Cutaneous delivery of α-tocopherol and lipoic acid using microemulsions: influence of composition and charge
Objectives To assess whether the composition and charge of microemulsions affect their ability to simultaneously deliver α‐tocopherol and lipoic acid into viable skin layers. Methods α‐Tocopherol and lipoic acid were added (1.1 and 0.5% w/w, respectively) to decylglucoside‐based microemulsions conta...
Gespeichert in:
| Veröffentlicht in: | Journal of pharmacy and pharmacology 2013-06, Vol.65 (6), p.817-826 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 826 |
|---|---|
| container_issue | 6 |
| container_start_page | 817 |
| container_title | Journal of pharmacy and pharmacology |
| container_volume | 65 |
| creator | Cichewicz, Allie Pacleb, Chelsea Connors, Ashley Hass, Martha A. Lopes, Luciana B. |
| description | Objectives
To assess whether the composition and charge of microemulsions affect their ability to simultaneously deliver α‐tocopherol and lipoic acid into viable skin layers.
Methods
α‐Tocopherol and lipoic acid were added (1.1 and 0.5% w/w, respectively) to decylglucoside‐based microemulsions containing mono‐dicaprylin. Microemulsions containing surfactant : oil : water (w/w/w) at 60 : 30 : 10 (ME‐O) and 46 : 23 : 31 (ME‐W), as well as a cationic form of ME‐W containing 1% phytosphingosine (ME‐Wphy) were characterized, and their ability to disrupt the skin barrier and deliver the antioxidants in vitro in the skin was evaluated. Antioxidant activity in ME‐Wphy‐treated skin was assessed using the thiobarbituric acid‐reactive substances (TBARS) assay.
Key findings
The internal phase diameters of microemulsions ranged between 42 and 55 nm; phytosphingosine addition and pH adjustment to 5.0 increased zeta potential from −4.3 to +29.1 mV. ME‐O displayed w/o structure, whereas ME‐W and ME‐Wphy were consistent with o/w. Microemulsions affected skin electrical resistance and transepidermal water loss, but did not affect lipoic acid penetration. α‐Tocopherol delivery increased following the order ME‐O < ME‐W < ME‐Wphy. ME‐Wphy presented suitable short‐term stability. The antioxidants delivered by ME‐Wphy decreased TBARS cutaneous levels.
Conclusions
Even though microemulsion structure only affected tocopherol penetration, delivered levels of both antioxidants were sufficient for a decrease in TBARS, supporting their use for enhanced protection. |
| doi_str_mv | 10.1111/jphp.12045 |
| format | Article |
| fullrecord | <record><control><sourceid>wiley_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3648887</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>JPHP12045</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4585-76c1e1d7c07d8343afb31fc4fd4ce1ed611b99285589061d0ef6575079fe7c093</originalsourceid><addsrcrecordid>eNp9kMFu1DAURS0EokNhwwcgr5FS7Di2ExZIaASdolK6aFV2lsd-nnFJ4shO2s5n8SP9JjwdOoJNvXmLd--x3kHoLSVHNL8P18N6OKIlqfgzNMujLCTl9XM0I6QsC8YlO0CvUromhEghxEt0UDJRSSH5DHXzadQ9hClhC62_gbjBweH738UYTBjWEEOLdW9x64fgDdbGWzwl369w500M0E1t8qFPH7HvXTtBb2ALMKEbQvJjXj3UzVrHFbxGL5xuE7z5Ow_R5dcvF_NFcfrj-GT--bQwFa95IYWhQK00RNqaVUy7JaPOVM5WBihYQemyacqa87ohgloCTnDJiWwc5FLDDtGnHXeYlh1YA_0YdauG6DsdNypor_7f9H6tVuFGZS91XcsMeL8D5BNTiuD2XUrUVrraSlcP0nP43b-_7aOPlnOA7gK3voXNEyj17Xxx_ggtdh2fRrjbd3T8pYRkkqurs2N1JRj9vvhJ1Jz9AfPZoBA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Cutaneous delivery of α-tocopherol and lipoic acid using microemulsions: influence of composition and charge</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library All Journals</source><creator>Cichewicz, Allie ; Pacleb, Chelsea ; Connors, Ashley ; Hass, Martha A. ; Lopes, Luciana B.</creator><creatorcontrib>Cichewicz, Allie ; Pacleb, Chelsea ; Connors, Ashley ; Hass, Martha A. ; Lopes, Luciana B.</creatorcontrib><description>Objectives
To assess whether the composition and charge of microemulsions affect their ability to simultaneously deliver α‐tocopherol and lipoic acid into viable skin layers.
Methods
α‐Tocopherol and lipoic acid were added (1.1 and 0.5% w/w, respectively) to decylglucoside‐based microemulsions containing mono‐dicaprylin. Microemulsions containing surfactant : oil : water (w/w/w) at 60 : 30 : 10 (ME‐O) and 46 : 23 : 31 (ME‐W), as well as a cationic form of ME‐W containing 1% phytosphingosine (ME‐Wphy) were characterized, and their ability to disrupt the skin barrier and deliver the antioxidants in vitro in the skin was evaluated. Antioxidant activity in ME‐Wphy‐treated skin was assessed using the thiobarbituric acid‐reactive substances (TBARS) assay.
Key findings
The internal phase diameters of microemulsions ranged between 42 and 55 nm; phytosphingosine addition and pH adjustment to 5.0 increased zeta potential from −4.3 to +29.1 mV. ME‐O displayed w/o structure, whereas ME‐W and ME‐Wphy were consistent with o/w. Microemulsions affected skin electrical resistance and transepidermal water loss, but did not affect lipoic acid penetration. α‐Tocopherol delivery increased following the order ME‐O < ME‐W < ME‐Wphy. ME‐Wphy presented suitable short‐term stability. The antioxidants delivered by ME‐Wphy decreased TBARS cutaneous levels.
Conclusions
Even though microemulsion structure only affected tocopherol penetration, delivered levels of both antioxidants were sufficient for a decrease in TBARS, supporting their use for enhanced protection.</description><identifier>ISSN: 0022-3573</identifier><identifier>EISSN: 2042-7158</identifier><identifier>DOI: 10.1111/jphp.12045</identifier><identifier>PMID: 23647675</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject><![CDATA[Administration, Cutaneous ; alpha-Tocopherol - administration & dosage ; alpha-Tocopherol - chemistry ; Animals ; Antioxidants - administration & dosage ; Antioxidants - chemistry ; Chemistry, Pharmaceutical - methods ; cutaneous delivery ; Emulsions - administration & dosage ; Emulsions - chemistry ; Glucosides - administration & dosage ; Glucosides - chemistry ; Hydrogen-Ion Concentration ; lipoic acid ; medium chain monoglycerides ; microemulsions ; Oils - chemistry ; Permeability ; phytosphingosine ; Research Paper ; Skin - metabolism ; Skin Absorption ; Sphingosine - analogs & derivatives ; Sphingosine - chemistry ; Surface-Active Agents - chemistry ; Swine ; Thioctic Acid - administration & dosage ; Thioctic Acid - chemistry ; Water - chemistry ; α-tocopherol]]></subject><ispartof>Journal of pharmacy and pharmacology, 2013-06, Vol.65 (6), p.817-826</ispartof><rights>2013 Royal Pharmaceutical Society</rights><rights>2013 Royal Pharmaceutical Society.</rights><rights>2013 Royal Pharmaceutical Society 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4585-76c1e1d7c07d8343afb31fc4fd4ce1ed611b99285589061d0ef6575079fe7c093</citedby><cites>FETCH-LOGICAL-c4585-76c1e1d7c07d8343afb31fc4fd4ce1ed611b99285589061d0ef6575079fe7c093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjphp.12045$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjphp.12045$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23647675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cichewicz, Allie</creatorcontrib><creatorcontrib>Pacleb, Chelsea</creatorcontrib><creatorcontrib>Connors, Ashley</creatorcontrib><creatorcontrib>Hass, Martha A.</creatorcontrib><creatorcontrib>Lopes, Luciana B.</creatorcontrib><title>Cutaneous delivery of α-tocopherol and lipoic acid using microemulsions: influence of composition and charge</title><title>Journal of pharmacy and pharmacology</title><addtitle>J Pharm Pharmacol</addtitle><description>Objectives
To assess whether the composition and charge of microemulsions affect their ability to simultaneously deliver α‐tocopherol and lipoic acid into viable skin layers.
Methods
α‐Tocopherol and lipoic acid were added (1.1 and 0.5% w/w, respectively) to decylglucoside‐based microemulsions containing mono‐dicaprylin. Microemulsions containing surfactant : oil : water (w/w/w) at 60 : 30 : 10 (ME‐O) and 46 : 23 : 31 (ME‐W), as well as a cationic form of ME‐W containing 1% phytosphingosine (ME‐Wphy) were characterized, and their ability to disrupt the skin barrier and deliver the antioxidants in vitro in the skin was evaluated. Antioxidant activity in ME‐Wphy‐treated skin was assessed using the thiobarbituric acid‐reactive substances (TBARS) assay.
Key findings
The internal phase diameters of microemulsions ranged between 42 and 55 nm; phytosphingosine addition and pH adjustment to 5.0 increased zeta potential from −4.3 to +29.1 mV. ME‐O displayed w/o structure, whereas ME‐W and ME‐Wphy were consistent with o/w. Microemulsions affected skin electrical resistance and transepidermal water loss, but did not affect lipoic acid penetration. α‐Tocopherol delivery increased following the order ME‐O < ME‐W < ME‐Wphy. ME‐Wphy presented suitable short‐term stability. The antioxidants delivered by ME‐Wphy decreased TBARS cutaneous levels.
Conclusions
Even though microemulsion structure only affected tocopherol penetration, delivered levels of both antioxidants were sufficient for a decrease in TBARS, supporting their use for enhanced protection.</description><subject>Administration, Cutaneous</subject><subject>alpha-Tocopherol - administration & dosage</subject><subject>alpha-Tocopherol - chemistry</subject><subject>Animals</subject><subject>Antioxidants - administration & dosage</subject><subject>Antioxidants - chemistry</subject><subject>Chemistry, Pharmaceutical - methods</subject><subject>cutaneous delivery</subject><subject>Emulsions - administration & dosage</subject><subject>Emulsions - chemistry</subject><subject>Glucosides - administration & dosage</subject><subject>Glucosides - chemistry</subject><subject>Hydrogen-Ion Concentration</subject><subject>lipoic acid</subject><subject>medium chain monoglycerides</subject><subject>microemulsions</subject><subject>Oils - chemistry</subject><subject>Permeability</subject><subject>phytosphingosine</subject><subject>Research Paper</subject><subject>Skin - metabolism</subject><subject>Skin Absorption</subject><subject>Sphingosine - analogs & derivatives</subject><subject>Sphingosine - chemistry</subject><subject>Surface-Active Agents - chemistry</subject><subject>Swine</subject><subject>Thioctic Acid - administration & dosage</subject><subject>Thioctic Acid - chemistry</subject><subject>Water - chemistry</subject><subject>α-tocopherol</subject><issn>0022-3573</issn><issn>2042-7158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu1DAURS0EokNhwwcgr5FS7Di2ExZIaASdolK6aFV2lsd-nnFJ4shO2s5n8SP9JjwdOoJNvXmLd--x3kHoLSVHNL8P18N6OKIlqfgzNMujLCTl9XM0I6QsC8YlO0CvUromhEghxEt0UDJRSSH5DHXzadQ9hClhC62_gbjBweH738UYTBjWEEOLdW9x64fgDdbGWzwl369w500M0E1t8qFPH7HvXTtBb2ALMKEbQvJjXj3UzVrHFbxGL5xuE7z5Ow_R5dcvF_NFcfrj-GT--bQwFa95IYWhQK00RNqaVUy7JaPOVM5WBihYQemyacqa87ohgloCTnDJiWwc5FLDDtGnHXeYlh1YA_0YdauG6DsdNypor_7f9H6tVuFGZS91XcsMeL8D5BNTiuD2XUrUVrraSlcP0nP43b-_7aOPlnOA7gK3voXNEyj17Xxx_ggtdh2fRrjbd3T8pYRkkqurs2N1JRj9vvhJ1Jz9AfPZoBA</recordid><startdate>201306</startdate><enddate>201306</enddate><creator>Cichewicz, Allie</creator><creator>Pacleb, Chelsea</creator><creator>Connors, Ashley</creator><creator>Hass, Martha A.</creator><creator>Lopes, Luciana B.</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>201306</creationdate><title>Cutaneous delivery of α-tocopherol and lipoic acid using microemulsions: influence of composition and charge</title><author>Cichewicz, Allie ; Pacleb, Chelsea ; Connors, Ashley ; Hass, Martha A. ; Lopes, Luciana B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4585-76c1e1d7c07d8343afb31fc4fd4ce1ed611b99285589061d0ef6575079fe7c093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Administration, Cutaneous</topic><topic>alpha-Tocopherol - administration & dosage</topic><topic>alpha-Tocopherol - chemistry</topic><topic>Animals</topic><topic>Antioxidants - administration & dosage</topic><topic>Antioxidants - chemistry</topic><topic>Chemistry, Pharmaceutical - methods</topic><topic>cutaneous delivery</topic><topic>Emulsions - administration & dosage</topic><topic>Emulsions - chemistry</topic><topic>Glucosides - administration & dosage</topic><topic>Glucosides - chemistry</topic><topic>Hydrogen-Ion Concentration</topic><topic>lipoic acid</topic><topic>medium chain monoglycerides</topic><topic>microemulsions</topic><topic>Oils - chemistry</topic><topic>Permeability</topic><topic>phytosphingosine</topic><topic>Research Paper</topic><topic>Skin - metabolism</topic><topic>Skin Absorption</topic><topic>Sphingosine - analogs & derivatives</topic><topic>Sphingosine - chemistry</topic><topic>Surface-Active Agents - chemistry</topic><topic>Swine</topic><topic>Thioctic Acid - administration & dosage</topic><topic>Thioctic Acid - chemistry</topic><topic>Water - chemistry</topic><topic>α-tocopherol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cichewicz, Allie</creatorcontrib><creatorcontrib>Pacleb, Chelsea</creatorcontrib><creatorcontrib>Connors, Ashley</creatorcontrib><creatorcontrib>Hass, Martha A.</creatorcontrib><creatorcontrib>Lopes, Luciana B.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of pharmacy and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cichewicz, Allie</au><au>Pacleb, Chelsea</au><au>Connors, Ashley</au><au>Hass, Martha A.</au><au>Lopes, Luciana B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cutaneous delivery of α-tocopherol and lipoic acid using microemulsions: influence of composition and charge</atitle><jtitle>Journal of pharmacy and pharmacology</jtitle><addtitle>J Pharm Pharmacol</addtitle><date>2013-06</date><risdate>2013</risdate><volume>65</volume><issue>6</issue><spage>817</spage><epage>826</epage><pages>817-826</pages><issn>0022-3573</issn><eissn>2042-7158</eissn><abstract>Objectives
To assess whether the composition and charge of microemulsions affect their ability to simultaneously deliver α‐tocopherol and lipoic acid into viable skin layers.
Methods
α‐Tocopherol and lipoic acid were added (1.1 and 0.5% w/w, respectively) to decylglucoside‐based microemulsions containing mono‐dicaprylin. Microemulsions containing surfactant : oil : water (w/w/w) at 60 : 30 : 10 (ME‐O) and 46 : 23 : 31 (ME‐W), as well as a cationic form of ME‐W containing 1% phytosphingosine (ME‐Wphy) were characterized, and their ability to disrupt the skin barrier and deliver the antioxidants in vitro in the skin was evaluated. Antioxidant activity in ME‐Wphy‐treated skin was assessed using the thiobarbituric acid‐reactive substances (TBARS) assay.
Key findings
The internal phase diameters of microemulsions ranged between 42 and 55 nm; phytosphingosine addition and pH adjustment to 5.0 increased zeta potential from −4.3 to +29.1 mV. ME‐O displayed w/o structure, whereas ME‐W and ME‐Wphy were consistent with o/w. Microemulsions affected skin electrical resistance and transepidermal water loss, but did not affect lipoic acid penetration. α‐Tocopherol delivery increased following the order ME‐O < ME‐W < ME‐Wphy. ME‐Wphy presented suitable short‐term stability. The antioxidants delivered by ME‐Wphy decreased TBARS cutaneous levels.
Conclusions
Even though microemulsion structure only affected tocopherol penetration, delivered levels of both antioxidants were sufficient for a decrease in TBARS, supporting their use for enhanced protection.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>23647675</pmid><doi>10.1111/jphp.12045</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3573 |
| ispartof | Journal of pharmacy and pharmacology, 2013-06, Vol.65 (6), p.817-826 |
| issn | 0022-3573 2042-7158 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3648887 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals |
| subjects | Administration, Cutaneous alpha-Tocopherol - administration & dosage alpha-Tocopherol - chemistry Animals Antioxidants - administration & dosage Antioxidants - chemistry Chemistry, Pharmaceutical - methods cutaneous delivery Emulsions - administration & dosage Emulsions - chemistry Glucosides - administration & dosage Glucosides - chemistry Hydrogen-Ion Concentration lipoic acid medium chain monoglycerides microemulsions Oils - chemistry Permeability phytosphingosine Research Paper Skin - metabolism Skin Absorption Sphingosine - analogs & derivatives Sphingosine - chemistry Surface-Active Agents - chemistry Swine Thioctic Acid - administration & dosage Thioctic Acid - chemistry Water - chemistry α-tocopherol |
| title | Cutaneous delivery of α-tocopherol and lipoic acid using microemulsions: influence of composition and charge |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T07%3A57%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wiley_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cutaneous%20delivery%20of%20%CE%B1-tocopherol%20and%20lipoic%20acid%20using%20microemulsions:%20influence%20of%20composition%20and%20charge&rft.jtitle=Journal%20of%20pharmacy%20and%20pharmacology&rft.au=Cichewicz,%20Allie&rft.date=2013-06&rft.volume=65&rft.issue=6&rft.spage=817&rft.epage=826&rft.pages=817-826&rft.issn=0022-3573&rft.eissn=2042-7158&rft_id=info:doi/10.1111/jphp.12045&rft_dat=%3Cwiley_pubme%3EJPHP12045%3C/wiley_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/23647675&rfr_iscdi=true |