VPAC1 receptor expression in peripheral blood mononuclear cells in a human endotoxemia model
Vasoactive intestinal peptide (VIP) exerts immune-modulatory actions mainly via VPAC1 receptor stimulation. VPAC1 may be a treatment target of inflammatory diseases, but little is known about the receptor expression profile in immune-competent cells in vivo. 20 male healthy subjects received a singl...
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| Veröffentlicht in: | Journal of translational medicine 2013-05, Vol.11 (1), p.117-117, Article 117 |
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| creator | Storka, Angela Burian, Bernhard Führlinger, Gerhard Clive, Breanna Sun, Terri Crevenna, Richard Gsur, Andrea Mosgöller, Wilhelm Wolzt, Michael |
| description | Vasoactive intestinal peptide (VIP) exerts immune-modulatory actions mainly via VPAC1 receptor stimulation. VPAC1 may be a treatment target of inflammatory diseases, but little is known about the receptor expression profile in immune-competent cells in vivo.
20 male healthy subjects received a single intravenous bolus of 2 ng/kg body weight Escherichia coli endotoxin (LPS). Receptor status was evaluated in peripherial blood cells before and 3, 6 and 24 h after LPS by FACS analysis and q-PCR. VIP plasma concentrations were measured by ELISA.
Granulocytes accounted for 51% of leukocytes at baseline and 58 ± 37% were positive for VPAC1. The granulocyte population increased 2.6 fold after LPS, and a transient down-regulation of VPAC1 to 28 ± 23% was noted at 3 h (p |
| doi_str_mv | 10.1186/1479-5876-11-117 |
| format | Article |
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20 male healthy subjects received a single intravenous bolus of 2 ng/kg body weight Escherichia coli endotoxin (LPS). Receptor status was evaluated in peripherial blood cells before and 3, 6 and 24 h after LPS by FACS analysis and q-PCR. VIP plasma concentrations were measured by ELISA.
Granulocytes accounted for 51% of leukocytes at baseline and 58 ± 37% were positive for VPAC1. The granulocyte population increased 2.6 fold after LPS, and a transient down-regulation of VPAC1 to 28 ± 23% was noted at 3 h (p < 0.001), which returned to baseline at 24 hours. Baseline VPAC1 expression was low in lymphocytes (6.3 ± 3.2%) and monocytes (11 ± 9.6%). In these cells, LPS up-regulated VPAC1 at 6 h (13.2 ± 4.9%, p < 0.001) and 24 h (31.6 ± 20.5%, p = 0.001), respectively. Consistent changes were noted for the VIP-receptors VPAC2 and PAC1. VPAC1, VPAC2 and PAC1 mRNA levels were unchanged in peripheral blood mononuclear cells (PBMC). VIP plasma concentration increased from 0.5 ± 0.3 ng/ml to 0.7 ± 0.4 ng/ml at 6 h after LPS (p < 0.05) and returned to baseline within 24 h.
The time profile of VPAC receptor expression differs in granulocytes, monocytes and lymphocytes after LPS challenge in humans. Changes in circulating VIP concentrations may reflect innate immune responses.</description><identifier>ISSN: 1479-5876</identifier><identifier>EISSN: 1479-5876</identifier><identifier>DOI: 10.1186/1479-5876-11-117</identifier><identifier>PMID: 23651810</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adolescent ; Adult ; Blood ; Cell Separation ; Colleges & universities ; Complications and side effects ; Cytokines ; Development and progression ; E coli ; Endotoxemia - metabolism ; Escherichia coli ; Escherichia coli - metabolism ; Flow Cytometry ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation ; Genetic aspects ; Granulocytes - cytology ; Humans ; Inflammation ; Leukocytes, Mononuclear - cytology ; Lymphocytes ; Lymphocytes - cytology ; Male ; Middle Aged ; Monocytes - cytology ; Nitric oxide ; Patient outcomes ; Proteins ; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - metabolism ; Receptors, Vasoactive Intestinal Peptide, Type II - metabolism ; Receptors, Vasoactive Intestinal Polypeptide, Type I - genetics ; Receptors, Vasoactive Intestinal Polypeptide, Type I - metabolism ; Rheumatoid arthritis ; Rodents ; Time Factors ; Young Adult</subject><ispartof>Journal of translational medicine, 2013-05, Vol.11 (1), p.117-117, Article 117</ispartof><rights>COPYRIGHT 2013 BioMed Central Ltd.</rights><rights>2013 Storka et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2013 Storka et al.; licensee BioMed Central Ltd. 2013 Storka et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b584t-8dd2c034668cc5b1f00a3254844399af3e73573b45821a4f6d9c2f532ac574823</citedby><cites>FETCH-LOGICAL-b584t-8dd2c034668cc5b1f00a3254844399af3e73573b45821a4f6d9c2f532ac574823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651401/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651401/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23651810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Storka, Angela</creatorcontrib><creatorcontrib>Burian, Bernhard</creatorcontrib><creatorcontrib>Führlinger, Gerhard</creatorcontrib><creatorcontrib>Clive, Breanna</creatorcontrib><creatorcontrib>Sun, Terri</creatorcontrib><creatorcontrib>Crevenna, Richard</creatorcontrib><creatorcontrib>Gsur, Andrea</creatorcontrib><creatorcontrib>Mosgöller, Wilhelm</creatorcontrib><creatorcontrib>Wolzt, Michael</creatorcontrib><title>VPAC1 receptor expression in peripheral blood mononuclear cells in a human endotoxemia model</title><title>Journal of translational medicine</title><addtitle>J Transl Med</addtitle><description>Vasoactive intestinal peptide (VIP) exerts immune-modulatory actions mainly via VPAC1 receptor stimulation. VPAC1 may be a treatment target of inflammatory diseases, but little is known about the receptor expression profile in immune-competent cells in vivo.
20 male healthy subjects received a single intravenous bolus of 2 ng/kg body weight Escherichia coli endotoxin (LPS). Receptor status was evaluated in peripherial blood cells before and 3, 6 and 24 h after LPS by FACS analysis and q-PCR. VIP plasma concentrations were measured by ELISA.
Granulocytes accounted for 51% of leukocytes at baseline and 58 ± 37% were positive for VPAC1. The granulocyte population increased 2.6 fold after LPS, and a transient down-regulation of VPAC1 to 28 ± 23% was noted at 3 h (p < 0.001), which returned to baseline at 24 hours. Baseline VPAC1 expression was low in lymphocytes (6.3 ± 3.2%) and monocytes (11 ± 9.6%). In these cells, LPS up-regulated VPAC1 at 6 h (13.2 ± 4.9%, p < 0.001) and 24 h (31.6 ± 20.5%, p = 0.001), respectively. Consistent changes were noted for the VIP-receptors VPAC2 and PAC1. VPAC1, VPAC2 and PAC1 mRNA levels were unchanged in peripheral blood mononuclear cells (PBMC). VIP plasma concentration increased from 0.5 ± 0.3 ng/ml to 0.7 ± 0.4 ng/ml at 6 h after LPS (p < 0.05) and returned to baseline within 24 h.
The time profile of VPAC receptor expression differs in granulocytes, monocytes and lymphocytes after LPS challenge in humans. Changes in circulating VIP concentrations may reflect innate immune responses.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Blood</subject><subject>Cell Separation</subject><subject>Colleges & universities</subject><subject>Complications and side effects</subject><subject>Cytokines</subject><subject>Development and progression</subject><subject>E coli</subject><subject>Endotoxemia - metabolism</subject><subject>Escherichia coli</subject><subject>Escherichia coli - metabolism</subject><subject>Flow Cytometry</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Genetic aspects</subject><subject>Granulocytes - cytology</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Lymphocytes</subject><subject>Lymphocytes - cytology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Monocytes - cytology</subject><subject>Nitric oxide</subject><subject>Patient outcomes</subject><subject>Proteins</subject><subject>Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - metabolism</subject><subject>Receptors, Vasoactive Intestinal Peptide, Type II - metabolism</subject><subject>Receptors, Vasoactive Intestinal Polypeptide, Type I - genetics</subject><subject>Receptors, Vasoactive Intestinal Polypeptide, Type I - metabolism</subject><subject>Rheumatoid arthritis</subject><subject>Rodents</subject><subject>Time Factors</subject><subject>Young Adult</subject><issn>1479-5876</issn><issn>1479-5876</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkk2LFDEQhoMo7rp69yQNXrz0ms9O-iIMg1-woAf1JIR0unonS3fSJt3L-u9NmHXckRUkBQlVT70Ubwqh5wSfE6Ka14TLthZKNjUhOeQDdHpIPbzzPkFPUrrCmHLB28fohLJGEEXwKfr-7fNmS6oIFuYlxApu5ggpueAr56sZopt3EM1YdWMIfTUFH_xqRzCxsjCOqVCm2q2T8RX4PizhBiZnMtjD-BQ9GsyY4NntfYa-vnv7Zfuhvvj0_uN2c1F3QvGlVn1PLWa8aZS1oiMDxoZRwRXnrG3NwEAyIVnHhaLE8KHpW0sHwaixQnJF2Rl6s9ed126C3oJf8sh6jm4y8acOxunjinc7fRmudbGBY5IFtnuBzoV_CBxXbJh0cVcXdzUhOWRWeXU7Rgw_VkiLnlwqLhkPYU2asCaPKxui_gMVWLUU46L68i_0KqzRZz8LxTCRkoo_1KUZQTs_hDynLaJ6I7K1MouxTJ3fQ-XT50-zwcPgcv6oAe8bbAwpRRgOnhCsywre58KLu59xaPi9c-wXAPDU1w</recordid><startdate>20130507</startdate><enddate>20130507</enddate><creator>Storka, Angela</creator><creator>Burian, Bernhard</creator><creator>Führlinger, Gerhard</creator><creator>Clive, Breanna</creator><creator>Sun, Terri</creator><creator>Crevenna, Richard</creator><creator>Gsur, Andrea</creator><creator>Mosgöller, Wilhelm</creator><creator>Wolzt, Michael</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130507</creationdate><title>VPAC1 receptor expression in peripheral blood mononuclear cells in a human endotoxemia model</title><author>Storka, Angela ; Burian, Bernhard ; Führlinger, Gerhard ; Clive, Breanna ; Sun, Terri ; Crevenna, Richard ; Gsur, Andrea ; Mosgöller, Wilhelm ; Wolzt, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b584t-8dd2c034668cc5b1f00a3254844399af3e73573b45821a4f6d9c2f532ac574823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Blood</topic><topic>Cell Separation</topic><topic>Colleges & universities</topic><topic>Complications and side effects</topic><topic>Cytokines</topic><topic>Development and progression</topic><topic>E coli</topic><topic>Endotoxemia - metabolism</topic><topic>Escherichia coli</topic><topic>Escherichia coli - metabolism</topic><topic>Flow Cytometry</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Genetic aspects</topic><topic>Granulocytes - cytology</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Leukocytes, Mononuclear - cytology</topic><topic>Lymphocytes</topic><topic>Lymphocytes - cytology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Monocytes - cytology</topic><topic>Nitric oxide</topic><topic>Patient outcomes</topic><topic>Proteins</topic><topic>Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - metabolism</topic><topic>Receptors, Vasoactive Intestinal Peptide, Type II - metabolism</topic><topic>Receptors, Vasoactive Intestinal Polypeptide, Type I - genetics</topic><topic>Receptors, Vasoactive Intestinal Polypeptide, Type I - metabolism</topic><topic>Rheumatoid arthritis</topic><topic>Rodents</topic><topic>Time Factors</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Storka, Angela</creatorcontrib><creatorcontrib>Burian, Bernhard</creatorcontrib><creatorcontrib>Führlinger, Gerhard</creatorcontrib><creatorcontrib>Clive, Breanna</creatorcontrib><creatorcontrib>Sun, Terri</creatorcontrib><creatorcontrib>Crevenna, Richard</creatorcontrib><creatorcontrib>Gsur, Andrea</creatorcontrib><creatorcontrib>Mosgöller, Wilhelm</creatorcontrib><creatorcontrib>Wolzt, Michael</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of translational medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Storka, Angela</au><au>Burian, Bernhard</au><au>Führlinger, Gerhard</au><au>Clive, Breanna</au><au>Sun, Terri</au><au>Crevenna, Richard</au><au>Gsur, Andrea</au><au>Mosgöller, Wilhelm</au><au>Wolzt, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>VPAC1 receptor expression in peripheral blood mononuclear cells in a human endotoxemia model</atitle><jtitle>Journal of translational medicine</jtitle><addtitle>J Transl Med</addtitle><date>2013-05-07</date><risdate>2013</risdate><volume>11</volume><issue>1</issue><spage>117</spage><epage>117</epage><pages>117-117</pages><artnum>117</artnum><issn>1479-5876</issn><eissn>1479-5876</eissn><abstract>Vasoactive intestinal peptide (VIP) exerts immune-modulatory actions mainly via VPAC1 receptor stimulation. VPAC1 may be a treatment target of inflammatory diseases, but little is known about the receptor expression profile in immune-competent cells in vivo.
20 male healthy subjects received a single intravenous bolus of 2 ng/kg body weight Escherichia coli endotoxin (LPS). Receptor status was evaluated in peripherial blood cells before and 3, 6 and 24 h after LPS by FACS analysis and q-PCR. VIP plasma concentrations were measured by ELISA.
Granulocytes accounted for 51% of leukocytes at baseline and 58 ± 37% were positive for VPAC1. The granulocyte population increased 2.6 fold after LPS, and a transient down-regulation of VPAC1 to 28 ± 23% was noted at 3 h (p < 0.001), which returned to baseline at 24 hours. Baseline VPAC1 expression was low in lymphocytes (6.3 ± 3.2%) and monocytes (11 ± 9.6%). In these cells, LPS up-regulated VPAC1 at 6 h (13.2 ± 4.9%, p < 0.001) and 24 h (31.6 ± 20.5%, p = 0.001), respectively. Consistent changes were noted for the VIP-receptors VPAC2 and PAC1. VPAC1, VPAC2 and PAC1 mRNA levels were unchanged in peripheral blood mononuclear cells (PBMC). VIP plasma concentration increased from 0.5 ± 0.3 ng/ml to 0.7 ± 0.4 ng/ml at 6 h after LPS (p < 0.05) and returned to baseline within 24 h.
The time profile of VPAC receptor expression differs in granulocytes, monocytes and lymphocytes after LPS challenge in humans. Changes in circulating VIP concentrations may reflect innate immune responses.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>23651810</pmid><doi>10.1186/1479-5876-11-117</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; SpringerLink Journals - AutoHoldings; PubMed Central Open Access; Springer Nature OA Free Journals |
| subjects | Adolescent Adult Blood Cell Separation Colleges & universities Complications and side effects Cytokines Development and progression E coli Endotoxemia - metabolism Escherichia coli Escherichia coli - metabolism Flow Cytometry Gene expression Gene Expression Profiling Gene Expression Regulation Genetic aspects Granulocytes - cytology Humans Inflammation Leukocytes, Mononuclear - cytology Lymphocytes Lymphocytes - cytology Male Middle Aged Monocytes - cytology Nitric oxide Patient outcomes Proteins Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - metabolism Receptors, Vasoactive Intestinal Peptide, Type II - metabolism Receptors, Vasoactive Intestinal Polypeptide, Type I - genetics Receptors, Vasoactive Intestinal Polypeptide, Type I - metabolism Rheumatoid arthritis Rodents Time Factors Young Adult |
| title | VPAC1 receptor expression in peripheral blood mononuclear cells in a human endotoxemia model |
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