Involvement of the CD200 receptor complex in microglia activation in experimental glaucoma
The interaction of the myeloid restricted molecule CD200R with its widely expressed ligand CD200 is involved in the down-regulation of microglia activation. In the present study, we examined the involvement of CD200R in microglia activation in experimental ocular hypertension to determine the role o...
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| Veröffentlicht in: | Experimental eye research 2011-05, Vol.92 (5), p.338-343 |
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| creator | Taylor, Sarah Calder, Claudia J. Albon, Julie Erichsen, Jonathan T. Boulton, Micheal E. Morgan, James E. |
| description | The interaction of the myeloid restricted molecule CD200R with its widely expressed ligand CD200 is involved in the down-regulation of microglia activation. In the present study, we examined the involvement of CD200R in microglia activation in experimental ocular hypertension to determine the role of microglia activation in retinal ganglion cell (RGC) death, the key pathological event in glaucoma. Experimental glaucoma was induced in adult Brown Norway rats by sclerosis of the episcleral veins with the injection of hypertonic saline. Immunohistochemical methods were used to determine the involvement of microglia using GFAP, CD45, OX42 and OX41 and the involvement of CD200 and CD200R in the optic nerve head. Our data demonstrate the increased presence of microglia within the optic nerve head during ocular hypertension, identified by positive staining with OX42 and OX41. The peak of microglia correlates with peak in RGC death at days 20-27 (T3) post OHT induction. In addition, CD200 and CD200R positive cells were increased in ocular hypertensive eyes. Increased expression of CD200 was detected in the early phase (days 1-7; T1) of OHT and decreased over time, whilst the expression of CD200R was detected in the middle phase (days 20–27; T3) of OHT, correlating with the increase in microglia markers. Changes in the expression of CD200R/CD200 occur early in experimental glaucoma and precede the peak in microglia infiltration and RGC death, suggesting that CD200R-positive microglia play an important role in the initiation of RGC death during OHT, indicating a potential area for therapeutic intervention in treating glaucoma.
► CD200R is expressed on myeloid cells and is a marker of microglia activation. ► CD200R labelling increased in the optic nerve in experimental glaucoma, preceding the peak in retinal ganglion cell loss. ► Microglia activation may play a role in the initiation of RGC loss in experimental glaucoma. |
| doi_str_mv | 10.1016/j.exer.2011.01.012 |
| format | Article |
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► CD200R is expressed on myeloid cells and is a marker of microglia activation. ► CD200R labelling increased in the optic nerve in experimental glaucoma, preceding the peak in retinal ganglion cell loss. ► Microglia activation may play a role in the initiation of RGC loss in experimental glaucoma.</description><identifier>ISSN: 0014-4835</identifier><identifier>EISSN: 1096-0007</identifier><identifier>DOI: 10.1016/j.exer.2011.01.012</identifier><identifier>PMID: 21296076</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Biomarkers - metabolism ; CD200 ; CD200 antigen ; CD200R ; CD45 antigen ; Cell Death ; Data processing ; Disease Models, Animal ; experimental glaucoma ; Eye ; Fluorescent Antibody Technique, Indirect ; Glaucoma ; Glaucoma - metabolism ; Glial fibrillary acidic protein ; Glial Fibrillary Acidic Protein - metabolism ; Hypertension ; Leukocyte Common Antigens - metabolism ; Male ; Microglia ; Microglia - metabolism ; Optic Disk - metabolism ; Optic nerve ; Rats ; Rats, Inbred BN ; Receptors, Immunologic - metabolism ; Retinal ganglion cells ; Retinal Ganglion Cells - metabolism ; Retinal Ganglion Cells - pathology ; Sclerosis ; Therapeutic applications ; Veins</subject><ispartof>Experimental eye research, 2011-05, Vol.92 (5), p.338-343</ispartof><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><rights>2011 Elsevier Ltd. All rights reserved. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-d36ab7ec5fbee49b0be77869cd8890b4611fdcccd682fb45b36eb6a9d8efe5d93</citedby><cites>FETCH-LOGICAL-c487t-d36ab7ec5fbee49b0be77869cd8890b4611fdcccd682fb45b36eb6a9d8efe5d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014483511000327$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21296076$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taylor, Sarah</creatorcontrib><creatorcontrib>Calder, Claudia J.</creatorcontrib><creatorcontrib>Albon, Julie</creatorcontrib><creatorcontrib>Erichsen, Jonathan T.</creatorcontrib><creatorcontrib>Boulton, Micheal E.</creatorcontrib><creatorcontrib>Morgan, James E.</creatorcontrib><title>Involvement of the CD200 receptor complex in microglia activation in experimental glaucoma</title><title>Experimental eye research</title><addtitle>Exp Eye Res</addtitle><description>The interaction of the myeloid restricted molecule CD200R with its widely expressed ligand CD200 is involved in the down-regulation of microglia activation. In the present study, we examined the involvement of CD200R in microglia activation in experimental ocular hypertension to determine the role of microglia activation in retinal ganglion cell (RGC) death, the key pathological event in glaucoma. Experimental glaucoma was induced in adult Brown Norway rats by sclerosis of the episcleral veins with the injection of hypertonic saline. Immunohistochemical methods were used to determine the involvement of microglia using GFAP, CD45, OX42 and OX41 and the involvement of CD200 and CD200R in the optic nerve head. Our data demonstrate the increased presence of microglia within the optic nerve head during ocular hypertension, identified by positive staining with OX42 and OX41. The peak of microglia correlates with peak in RGC death at days 20-27 (T3) post OHT induction. In addition, CD200 and CD200R positive cells were increased in ocular hypertensive eyes. Increased expression of CD200 was detected in the early phase (days 1-7; T1) of OHT and decreased over time, whilst the expression of CD200R was detected in the middle phase (days 20–27; T3) of OHT, correlating with the increase in microglia markers. Changes in the expression of CD200R/CD200 occur early in experimental glaucoma and precede the peak in microglia infiltration and RGC death, suggesting that CD200R-positive microglia play an important role in the initiation of RGC death during OHT, indicating a potential area for therapeutic intervention in treating glaucoma.
► CD200R is expressed on myeloid cells and is a marker of microglia activation. ► CD200R labelling increased in the optic nerve in experimental glaucoma, preceding the peak in retinal ganglion cell loss. ► Microglia activation may play a role in the initiation of RGC loss in experimental glaucoma.</description><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>CD200</subject><subject>CD200 antigen</subject><subject>CD200R</subject><subject>CD45 antigen</subject><subject>Cell Death</subject><subject>Data processing</subject><subject>Disease Models, Animal</subject><subject>experimental glaucoma</subject><subject>Eye</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Glaucoma</subject><subject>Glaucoma - metabolism</subject><subject>Glial fibrillary acidic protein</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Hypertension</subject><subject>Leukocyte Common Antigens - metabolism</subject><subject>Male</subject><subject>Microglia</subject><subject>Microglia - metabolism</subject><subject>Optic Disk - metabolism</subject><subject>Optic nerve</subject><subject>Rats</subject><subject>Rats, Inbred BN</subject><subject>Receptors, Immunologic - metabolism</subject><subject>Retinal ganglion cells</subject><subject>Retinal Ganglion Cells - metabolism</subject><subject>Retinal Ganglion Cells - pathology</subject><subject>Sclerosis</subject><subject>Therapeutic applications</subject><subject>Veins</subject><issn>0014-4835</issn><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UV2L1DAUDaK4s6t_wAfpm750vPlomoIIMrq6sOCLvvgS0vR2NkPa1KRTxn9vyqyLvixcCNycczj3HEJeUdhSoPLdYYsnjFsGlG5hHfaEbCg0sgSA-inZAFBRCsWrC3KZ0iFvuajFc3LBKGsk1HJDft6MS_ALDjjOReiL-Q6L3ScGUES0OM0hFjYMk8dT4cZicDaGvXemMHZ2i5ldGNc9niaMbtUwvth7c8wc84I8641P-PL-vSI_rj9_330tb799udl9vC2tUPVcdlyatkZb9S2iaFposa6VbGynVAOtkJT2nbW2k4r1rahaLrGVpukU9lh1Db8iH86607EdsLPZRTReT9mQib91ME7__zO6O70Pi-ayAslVFnhzLxDDryOmWQ8uWfTejBiOSSvJBWuEqjLy7aPIHD4XkjFgGcrO0JxYShH7B0MU9FqfPui1Pr3Wp2GdlfT631MeKH_7yoD3ZwDmQBeX6ck6HC12Lvc16y64x_T_AI7TrpU</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>Taylor, Sarah</creator><creator>Calder, Claudia J.</creator><creator>Albon, Julie</creator><creator>Erichsen, Jonathan T.</creator><creator>Boulton, Micheal E.</creator><creator>Morgan, James E.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110501</creationdate><title>Involvement of the CD200 receptor complex in microglia activation in experimental glaucoma</title><author>Taylor, Sarah ; Calder, Claudia J. ; Albon, Julie ; Erichsen, Jonathan T. ; Boulton, Micheal E. ; Morgan, James E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-d36ab7ec5fbee49b0be77869cd8890b4611fdcccd682fb45b36eb6a9d8efe5d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biomarkers - metabolism</topic><topic>CD200</topic><topic>CD200 antigen</topic><topic>CD200R</topic><topic>CD45 antigen</topic><topic>Cell Death</topic><topic>Data processing</topic><topic>Disease Models, Animal</topic><topic>experimental glaucoma</topic><topic>Eye</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Glaucoma</topic><topic>Glaucoma - metabolism</topic><topic>Glial fibrillary acidic protein</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Hypertension</topic><topic>Leukocyte Common Antigens - metabolism</topic><topic>Male</topic><topic>Microglia</topic><topic>Microglia - metabolism</topic><topic>Optic Disk - metabolism</topic><topic>Optic nerve</topic><topic>Rats</topic><topic>Rats, Inbred BN</topic><topic>Receptors, Immunologic - metabolism</topic><topic>Retinal ganglion cells</topic><topic>Retinal Ganglion Cells - metabolism</topic><topic>Retinal Ganglion Cells - pathology</topic><topic>Sclerosis</topic><topic>Therapeutic applications</topic><topic>Veins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taylor, Sarah</creatorcontrib><creatorcontrib>Calder, Claudia J.</creatorcontrib><creatorcontrib>Albon, Julie</creatorcontrib><creatorcontrib>Erichsen, Jonathan T.</creatorcontrib><creatorcontrib>Boulton, Micheal E.</creatorcontrib><creatorcontrib>Morgan, James E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taylor, Sarah</au><au>Calder, Claudia J.</au><au>Albon, Julie</au><au>Erichsen, Jonathan T.</au><au>Boulton, Micheal E.</au><au>Morgan, James E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of the CD200 receptor complex in microglia activation in experimental glaucoma</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>2011-05-01</date><risdate>2011</risdate><volume>92</volume><issue>5</issue><spage>338</spage><epage>343</epage><pages>338-343</pages><issn>0014-4835</issn><eissn>1096-0007</eissn><abstract>The interaction of the myeloid restricted molecule CD200R with its widely expressed ligand CD200 is involved in the down-regulation of microglia activation. In the present study, we examined the involvement of CD200R in microglia activation in experimental ocular hypertension to determine the role of microglia activation in retinal ganglion cell (RGC) death, the key pathological event in glaucoma. Experimental glaucoma was induced in adult Brown Norway rats by sclerosis of the episcleral veins with the injection of hypertonic saline. Immunohistochemical methods were used to determine the involvement of microglia using GFAP, CD45, OX42 and OX41 and the involvement of CD200 and CD200R in the optic nerve head. Our data demonstrate the increased presence of microglia within the optic nerve head during ocular hypertension, identified by positive staining with OX42 and OX41. The peak of microglia correlates with peak in RGC death at days 20-27 (T3) post OHT induction. In addition, CD200 and CD200R positive cells were increased in ocular hypertensive eyes. Increased expression of CD200 was detected in the early phase (days 1-7; T1) of OHT and decreased over time, whilst the expression of CD200R was detected in the middle phase (days 20–27; T3) of OHT, correlating with the increase in microglia markers. Changes in the expression of CD200R/CD200 occur early in experimental glaucoma and precede the peak in microglia infiltration and RGC death, suggesting that CD200R-positive microglia play an important role in the initiation of RGC death during OHT, indicating a potential area for therapeutic intervention in treating glaucoma.
► CD200R is expressed on myeloid cells and is a marker of microglia activation. ► CD200R labelling increased in the optic nerve in experimental glaucoma, preceding the peak in retinal ganglion cell loss. ► Microglia activation may play a role in the initiation of RGC loss in experimental glaucoma.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>21296076</pmid><doi>10.1016/j.exer.2011.01.012</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Experimental eye research, 2011-05, Vol.92 (5), p.338-343 |
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| subjects | Animals Biomarkers - metabolism CD200 CD200 antigen CD200R CD45 antigen Cell Death Data processing Disease Models, Animal experimental glaucoma Eye Fluorescent Antibody Technique, Indirect Glaucoma Glaucoma - metabolism Glial fibrillary acidic protein Glial Fibrillary Acidic Protein - metabolism Hypertension Leukocyte Common Antigens - metabolism Male Microglia Microglia - metabolism Optic Disk - metabolism Optic nerve Rats Rats, Inbred BN Receptors, Immunologic - metabolism Retinal ganglion cells Retinal Ganglion Cells - metabolism Retinal Ganglion Cells - pathology Sclerosis Therapeutic applications Veins |
| title | Involvement of the CD200 receptor complex in microglia activation in experimental glaucoma |
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