Regioselective Covalent Immobilization of Catalytically Active Glutathione S‑Transferase on Glass Slides

The high selectivity of protein farnesyltransferase was used to regioselectively append farnesyl analogues bearing bioorthogonal alkyne and azide functional groups to recombinant Schistosoma japonicum glutathione S-transferase (GSTase) and the active modified protein was covalently attached to glass...

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Veröffentlicht in:	Bioconjugate chemistry 2013-04, Vol.24 (4), p.571-577
Hauptverfasser:	Viswanathan, Rajesh, Labadie, Guillermo R, Poulter, C. Dale
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Azide Biocatalysis Biochemistry Chemical bonds E coli Enzyme Activation Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Glass - chemistry Glutathione Transferase - chemistry Glutathione Transferase - metabolism Models, Molecular Molecular Structure Parasites Polypeptides Proteins Recombinant Proteins - chemistry Recombinant Proteins - metabolism Schistosoma japonicum - enzymology Stereoisomerism Surface Properties Wells Yeasts
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