Melatonin inhibits GnRH-1, GnRH-3 and GnRH receptor expression in the brain of the European Sea Bass, Dicentrarchus labrax
Several evidences supported the existence of melatonin effects on reproductive system in fish. In order to investigate whether melatonin is involved in the modulation of GnRH systems in the European sea bass, we have injected melatonin (0.5 µg/g body mass) in male specimens. The brain mRNA transcrip...
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| Veröffentlicht in: | International journal of molecular sciences 2013-04, Vol.14 (4), p.7603-7616 |
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| creator | Servili, Arianna Herrera-Pérez, Patricia Del Carmen Rendón, María Muñoz-Cueto, José Antonio |
| description | Several evidences supported the existence of melatonin effects on reproductive system in fish. In order to investigate whether melatonin is involved in the modulation of GnRH systems in the European sea bass, we have injected melatonin (0.5 µg/g body mass) in male specimens. The brain mRNA transcript levels of the three GnRH forms and the five GnRH receptors present in this species were determined by real time quantitative PCR. Our findings revealed day-night variations in the brain expression of GnRH-1, GnRH-3 and several GnRH receptors (dlGnRHR-II-1c, -2a), which exhibited higher transcript levels at mid-light compared to mid-dark phase of the photocycle. Moreover, an inhibitory effect of melatonin on the nocturnal expression of GnRH-1, GnRH-3, and GnRH receptors subtypes 1c, 2a and 2b was also demonstrated. Interestingly, the inhibitory effect of melatonin affected the expression of hypophysiotrophic GnRH forms and GnRH receptors that exhibit day-night fluctuations, suggesting that exogenous melatonin reinforce physiological mechanisms already established. These interactions between melatoninergic and GnRH systems could be mediating photoperiod effects on reproductive and other rhythmic physiological events in the European sea bass. |
| doi_str_mv | 10.3390/ijms14047603 |
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In order to investigate whether melatonin is involved in the modulation of GnRH systems in the European sea bass, we have injected melatonin (0.5 µg/g body mass) in male specimens. The brain mRNA transcript levels of the three GnRH forms and the five GnRH receptors present in this species were determined by real time quantitative PCR. Our findings revealed day-night variations in the brain expression of GnRH-1, GnRH-3 and several GnRH receptors (dlGnRHR-II-1c, -2a), which exhibited higher transcript levels at mid-light compared to mid-dark phase of the photocycle. Moreover, an inhibitory effect of melatonin on the nocturnal expression of GnRH-1, GnRH-3, and GnRH receptors subtypes 1c, 2a and 2b was also demonstrated. Interestingly, the inhibitory effect of melatonin affected the expression of hypophysiotrophic GnRH forms and GnRH receptors that exhibit day-night fluctuations, suggesting that exogenous melatonin reinforce physiological mechanisms already established. These interactions between melatoninergic and GnRH systems could be mediating photoperiod effects on reproductive and other rhythmic physiological events in the European sea bass.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms14047603</identifier><identifier>PMID: 23567273</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Animals ; Bass ; Bass - metabolism ; Binding sites ; Brain - metabolism ; College campuses ; Female ; Fish Proteins - biosynthesis ; Gene Expression Regulation - physiology ; Hormones ; Male ; Melatonin ; Melatonin - metabolism ; Photoperiod ; Physiology ; Pituitary gland ; Receptors, LHRH - biosynthesis ; Reproduction - physiology ; Seasonal variations</subject><ispartof>International journal of molecular sciences, 2013-04, Vol.14 (4), p.7603-7616</ispartof><rights>Copyright MDPI AG 2013</rights><rights>2013 by the authors; licensee MDPI, Basel, Switzerland 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-652abe7c59166978a3bb33822983b2ad1a15c482eb0e63b6d9e8e90d189694ef3</citedby><cites>FETCH-LOGICAL-c478t-652abe7c59166978a3bb33822983b2ad1a15c482eb0e63b6d9e8e90d189694ef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3645706/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3645706/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23567273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Servili, Arianna</creatorcontrib><creatorcontrib>Herrera-Pérez, Patricia</creatorcontrib><creatorcontrib>Del Carmen Rendón, María</creatorcontrib><creatorcontrib>Muñoz-Cueto, José Antonio</creatorcontrib><title>Melatonin inhibits GnRH-1, GnRH-3 and GnRH receptor expression in the brain of the European Sea Bass, Dicentrarchus labrax</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Several evidences supported the existence of melatonin effects on reproductive system in fish. In order to investigate whether melatonin is involved in the modulation of GnRH systems in the European sea bass, we have injected melatonin (0.5 µg/g body mass) in male specimens. The brain mRNA transcript levels of the three GnRH forms and the five GnRH receptors present in this species were determined by real time quantitative PCR. Our findings revealed day-night variations in the brain expression of GnRH-1, GnRH-3 and several GnRH receptors (dlGnRHR-II-1c, -2a), which exhibited higher transcript levels at mid-light compared to mid-dark phase of the photocycle. Moreover, an inhibitory effect of melatonin on the nocturnal expression of GnRH-1, GnRH-3, and GnRH receptors subtypes 1c, 2a and 2b was also demonstrated. Interestingly, the inhibitory effect of melatonin affected the expression of hypophysiotrophic GnRH forms and GnRH receptors that exhibit day-night fluctuations, suggesting that exogenous melatonin reinforce physiological mechanisms already established. These interactions between melatoninergic and GnRH systems could be mediating photoperiod effects on reproductive and other rhythmic physiological events in the European sea bass.</description><subject>Animals</subject><subject>Bass</subject><subject>Bass - metabolism</subject><subject>Binding sites</subject><subject>Brain - metabolism</subject><subject>College campuses</subject><subject>Female</subject><subject>Fish Proteins - biosynthesis</subject><subject>Gene Expression Regulation - physiology</subject><subject>Hormones</subject><subject>Male</subject><subject>Melatonin</subject><subject>Melatonin - metabolism</subject><subject>Photoperiod</subject><subject>Physiology</subject><subject>Pituitary gland</subject><subject>Receptors, LHRH - biosynthesis</subject><subject>Reproduction - physiology</subject><subject>Seasonal variations</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc1v1DAQxa0K1JbSW8_IEhcOG2p7bCe-VIJSWqRWSHycLTuZZb3K2sFOUOGvJ-2Wastp3si_eZrxI-SEs7cAhp2G9aZwyWStGeyRQy6FqBjT9bMdfUBelLJmTIBQZp8cCFC6FjUckj832LsxxRBpiKvgw1joZfxyVfHFtgJ1sbuXNGOLw5gyxdshYykh3Q3RcYXUZzertLxvLqacBnSRfkVH37tSFvRDaDGO2eV2NRXau5m_fUmeL11f8PihHpHvHy--nV9V158vP52_u65aWTdjpZVwHutWGa61qRsH3gM0QpgGvHAdd1y1shHoGWrwujPYoGEdb4w2EpdwRM62vsPkN9htF-ntkMPG5d82uWCfvsSwsj_SLwtaqprp2eDNg0FOPycso92E0mLfu4hpKpbPvwrAlRYz-vo_dJ2mHOfzLFdCMymkVjO12FJtTqVkXD4uw5m9C9Xuhjrjr3YPeIT_pQh_AbTInMg</recordid><startdate>20130408</startdate><enddate>20130408</enddate><creator>Servili, Arianna</creator><creator>Herrera-Pérez, Patricia</creator><creator>Del Carmen Rendón, María</creator><creator>Muñoz-Cueto, José Antonio</creator><general>MDPI AG</general><general>Molecular Diversity Preservation International (MDPI)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20130408</creationdate><title>Melatonin inhibits GnRH-1, GnRH-3 and GnRH receptor expression in the brain of the European Sea Bass, Dicentrarchus labrax</title><author>Servili, Arianna ; Herrera-Pérez, Patricia ; Del Carmen Rendón, María ; Muñoz-Cueto, José Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-652abe7c59166978a3bb33822983b2ad1a15c482eb0e63b6d9e8e90d189694ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Bass</topic><topic>Bass - metabolism</topic><topic>Binding sites</topic><topic>Brain - metabolism</topic><topic>College campuses</topic><topic>Female</topic><topic>Fish Proteins - biosynthesis</topic><topic>Gene Expression Regulation - physiology</topic><topic>Hormones</topic><topic>Male</topic><topic>Melatonin</topic><topic>Melatonin - metabolism</topic><topic>Photoperiod</topic><topic>Physiology</topic><topic>Pituitary gland</topic><topic>Receptors, LHRH - biosynthesis</topic><topic>Reproduction - physiology</topic><topic>Seasonal variations</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Servili, Arianna</creatorcontrib><creatorcontrib>Herrera-Pérez, Patricia</creatorcontrib><creatorcontrib>Del Carmen Rendón, María</creatorcontrib><creatorcontrib>Muñoz-Cueto, José Antonio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Servili, Arianna</au><au>Herrera-Pérez, Patricia</au><au>Del Carmen Rendón, María</au><au>Muñoz-Cueto, José Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Melatonin inhibits GnRH-1, GnRH-3 and GnRH receptor expression in the brain of the European Sea Bass, Dicentrarchus labrax</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2013-04-08</date><risdate>2013</risdate><volume>14</volume><issue>4</issue><spage>7603</spage><epage>7616</epage><pages>7603-7616</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Several evidences supported the existence of melatonin effects on reproductive system in fish. In order to investigate whether melatonin is involved in the modulation of GnRH systems in the European sea bass, we have injected melatonin (0.5 µg/g body mass) in male specimens. The brain mRNA transcript levels of the three GnRH forms and the five GnRH receptors present in this species were determined by real time quantitative PCR. Our findings revealed day-night variations in the brain expression of GnRH-1, GnRH-3 and several GnRH receptors (dlGnRHR-II-1c, -2a), which exhibited higher transcript levels at mid-light compared to mid-dark phase of the photocycle. Moreover, an inhibitory effect of melatonin on the nocturnal expression of GnRH-1, GnRH-3, and GnRH receptors subtypes 1c, 2a and 2b was also demonstrated. Interestingly, the inhibitory effect of melatonin affected the expression of hypophysiotrophic GnRH forms and GnRH receptors that exhibit day-night fluctuations, suggesting that exogenous melatonin reinforce physiological mechanisms already established. These interactions between melatoninergic and GnRH systems could be mediating photoperiod effects on reproductive and other rhythmic physiological events in the European sea bass.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>23567273</pmid><doi>10.3390/ijms14047603</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Bass Bass - metabolism Binding sites Brain - metabolism College campuses Female Fish Proteins - biosynthesis Gene Expression Regulation - physiology Hormones Male Melatonin Melatonin - metabolism Photoperiod Physiology Pituitary gland Receptors, LHRH - biosynthesis Reproduction - physiology Seasonal variations |
| title | Melatonin inhibits GnRH-1, GnRH-3 and GnRH receptor expression in the brain of the European Sea Bass, Dicentrarchus labrax |
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