Gene expression profiling of the anti-glioma effect of Cilengitide
Cilengitide (EMD121974), an inhibitor of the adhesive function of integrins, demonstrated preclinical efficacy against malignant glioma. It is speculated that cilengitide can inhibit tumor growth, invasion, and angiogenesis. However, the effects of cilengitide on these processes have not been suffic...
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| creator | Onishi, Manabu Kurozumi, Kazuhiko Ichikawa, Tomotsugu Michiue, Hiroyuki Fujii, Kentaro Ishida, Joji Shimazu, Yosuke Chiocca, E Antonio Kaur, Balveen Date, Isao |
| description | Cilengitide (EMD121974), an inhibitor of the adhesive function of integrins, demonstrated preclinical efficacy against malignant glioma. It is speculated that cilengitide can inhibit tumor growth, invasion, and angiogenesis. However, the effects of cilengitide on these processes have not been sufficiently examined. In this study, we investigated the anti-glioma effect of cilengitide using DNA microarray analysis. U87ΔEGFR cells (human malignant glioma cell line) were used for this experiment. The cells were harvested after 16 h of cilengitide treatment, and mRNA was extracted. Gene expression and pathway analyses were performed using a DNA microarray (CodeLink™Human Whole Genome Bioarray). The expression of 265 genes was changed with cilengitide treatment. The expression of 214 genes was up-regulated by more than 4-fold and the expression of 51 genes was down-regulated by more than 4-fold compared to the controls. In pathway analysis, “apoptotic cleavage of cellular proteins” and “TNF receptor signaling pathway” were over-represented. Apoptotic-associated genes such as caspase 8 were up-regulated. Gene expression profiling revealed more detailed mechanism of the anti-glioma effect of cilengitide. Genes associated with apoptosis were over-represented following cilengitide treatment. |
| doi_str_mv | 10.1186/2193-1801-2-160 |
| format | Article |
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It is speculated that cilengitide can inhibit tumor growth, invasion, and angiogenesis. However, the effects of cilengitide on these processes have not been sufficiently examined. In this study, we investigated the anti-glioma effect of cilengitide using DNA microarray analysis. U87ΔEGFR cells (human malignant glioma cell line) were used for this experiment. The cells were harvested after 16 h of cilengitide treatment, and mRNA was extracted. Gene expression and pathway analyses were performed using a DNA microarray (CodeLink™Human Whole Genome Bioarray). The expression of 265 genes was changed with cilengitide treatment. The expression of 214 genes was up-regulated by more than 4-fold and the expression of 51 genes was down-regulated by more than 4-fold compared to the controls. In pathway analysis, “apoptotic cleavage of cellular proteins” and “TNF receptor signaling pathway” were over-represented. Apoptotic-associated genes such as caspase 8 were up-regulated. Gene expression profiling revealed more detailed mechanism of the anti-glioma effect of cilengitide. Genes associated with apoptosis were over-represented following cilengitide treatment.</description><identifier>ISSN: 2193-1801</identifier><identifier>EISSN: 2193-1801</identifier><identifier>DOI: 10.1186/2193-1801-2-160</identifier><identifier>PMID: 23667810</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Humanities and Social Sciences ; Medicine ; multidisciplinary ; Science ; Science (multidisciplinary)</subject><ispartof>SpringerPlus, 2013-04, Vol.2 (1), p.160-160, Article 160</ispartof><rights>Onishi et al.; licensee Springer. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>SpringerPlus is a copyright of Springer, 2013.</rights><rights>Onishi et al.; licensee Springer. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b631t-8f27eb691d1b4b495be59707f92f372bbb0b0121d69826bab3c3efb29ef6a8083</citedby><cites>FETCH-LOGICAL-b631t-8f27eb691d1b4b495be59707f92f372bbb0b0121d69826bab3c3efb29ef6a8083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647089/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647089/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,41099,42168,51554,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23667810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Onishi, Manabu</creatorcontrib><creatorcontrib>Kurozumi, Kazuhiko</creatorcontrib><creatorcontrib>Ichikawa, Tomotsugu</creatorcontrib><creatorcontrib>Michiue, Hiroyuki</creatorcontrib><creatorcontrib>Fujii, Kentaro</creatorcontrib><creatorcontrib>Ishida, Joji</creatorcontrib><creatorcontrib>Shimazu, Yosuke</creatorcontrib><creatorcontrib>Chiocca, E Antonio</creatorcontrib><creatorcontrib>Kaur, Balveen</creatorcontrib><creatorcontrib>Date, Isao</creatorcontrib><title>Gene expression profiling of the anti-glioma effect of Cilengitide</title><title>SpringerPlus</title><addtitle>SpringerPlus</addtitle><addtitle>Springerplus</addtitle><description>Cilengitide (EMD121974), an inhibitor of the adhesive function of integrins, demonstrated preclinical efficacy against malignant glioma. 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Gene expression profiling revealed more detailed mechanism of the anti-glioma effect of cilengitide. 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Gene expression profiling revealed more detailed mechanism of the anti-glioma effect of cilengitide. Genes associated with apoptosis were over-represented following cilengitide treatment.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>23667810</pmid><doi>10.1186/2193-1801-2-160</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| title | Gene expression profiling of the anti-glioma effect of Cilengitide |
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