzHrXq1yGOq3VNJB_f0Sw1It0k9FeXWDrgVe3Qvk9GPyZVRDKGsDdPRpKgpTQZAQnLKKvvwHvU5Trk7cUUiyVhJaqdcbyuZUSvZX22EwUuvMVM1M3WVW2Rd_Tr8lHxKqwOEG-BV6f_t_JXU0__Qg2Ww6Qo3sZtuh83fFBRWt-noxU2eXH9l7NDtT5_Q3tf2wYg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1370945923</pqid></control><display><type>article</type><title>Solitary chemosensory cells and bitter taste receptor signaling in human sinonasal mucosa</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Barham, Henry P. ; Cooper, Sarah E. ; Anderson, Catherine B. ; Tizzano, Marco ; Kingdom, Todd T. ; Finger, Tom E. ; Kinnamon, Sue C. ; Ramakrishnan, Vijay R.</creator><creatorcontrib>Barham, Henry P. ; Cooper, Sarah E. ; Anderson, Catherine B. ; Tizzano, Marco ; Kingdom, Todd T. ; Finger, Tom E. ; Kinnamon, Sue C. ; Ramakrishnan, Vijay R.</creatorcontrib><description>Background
Solitary chemosensory cells (SCCs) are specialized cells in the respiratory epithelium that respond to noxious chemicals including bacterial signaling molecules. SCCs express components of bitter taste transduction including the taste receptor type 2 (TAS2R) bitter taste receptors and downstream signaling effectors: α‐Gustducin, phospholipase Cβ2 (PLCβ2), and transient receptor potential cation channel subfamily M member 5 (TRPM5). When activated, SCCs evoke neurogenic reflexes, resulting in local inflammation. The purpose of this study was to test for the presence SCCs in human sinonasal epithelium, and to test for a correlation with inflammatory disease processes such as allergic rhinitis and chronic rhinosinusitis.
Methods
Patient demographics and biopsies of human sinonasal mucosa were obtained from control patients (n = 7) and those with allergic rhinitis and/or chronic rhinosinusitis (n = 15). Reverse transcription polymerase chain reaction (RT‐PCR), quantitative PCR (qPCR), and immunohistochemistry were used to determine whether expression of signaling effectors was altered in diseased patients.
Results
RT‐PCR demonstrated that bitter taste receptors TAS2R4, TAS2R14, and TAS2R46, and downstream signaling effectors α‐Gustducin, PLCβ2, and TRPM5 are expressed in the inferior turbinate, middle turbinate, septum, and uncinate of both control and diseased patients. PLCβ2/TRPM5‐immunoreactive SCCs were identified in the sinonasal mucosa of both control and diseased patients. qPCR showed similar expression of α‐Gustducin and TRPM5 in the uncinate process of control and diseased groups, and there was no correlation between level of expression and 22‐item Sino‐Nasal Outcomes Test (SNOT‐22) or pain scores.
Conclusion
SCCs are present in human sinonasal mucosa in functionally relevant areas. Expression level of signaling effectors was similar in control and diseased patients and did not correlate with measures of pain and inflammation. Further study into these pathways may provide insight into nasal inflammatory diseases and may offer potential therapeutic targets.</description><identifier>ISSN: 2042-6976</identifier><identifier>EISSN: 2042-6984</identifier><identifier>DOI: 10.1002/alr.21149</identifier><identifier>PMID: 23404938</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Adult ; Aged ; bitter taste transduction ; Case-Control Studies ; Chemoreceptor Cells - metabolism ; Chronic Disease ; Epithelium - metabolism ; Female ; Gene expression ; Humans ; inflammation ; Male ; Medical research ; Middle Aged ; Nasal Mucosa - metabolism ; Pain ; Phospholipase C beta - metabolism ; Polymerase Chain Reaction - methods ; Receptors, G-Protein-Coupled - metabolism ; respiratory epithelium ; rhinitis ; Rhinitis - metabolism ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial - metabolism ; rhinosinusitis ; Sinusitis - metabolism ; SNOT-22 ; solitary chemosensory cell ; Transducin - metabolism ; TRPM Cation Channels - metabolism</subject><ispartof>International forum of allergy & rhinology, 2013-06, Vol.3 (6), p.450-457</ispartof><rights>2013 ARS-AAOA, LLC</rights><rights>2013 ARS-AAOA, LLC.</rights><rights>Copyright © 2013 American Rhinologic Society-American Academy of Otolaryngic Allergy, LLC</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5479-d2b21cddbac3bb466809de21ba2c1209078d08069b993d1d6143985180dbc2793</citedby><cites>FETCH-LOGICAL-c5479-d2b21cddbac3bb466809de21ba2c1209078d08069b993d1d6143985180dbc2793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Falr.21149$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Falr.21149$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23404938$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barham, Henry P.</creatorcontrib><creatorcontrib>Cooper, Sarah E.</creatorcontrib><creatorcontrib>Anderson, Catherine B.</creatorcontrib><creatorcontrib>Tizzano, Marco</creatorcontrib><creatorcontrib>Kingdom, Todd T.</creatorcontrib><creatorcontrib>Finger, Tom E.</creatorcontrib><creatorcontrib>Kinnamon, Sue C.</creatorcontrib><creatorcontrib>Ramakrishnan, Vijay R.</creatorcontrib><title>Solitary chemosensory cells and bitter taste receptor signaling in human sinonasal mucosa</title><title>International forum of allergy & rhinology</title><addtitle>INTERNATIONAL FORUM OF ALLERGY AND RHINOLOGY</addtitle><description>Background
Solitary chemosensory cells (SCCs) are specialized cells in the respiratory epithelium that respond to noxious chemicals including bacterial signaling molecules. SCCs express components of bitter taste transduction including the taste receptor type 2 (TAS2R) bitter taste receptors and downstream signaling effectors: α‐Gustducin, phospholipase Cβ2 (PLCβ2), and transient receptor potential cation channel subfamily M member 5 (TRPM5). When activated, SCCs evoke neurogenic reflexes, resulting in local inflammation. The purpose of this study was to test for the presence SCCs in human sinonasal epithelium, and to test for a correlation with inflammatory disease processes such as allergic rhinitis and chronic rhinosinusitis.
Methods
Patient demographics and biopsies of human sinonasal mucosa were obtained from control patients (n = 7) and those with allergic rhinitis and/or chronic rhinosinusitis (n = 15). Reverse transcription polymerase chain reaction (RT‐PCR), quantitative PCR (qPCR), and immunohistochemistry were used to determine whether expression of signaling effectors was altered in diseased patients.
Results
RT‐PCR demonstrated that bitter taste receptors TAS2R4, TAS2R14, and TAS2R46, and downstream signaling effectors α‐Gustducin, PLCβ2, and TRPM5 are expressed in the inferior turbinate, middle turbinate, septum, and uncinate of both control and diseased patients. PLCβ2/TRPM5‐immunoreactive SCCs were identified in the sinonasal mucosa of both control and diseased patients. qPCR showed similar expression of α‐Gustducin and TRPM5 in the uncinate process of control and diseased groups, and there was no correlation between level of expression and 22‐item Sino‐Nasal Outcomes Test (SNOT‐22) or pain scores.
Conclusion
SCCs are present in human sinonasal mucosa in functionally relevant areas. Expression level of signaling effectors was similar in control and diseased patients and did not correlate with measures of pain and inflammation. Further study into these pathways may provide insight into nasal inflammatory diseases and may offer potential therapeutic targets.</description><subject>Adult</subject><subject>Aged</subject><subject>bitter taste transduction</subject><subject>Case-Control Studies</subject><subject>Chemoreceptor Cells - metabolism</subject><subject>Chronic Disease</subject><subject>Epithelium - metabolism</subject><subject>Female</subject><subject>Gene expression</subject><subject>Humans</subject><subject>inflammation</subject><subject>Male</subject><subject>Medical research</subject><subject>Middle Aged</subject><subject>Nasal Mucosa - metabolism</subject><subject>Pain</subject><subject>Phospholipase C beta - metabolism</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>respiratory epithelium</subject><subject>rhinitis</subject><subject>Rhinitis - metabolism</subject><subject>Rhinitis, Allergic</subject><subject>Rhinitis, Allergic, Perennial - metabolism</subject><subject>rhinosinusitis</subject><subject>Sinusitis - metabolism</subject><subject>SNOT-22</subject><subject>solitary chemosensory cell</subject><subject>Transducin - metabolism</subject><subject>TRPM Cation Channels - metabolism</subject><issn>2042-6976</issn><issn>2042-6984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kV1PFDEUhhujEYJc-AdME2_0YqBf005vTAjBFVkwKsZ41fSL3eJMu7YzCv_eLgsbNbE37Wmf8-b0fQF4jtEBRogc6j4fEIyZfAR2CWKk4bJjj7dnwXfAfinXqK4Wty0WT8EOoQwxSbtd8O1z6sOo8y20Sz-k4mNJ68L3fYE6OmjCOPoMR11GD7O3fjWmDEtYRN2HuIAhwuU06FivYoq66B4Ok01FPwNPrnRf_P79vge-vD25PH7XzD_MTo-P5o1tmZCNI4Zg65zRlhrDOO-QdJ5go4nFBEkkOoc6xKWRkjrsOGZUdi3ukDOWCEn3wJuN7moyg3fWxzHrXq1yGOq3VNJB_f0Sw1It0k9FeXWDrgVe3Qvk9GPyZVRDKGsDdPRpKgpTQZAQnLKKvvwHvU5Trk7cUUiyVhJaqdcbyuZUSvZX22EwUuvMVM1M3WVW2Rd_Tr8lHxKqwOEG-BV6f_t_JXU0__Qg2Ww6Qo3sZtuh83fFBRWt-noxU2eXH9l7NDtT5_Q3tf2wYg</recordid><startdate>201306</startdate><enddate>201306</enddate><creator>Barham, Henry P.</creator><creator>Cooper, Sarah E.</creator><creator>Anderson, Catherine B.</creator><creator>Tizzano, Marco</creator><creator>Kingdom, Todd T.</creator><creator>Finger, Tom E.</creator><creator>Kinnamon, Sue C.</creator><creator>Ramakrishnan, Vijay R.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201306</creationdate><title>Solitary chemosensory cells and bitter taste receptor signaling in human sinonasal mucosa</title><author>Barham, Henry P. ; Cooper, Sarah E. ; Anderson, Catherine B. ; Tizzano, Marco ; Kingdom, Todd T. ; Finger, Tom E. ; Kinnamon, Sue C. ; Ramakrishnan, Vijay R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5479-d2b21cddbac3bb466809de21ba2c1209078d08069b993d1d6143985180dbc2793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>bitter taste transduction</topic><topic>Case-Control Studies</topic><topic>Chemoreceptor Cells - metabolism</topic><topic>Chronic Disease</topic><topic>Epithelium - metabolism</topic><topic>Female</topic><topic>Gene expression</topic><topic>Humans</topic><topic>inflammation</topic><topic>Male</topic><topic>Medical research</topic><topic>Middle Aged</topic><topic>Nasal Mucosa - metabolism</topic><topic>Pain</topic><topic>Phospholipase C beta - metabolism</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>respiratory epithelium</topic><topic>rhinitis</topic><topic>Rhinitis - metabolism</topic><topic>Rhinitis, Allergic</topic><topic>Rhinitis, Allergic, Perennial - metabolism</topic><topic>rhinosinusitis</topic><topic>Sinusitis - metabolism</topic><topic>SNOT-22</topic><topic>solitary chemosensory cell</topic><topic>Transducin - metabolism</topic><topic>TRPM Cation Channels - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barham, Henry P.</creatorcontrib><creatorcontrib>Cooper, Sarah E.</creatorcontrib><creatorcontrib>Anderson, Catherine B.</creatorcontrib><creatorcontrib>Tizzano, Marco</creatorcontrib><creatorcontrib>Kingdom, Todd T.</creatorcontrib><creatorcontrib>Finger, Tom E.</creatorcontrib><creatorcontrib>Kinnamon, Sue C.</creatorcontrib><creatorcontrib>Ramakrishnan, Vijay R.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International forum of allergy & rhinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barham, Henry P.</au><au>Cooper, Sarah E.</au><au>Anderson, Catherine B.</au><au>Tizzano, Marco</au><au>Kingdom, Todd T.</au><au>Finger, Tom E.</au><au>Kinnamon, Sue C.</au><au>Ramakrishnan, Vijay R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Solitary chemosensory cells and bitter taste receptor signaling in human sinonasal mucosa</atitle><jtitle>International forum of allergy & rhinology</jtitle><addtitle>INTERNATIONAL FORUM OF ALLERGY AND RHINOLOGY</addtitle><date>2013-06</date><risdate>2013</risdate><volume>3</volume><issue>6</issue><spage>450</spage><epage>457</epage><pages>450-457</pages><issn>2042-6976</issn><eissn>2042-6984</eissn><abstract>Background
Solitary chemosensory cells (SCCs) are specialized cells in the respiratory epithelium that respond to noxious chemicals including bacterial signaling molecules. SCCs express components of bitter taste transduction including the taste receptor type 2 (TAS2R) bitter taste receptors and downstream signaling effectors: α‐Gustducin, phospholipase Cβ2 (PLCβ2), and transient receptor potential cation channel subfamily M member 5 (TRPM5). When activated, SCCs evoke neurogenic reflexes, resulting in local inflammation. The purpose of this study was to test for the presence SCCs in human sinonasal epithelium, and to test for a correlation with inflammatory disease processes such as allergic rhinitis and chronic rhinosinusitis.
Methods
Patient demographics and biopsies of human sinonasal mucosa were obtained from control patients (n = 7) and those with allergic rhinitis and/or chronic rhinosinusitis (n = 15). Reverse transcription polymerase chain reaction (RT‐PCR), quantitative PCR (qPCR), and immunohistochemistry were used to determine whether expression of signaling effectors was altered in diseased patients.
Results
RT‐PCR demonstrated that bitter taste receptors TAS2R4, TAS2R14, and TAS2R46, and downstream signaling effectors α‐Gustducin, PLCβ2, and TRPM5 are expressed in the inferior turbinate, middle turbinate, septum, and uncinate of both control and diseased patients. PLCβ2/TRPM5‐immunoreactive SCCs were identified in the sinonasal mucosa of both control and diseased patients. qPCR showed similar expression of α‐Gustducin and TRPM5 in the uncinate process of control and diseased groups, and there was no correlation between level of expression and 22‐item Sino‐Nasal Outcomes Test (SNOT‐22) or pain scores.
Conclusion
SCCs are present in human sinonasal mucosa in functionally relevant areas. Expression level of signaling effectors was similar in control and diseased patients and did not correlate with measures of pain and inflammation. Further study into these pathways may provide insight into nasal inflammatory diseases and may offer potential therapeutic targets.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>23404938</pmid><doi>10.1002/alr.21149</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adult Aged bitter taste transduction Case-Control Studies Chemoreceptor Cells - metabolism Chronic Disease Epithelium - metabolism Female Gene expression Humans inflammation Male Medical research Middle Aged Nasal Mucosa - metabolism Pain Phospholipase C beta - metabolism Polymerase Chain Reaction - methods Receptors, G-Protein-Coupled - metabolism respiratory epithelium rhinitis Rhinitis - metabolism Rhinitis, Allergic Rhinitis, Allergic, Perennial - metabolism rhinosinusitis Sinusitis - metabolism SNOT-22 solitary chemosensory cell Transducin - metabolism TRPM Cation Channels - metabolism |
| title | Solitary chemosensory cells and bitter taste receptor signaling in human sinonasal mucosa |
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